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P21965

- MCK1_YEAST

UniProt

P21965 - MCK1_YEAST

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Protein

Protein kinase MCK1

Gene

MCK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be an autophosphorylating tyrosine kinase, a bifunctional (serine/tyrosine-specific) protein kinase, or a serine kinase that is a substrate for an associated tyrosine kinase. MCK1 is a transcriptional activator of IME1, it stimulates spore maturation, and play a positive regulatory role in both mitotic centromere function and activation of early meiotic gene expression.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681ATPPROSITE-ProRule annotation
Active sitei164 – 1641Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi41 – 499ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin binding Source: SGD
  3. enzyme inhibitor activity Source: SGD
  4. protein serine/threonine/tyrosine kinase activity Source: SGD
  5. protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  1. ascospore formation Source: SGD
  2. cellular response to heat Source: SGD
  3. cellular response to salt stress Source: SGD
  4. double-strand break repair via nonhomologous end joining Source: SGD
  5. meiotic nuclear division Source: SGD
  6. mitotic sister chromatid segregation Source: SGD
  7. negative regulation of catalytic activity Source: GOC
  8. peptidyl-serine phosphorylation Source: SGD
  9. protein phosphorylation Source: SGD
  10. protein phosphorylation involved in cellular protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33294-MONOMER.
BRENDAi2.7.11.26. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase MCK1 (EC:2.7.12.1)
Alternative name(s):
Meiosis and centromere regulatory kinase
Gene namesi
Name:MCK1
Synonyms:YPK1
Ordered Locus Names:YNL307C
ORF Names:N0392
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL307c.
SGDiS000005251. MCK1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 375374Protein kinase MCK1PRO_0000086317Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei198 – 1981Phosphoserine2 Publications
Modified residuei199 – 1991Phosphotyrosine2 Publications
Modified residuei202 – 2021Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated at tyrosine and serine.3 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP21965.
PaxDbiP21965.
PeptideAtlasiP21965.

Expressioni

Gene expression databases

GenevestigatoriP21965.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006842EBI-10517,EBI-4192

Protein-protein interaction databases

BioGridi35532. 427 interactions.
DIPiDIP-5860N.
IntActiP21965. 43 interactions.
MINTiMINT-681308.
STRINGi4932.YNL307C.

Structurei

3D structure databases

ProteinModelPortaliP21965.
SMRiP21965. Positions 11-375.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 327293Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00740000116084.
HOGENOMiHOG000233017.
InParanoidiP21965.
KOiK00924.
OMAiYKSRELE.
OrthoDBiEOG7CZKGN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21965-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTEEQNGVP LQRGSEFIAD DVTSNKSNNT RRMLVKEYRK IGRGAFGTVV
60 70 80 90 100
QAYLTQDKKN WLGPFAIKKV PAHTEYKSRE LQILRIADHP NIVKLQYFFT
110 120 130 140 150
HLSPQDNKVY QHLAMECLPE TLQIEINRYV TNKLEMPLKH IRLYTYQIAR
160 170 180 190 200
GMLYLHGLGV CHRDIKPSNV LVDPETGVLK ICDFGSAKKL EHNQPSISYI
210 220 230 240 250
CSRFYRAPEL IIGCTQYTTQ IDIWGLGCVM GEMLIGKAIF QGQEPLLQLR
260 270 280 290 300
EIAKLLGPPD KRFIFFSNPA YDGPLFSKPL FSGSSQQRFE KYFGHSGPDG
310 320 330 340 350
IDLLMKILVY EPQQRLSPRR ILAHQFFNEL RNDDTFLPRG FTEPIKLPNL
360 370
FDFNDFELQI LGEFADKIKP TKVAE
Length:375
Mass (Da):43,136
Last modified:August 1, 1991 - v1
Checksum:iBCF0C776B6E3841B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55054 Genomic DNA. Translation: CAA38895.1.
M55984 Genomic DNA. Translation: AAA34764.1.
Z46259 Genomic DNA. Translation: CAA86388.1.
Z71583 Genomic DNA. Translation: CAA96236.1.
Z71582 Genomic DNA. Translation: CAA96235.1.
BK006947 Genomic DNA. Translation: DAA10254.1.
PIRiA39622.
RefSeqiNP_014092.1. NM_001183145.1.

Genome annotation databases

EnsemblFungiiYNL307C; YNL307C; YNL307C.
GeneIDi855409.
KEGGisce:YNL307C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55054 Genomic DNA. Translation: CAA38895.1 .
M55984 Genomic DNA. Translation: AAA34764.1 .
Z46259 Genomic DNA. Translation: CAA86388.1 .
Z71583 Genomic DNA. Translation: CAA96236.1 .
Z71582 Genomic DNA. Translation: CAA96235.1 .
BK006947 Genomic DNA. Translation: DAA10254.1 .
PIRi A39622.
RefSeqi NP_014092.1. NM_001183145.1.

3D structure databases

ProteinModelPortali P21965.
SMRi P21965. Positions 11-375.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35532. 427 interactions.
DIPi DIP-5860N.
IntActi P21965. 43 interactions.
MINTi MINT-681308.
STRINGi 4932.YNL307C.

Proteomic databases

MaxQBi P21965.
PaxDbi P21965.
PeptideAtlasi P21965.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL307C ; YNL307C ; YNL307C .
GeneIDi 855409.
KEGGi sce:YNL307C.

Organism-specific databases

CYGDi YNL307c.
SGDi S000005251. MCK1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00740000116084.
HOGENOMi HOG000233017.
InParanoidi P21965.
KOi K00924.
OMAi YKSRELE.
OrthoDBi EOG7CZKGN.

Enzyme and pathway databases

BioCyci YEAST:G3O-33294-MONOMER.
BRENDAi 2.7.11.26. 984.

Miscellaneous databases

NextBioi 979250.
PROi P21965.

Gene expression databases

Genevestigatori P21965.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity."
    Dailey D., Schieven G.L., Lim M.-Y., Marquardt H., Gilmore T., Thorner J., Martin G.S.
    Mol. Cell. Biol. 10:6244-6256(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "The yeast MCK1 gene encodes a protein kinase homolog that activates early meiotic gene expression."
    Neigeborn L., Mitchell A.P.
    Genes Dev. 5:533-548(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A suppressor of a centromere DNA mutation encodes a putative protein kinase (MCK1)."
    Shero J.H., Hieter P.
    Genes Dev. 5:549-560(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames."
    Maftahi M., Nicaud J.-M., Levesque H., Gaillardin C.
    Yeast 11:1077-1085(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene."
    Maurer K.C.T., Urbanus J.H.M., Planta R.J.
    Yeast 11:1303-1310(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
    Strain: ATCC 96604 / S288c / FY1679.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-199, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198; TYR-199 AND SER-202, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMCK1_YEAST
AccessioniPrimary (citable) accession number: P21965
Secondary accession number(s): D6W0N8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 29, 2014
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 396 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3