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P22005

- PENK_MOUSE

UniProt

P22005 - PENK_MOUSE

Protein

Proenkephalin-A

Gene

Penk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(238-259) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.

    GO - Biological processi

    1. aggressive behavior Source: MGI
    2. behavioral fear response Source: MGI
    3. locomotory behavior Source: MGI
    4. neuropeptide signaling pathway Source: UniProtKB-KW
    5. sensory perception of pain Source: MGI
    6. startle response Source: MGI

    Keywords - Molecular functioni

    Endorphin, Neuropeptide, Opioid peptide

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proenkephalin-A
    Cleaved into the following 8 chains:
    Gene namesi
    Name:Penk
    Synonyms:Penk1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:104629. Penk.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Add
    BLAST
    Peptidei25 – 9773SynenkephalinPRO_0000008268Add
    BLAST
    Peptidei100 – 1045Met-enkephalinPRO_0000008269
    Peptidei107 – 1115Met-enkephalinPRO_0000008270
    Peptidei114 – 13320PENK(114-133)By similarityPRO_0000377694Add
    BLAST
    Peptidei136 – 1405Met-enkephalinPRO_0000008272
    Peptidei143 – 18442PENK(143-184)By similarityPRO_0000377695Add
    BLAST
    Peptidei187 – 1948Met-enkephalin-Arg-Ser-LeuPRO_0000008274
    Propeptidei197 – 20812PRO_0000008275Add
    BLAST
    Peptidei211 – 2155Met-enkephalinPRO_0000008276
    Propeptidei218 – 22811PRO_0000008277Add
    BLAST
    Peptidei231 – 2355Leu-enkephalinPRO_0000008278
    Peptidei238 – 25922PENK(238-259)By similarityPRO_0000377696Add
    BLAST
    Peptidei262 – 2687Met-enkephalin-Arg-PhePRO_0000008280

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 48By similarity
    Disulfide bondi30 ↔ 52By similarity
    Disulfide bondi33 ↔ 65By similarity

    Post-translational modificationi

    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Proteomic databases

    MaxQBiP22005.
    PaxDbiP22005.
    PRIDEiP22005.

    PTM databases

    PhosphoSiteiP22005.

    Expressioni

    Tissue specificityi

    Spermatogenic and somatic cells.

    Developmental stagei

    Highest expression in late pachytene spermatocytes and postmeiotic round spermatids.

    Gene expression databases

    ArrayExpressiP22005.
    BgeeiP22005.
    CleanExiMM_PENK1.
    GenevestigatoriP22005.

    Interactioni

    Protein-protein interaction databases

    BioGridi202109. 1 interaction.
    IntActiP22005. 1 interaction.
    MINTiMINT-4107012.

    Structurei

    3D structure databases

    ProteinModelPortaliP22005.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG40630.
    GeneTreeiENSGT00530000063761.
    HOGENOMiHOG000013003.
    HOVERGENiHBG000063.
    InParanoidiQ68G73.
    OMAiGGFMKKD.
    OrthoDBiEOG75TMCR.
    PhylomeDBiP22005.
    TreeFamiTF332620.

    Family and domain databases

    InterProiIPR006024. Opioid_neupept.
    IPR000703. Proenkphlin_A.
    [Graphical view]
    PANTHERiPTHR11438. PTHR11438. 1 hit.
    PTHR11438:SF3. PTHR11438:SF3. 1 hit.
    PfamiPF01160. Opiods_neuropep. 1 hit.
    [Graphical view]
    PRINTSiPR01028. OPIOIDPRCRSR.
    PR01029. PENKAPRCRSR.
    PROSITEiPS01252. OPIOIDS_PRECURSOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22005-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARFLRLCTW LLALGSCLLA TVQAECSQDC AKCSYRLVRP GDINFLACTL    50
    ECEGQLPSFK IWETCKDLLQ VSRPEFPWDN IDMYKDSSKQ DESHLLAKKY 100
    GGFMKRYGGF MKKMDELYPM EPEEEANGGE ILAKRYGGFM KKDADEGDTL 150
    ANSSDLLKEL LGTGDNRAKD SHQQESTNND EDMSKRYGGF MRSLKRSPQL 200
    EDEAKELQKR YGGFMRRVGR PEWWMDYQKR YGGFLKRFAE SLPSDEEGEN 250
    YSKEVPEIEK RYGGFMRF 268
    Length:268
    Mass (Da):31,004
    Last modified:June 16, 2009 - v2
    Checksum:i637F848B49F44013
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 341S → T in AAA40128. (PubMed:2355920)Curated
    Sequence conflicti184 – 1841S → SS in AAA40128. (PubMed:2355920)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55181 mRNA. Translation: AAA40128.1.
    AK161272 mRNA. Translation: BAE36283.1.
    BX004841 Genomic DNA. Translation: CAM26883.1.
    CH466538 Genomic DNA. Translation: EDL05699.1.
    CH466538 Genomic DNA. Translation: EDL05700.1.
    BC049766 mRNA. Translation: AAH49766.1.
    M13227 mRNA. Translation: AAA37553.1.
    CCDSiCCDS17946.1.
    PIRiB35678.
    RefSeqiNP_001002927.1. NM_001002927.2.
    XP_006537738.1. XM_006537675.1.
    UniGeneiMm.475097.

    Genome annotation databases

    EnsembliENSMUST00000070375; ENSMUSP00000066822; ENSMUSG00000045573.
    GeneIDi18619.
    KEGGimmu:18619.
    UCSCiuc008rwz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55181 mRNA. Translation: AAA40128.1 .
    AK161272 mRNA. Translation: BAE36283.1 .
    BX004841 Genomic DNA. Translation: CAM26883.1 .
    CH466538 Genomic DNA. Translation: EDL05699.1 .
    CH466538 Genomic DNA. Translation: EDL05700.1 .
    BC049766 mRNA. Translation: AAH49766.1 .
    M13227 mRNA. Translation: AAA37553.1 .
    CCDSi CCDS17946.1.
    PIRi B35678.
    RefSeqi NP_001002927.1. NM_001002927.2.
    XP_006537738.1. XM_006537675.1.
    UniGenei Mm.475097.

    3D structure databases

    ProteinModelPortali P22005.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202109. 1 interaction.
    IntActi P22005. 1 interaction.
    MINTi MINT-4107012.

    PTM databases

    PhosphoSitei P22005.

    Proteomic databases

    MaxQBi P22005.
    PaxDbi P22005.
    PRIDEi P22005.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000070375 ; ENSMUSP00000066822 ; ENSMUSG00000045573 .
    GeneIDi 18619.
    KEGGi mmu:18619.
    UCSCi uc008rwz.1. mouse.

    Organism-specific databases

    CTDi 5179.
    MGIi MGI:104629. Penk.

    Phylogenomic databases

    eggNOGi NOG40630.
    GeneTreei ENSGT00530000063761.
    HOGENOMi HOG000013003.
    HOVERGENi HBG000063.
    InParanoidi Q68G73.
    OMAi GGFMKKD.
    OrthoDBi EOG75TMCR.
    PhylomeDBi P22005.
    TreeFami TF332620.

    Miscellaneous databases

    NextBioi 294568.
    PROi P22005.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P22005.
    Bgeei P22005.
    CleanExi MM_PENK1.
    Genevestigatori P22005.

    Family and domain databases

    InterProi IPR006024. Opioid_neupept.
    IPR000703. Proenkphlin_A.
    [Graphical view ]
    PANTHERi PTHR11438. PTHR11438. 1 hit.
    PTHR11438:SF3. PTHR11438:SF3. 1 hit.
    Pfami PF01160. Opiods_neuropep. 1 hit.
    [Graphical view ]
    PRINTSi PR01028. OPIOIDPRCRSR.
    PR01029. PENKAPRCRSR.
    PROSITEi PS01252. OPIOIDS_PRECURSOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transcription of the rat and mouse proenkephalin genes is initiated at distinct sites in spermatogenic and somatic cells."
      Kilpatrick D.L., Zinn S.A., Fitzgerald M., Higuchi H., Sabol S.L., Meyerhardt J.
      Mol. Cell. Biol. 10:3717-3726(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    6. "Activation of mouse T-helper cells induces abundant preproenkephalin mRNA synthesis."
      Zurawski G., Benedik M., Kamb B.J., Abrams J.S., Zurawski S.M., Lee F.D.
      Science 232:772-775(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-268.
      Tissue: T-cell.

    Entry informationi

    Entry nameiPENK_MOUSE
    AccessioniPrimary (citable) accession number: P22005
    Secondary accession number(s): Q68G73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3