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Protein

Embigin

Gene

Emb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in targeting the monocarboxylate transporters SLC16A1 and SLC16A7 to the cell membrane (By similarity). Plays a role in the outgrowth of motoneurons and in the formation of neuromuscular junctions. Following muscle denervation, promotes nerve terminal sprouting and the formation of additional acetylcholine receptor clusters at synaptic sites without affecting terminal Schwann cell number or morphology. Delays the retraction of terminal sprouts following re-innervation of denervated endplates.By similarity1 Publication

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-433692 Proton-coupled monocarboxylate transport

Names & Taxonomyi

Protein namesi
Recommended name:
Embigin
Alternative name(s):
Teratocarcinoma glycoprotein Gp-70
Gene namesi
Name:Emb
Synonyms:Gp70
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:95321 Emb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 254ExtracellularSequence analysisAdd BLAST221
Transmembranei255 – 283HelicalSequence analysisAdd BLAST29
Topological domaini284 – 330CytoplasmicSequence analysisAdd BLAST47

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000001475034 – 330EmbiginAdd BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi55N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi62N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi70N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi76N-linked (GlcNAc...) asparagine; atypical2 Publications1
Disulfide bondi89 ↔ 145PROSITE-ProRule annotation
Glycosylationi98N-linked (GlcNAc...) asparagine; atypical1 Publication1
Glycosylationi101N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi118N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi182 ↔ 240PROSITE-ProRule annotation
Glycosylationi191N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi198N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi210N-linked (GlcNAc...) asparagine; atypical1 Publication1
Glycosylationi216N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi221N-linked (GlcNAc...) asparagine2 Publications1
Modified residuei312PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP21995
MaxQBiP21995
PaxDbiP21995
PeptideAtlasiP21995
PRIDEiP21995

PTM databases

iPTMnetiP21995
PhosphoSitePlusiP21995
SwissPalmiP21995

Expressioni

Tissue specificityi

Only member of the immunoglobulin superfamily to be expressed in embryonal carcinoma cells, which resemble multipotential cells of early embryos.1 Publication

Developmental stagei

At neuromuscular junctions, 5-fold higher expression levels at P0 compared to adult.1 Publication

Inductioni

Regulated by muscle activity. Strongly up-regulated after muscle denervation, including that of gastrocnemius muscle. Maximal expression is observed 10 days after denervation (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000021728
CleanExiMM_EMB
GenevisibleiP21995 MM

Interactioni

Subunit structurei

Interacts with SLC16A1 and SLC16A7.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022242

Structurei

3D structure databases

ProteinModelPortaliP21995
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 161Ig-like V-type 1Add BLAST124
Domaini162 – 256Ig-like V-type 2Add BLAST95

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVBC Eukaryota
ENOG41120A3 LUCA
GeneTreeiENSGT00390000010516
HOGENOMiHOG000082417
HOVERGENiHBG051471
InParanoidiP21995
OMAiSKQMGSY
OrthoDBiEOG091G0RFU
PhylomeDBiP21995
TreeFamiTF326759

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR027114 Embigin
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
PANTHERiPTHR10075:SF4 PTHR10075:SF4, 1 hit
PfamiView protein in Pfam
PF07679 I-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 2 hits
SUPFAMiSSF48726 SSF48726, 2 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21995-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSHTGLRAL VAPGYPLLLL CLLAATRPDP AEGDPTDPTF TSLPVREEMM
60 70 80 90 100
AKYSNLSLKS CNISVTEKSN VSVEENVILE KPSHVELKCV YTATKDLNLM
110 120 130 140 150
NVTWKKDDEP LETTGDFNTT KMGNTLTSQY RFIVFNSKQL GKYSCVFGEK
160 170 180 190 200
ELRGTFNIHV PKAHGKKKSL IAYVGDSTVL KCVCQDCLPL NWTWYMGNET
210 220 230 240 250
AQVPIDAHSN EKYIINGSHA NETRLKIKHL LEEDGGSYWC RATFQLGESE
260 270 280 290 300
EQNELVVLSF LVPLKPFLAI LAEVILLVAI ILLCEVYTHK KKNDPDAGKE
310 320 330
FEQIEQLKSD DSNGIENNVP RYRKTDSADQ
Length:330
Mass (Da):37,064
Last modified:February 1, 2005 - v2
Checksum:iFC4D729A993A1EDD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78I → S in BAC40381 (PubMed:16141072).Curated1
Sequence conflicti98N → S in BAC37687 (PubMed:16141072).Curated1
Sequence conflicti99L → S in BAC40381 (PubMed:16141072).Curated1
Sequence conflicti162 – 166KAHGK → QSSWE in AAA37730 (PubMed:2963822).Curated5
Sequence conflicti294D → G in AAA37730 (PubMed:2963822).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03535 mRNA Translation: AAA37730.1
AK079570 mRNA Translation: BAC37687.1
AK088480 mRNA Translation: BAC40381.1
AK138271 mRNA Translation: BAE23603.1
AK148540 mRNA Translation: BAE28610.1
AK150625 mRNA Translation: BAE29715.1
AK166602 mRNA Translation: BAE38887.1
BC014858 mRNA Translation: AAH14858.1
CCDSiCCDS26791.1
PIRiA29915
RefSeqiNP_034460.3, NM_010330.4
UniGeneiMm.274926

Genome annotation databases

EnsembliENSMUST00000022242; ENSMUSP00000022242; ENSMUSG00000021728
GeneIDi13723
KEGGimmu:13723
UCSCiuc007rym.2 mouse

Similar proteinsi

Entry informationi

Entry nameiEMB_MOUSE
AccessioniPrimary (citable) accession number: P21995
Secondary accession number(s): Q3UFF1
, Q8C2J8, Q8C543, Q96C38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 2005
Last modified: March 28, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health