P21965 (MCK1_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 135.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Protein kinase MCK1 EC=2.7.11.1 Alternative name(s): Meiosis and centromere regulatory kinase | ||||||||
| Gene names |
| ||||||||
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › ![]() |
Protein attributes
| Sequence length | 375 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | May be an autophosphorylating tyrosine kinase, a bifunctional (serine/tyrosine-specific) protein kinase, or a serine kinase that is a substrate for an associated tyrosine kinase. MCK1 is a transcriptional activator of IME1, it stimulates spore maturation, and play a positive regulatory role in both mitotic centromere function and activation of early meiotic gene expression. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Post-translational modification | Phosphorylated at tyrosine and serine. |
| Miscellaneous | Present with 396 molecules/cell in log phase SD medium. |
| Sequence similarities | Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | ascospore formation Inferred from mutant phenotype Ref.2. Source: SGD double-strand break repair via nonhomologous end joiningInferred from mutant phenotype PubMed 12399380. Source: SGD meiosisInferred from mutant phenotype Ref.2. Source: SGD mitotic sister chromatid segregationInferred from genetic interaction Ref.3. Source: SGD |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activityInferred from sequence or structural similarity PubMed 12529445. Source: SGD protein serine/threonine/tyrosine kinase activityInferred from direct assay PubMed 8407952. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| CDC14 | Q00684 | 2 | EBI-10517,EBI-4192 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 375 | 375 | Protein kinase MCK1 | PRO_0000086317 | |||||
Regions | |||||||||
| Domain | 35 – 327 | 293 | Protein kinase | ||||||
| Nucleotide binding | 41 – 49 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 164 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 68 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 24 | 1 | Phosphoserine Ref.11 Ref.12 | ||||||
| Modified residue | 27 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 48 | 1 | Phosphothreonine Ref.11 | ||||||
| Modified residue | 196 | 1 | Phosphoserine Ref.12 | ||||||
| Modified residue | 198 | 1 | Phosphoserine Ref.11 Ref.12 | ||||||
| Modified residue | 199 | 1 | Phosphotyrosine Ref.9 Ref.10 Ref.12 | ||||||
| Modified residue | 202 | 1 | Phosphoserine Ref.12 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity." Dailey D., Schieven G.L., Lim M.-Y., Marquardt H., Gilmore T., Thorner J., Martin G.S. Mol. Cell. Biol. 10:6244-6256(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. |
| [2] | "The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression." Neigeborn L., Mitchell A.P. Genes Dev. 5:533-548(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "A suppressor of a centromere DNA mutation encodes a putative protein kinase (MCK1)." Shero J.H., Hieter P. Genes Dev. 5:549-560(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676. |
| [5] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J.Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [6] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [7] | "Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16. Strain: ATCC 96604 / S288c / FY1679. |
| [8] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [9] | "A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, MASS SPECTROMETRY. Strain: SUB592. |
| [10] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, MASS SPECTROMETRY. Strain: ADR376. |
| [11] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; THR-48 AND SER-198, MASS SPECTROMETRY. |
| [12] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; SER-27; SER-196; SER-198; TYR-199 AND SER-202, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X55054 Genomic DNA. Translation: CAA38895.1. M55984 Genomic DNA. Translation: AAA34764.1. Z46259 Genomic DNA. Translation: CAA86388.1. Z71583 Genomic DNA. Translation: CAA96236.1. Z71582 Genomic DNA. Translation: CAA96235.1. BK006947 Genomic DNA. Translation: DAA10254.1. |
| PIR | A39622. |
| RefSeq | NP_014092.1. NM_001183145.1. |
3D structure databases | |
| ProteinModelPortal | P21965. |
| SMR | P21965. Positions 11-375. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-5860N. |
| IntAct | P21965. 44 interactions. |
| MINT | MINT-681308. |
| STRING | 4932.YNL307C. |
Proteomic databases | |
| PaxDb | P21965. |
| PeptideAtlas | P21965. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YNL307C; YNL307C; YNL307C. |
| GeneID | 855409. |
| KEGG | sce:YNL307C. |
Organism-specific databases | |
| CYGD | YNL307c. |
| SGD | S000005251. MCK1. |
Phylogenomic databases | |
| eggNOG | COG0515. |
| GeneTree | ENSGT00600000085096. |
| HOGENOM | HOG000233017. |
| KO | K00924. |
| OMA | YKSRELE. |
| OrthoDB | EOG4MGWH1. |
Enzyme and pathway databases | |
| BioCyc | YEAST:G3O-33294-MONOMER. |
| BRENDA | 2.7.11.26. 984. |
Gene expression databases | |
| Genevestigator | P21965. |
| GermOnline | YNL307C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| SUPFAM | SSF56112. Kinase_like. 1 hit. |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 979250. |
Entry information
| Entry name | MCK1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P21965 Secondary accession number(s): D6W0N8 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XIV Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with
