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P22007

- FNTB_YEAST

UniProt

P22007 - FNTB_YEAST

Protein

Protein farnesyltransferase subunit beta

Gene

RAM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins such as a-factor and RAS. The beta subunit is responsible for peptide-binding.

    Catalytic activityi

    Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei108 – 1081Important for selectivity against geranylgeranyl diphosphateBy similarity
    Metal bindingi307 – 3071Zinc; catalyticBy similarity
    Metal bindingi309 – 3091Zinc; catalyticBy similarity
    Metal bindingi363 – 3631Zinc; via tele nitrogen; catalyticBy similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein farnesyltransferase activity Source: SGD
    3. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. protein farnesylation Source: SGD
    2. regulation of cell proliferation Source: InterPro

    Keywords - Molecular functioni

    Prenyltransferase, Transferase

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:MONOMER3O-269.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein farnesyltransferase subunit beta (EC:2.5.1.58)
    Short name:
    FTase-beta
    Alternative name(s):
    CAAX farnesyltransferase subunit beta
    Ras proteins prenyltransferase subunit beta
    Gene namesi
    Name:RAM1
    Synonyms:DPR1, SCG2, STE16
    Ordered Locus Names:YDL090C
    ORF Names:D2412
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL090c.
    SGDiS000002248. RAM1.

    Subcellular locationi

    GO - Cellular componenti

    1. protein farnesyltransferase complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 431431Protein farnesyltransferase subunit betaPRO_0000119768Add
    BLAST

    Proteomic databases

    MaxQBiP22007.
    PaxDbiP22007.

    Expressioni

    Gene expression databases

    GenevestigatoriP22007.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RAM2P297034EBI-14806,EBI-14814

    Protein-protein interaction databases

    BioGridi31970. 46 interactions.
    DIPiDIP-1556N.
    IntActiP22007. 10 interactions.
    MINTiMINT-396276.

    Structurei

    3D structure databases

    ProteinModelPortaliP22007.
    SMRiP22007. Positions 40-429.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati130 – 17142PFTB 1Add
    BLAST
    Repeati182 – 22443PFTB 2Add
    BLAST
    Repeati231 – 27343PFTB 3Add
    BLAST
    Repeati280 – 32243PFTB 4Add
    BLAST
    Repeati332 – 37544PFTB 5Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni258 – 2614Farnesyl diphosphate bindingBy similarity
    Regioni301 – 3044Farnesyl diphosphate bindingBy similarity
    Regioni310 – 3134Farnesyl diphosphate bindingBy similarity

    Sequence similaritiesi

    Contains 5 PFTB repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5029.
    GeneTreeiENSGT00550000075042.
    HOGENOMiHOG000190594.
    KOiK05954.
    OMAiDEAHGGY.
    OrthoDBiEOG7XSTQC.

    Family and domain databases

    Gene3Di1.50.10.20. 1 hit.
    InterProiIPR026872. FTB.
    IPR001330. Prenyltrans.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PANTHERiPTHR11774:SF6. PTHR11774:SF6. 1 hit.
    PfamiPF00432. Prenyltrans. 5 hits.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P22007-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRQRVGRSIA RAKFINTALL GRKRPVMERV VDIAHVDSSK AIQPLMKELE    50
    TDTTEARYKV LQSVLEIYDD EKNIEPALTK EFHKMYLDVA FEISLPPQMT 100
    ALDASQPWML YWIANSLKVM DRDWLSDDTK RKIVDKLFTI SPSGGPFGGG 150
    PGQLSHLAST YAAINALSLC DNIDGCWDRI DRKGIYQWLI SLKEPNGGFK 200
    TCLEVGEVDT RGIYCALSIA TLLNILTEEL TEGVLNYLKN CQNYEGGFGS 250
    CPHVDEAHGG YTFCATASLA ILRSMDQINV EKLLEWSSAR QLQEERGFCG 300
    RSNKLVDGCY SFWVGGSAAI LEAFGYGQCF NKHALRDYIL YCCQEKEQPG 350
    LRDKPGAHSD FYHTNYCLLG LAVAESSYSC TPNDSPHNIK CTPDRLIGSS 400
    KLTDVNPVYG LPIENVRKII HYFKSNLSSP S 431
    Length:431
    Mass (Da):48,190
    Last modified:November 1, 1997 - v2
    Checksum:i2E3B64F30D2FF13A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti47 – 471K → R in AAT92990. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22753 Genomic DNA. Translation: AAA34579.1.
    X95644 Genomic DNA. Translation: CAA64921.1.
    Z74138 Genomic DNA. Translation: CAA98656.1.
    AY692971 Genomic DNA. Translation: AAT92990.1.
    BK006938 Genomic DNA. Translation: DAA11768.1.
    PIRiS67626. BVBYDP.
    RefSeqiNP_010193.1. NM_001180149.1.

    Genome annotation databases

    EnsemblFungiiYDL090C; YDL090C; YDL090C.
    GeneIDi851468.
    KEGGisce:YDL090C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22753 Genomic DNA. Translation: AAA34579.1 .
    X95644 Genomic DNA. Translation: CAA64921.1 .
    Z74138 Genomic DNA. Translation: CAA98656.1 .
    AY692971 Genomic DNA. Translation: AAT92990.1 .
    BK006938 Genomic DNA. Translation: DAA11768.1 .
    PIRi S67626. BVBYDP.
    RefSeqi NP_010193.1. NM_001180149.1.

    3D structure databases

    ProteinModelPortali P22007.
    SMRi P22007. Positions 40-429.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31970. 46 interactions.
    DIPi DIP-1556N.
    IntActi P22007. 10 interactions.
    MINTi MINT-396276.

    Chemistry

    BindingDBi P22007.
    ChEMBLi CHEMBL2111393.

    Proteomic databases

    MaxQBi P22007.
    PaxDbi P22007.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL090C ; YDL090C ; YDL090C .
    GeneIDi 851468.
    KEGGi sce:YDL090C.

    Organism-specific databases

    CYGDi YDL090c.
    SGDi S000002248. RAM1.

    Phylogenomic databases

    eggNOGi COG5029.
    GeneTreei ENSGT00550000075042.
    HOGENOMi HOG000190594.
    KOi K05954.
    OMAi DEAHGGY.
    OrthoDBi EOG7XSTQC.

    Enzyme and pathway databases

    BioCyci YEAST:MONOMER3O-269.

    Miscellaneous databases

    NextBioi 968756.
    PROi P22007.

    Gene expression databases

    Genevestigatori P22007.

    Family and domain databases

    Gene3Di 1.50.10.20. 1 hit.
    InterProi IPR026872. FTB.
    IPR001330. Prenyltrans.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view ]
    PANTHERi PTHR11774:SF6. PTHR11774:SF6. 1 hit.
    Pfami PF00432. Prenyltrans. 5 hits.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of yeast DPR1, a gene essential for the processing and intracellular localization of ras proteins."
      Goodman L.E., Perou C.M., Fujiyama A., Tamanoi F.
      Yeast 4:271-281(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames."
      Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A.
      Yeast 12:1377-1384(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFNTB_YEAST
    AccessioniPrimary (citable) accession number: P22007
    Secondary accession number(s): D6VRQ8, E9P902, Q12422
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3910 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3