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P21965

- MCK1_YEAST

UniProt

P21965 - MCK1_YEAST

Protein

Protein kinase MCK1

Gene

MCK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 148 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    May be an autophosphorylating tyrosine kinase, a bifunctional (serine/tyrosine-specific) protein kinase, or a serine kinase that is a substrate for an associated tyrosine kinase. MCK1 is a transcriptional activator of IME1, it stimulates spore maturation, and play a positive regulatory role in both mitotic centromere function and activation of early meiotic gene expression.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei68 – 681ATPPROSITE-ProRule annotation
    Active sitei164 – 1641Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi41 – 499ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin binding Source: SGD
    3. enzyme inhibitor activity Source: SGD
    4. protein binding Source: IntAct
    5. protein serine/threonine/tyrosine kinase activity Source: SGD
    6. protein serine/threonine kinase activity Source: SGD

    GO - Biological processi

    1. ascospore formation Source: SGD
    2. cellular response to heat Source: SGD
    3. cellular response to salt stress Source: SGD
    4. double-strand break repair via nonhomologous end joining Source: SGD
    5. meiotic nuclear division Source: SGD
    6. mitotic sister chromatid segregation Source: SGD
    7. negative regulation of catalytic activity Source: GOC
    8. peptidyl-serine phosphorylation Source: SGD
    9. protein phosphorylation Source: SGD
    10. protein phosphorylation involved in cellular protein catabolic process Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33294-MONOMER.
    BRENDAi2.7.11.26. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein kinase MCK1 (EC:2.7.12.1)
    Alternative name(s):
    Meiosis and centromere regulatory kinase
    Gene namesi
    Name:MCK1
    Synonyms:YPK1
    Ordered Locus Names:YNL307C
    ORF Names:N0392
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL307c.
    SGDiS000005251. MCK1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 375374Protein kinase MCK1PRO_0000086317Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei198 – 1981Phosphoserine2 Publications
    Modified residuei199 – 1991Phosphotyrosine2 Publications
    Modified residuei202 – 2021Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated at tyrosine and serine.3 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP21965.
    PaxDbiP21965.
    PeptideAtlasiP21965.

    Expressioni

    Gene expression databases

    GenevestigatoriP21965.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC14Q006842EBI-10517,EBI-4192

    Protein-protein interaction databases

    BioGridi35532. 426 interactions.
    DIPiDIP-5860N.
    IntActiP21965. 43 interactions.
    MINTiMINT-681308.
    STRINGi4932.YNL307C.

    Structurei

    3D structure databases

    ProteinModelPortaliP21965.
    SMRiP21965. Positions 41-366.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 327293Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000116084.
    HOGENOMiHOG000233017.
    KOiK00924.
    OMAiYKSRELE.
    OrthoDBiEOG7CZKGN.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21965-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTEEQNGVP LQRGSEFIAD DVTSNKSNNT RRMLVKEYRK IGRGAFGTVV    50
    QAYLTQDKKN WLGPFAIKKV PAHTEYKSRE LQILRIADHP NIVKLQYFFT 100
    HLSPQDNKVY QHLAMECLPE TLQIEINRYV TNKLEMPLKH IRLYTYQIAR 150
    GMLYLHGLGV CHRDIKPSNV LVDPETGVLK ICDFGSAKKL EHNQPSISYI 200
    CSRFYRAPEL IIGCTQYTTQ IDIWGLGCVM GEMLIGKAIF QGQEPLLQLR 250
    EIAKLLGPPD KRFIFFSNPA YDGPLFSKPL FSGSSQQRFE KYFGHSGPDG 300
    IDLLMKILVY EPQQRLSPRR ILAHQFFNEL RNDDTFLPRG FTEPIKLPNL 350
    FDFNDFELQI LGEFADKIKP TKVAE 375
    Length:375
    Mass (Da):43,136
    Last modified:August 1, 1991 - v1
    Checksum:iBCF0C776B6E3841B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55054 Genomic DNA. Translation: CAA38895.1.
    M55984 Genomic DNA. Translation: AAA34764.1.
    Z46259 Genomic DNA. Translation: CAA86388.1.
    Z71583 Genomic DNA. Translation: CAA96236.1.
    Z71582 Genomic DNA. Translation: CAA96235.1.
    BK006947 Genomic DNA. Translation: DAA10254.1.
    PIRiA39622.
    RefSeqiNP_014092.1. NM_001183145.1.

    Genome annotation databases

    EnsemblFungiiYNL307C; YNL307C; YNL307C.
    GeneIDi855409.
    KEGGisce:YNL307C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55054 Genomic DNA. Translation: CAA38895.1 .
    M55984 Genomic DNA. Translation: AAA34764.1 .
    Z46259 Genomic DNA. Translation: CAA86388.1 .
    Z71583 Genomic DNA. Translation: CAA96236.1 .
    Z71582 Genomic DNA. Translation: CAA96235.1 .
    BK006947 Genomic DNA. Translation: DAA10254.1 .
    PIRi A39622.
    RefSeqi NP_014092.1. NM_001183145.1.

    3D structure databases

    ProteinModelPortali P21965.
    SMRi P21965. Positions 41-366.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35532. 426 interactions.
    DIPi DIP-5860N.
    IntActi P21965. 43 interactions.
    MINTi MINT-681308.
    STRINGi 4932.YNL307C.

    Proteomic databases

    MaxQBi P21965.
    PaxDbi P21965.
    PeptideAtlasi P21965.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL307C ; YNL307C ; YNL307C .
    GeneIDi 855409.
    KEGGi sce:YNL307C.

    Organism-specific databases

    CYGDi YNL307c.
    SGDi S000005251. MCK1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000116084.
    HOGENOMi HOG000233017.
    KOi K00924.
    OMAi YKSRELE.
    OrthoDBi EOG7CZKGN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33294-MONOMER.
    BRENDAi 2.7.11.26. 984.

    Miscellaneous databases

    NextBioi 979250.
    PROi P21965.

    Gene expression databases

    Genevestigatori P21965.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity."
      Dailey D., Schieven G.L., Lim M.-Y., Marquardt H., Gilmore T., Thorner J., Martin G.S.
      Mol. Cell. Biol. 10:6244-6256(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. "The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression."
      Neigeborn L., Mitchell A.P.
      Genes Dev. 5:533-548(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "A suppressor of a centromere DNA mutation encodes a putative protein kinase (MCK1)."
      Shero J.H., Hieter P.
      Genes Dev. 5:549-560(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames."
      Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
      Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. "Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene."
      Maurer K.C.T., Urbanus J.H.M., Planta R.J.
      Yeast 11:1303-1310(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
      Strain: ATCC 96604 / S288c / FY1679.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198; TYR-199 AND SER-202, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMCK1_YEAST
    AccessioniPrimary (citable) accession number: P21965
    Secondary accession number(s): D6W0N8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 148 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 396 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3