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P22007 (FNTB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein farnesyltransferase subunit beta
Short name=FTase-beta
EC=2.5.1.58
Alternative name(s):
CAAX farnesyltransferase subunit beta
Ras proteins prenyltransferase subunit beta
Gene names
Name:RAM1
Synonyms:DPR1, SCG2, STE16
Ordered Locus Names:YDL090C
ORF Names:D2412
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length431 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins such as a-factor and RAS. The beta subunit is responsible for peptide-binding.

Catalytic activity

Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Heterodimer of an alpha and a beta subunit.

Miscellaneous

Present with 3910 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein prenyltransferase subunit beta family.

Contains 5 PFTB repeats.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RAM2P297034EBI-14806,EBI-14814

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 431431Protein farnesyltransferase subunit beta
PRO_0000119768

Regions

Repeat130 – 17142PFTB 1
Repeat182 – 22443PFTB 2
Repeat231 – 27343PFTB 3
Repeat280 – 32243PFTB 4
Repeat332 – 37544PFTB 5

Sites

Metal binding3071Zinc By similarity
Metal binding3091Zinc By similarity
Metal binding3631Zinc By similarity

Amino acid modifications

Modified residue171Phosphothreonine Ref.7
Modified residue381Phosphoserine Ref.7

Experimental info

Sequence conflict471K → R in AAT92990. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P22007 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 2E3B64F30D2FF13A

FASTA43148,190
        10         20         30         40         50         60 
MRQRVGRSIA RAKFINTALL GRKRPVMERV VDIAHVDSSK AIQPLMKELE TDTTEARYKV 

        70         80         90        100        110        120 
LQSVLEIYDD EKNIEPALTK EFHKMYLDVA FEISLPPQMT ALDASQPWML YWIANSLKVM 

       130        140        150        160        170        180 
DRDWLSDDTK RKIVDKLFTI SPSGGPFGGG PGQLSHLAST YAAINALSLC DNIDGCWDRI 

       190        200        210        220        230        240 
DRKGIYQWLI SLKEPNGGFK TCLEVGEVDT RGIYCALSIA TLLNILTEEL TEGVLNYLKN 

       250        260        270        280        290        300 
CQNYEGGFGS CPHVDEAHGG YTFCATASLA ILRSMDQINV EKLLEWSSAR QLQEERGFCG 

       310        320        330        340        350        360 
RSNKLVDGCY SFWVGGSAAI LEAFGYGQCF NKHALRDYIL YCCQEKEQPG LRDKPGAHSD 

       370        380        390        400        410        420 
FYHTNYCLLG LAVAESSYSC TPNDSPHNIK CTPDRLIGSS KLTDVNPVYG LPIENVRKII 

       430 
HYFKSNLSSP S

« Hide

References

« Hide 'large scale' references
[1]"Structure and expression of yeast DPR1, a gene essential for the processing and intracellular localization of ras proteins."
Goodman L.E., Perou C.M., Fujiyama A., Tamanoi F.
Yeast 4:271-281(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames."
Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A.
Yeast 12:1377-1384(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-17 AND SER-38, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M22753 Genomic DNA. Translation: AAA34579.1.
X95644 Genomic DNA. Translation: CAA64921.1.
Z74138 Genomic DNA. Translation: CAA98656.1.
AY692971 Genomic DNA. Translation: AAT92990.1.
BK006938 Genomic DNA. Translation: DAA11768.1.
PIRBVBYDP. S67626.
RefSeqNP_010193.1. NM_001180149.1.

3D structure databases

ProteinModelPortalP22007.
SMRP22007. Positions 49-408.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1556N.
IntActP22007. 9 interactions.
MINTMINT-396276.

Proteomic databases

PaxDbP22007.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL090C; YDL090C; YDL090C.
GeneID851468.
KEGGsce:YDL090C.

Organism-specific databases

CYGDYDL090c.
SGDS000002248. RAM1.

Phylogenomic databases

eggNOGCOG5029.
GeneTreeENSGT00550000075042.
HOGENOMHOG000190594.
KOK05954.
OMAYWIANSL.
OrthoDBEOG4ZPJ4B.

Gene expression databases

GenevestigatorP22007.
GermOnlineYDL090C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR026872. FTB.
IPR001330. Prenyltrans.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PANTHERPTHR11774:SF6. PTHR11774:SF6. 1 hit.
PfamPF00432. Prenyltrans. 5 hits.
[Graphical view]
SUPFAMSSF48239. Terp_cyc_toroid. 1 hit.
ProtoNetSearch...

Other

BindingDBP22007.
ChEMBLCHEMBL4122.
NextBio968756.

Entry information

Entry nameFNTB_YEAST
AccessionPrimary (citable) accession number: P22007
Secondary accession number(s): D6VRQ8, E9P902, Q12422
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents