P21979 (SPAA_STRDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell surface antigen I/II

Cleaved into the following chain:

1. Cell surface antigen II

Gene names

Name:

spaA

Organism

Streptococcus downei (Streptococcus sobrinus)

Taxonomic identifier

1317 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	1528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Subcellular location	Secreted › cell wall; Peptidoglycan-anchor Potential.
Miscellaneous	Immunodominant determinants are located in the C-terminal two-thirds of the SpaA protein.
Sequence similarities	Belongs to the SpaP/Ssp5/SpaA family.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Repeat Signal
PTM	Peptidoglycan-anchor
Gene Ontology (GO)
Cellular_component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 50	50	Potential
	Chain	51 – ?		Cell surface antigen I/II		PRO_0000005666
	Chain	? – 1506		Cell surface antigen II		PRO_0000005667
	Propeptide	1507 – 1528	22	Removed by sortase Potential		PRO_0000005668
Regions
	Motif	1503 – 1507	5	LPXTG sorting signal Potential
Amino acid modifications
	Modified residue	1506	1	Pentaglycyl murein peptidoglycan amidated threonine Potential
Experimental info
	Sequence conflict	427	1	A → E in AAA26977. Ref.2
	Sequence conflict	431	1	Q → K in AAA26977. Ref.2
	Sequence conflict	434	1	Q → K in AAA26977. Ref.2
	Sequence conflict	531	1	A → S in AAA26977. Ref.2
	Sequence conflict	600	1	A → S in AAA26977. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P21979 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: B235F9CCD92838E0	FASTA	1,528	165,573
	10 20 30 40 50 60 MLQKCKLEGI IICNEKRLLG AAKVKSGRTL SGALLGTAIL ASGAGQKALA EETSTTSTSG 70 80 90 100 110 120 GDTAVVGTET GNPATNLPDK QDNPSSQAET SQAQARQKTG AMSVDVSTSE LDEAAKSPQE 130 140 150 160 170 180 AGVTVSQDAT VNKGTVEPSD EANQKEPEIK DDYSKQAADI QKATEDYKAS VAANQAETDR 190 200 210 220 230 240 INQEIAAKKA QYEQDLAANK AEVERSLMRM RKPRPIYEAK LAQNQKDLAA IQQANSDSQA 250 260 270 280 290 300 AYAAAKEAYD KEWARVQAAN AAAKKAYEEA LAANTAKNDQ IKAEIEAIQQ RSAKADYEAK 310 320 330 340 350 360 LAQYEKDLAA AQAGNAANEA DYQAKKAAYE QELARVQAAN AAAKQAYEQA LAANSAKNAQ 370 380 390 400 410 420 ITAENEAIQQ NAQAKADYEA KLAQYQKDLA AAQSGNAANE ADYQEKLAAY EKELARVQAA 430 440 450 460 470 480 NAAAKQAYEQ QVQQANAKNA EITEANRAIR ERNAKAKTDY ELKLSKYQEE LAQYKKDLAE 490 500 510 520 530 540 YPAKLQAYQD EQAAIKAALA ELEKHKNEDG NLSEPSAQSL VYDLEPNAQV ALVTDGKLLK 550 560 570 580 590 600 ASALDEAFSH DEKNYNNHLL QPDNLNVTYL EQADDVASSV ELFGNFGDKA GWTTTVSNGA 610 620 630 640 650 660 EVKFASVLLK RGQSATATYT NLKNSYYNGK KISKVVYKYT VDPDSKFQNP TGNVWLGIFT 670 680 690 700 710 720 DPTLGVFASA YTGQNEKDTS IFIKNEFTFY DEDGNPIDFD NALLSVASLN REHNSIEMAK 730 740 750 760 770 780 DYSGTFVKIS GSSIGEKNGM IYATDTLNFK KGEGGSLHTM YTRASEPGSG WDSADAPNSW 790 800 810 820 830 840 YGAGAVRMSG PNNYITLGAT SATNVLSLAE MPQVPGKDNT AGKKPNIWYS LNGKIRAVNV 850 860 870 880 890 900 PKVTKEKPTP PVEPTKPDEP TYEVEKELVD LPVEPKYEPE PTPPSKNPDQ SIPEKPVEPT 910 920 930 940 950 960 YEVEKELEPA PVEPSYEKEP TPPQSTPDQE EPEKPVEPSY QSLPTPPVEP VYETVPGPVS 970 980 990 1000 1010 1020 VPTVRYHYYK LAVQPGVTKE IKNQDDLDID KTLVAKQSTV KFQLKTADLP AGRPETTSFV 1030 1040 1050 1060 1070 1080 LMDPLPSGYQ LNLEATKVAS PGFEASYDAM THTVTFTATA ETLAALNQDL TKAVATIYPT 1090 1100 1110 1120 1130 1140 VVGQVLNDGA TYTNNFTLMV NDAYGIKSNI VRVTTPGKPN DPDNPSNNYI TPHKVNKNEN 1150 1160 1170 1180 1190 1200 GVVIDGKSVL AGTTNYYELT WDLDQYKGDK SAKEIIQKGF FYVDDYPEEA LDLRTDLIKL 1210 1220 1230 1240 1250 1260 TDANGKAVTG VSVADYASLE AAPAAVQDML KKANIIPKGA FQVFTADDPQ AFYDAYVVTG 1270 1280 1290 1300 1310 1320 TDLTIVTPMT VKAEMGKTGG SYENRAYQID FGNGYESNLV VNNVPKINPE KDVTLTMDPA 1330 1340 1350 1360 1370 1380 DSTNVDGQTI ALNQVFNYRL IGGIIPADHA EELFEYSFSD DYDQTGDQYT GQYKAFAKVD 1390 1400 1410 1420 1430 1440 LTLKDGTIIK AGTDLTSYTE AQVDEANGQI VVTFKEDFLR SVSVDSAFQA EVYLQMKRIA 1450 1460 1470 1480 1490 1500 VGTFANTYVN TVNGITYSSN TVRTSTPEPK QPSPVDPKTT TTVVFQPRQG KAYQPAPPAG 1510 1520 AQLPATGDSS NAYLPLLGLV SLTAGFSC

References

[1]	"Sequence and structural analysis of surface protein antigen I/II (SpaA) of Streptococcus sobrinus." Lapolla R.J., Haron J.A., Kelly C.G., Taylor W.R., Bohart C., Hendricks M., Pyati J., Graff R.T., Ma J.K.-C., Lehner T. Infect. Immun. 59:2677-2685(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 6715 / Serotype G.
[2]	"Regions of the Streptococcus sobrinus spaA gene encoding major determinants of antigen I." Goldschmidt R.M., Thoren-Gordon M., Curtiss R. III J. Bacteriol. 172:3988-4001(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 423-817.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57841 Genomic DNA. Translation: CAA40973.1. M38210 Genomic DNA. Translation: AAA26977.1.
3D structure databases
ProteinModelPortal	P21979.
SMR	P21979. Positions 475-852.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR026345. Adh_isopep-form_adh_dom. IPR013574. Glucan-bd_C/Surface_Ag-I/II_V. IPR019931. LPXTG-motif_cell_wall_anchor. IPR009578. Surface_Ag_Streptococcal. [Graphical view]
Pfam	PF08363. GbpC. 1 hit. PF06696. Strep_SA_rep. 5 hits. [Graphical view]
SUPFAM	SSF74914. Glucan-bd_C/Surface_Ag-I/II_V. 1 hit.
TIGRFAMs	TIGR04228. isopep_sspB_C2. 1 hit. TIGR01167. LPXTG_anchor. 1 hit.
PROSITE	PS50847. GRAM_POS_ANCHORING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAA_STRDO

Accession

Primary (citable) accession number: P21979

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program