Reviewed, UniProtKB/Swiss-Prot P21965 (MCK1_YEAST)

Names and origin

Protein names

Recommended name:
    Protein kinase MCK1
    EC=2.7.11.1
Alternative name(s):
    Meiosis and centromere regulatory kinase

Gene names

Name:	MCK1
Synonyms:	YPK1
Ordered Locus Names:	YNL307C
ORF Names:	N0392

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be an autophosphorylating tyrosine kinase, a bifunctional (serine/tyrosine-specific) protein kinase, or a serine kinase that is a substrate for an associated tyrosine kinase. MCK1 is a transcriptional activator of IME1, it stimulates spore maturation, and play a positive regulatory role in both mitotic centromere function and activation of early meiotic gene expression.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Post-translational modification	Phosphorylated at tyrosine and serine. Ref.8 Ref.9 Ref.10 Ref.11
Miscellaneous	Present with 396 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ascospore formation Ref.2 Inferred from mutant phenotype. Source: SGD double-strand break repair via nonhomologous end joining Inferred from mutant phenotype. Source: SGD meiosis Ref.2 Inferred from mutant phenotype. Source: SGD mitotic sister chromatid segregation Ref.3 Inferred from genetic interaction. Source: SGD protein amino acid phosphorylation Inferred from direct assay. Source: SGD
Cellular component	soluble fraction Ref.1 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine/tyrosine kinase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PEX11	Q12462	1	EBI-10517,EBI-13198

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Protein kinase MCK1		PRO_0000086317
Regions
	Domain	35 – 327	293	Protein kinase
	Nucleotide binding	41 – 49	9	ATP By similarity
Sites
	Active site	164	1	Proton acceptor By similarity
	Binding site	68	1	ATP By similarity
Amino acid modifications
	Modified residue	24	1	Phosphoserine Ref.10 Ref.11
	Modified residue	27	1	Phosphoserine Ref.11
	Modified residue	48	1	Phosphothreonine Ref.10
	Modified residue	196	1	Phosphoserine Ref.11
	Modified residue	198	1	Phosphoserine Ref.10 Ref.11
	Modified residue	199	1	Phosphotyrosine Ref.8 Ref.9 Ref.11
	Modified residue	202	1	Phosphoserine Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P21965-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: BCF0C776B6E3841B	FASTA	375	43,136
	10 20 30 40 50 60 MSTEEQNGVP LQRGSEFIAD DVTSNKSNNT RRMLVKEYRK IGRGAFGTVV QAYLTQDKKN 70 80 90 100 110 120 WLGPFAIKKV PAHTEYKSRE LQILRIADHP NIVKLQYFFT HLSPQDNKVY QHLAMECLPE 130 140 150 160 170 180 TLQIEINRYV TNKLEMPLKH IRLYTYQIAR GMLYLHGLGV CHRDIKPSNV LVDPETGVLK 190 200 210 220 230 240 ICDFGSAKKL EHNQPSISYI CSRFYRAPEL IIGCTQYTTQ IDIWGLGCVM GEMLIGKAIF 250 260 270 280 290 300 QGQEPLLQLR EIAKLLGPPD KRFIFFSNPA YDGPLFSKPL FSGSSQQRFE KYFGHSGPDG 310 320 330 340 350 360 IDLLMKILVY EPQQRLSPRR ILAHQFFNEL RNDDTFLPRG FTEPIKLPNL FDFNDFELQI 370 LGEFADKIKP TKVAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity." Dailey D., Schieven G.L., Lim M.-Y., Marquardt H., Gilmore T., Thorner J., Martin G.S. Mol. Cell. Biol. 10:6244-6256(1990) [PubMed: 1701015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression." Neigeborn L., Mitchell A.P. Genes Dev. 5:533-548(1991) [PubMed: 2010083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A suppressor of a centromere DNA mutation encodes a putative protein kinase (MCK1)." Shero J.H., Hieter P. Genes Dev. 5:549-560(1991) [PubMed: 2010084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames." Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C. Yeast 11:1077-1085(1995) [PubMed: 7502583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / FY1676.
[5]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[6]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16. Strain: ATCC 96604 / S288c / FY1679.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, MASS SPECTROMETRY.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; THR-48 AND SER-198, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; SER-27; SER-196; SER-198; TYR-199 AND SER-202, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55054 Genomic DNA. Translation: CAA38895.1. M55984 Genomic DNA. Translation: AAA34764.1. Z46259 Genomic DNA. Translation: CAA86388.1. Z71583 Genomic DNA. Translation: CAA96236.1. Z71582 Genomic DNA. Translation: CAA96235.1.
PIR	A39622.
RefSeq	NP_014092.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5860N.
IntAct	P21965. 11 interactions.
STRING	P21965.
Proteomic databases
PeptideAtlas	P21965.
PRIDE	P21965.
Genome annotation databases
Ensembl	YNL307C; YNL307C; YNL307C; Saccharomyces cerevisiae. [Genome view]
GeneID	855409.
KEGG	sce:YNL307C.
NMPDR	fig|4932.3.peg.5154.
Organism-specific databases
CYGD	YNL307c.
SGD	S000005251. MCK1.
Phylogenomic databases
HOGENOM	P21965.
OMA	IDIWGLG
OrthoDB	EOG9S4QZB
Enzyme and pathway databases
BRENDA	2.7.11.1. 250. 2.7.11.26. 250.
Gene expression databases
ArrayExpress	P21965.
Genevestigator	P21965.
GermOnline	YNL307C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	979250.

Entry information

Entry name

MCK1_YEAST

Accession

Primary (citable) accession number: P21965

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	November 24, 2009
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)