P21994 (CXB2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gap junction beta-2 protein

Alternative name(s):
Connexin-26
Short name=Cx26

Gene names

Name:	Gjb2
Synonyms:	Cxn-26

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subunit structure	A connexon is composed of a hexamer of connexins. Interacts with CNST By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Tissue specificity	Liver, kidney, intestine, lung, spleen, stomach, testis and brain, but not heart and adult skeletal muscle.
Sequence similarities	Belongs to the connexin family. Beta-type (group I) subfamily.

Ontologies

Keywords
Biological process	Hearing
Cellular component	Cell junction Cell membrane Gap junction Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell-cell signaling Traceable author statement. Source: RGD male genitalia development Inferred from expression pattern. Source: RGD sensory perception of sound Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	connexon complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lateral plasma membrane Inferred from direct assay. Source: RGD
Molecular function	gap junction channel activity Traceable author statement. Source: RGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cnst	Q8CBC4-3	2	EBI-2616119,EBI-2615407	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	Gap junction beta-2 protein		PRO_0000057860
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 40	20	Helical; Potential
	Topological domain	41 – 75	35	Extracellular Potential
	Transmembrane	76 – 98	23	Helical; Potential
	Topological domain	99 – 139	41	Cytoplasmic Potential
	Transmembrane	140 – 162	23	Helical; Potential
	Topological domain	163 – 192	30	Extracellular Potential
	Transmembrane	193 – 215	23	Helical; Potential
	Topological domain	216 – 226	11	Cytoplasmic Potential
Amino acid modifications
	Disulfide bond	53 ↔ 180		By similarity
	Disulfide bond	60 ↔ 174		By similarity
	Disulfide bond	64 ↔ 169		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21994 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: E36EC95F1B235A0D	FASTA	226	26,451
	10 20 30 40 50 60 MDWGTLQSIL GGVNKHSTSI GKIWLTVLFI FRIMILVVAA KEVWGDEQAD FVCNTLQPGC 70 80 90 100 110 120 KNVCYDHYFP ISHIRLWALQ LIMVSTPALL VAMHVAYRRH EKKRKFMKGE IKNEFKDIEE 130 140 150 160 170 180 IKTQKVRIEG SLWWTYTTSI FFRVIFEAVF MYVFYIMYNG FFMQRLVKCN AWPCPNTVDC 190 200 210 220 FISRPTEKTV FTVFMISVSG ICILLNITEL CYLFIRYCSG KSKRPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA." Zhang J.T., Nicholson B.J. J. Cell Biol. 109:3391-3401(1989) [PubMed: 2557354] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Two homologous protein components of hepatic gap junctions." Nicholson B.J., Dermietzel R., Teplow D., Traub O., Willecke K., Revel J.-P. Nature 329:732-734(1987) [PubMed: 2823143] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-18.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC078952 mRNA. Translation: AAH78952.1.
IPI	IPI00208534.
PIR	A33646.
RefSeq	NP_001004099.1. NM_001004099.1.
UniGene	Rn.219269.
3D structure databases
ProteinModelPortal	P21994.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48901N.
IntAct	P21994. 1 interaction.
STRING	P21994.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000011711; ENSRNOP00000011711; ENSRNOG00000008855.
GeneID	394266.
KEGG	rno:394266.
UCSC	NM_001004099. rat.
Organism-specific databases
CTD	2706.
RGD	728891. Gjb2.
Phylogenomic databases
eggNOG	maNOG08829.
HOVERGEN	HBG009576.
InParanoid	P21994.
OMA	MYVFYIM.
OrthoDB	EOG4PK28F.
PhylomeDB	P21994.
Gene expression databases
ArrayExpress	P21994.
Genevestigator	P21994.
GermOnline	ENSRNOG00000008855. Rattus norvegicus.
Family and domain databases
InterPro	IPR000500. Connexin. IPR002268. Connexin26. IPR019570. Connexin_CCC. IPR017990. Connexin_CS. IPR013092. Connexin_N. [Graphical view]
KO	K07621.
PANTHER	PTHR11984. Connexin. 1 hit. PTHR11984:SF24. Connexin26. 1 hit.
Pfam	PF00029. Connexin. 1 hit. PF10582. Connexin_CCC. 1 hit. [Graphical view]
PRINTS	PR00206. CONNEXIN. PR01139. CONNEXINB2.
SMART	SM00037. CNX. 1 hit. SM01089. Connexin_CCC. 1 hit. [Graphical view]
PROSITE	PS00407. CONNEXINS_1. 1 hit. PS00408. CONNEXINS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	692713.

Entry information

Entry name

CXB2_RAT

Accession

Primary (citable) accession number: P21994

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program