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P22001 (KCNA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 150. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily A member 3
Alternative name(s):
HGK5
HLK3
HPCN3
Voltage-gated K(+) channel HuKIII
Voltage-gated potassium channel subunit Kv1.3
Gene names
Name:KCNA3
Synonyms:HGK5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length575 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

Subunit structure

Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 By similarity.

Subcellular location

Cell membrane By similarity; Multi-pass membrane protein.

Domain

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Post-translational modification

N-glycosylation promotes the cell surface expression By similarity.

Sequence similarities

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.3/KCNA3 sub-subfamily. [View classification]

Caution

It is uncertain whether Met-1 or Met-53 is the initiator.

Sequence caution

The sequence AAA36425.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAA36425.1 differs from that shown. Reason: Frameshift at position 42.

The sequence AAA59457.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAA59457.1 differs from that shown. Reason: Frameshift at several positions.

The sequence AAB88073.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAC31761.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAC31761.1 differs from that shown. Reason: Frameshift at several positions.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 575575Potassium voltage-gated channel subfamily A member 3
PRO_0000053977

Regions

Topological domain1 – 234234Cytoplasmic Potential
Transmembrane235 – 25319Helical; Name=Segment S1; Potential
Topological domain254 – 29441Extracellular Potential
Transmembrane295 – 31622Helical; Name=Segment S2; Potential
Topological domain317 – 32711Cytoplasmic Potential
Transmembrane328 – 34821Helical; Name=Segment S3; Potential
Topological domain349 – 36214Extracellular Potential
Transmembrane363 – 38119Helical; Voltage-sensor; Name=Segment S4; Potential
Topological domain382 – 39716Cytoplasmic Potential
Transmembrane398 – 41720Helical; Name=Segment S5; Potential
Topological domain418 – 45841Extracellular Potential
Transmembrane459 – 48123Helical; Name=Segment S6; Potential
Topological domain482 – 57594Cytoplasmic Potential
Motif444 – 4496Selectivity filter By similarity
Motif573 – 5753PDZ-binding By similarity

Amino acid modifications

Modified residue5201Phosphoserine; by PKA Potential
Lipidation3171S-palmitoyl cysteine Potential
Glycosylation2791N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict721A → G in AAA36425. Ref.5
Sequence conflict891L → V in AAA36425. Ref.5
Sequence conflict1131L → V in AAA59457. Ref.6
Sequence conflict1431L → V in AAA36425. Ref.5
Sequence conflict3091E → K in AAC31761. Ref.1
Sequence conflict3901T → S in AAA36425. Ref.5
Sequence conflict4711T → S in AAA36425. Ref.5
Sequence conflict5401S → T in AAA36425. Ref.5

Secondary structure

.................... 575
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22001 [UniParc].

Last modified November 25, 2008. Version 3.
Checksum: 0E98905187A85F48

FASTA57563,842
        10         20         30         40         50         60 
MDERLSLLRS PPPPSARHRA HPPQRPASSG GAHTLVNHGY AEPAAGRELP PDMTVVPGDH 

        70         80         90        100        110        120 
LLEPEVADGG GAPPQGGCGG GGCDRYEPLP PSLPAAGEQD CCGERVVINI SGLRFETQLK 

       130        140        150        160        170        180 
TLCQFPETLL GDPKRRMRYF DPLRNEYFFD RNRPSFDAIL YYYQSGGRIR RPVNVPIDIF 

       190        200        210        220        230        240 
SEEIRFYQLG EEAMEKFRED EGFLREEERP LPRRDFQRQV WLLFEYPESS GPARGIAIVS 

       250        260        270        280        290        300 
VLVILISIVI FCLETLPEFR DEKDYPASTS QDSFEAAGNS TSGSRAGASS FSDPFFVVET 

       310        320        330        340        350        360 
LCIIWFSFEL LVRFFACPSK ATFSRNIMNL IDIVAIIPYF ITLGTELAER QGNGQQAMSL 

       370        380        390        400        410        420 
AILRVIRLVR VFRIFKLSRH SKGLQILGQT LKASMRELGL LIFFLFIGVI LFSSAVYFAE 

       430        440        450        460        470        480 
ADDPTSGFSS IPDAFWWAVV TMTTVGYGDM HPVTIGGKIV GSLCAIAGVL TIALPVPVIV 

       490        500        510        520        530        540 
SNFNYFYHRE TEGEEQSQYM HVGSCQHLSS SAEELRKARS NSTLSKSEYM VIEEGGMNHS 

       550        560        570 
AFPQTPFKTG NSTATCTTNN NPNSCVNIKK IFTDV 

« Hide

References

« Hide 'large scale' references
[1]"Confirmation of the assignment of the gene encoding Kv1.3, a voltage-gated potassium channel (KCNA3) to the proximal short arm of human chromosome 1."
Folander K., Douglass J., Swanson R.
Genomics 23:295-296(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Blood.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Sequence and functional expression in Xenopus oocytes of a human insulinoma and islet potassium channel."
Philipson L.H., Hice R.E., Schaefer K., Lamendola J., Bell G.I., Nelson D.J., Steiner D.F.
Proc. Natl. Acad. Sci. U.S.A. 88:53-57(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-575.
Tissue: Skeletal muscle.
[6]"Cloning, functional expression, and regulation of two K+ channels in human T lymphocytes."
Attali B., Romey G., Honore E., Schmid-Alliana A., Mattei M.-G., Lesage F., Ricard P., Barhanin J., Lazdunski M.
J. Biol. Chem. 267:8650-8657(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-575.
[7]"Characterization and functional expression of genomic DNA encoding the human lymphocyte type n potassium channel."
Cai Y.-C., Osborne P.B., North R.A., Dooley D.C., Douglass J.
DNA Cell Biol. 11:163-172(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 47-575.
Tissue: Lymphocyte.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23499 mRNA. Translation: AAC31761.1. Sequence problems.
AL365361 Genomic DNA. Translation: CAH71983.1.
CH471122 Genomic DNA. Translation: EAW56457.1.
BC035059 mRNA. Translation: AAH35059.2.
M55515 Genomic DNA. Translation: AAA36425.1. Sequence problems.
M85217 mRNA. Translation: AAA59457.1. Sequence problems.
M38217 Genomic DNA. Translation: AAB88073.1. Different initiation.
CCDSCCDS828.2.
PIRA38101.
RefSeqNP_002223.3. NM_002232.4.
UniGeneHs.169948.
Hs.619197.
Hs.628223.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BGCX-ray1.20A104-204[»]
ProteinModelPortalP22001.
SMRP22001. Positions 104-491.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109941. 11 interactions.
DIPDIP-44822N.
MINTMINT-1528422.
STRING9606.ENSP00000358784.

Chemistry

BindingDBP22001.
ChEMBLCHEMBL4633.
GuidetoPHARMACOLOGY540.

Protein family/group databases

TCDB1.A.1.2.4. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteP22001.

Polymorphism databases

DMDM215274135.

Proteomic databases

MaxQBP22001.
PaxDbP22001.
PRIDEP22001.

Protocols and materials databases

DNASU3738.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000369769; ENSP00000358784; ENSG00000177272.
GeneID3738.
KEGGhsa:3738.
UCSCuc001dzv.1. human.

Organism-specific databases

CTD3738.
GeneCardsGC01M111214.
HGNCHGNC:6221. KCNA3.
HPAHPA016625.
MIM176263. gene.
neXtProtNX_P22001.
PharmGKBPA30021.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1226.
HOGENOMHOG000231015.
HOVERGENHBG052230.
InParanoidP22001.
KOK04876.
OMAKRRMRYF.
OrthoDBEOG7M0NRD.
PhylomeDBP22001.
TreeFamTF313103.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

ArrayExpressP22001.
BgeeP22001.
CleanExHS_KCNA3.
GenevestigatorP22001.

Family and domain databases

Gene3D1.20.120.350. 1 hit.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR003091. K_chnl.
IPR003968. K_chnl_volt-dep_Kv.
IPR003972. K_chnl_volt-dep_Kv1.
IPR004050. K_chnl_volt-dep_Kv1.3.
IPR003131. T1-type_BTB.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERPTHR11537. PTHR11537. 1 hit.
PfamPF02214. BTB_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSPR00169. KCHANNEL.
PR01510. KV13CHANNEL.
PR01491. KVCHANNEL.
PR01496. SHAKERCHANEL.
SMARTSM00225. BTB. 1 hit.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
ProtoNetSearch...

Other

GeneWikiKCNA3.
GenomeRNAi3738.
NextBio14631.
PROP22001.
SOURCESearch...

Entry information

Entry nameKCNA3_HUMAN
AccessionPrimary (citable) accession number: P22001
Secondary accession number(s): Q5VWN2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 25, 2008
Last modified: July 9, 2014
This is version 150 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM