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Protein

Embigin

Gene

Emb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in targeting the monocarboxylate transporters SLC16A1 and SLC16A7 to the cell membrane (By similarity). Plays a role in the outgrowth of motoneurons and in the formation of neuromuscular junctions. Following muscle denervation, promotes nerve terminal sprouting and the formation of additional acetylcholine receptor clusters at synaptic sites without affecting terminal Schwann cell number or morphology. Delays the retraction of terminal sprouts following re-innervation of denervated endplates.By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-433692. Proton-coupled monocarboxylate transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Embigin
Alternative name(s):
Teratocarcinoma glycoprotein Gp-70
Gene namesi
Name:Emb
Synonyms:Gp70
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:95321. Emb.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 254ExtracellularSequence analysisAdd BLAST221
Transmembranei255 – 283HelicalSequence analysisAdd BLAST29
Topological domaini284 – 330CytoplasmicSequence analysisAdd BLAST47

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000001475034 – 330EmbiginAdd BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi55N-linked (GlcNAc...)2 Publications1
Glycosylationi62N-linked (GlcNAc...)1 Publication1
Glycosylationi70N-linked (GlcNAc...)1 Publication1
Glycosylationi76N-linked (GlcNAc...); atypical2 Publications1
Disulfide bondi89 ↔ 145PROSITE-ProRule annotation
Glycosylationi98N-linked (GlcNAc...); atypical1 Publication1
Glycosylationi101N-linked (GlcNAc...)2 Publications1
Glycosylationi118N-linked (GlcNAc...)2 Publications1
Disulfide bondi182 ↔ 240PROSITE-ProRule annotation
Glycosylationi191N-linked (GlcNAc...)Sequence analysis1
Glycosylationi198N-linked (GlcNAc...)2 Publications1
Glycosylationi210N-linked (GlcNAc...); atypical1 Publication1
Glycosylationi216N-linked (GlcNAc...)2 Publications1
Glycosylationi221N-linked (GlcNAc...)2 Publications1
Modified residuei312PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP21995.
MaxQBiP21995.
PaxDbiP21995.
PeptideAtlasiP21995.
PRIDEiP21995.

PTM databases

iPTMnetiP21995.
PhosphoSitePlusiP21995.
SwissPalmiP21995.

Expressioni

Tissue specificityi

Only member of the immunoglobulin superfamily to be expressed in embryonal carcinoma cells, which resemble multipotential cells of early embryos.1 Publication

Developmental stagei

At neuromuscular junctions, 5-fold higher expression levels at P0 compared to adult.1 Publication

Inductioni

Regulated by muscle activity. Strongly up-regulated after muscle denervation, including that of gastrocnemius muscle. Maximal expression is observed 10 days after denervation (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000021728.
CleanExiMM_EMB.
GenevisibleiP21995. MM.

Interactioni

Subunit structurei

Interacts with SLC16A1 and SLC16A7.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022242.

Structurei

3D structure databases

ProteinModelPortaliP21995.
SMRiP21995.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 161Ig-like V-type 1Add BLAST124
Domaini162 – 256Ig-like V-type 2Add BLAST95

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVBC. Eukaryota.
ENOG41120A3. LUCA.
GeneTreeiENSGT00390000010516.
HOGENOMiHOG000082417.
HOVERGENiHBG051471.
InParanoidiP21995.
OMAiSKQMGSY.
OrthoDBiEOG091G0RFU.
PhylomeDBiP21995.
TreeFamiTF326759.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR027114. Embigin.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
[Graphical view]
PANTHERiPTHR10075:SF4. PTHR10075:SF4. 1 hit.
PfamiPF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21995-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSHTGLRAL VAPGYPLLLL CLLAATRPDP AEGDPTDPTF TSLPVREEMM
60 70 80 90 100
AKYSNLSLKS CNISVTEKSN VSVEENVILE KPSHVELKCV YTATKDLNLM
110 120 130 140 150
NVTWKKDDEP LETTGDFNTT KMGNTLTSQY RFIVFNSKQL GKYSCVFGEK
160 170 180 190 200
ELRGTFNIHV PKAHGKKKSL IAYVGDSTVL KCVCQDCLPL NWTWYMGNET
210 220 230 240 250
AQVPIDAHSN EKYIINGSHA NETRLKIKHL LEEDGGSYWC RATFQLGESE
260 270 280 290 300
EQNELVVLSF LVPLKPFLAI LAEVILLVAI ILLCEVYTHK KKNDPDAGKE
310 320 330
FEQIEQLKSD DSNGIENNVP RYRKTDSADQ
Length:330
Mass (Da):37,064
Last modified:February 1, 2005 - v2
Checksum:iFC4D729A993A1EDD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78I → S in BAC40381 (PubMed:16141072).Curated1
Sequence conflicti98N → S in BAC37687 (PubMed:16141072).Curated1
Sequence conflicti99L → S in BAC40381 (PubMed:16141072).Curated1
Sequence conflicti162 – 166KAHGK → QSSWE in AAA37730 (PubMed:2963822).Curated5
Sequence conflicti294D → G in AAA37730 (PubMed:2963822).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03535 mRNA. Translation: AAA37730.1.
AK079570 mRNA. Translation: BAC37687.1.
AK088480 mRNA. Translation: BAC40381.1.
AK138271 mRNA. Translation: BAE23603.1.
AK148540 mRNA. Translation: BAE28610.1.
AK150625 mRNA. Translation: BAE29715.1.
AK166602 mRNA. Translation: BAE38887.1.
BC014858 mRNA. Translation: AAH14858.1.
CCDSiCCDS26791.1.
PIRiA29915.
RefSeqiNP_034460.3. NM_010330.4.
UniGeneiMm.274926.

Genome annotation databases

EnsembliENSMUST00000022242; ENSMUSP00000022242; ENSMUSG00000021728.
GeneIDi13723.
KEGGimmu:13723.
UCSCiuc007rym.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03535 mRNA. Translation: AAA37730.1.
AK079570 mRNA. Translation: BAC37687.1.
AK088480 mRNA. Translation: BAC40381.1.
AK138271 mRNA. Translation: BAE23603.1.
AK148540 mRNA. Translation: BAE28610.1.
AK150625 mRNA. Translation: BAE29715.1.
AK166602 mRNA. Translation: BAE38887.1.
BC014858 mRNA. Translation: AAH14858.1.
CCDSiCCDS26791.1.
PIRiA29915.
RefSeqiNP_034460.3. NM_010330.4.
UniGeneiMm.274926.

3D structure databases

ProteinModelPortaliP21995.
SMRiP21995.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022242.

PTM databases

iPTMnetiP21995.
PhosphoSitePlusiP21995.
SwissPalmiP21995.

Proteomic databases

EPDiP21995.
MaxQBiP21995.
PaxDbiP21995.
PeptideAtlasiP21995.
PRIDEiP21995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022242; ENSMUSP00000022242; ENSMUSG00000021728.
GeneIDi13723.
KEGGimmu:13723.
UCSCiuc007rym.2. mouse.

Organism-specific databases

CTDi133418.
MGIiMGI:95321. Emb.

Phylogenomic databases

eggNOGiENOG410IVBC. Eukaryota.
ENOG41120A3. LUCA.
GeneTreeiENSGT00390000010516.
HOGENOMiHOG000082417.
HOVERGENiHBG051471.
InParanoidiP21995.
OMAiSKQMGSY.
OrthoDBiEOG091G0RFU.
PhylomeDBiP21995.
TreeFamiTF326759.

Enzyme and pathway databases

ReactomeiR-MMU-433692. Proton-coupled monocarboxylate transport.

Miscellaneous databases

PROiP21995.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021728.
CleanExiMM_EMB.
GenevisibleiP21995. MM.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiIPR027114. Embigin.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
[Graphical view]
PANTHERiPTHR10075:SF4. PTHR10075:SF4. 1 hit.
PfamiPF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMB_MOUSE
AccessioniPrimary (citable) accession number: P21995
Secondary accession number(s): Q3UFF1
, Q8C2J8, Q8C543, Q96C38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 2005
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.