Protein kinase MCK1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P21965 (MCK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 135. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein kinase MCK1
EC=2.7.11.1

Alternative name(s):
Meiosis and centromere regulatory kinase

Gene names

Name:	MCK1
Synonyms:	YPK1
Ordered Locus Names:	YNL307C
ORF Names:	N0392

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be an autophosphorylating tyrosine kinase, a bifunctional (serine/tyrosine-specific) protein kinase, or a serine kinase that is a substrate for an associated tyrosine kinase. MCK1 is a transcriptional activator of IME1, it stimulates spore maturation, and play a positive regulatory role in both mitotic centromere function and activation of early meiotic gene expression.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Post-translational modification	Phosphorylated at tyrosine and serine.
Miscellaneous	Present with 396 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ascospore formation Inferred from mutant phenotype Ref.2. Source: SGD double-strand break repair via nonhomologous end joining Inferred from mutant phenotype PubMed 12399380. Source: SGD meiosis Inferred from mutant phenotype Ref.2. Source: SGD mitotic sister chromatid segregation Inferred from genetic interaction Ref.3. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from sequence or structural similarity PubMed 12529445. Source: SGD protein serine/threonine/tyrosine kinase activity Inferred from direct assay PubMed 8407952. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC14	Q00684	2	EBI-10517,EBI-4192

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 375

375

Protein kinase MCK1

PRO_0000086317

Regions

Domain

35 – 327

293

Protein kinase

Nucleotide binding

41 – 49

ATP By similarity

Sites

Active site

164

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.11 Ref.12

Modified residue

Phosphoserine Ref.12

Modified residue

Phosphothreonine Ref.11

Modified residue

196

Phosphoserine Ref.12

Modified residue

198

Phosphoserine Ref.11 Ref.12

Modified residue

199

Phosphotyrosine Ref.9 Ref.10 Ref.12

Modified residue

202

Phosphoserine Ref.12

Sequences

Sequence

Length

Mass (Da)

Tools

P21965 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: BCF0C776B6E3841B
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FASTA

375

43,136

        10         20         30         40         50         60 
MSTEEQNGVP LQRGSEFIAD DVTSNKSNNT RRMLVKEYRK IGRGAFGTVV QAYLTQDKKN 

        70         80         90        100        110        120 
WLGPFAIKKV PAHTEYKSRE LQILRIADHP NIVKLQYFFT HLSPQDNKVY QHLAMECLPE 

       130        140        150        160        170        180 
TLQIEINRYV TNKLEMPLKH IRLYTYQIAR GMLYLHGLGV CHRDIKPSNV LVDPETGVLK 

       190        200        210        220        230        240 
ICDFGSAKKL EHNQPSISYI CSRFYRAPEL IIGCTQYTTQ IDIWGLGCVM GEMLIGKAIF 

       250        260        270        280        290        300 
QGQEPLLQLR EIAKLLGPPD KRFIFFSNPA YDGPLFSKPL FSGSSQQRFE KYFGHSGPDG 

       310        320        330        340        350        360 
IDLLMKILVY EPQQRLSPRR ILAHQFFNEL RNDDTFLPRG FTEPIKLPNL FDFNDFELQI 

       370 
LGEFADKIKP TKVAE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity." Dailey D., Schieven G.L., Lim M.-Y., Marquardt H., Gilmore T., Thorner J., Martin G.S. Mol. Cell. Biol. 10:6244-6256(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression." Neigeborn L., Mitchell A.P. Genes Dev. 5:533-548(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A suppressor of a centromere DNA mutation encodes a putative protein kinase (MCK1)." Shero J.H., Hieter P. Genes Dev. 5:549-560(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16. Strain: ATCC 96604 / S288c / FY1679.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, MASS SPECTROMETRY. Strain: SUB592.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; THR-48 AND SER-198, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; SER-27; SER-196; SER-198; TYR-199 AND SER-202, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55054 Genomic DNA. Translation: CAA38895.1. M55984 Genomic DNA. Translation: AAA34764.1. Z46259 Genomic DNA. Translation: CAA86388.1. Z71583 Genomic DNA. Translation: CAA96236.1. Z71582 Genomic DNA. Translation: CAA96235.1. BK006947 Genomic DNA. Translation: DAA10254.1.
PIR	A39622.
RefSeq	NP_014092.1. NM_001183145.1.
3D structure databases
ProteinModelPortal	P21965.
SMR	P21965. Positions 11-375.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5860N.
IntAct	P21965. 44 interactions.
MINT	MINT-681308.
STRING	4932.YNL307C.
Proteomic databases
PaxDb	P21965.
PeptideAtlas	P21965.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL307C; YNL307C; YNL307C.
GeneID	855409.
KEGG	sce:YNL307C.
Organism-specific databases
CYGD	YNL307c.
SGD	S000005251. MCK1.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00600000085096.
HOGENOM	HOG000233017.
KO	K00924.
OMA	YKSRELE.
OrthoDB	EOG4MGWH1.
Enzyme and pathway databases
BioCyc	YEAST:G3O-33294-MONOMER.
BRENDA	2.7.11.26. 984.
Gene expression databases
Genevestigator	P21965.
GermOnline	YNL307C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	979250.

Entry information

Entry name

MCK1_YEAST

Accession

Primary (citable) accession number: P21965
Secondary accession number(s): D6W0N8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	May 29, 2013
This is version 135 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents