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Protein

Protein farnesyltransferase subunit beta

Gene

RAM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins such as a-factor and RAS. The beta subunit is responsible for peptide-binding.

Miscellaneous

Present with 3910 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei108Important for selectivity against geranylgeranyl diphosphateBy similarity1
Metal bindingi307Zinc; catalyticBy similarity1
Metal bindingi309Zinc; catalyticBy similarity1
Metal bindingi363Zinc; via tele nitrogen; catalyticBy similarity1

GO - Molecular functioni

  • protein farnesyltransferase activity Source: SGD
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • protein farnesylation Source: SGD

Keywordsi

Molecular functionPrenyltransferase, Transferase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-269

Names & Taxonomyi

Protein namesi
Recommended name:
Protein farnesyltransferase subunit beta (EC:2.5.1.58)
Short name:
FTase-beta
Alternative name(s):
CAAX farnesyltransferase subunit beta
Ras proteins prenyltransferase subunit beta
Gene namesi
Name:RAM1
Synonyms:DPR1, SCG2, STE16
Ordered Locus Names:YDL090C
ORF Names:D2412
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL090C
SGDiS000002248 RAM1

Subcellular locationi

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2111393

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197681 – 431Protein farnesyltransferase subunit betaAdd BLAST431

Proteomic databases

MaxQBiP22007
PaxDbiP22007
PRIDEiP22007

PTM databases

iPTMnetiP22007

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
RAM2P297033EBI-14806,EBI-14814

Protein-protein interaction databases

BioGridi31970, 242 interactors
DIPiDIP-1556N
IntActiP22007, 10 interactors
MINTiP22007
STRINGi4932.YDL090C

Chemistry databases

BindingDBiP22007

Structurei

3D structure databases

ProteinModelPortaliP22007
SMRiP22007
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati130 – 171PFTB 1Add BLAST42
Repeati182 – 224PFTB 2Add BLAST43
Repeati231 – 273PFTB 3Add BLAST43
Repeati280 – 322PFTB 4Add BLAST43
Repeati332 – 375PFTB 5Add BLAST44

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni258 – 261Farnesyl diphosphate bindingBy similarity4
Regioni301 – 304Farnesyl diphosphate bindingBy similarity4
Regioni310 – 313Farnesyl diphosphate bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000075042
HOGENOMiHOG000190594
InParanoidiP22007
KOiK05954
OMAiCYTAMAV
OrthoDBiEOG092C26Q9

Family and domain databases

CDDicd02893 FTase, 1 hit
InterProiView protein in InterPro
IPR026872 FTB
IPR001330 PFTB_repeat
IPR008930 Terpenoid_cyclase/PrenylTrfase
PANTHERiPTHR11774:SF6 PTHR11774:SF6, 1 hit
PfamiView protein in Pfam
PF00432 Prenyltrans, 5 hits
SUPFAMiSSF48239 SSF48239, 1 hit

Sequencei

Sequence statusi: Complete.

P22007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQRVGRSIA RAKFINTALL GRKRPVMERV VDIAHVDSSK AIQPLMKELE
60 70 80 90 100
TDTTEARYKV LQSVLEIYDD EKNIEPALTK EFHKMYLDVA FEISLPPQMT
110 120 130 140 150
ALDASQPWML YWIANSLKVM DRDWLSDDTK RKIVDKLFTI SPSGGPFGGG
160 170 180 190 200
PGQLSHLAST YAAINALSLC DNIDGCWDRI DRKGIYQWLI SLKEPNGGFK
210 220 230 240 250
TCLEVGEVDT RGIYCALSIA TLLNILTEEL TEGVLNYLKN CQNYEGGFGS
260 270 280 290 300
CPHVDEAHGG YTFCATASLA ILRSMDQINV EKLLEWSSAR QLQEERGFCG
310 320 330 340 350
RSNKLVDGCY SFWVGGSAAI LEAFGYGQCF NKHALRDYIL YCCQEKEQPG
360 370 380 390 400
LRDKPGAHSD FYHTNYCLLG LAVAESSYSC TPNDSPHNIK CTPDRLIGSS
410 420 430
KLTDVNPVYG LPIENVRKII HYFKSNLSSP S
Length:431
Mass (Da):48,190
Last modified:November 1, 1997 - v2
Checksum:i2E3B64F30D2FF13A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47K → R in AAT92990 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22753 Genomic DNA Translation: AAA34579.1
X95644 Genomic DNA Translation: CAA64921.1
Z74138 Genomic DNA Translation: CAA98656.1
AY692971 Genomic DNA Translation: AAT92990.1
BK006938 Genomic DNA Translation: DAA11768.1
PIRiS67626 BVBYDP
RefSeqiNP_010193.1, NM_001180149.1

Genome annotation databases

EnsemblFungiiYDL090C; YDL090C; YDL090C
GeneIDi851468
KEGGisce:YDL090C

Similar proteinsi

Entry informationi

Entry nameiFNTB_YEAST
AccessioniPrimary (citable) accession number: P22007
Secondary accession number(s): D6VRQ8, E9P902, Q12422
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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