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Protein

Protein kinase MCK1

Gene

MCK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be an autophosphorylating tyrosine kinase, a bifunctional (serine/tyrosine-specific) protein kinase, or a serine kinase that is a substrate for an associated tyrosine kinase. MCK1 is a transcriptional activator of IME1, it stimulates spore maturation, and play a positive regulatory role in both mitotic centromere function and activation of early meiotic gene expression.

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68ATPPROSITE-ProRule annotation1
Active sitei164Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi41 – 49ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin binding Source: SGD
  • enzyme inhibitor activity Source: SGD
  • protein serine/threonine/tyrosine kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • ascospore formation Source: SGD
  • cellular response to heat Source: SGD
  • cellular response to salt stress Source: SGD
  • double-strand break repair via nonhomologous end joining Source: SGD
  • meiotic cell cycle Source: SGD
  • mitotic sister chromatid segregation Source: SGD
  • peptidyl-serine phosphorylation Source: SGD
  • protein phosphorylation Source: SGD
  • protein phosphorylation involved in cellular protein catabolic process Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33294-MONOMER.
BRENDAi2.7.11.26. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase MCK1 (EC:2.7.12.1)
Alternative name(s):
Meiosis and centromere regulatory kinase
Gene namesi
Name:MCK1
Synonyms:YPK1
Ordered Locus Names:YNL307C
ORF Names:N0392
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL307C.
SGDiS000005251. MCK1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000863172 – 375Protein kinase MCK1Add BLAST374

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei199PhosphotyrosineCombined sources1
Modified residuei202PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated at tyrosine and serine.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP21965.
PRIDEiP21965.

PTM databases

iPTMnetiP21965.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006842EBI-10517,EBI-4192

GO - Molecular functioni

  • cyclin binding Source: SGD

Protein-protein interaction databases

BioGridi35532. 586 interactors.
DIPiDIP-5860N.
IntActiP21965. 44 interactors.
MINTiMINT-681308.
STRINGi4932.YNL307C.

Structurei

3D structure databases

ProteinModelPortaliP21965.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 327Protein kinasePROSITE-ProRule annotationAdd BLAST293

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00840000131002.
HOGENOMiHOG000233017.
InParanoidiP21965.
KOiK00924.
OMAiPAQTEYK.
OrthoDBiEOG092C1O26.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTEEQNGVP LQRGSEFIAD DVTSNKSNNT RRMLVKEYRK IGRGAFGTVV
60 70 80 90 100
QAYLTQDKKN WLGPFAIKKV PAHTEYKSRE LQILRIADHP NIVKLQYFFT
110 120 130 140 150
HLSPQDNKVY QHLAMECLPE TLQIEINRYV TNKLEMPLKH IRLYTYQIAR
160 170 180 190 200
GMLYLHGLGV CHRDIKPSNV LVDPETGVLK ICDFGSAKKL EHNQPSISYI
210 220 230 240 250
CSRFYRAPEL IIGCTQYTTQ IDIWGLGCVM GEMLIGKAIF QGQEPLLQLR
260 270 280 290 300
EIAKLLGPPD KRFIFFSNPA YDGPLFSKPL FSGSSQQRFE KYFGHSGPDG
310 320 330 340 350
IDLLMKILVY EPQQRLSPRR ILAHQFFNEL RNDDTFLPRG FTEPIKLPNL
360 370
FDFNDFELQI LGEFADKIKP TKVAE
Length:375
Mass (Da):43,136
Last modified:August 1, 1991 - v1
Checksum:iBCF0C776B6E3841B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55054 Genomic DNA. Translation: CAA38895.1.
M55984 Genomic DNA. Translation: AAA34764.1.
Z46259 Genomic DNA. Translation: CAA86388.1.
Z71583 Genomic DNA. Translation: CAA96236.1.
Z71582 Genomic DNA. Translation: CAA96235.1.
BK006947 Genomic DNA. Translation: DAA10254.1.
PIRiA39622.
RefSeqiNP_014092.1. NM_001183145.1.

Genome annotation databases

EnsemblFungiiYNL307C; YNL307C; YNL307C.
GeneIDi855409.
KEGGisce:YNL307C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMCK1_YEAST
AccessioniPrimary (citable) accession number: P21965
Secondary accession number(s): D6W0N8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 5, 2017
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names