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P21964

- COMT_HUMAN

UniProt

P21964 - COMT_HUMAN

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Protein
Catechol O-methyltransferase
Gene
COMT
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

Cofactori

Binds 1 magnesium ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921S-adenosyl-L-methionine; via amide nitrogen
Binding sitei114 – 1141S-adenosyl-L-methionine By similarity
Binding sitei122 – 1221S-adenosyl-L-methionine
Binding sitei140 – 1401S-adenosyl-L-methionine
Binding sitei141 – 1411S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei169 – 1691S-adenosyl-L-methionine; via amide nitrogen By similarity
Metal bindingi191 – 1911Magnesium
Binding sitei191 – 1911S-adenosyl-L-methionine
Binding sitei194 – 1941Substrate
Metal bindingi219 – 2191Magnesium
Metal bindingi220 – 2201Magnesium
Binding sitei220 – 2201Substrate
Binding sitei249 – 2491Substrate

GO - Molecular functioni

  1. O-methyltransferase activity Source: ProtInc
  2. catechol O-methyltransferase activity Source: UniProtKB-EC
  3. magnesium ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. dopamine catabolic process Source: Ensembl
  2. estrogen metabolic process Source: Ensembl
  3. female pregnancy Source: Ensembl
  4. learning Source: Ensembl
  5. methylation Source: Reactome
  6. multicellular organismal reproductive process Source: Ensembl
  7. negative regulation of dopamine metabolic process Source: Ensembl
  8. negative regulation of smooth muscle cell proliferation Source: Ensembl
  9. neurotransmitter biosynthetic process Source: Reactome
  10. neurotransmitter catabolic process Source: UniProtKB-KW
  11. positive regulation of homocysteine metabolic process Source: Ensembl
  12. response to drug Source: Ensembl
  13. response to lipopolysaccharide Source: Ensembl
  14. response to organic cyclic compound Source: Ensembl
  15. response to pain Source: Ensembl
  16. small molecule metabolic process Source: Reactome
  17. synaptic transmission Source: Reactome
  18. xenobiotic metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine metabolism, Neurotransmitter degradation

Keywords - Ligandi

Magnesium, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:HS01791-MONOMER.
BRENDAi2.1.1.6. 2681.
ReactomeiREACT_15511. Enzymatic degradation of Dopamine by monoamine oxidase.
REACT_15548. Enzymatic degradation of dopamine by COMT.
REACT_6946. Methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Catechol O-methyltransferase (EC:2.1.1.6)
Gene namesi
Name:COMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 22

Organism-specific databases

HGNCiHGNC:2228. COMT.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2620Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. extracellular vesicular exosome Source: UniProt
  3. integral component of membrane Source: UniProtKB-KW
  4. mitochondrion Source: Ensembl
  5. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

MIMi103780. phenotype.
116790. gene+phenotype.
Orphaneti567. 22q11.2 deletion syndrome.
240863. Cisplatin toxicity.
3140. Schizophrenia.
240999. Susceptibility to deafness due to cisplatin treatment.
PharmGKBiPA117.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Catechol O-methyltransferase
PRO_0000020971Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiP21964.
PaxDbiP21964.
PRIDEiP21964.

2D gel databases

REPRODUCTION-2DPAGEIPI00375513.

PTM databases

PhosphoSiteiP21964.

Expressioni

Tissue specificityi

Brain, liver, placenta, lymphocytes and erythrocytes.

Gene expression databases

ArrayExpressiP21964.
BgeeiP21964.
CleanExiHS_COMT.
GenevestigatoriP21964.

Organism-specific databases

HPAiCAB011233.
HPA001169.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
XRN2Q9H0D61EBI-372265,EBI-372110

Protein-protein interaction databases

BioGridi107707. 10 interactions.
IntActiP21964. 6 interactions.
MINTiMINT-4529967.
STRINGi9606.ENSP00000354511.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi55 – 6612
Helixi72 – 8514
Helixi93 – 10715
Beta strandi110 – 1156
Helixi121 – 1277
Beta strandi135 – 1417
Helixi143 – 15513
Helixi159 – 1613
Beta strandi162 – 1676
Helixi169 – 1724
Helixi173 – 1753
Helixi176 – 1805
Beta strandi185 – 1906
Helixi194 – 1963
Helixi197 – 20610
Beta strandi210 – 21910
Helixi221 – 2255
Helixi227 – 2359
Beta strandi239 – 2479
Beta strandi251 – 26212

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A7EX-ray2.80A51-264[»]
3BWMX-ray1.98A52-265[»]
3BWYX-ray1.30A52-265[»]
4PYIX-ray1.35A51-271[»]
4PYJX-ray1.90A51-271[»]
4PYKX-ray2.22A51-271[»]
ProteinModelPortaliP21964.
SMRiP21964. Positions 52-265.

Miscellaneous databases

EvolutionaryTraceiP21964.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni167 – 1704S-adenosyl-L-methionine binding

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4122.
HOGENOMiHOG000046392.
HOVERGENiHBG005376.
InParanoidiP21964.
KOiK00545.
OMAiCTHYSSY.
OrthoDBiEOG7PZRZJ.
PhylomeDBiP21964.
TreeFamiTF329140.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025782. Catechol_O-MeTrfase.
IPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10509. PTHR10509. 1 hit.
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform Membrane-bound (identifier: P21964-1) [UniParc]FASTAAdd to Basket

Also known as: MB-COMT

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPEAPPLLLA AVLLGLVLLV VLLLLLRHWG WGLCLIGWNE FILQPIHNLL    50
MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV 100
DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ 150
RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD 200
TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY 250
REVVDGLEKA IYKGPGSEAG P 271
Length:271
Mass (Da):30,037
Last modified:May 1, 1992 - v2
Checksum:iD2547A1C399AC758
GO
Isoform Soluble (identifier: P21964-2) [UniParc]FASTAAdd to Basket

Also known as: S-COMT

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Show »
Length:221
Mass (Da):24,449
Checksum:iF17399A01E493B63
GO

Mass spectrometryi

Molecular mass is 24352±2 Da from positions 52 - 271. Determined by ESI. 1 Publication

Polymorphismi

Two alleles, COMT*1 or COMT*H with Val-158 and COMT*2 or COMT*L with Met-158 are responsible for a three to four-fold difference in enzymatic activity.
Low enzyme activity alleles are associated with genetic susceptibility to alcoholism [MIMi:103780].

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti34 – 341C → S.2 Publications
Corresponds to variant rs6270 [ dbSNP | Ensembl ].
VAR_013925
Natural varianti72 – 721A → S Correlated with reduced enzyme activity; could be a risk allele for schizophrenia. 3 Publications
Corresponds to variant rs6267 [ dbSNP | Ensembl ].
VAR_013926
Natural varianti102 – 1021A → T.
Corresponds to variant rs5031015 [ dbSNP | Ensembl ].
VAR_020274
Natural varianti146 – 1461A → V.
Corresponds to variant rs4986871 [ dbSNP | Ensembl ].
VAR_020275
Natural varianti158 – 1581V → M in allele COMT*2; associated with low enzyme activity and thermolability; may increase the tendency to develop high blood pressure and abdominal obesity. 6 Publications
Corresponds to variant rs4680 [ dbSNP | Ensembl ].
VAR_005139

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5050Missing in isoform Soluble.
VSP_018778Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti245 – 2451Q → N AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M65212 mRNA. Translation: AAA68927.1.
M65213 mRNA. Translation: AAA68928.1.
M58525 mRNA. Translation: AAA68929.1.
Z26491 Genomic DNA. Translation: CAA81263.1.
AK290440 mRNA. Translation: BAF83129.1.
CR456422 mRNA. Translation: CAG30308.1.
CR456997 mRNA. Translation: CAG33278.1.
AY341246 Genomic DNA. Translation: AAP88929.1.
AC000080 Genomic DNA. No translation available.
AC000090 Genomic DNA. No translation available.
AC005663 Genomic DNA. No translation available.
CH471176 Genomic DNA. Translation: EAX03010.1.
BC011935 mRNA. Translation: AAH11935.1.
BC100018 mRNA. Translation: AAI00019.1.
CCDSiCCDS13770.1. [P21964-1]
CCDS46663.1. [P21964-2]
PIRiI37256. A38459.
RefSeqiNP_000745.1. NM_000754.3. [P21964-1]
NP_001128633.1. NM_001135161.1. [P21964-1]
NP_001128634.1. NM_001135162.1. [P21964-1]
NP_009294.1. NM_007310.2. [P21964-2]
XP_005261286.1. XM_005261229.1. [P21964-1]
UniGeneiHs.370408.
Hs.713616.

Genome annotation databases

EnsembliENST00000361682; ENSP00000354511; ENSG00000093010. [P21964-1]
ENST00000403710; ENSP00000385917; ENSG00000093010. [P21964-1]
ENST00000406520; ENSP00000385150; ENSG00000093010. [P21964-1]
ENST00000407537; ENSP00000384654; ENSG00000093010. [P21964-2]
ENST00000449653; ENSP00000416778; ENSG00000093010. [P21964-2]
GeneIDi1312.
KEGGihsa:1312.
UCSCiuc002zqu.3. human. [P21964-1]

Polymorphism databases

DMDMi116907.

Keywords - Coding sequence diversityi

Alternative initiation, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs
Wikipedia

Catechol-O-methyl transferase entry

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M65212 mRNA. Translation: AAA68927.1 .
M65213 mRNA. Translation: AAA68928.1 .
M58525 mRNA. Translation: AAA68929.1 .
Z26491 Genomic DNA. Translation: CAA81263.1 .
AK290440 mRNA. Translation: BAF83129.1 .
CR456422 mRNA. Translation: CAG30308.1 .
CR456997 mRNA. Translation: CAG33278.1 .
AY341246 Genomic DNA. Translation: AAP88929.1 .
AC000080 Genomic DNA. No translation available.
AC000090 Genomic DNA. No translation available.
AC005663 Genomic DNA. No translation available.
CH471176 Genomic DNA. Translation: EAX03010.1 .
BC011935 mRNA. Translation: AAH11935.1 .
BC100018 mRNA. Translation: AAI00019.1 .
CCDSi CCDS13770.1. [P21964-1 ]
CCDS46663.1. [P21964-2 ]
PIRi I37256. A38459.
RefSeqi NP_000745.1. NM_000754.3. [P21964-1 ]
NP_001128633.1. NM_001135161.1. [P21964-1 ]
NP_001128634.1. NM_001135162.1. [P21964-1 ]
NP_009294.1. NM_007310.2. [P21964-2 ]
XP_005261286.1. XM_005261229.1. [P21964-1 ]
UniGenei Hs.370408.
Hs.713616.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3A7E X-ray 2.80 A 51-264 [» ]
3BWM X-ray 1.98 A 52-265 [» ]
3BWY X-ray 1.30 A 52-265 [» ]
4PYI X-ray 1.35 A 51-271 [» ]
4PYJ X-ray 1.90 A 51-271 [» ]
4PYK X-ray 2.22 A 51-271 [» ]
ProteinModelPortali P21964.
SMRi P21964. Positions 52-265.
ModBasei Search...

Protein-protein interaction databases

BioGridi 107707. 10 interactions.
IntActi P21964. 6 interactions.
MINTi MINT-4529967.
STRINGi 9606.ENSP00000354511.

Chemistry

BindingDBi P21964.
ChEMBLi CHEMBL2023.
DrugBanki DB00190. Carbidopa.
DB00286. Conjugated Estrogens.
DB00255. Diethylstilbestrol.
DB00841. Dobutamine.
DB00988. Dopamine.
DB00494. Entacapone.
DB00158. Folic Acid.
DB00161. L-Valine.
DB01235. Levodopa.
DB00968. Methyldopa.
DB00745. Modafinil.
DB00295. Morphine.
DB00118. S-Adenosylmethionine.
DB00323. Tolcapone.
GuidetoPHARMACOLOGYi 2472.

PTM databases

PhosphoSitei P21964.

Polymorphism databases

DMDMi 116907.

2D gel databases

REPRODUCTION-2DPAGE IPI00375513.

Proteomic databases

MaxQBi P21964.
PaxDbi P21964.
PRIDEi P21964.

Protocols and materials databases

DNASUi 1312.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000361682 ; ENSP00000354511 ; ENSG00000093010 . [P21964-1 ]
ENST00000403710 ; ENSP00000385917 ; ENSG00000093010 . [P21964-1 ]
ENST00000406520 ; ENSP00000385150 ; ENSG00000093010 . [P21964-1 ]
ENST00000407537 ; ENSP00000384654 ; ENSG00000093010 . [P21964-2 ]
ENST00000449653 ; ENSP00000416778 ; ENSG00000093010 . [P21964-2 ]
GeneIDi 1312.
KEGGi hsa:1312.
UCSCi uc002zqu.3. human. [P21964-1 ]

Organism-specific databases

CTDi 1312.
GeneCardsi GC22P019929.
HGNCi HGNC:2228. COMT.
HPAi CAB011233.
HPA001169.
MIMi 103780. phenotype.
116790. gene+phenotype.
neXtProti NX_P21964.
Orphaneti 567. 22q11.2 deletion syndrome.
240863. Cisplatin toxicity.
3140. Schizophrenia.
240999. Susceptibility to deafness due to cisplatin treatment.
PharmGKBi PA117.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG4122.
HOGENOMi HOG000046392.
HOVERGENi HBG005376.
InParanoidi P21964.
KOi K00545.
OMAi CTHYSSY.
OrthoDBi EOG7PZRZJ.
PhylomeDBi P21964.
TreeFami TF329140.

Enzyme and pathway databases

BioCyci MetaCyc:HS01791-MONOMER.
BRENDAi 2.1.1.6. 2681.
Reactomei REACT_15511. Enzymatic degradation of Dopamine by monoamine oxidase.
REACT_15548. Enzymatic degradation of dopamine by COMT.
REACT_6946. Methylation.

Miscellaneous databases

ChiTaRSi COMT. human.
EvolutionaryTracei P21964.
GeneWikii Catechol-O-methyl_transferase.
GenomeRNAii 1312.
NextBioi 5365.
PROi P21964.
SOURCEi Search...

Gene expression databases

ArrayExpressi P21964.
Bgeei P21964.
CleanExi HS_COMT.
Genevestigatori P21964.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR025782. Catechol_O-MeTrfase.
IPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10509. PTHR10509. 1 hit.
Pfami PF01596. Methyltransf_3. 1 hit.
[Graphical view ]
PIRSFi PIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51682. SAM_OMT_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of human placental catechol-O-methyltransferase cDNA."
    Lundstroem K., Salminen M., Jalanko A., Savolainen R., Ulmanen I.
    DNA Cell Biol. 10:181-189(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Placenta.
  2. "Human catechol-O-methyltransferase: cloning and expression of the membrane-associated form."
    Bertocci B., Miggiano V., da Prada M., Dembic Z., Lahm H.-W., Malherbe P.
    Proc. Natl. Acad. Sci. U.S.A. 88:1416-1420(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-34.
  3. "Genomic organization of the human catechol O-methyltransferase gene and its expression from two distinct promoters."
    Tenhunen J., Salminen M., Lundstroem K., Kiviluoto T., Savolainen R., Ulmanen I.
    Eur. J. Biochem. 223:1049-1059(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT MET-158.
  6. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. NIEHS SNPs program
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS SER-72 AND MET-158.
  8. "The DNA sequence of human chromosome 22."
    Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
    , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
    Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT MET-158.
  10. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain and Skin.
  11. "Mass spectrometric analysis of human soluble catechol O-methyltransferase expressed in Escherichia coli. Identification of a product of ribosomal frameshifting and of reactive cysteines involved in S-adenosyl-L-methionine binding."
    Vilbois F., Caspers P., da Prada M., Lang G., Karrer C., Lahm H.W., Cesura A.M.
    Eur. J. Biochem. 222:377-386(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 52-61, MASS SPECTROMETRY.
  12. "Purification and partial sequence analysis of the soluble catechol-O-methyltransferase from human placenta: comparison to the rat liver enzyme."
    Tilgmann C., Kalkkinen N.
    Biochem. Biophys. Res. Commun. 174:995-1002(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 59-271.
    Tissue: Placenta.
  13. "Cell-free synthesis of rat and human catechol O-methyltransferase. Insertion of the membrane-bound form into microsomal membranes in vitro."
    Ulmanen I., Lundstroem K.
    Eur. J. Biochem. 202:1013-1020(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF THE TWO FORMS.
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Crystal structures of human 108V and 108M catechol O-methyltransferase."
    Rutherford K., Le Trong I., Stenkamp R.E., Parson W.W.
    J. Mol. Biol. 380:120-130(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 52-264 OF MUTANTS VAL-108 AND MET-108 IN COMPLEX WITH SUBSTRATE ANALOG 3,5-DINITROCATECHOL; MAGNESIUM AND S-ADENOSYL-L-METHIONINE.
  16. "Human catechol-O-methyltransferase pharmacogenetics: description of a functional polymorphism and its potential application to neuropsychiatric disorders."
    Lachman H.M., Papolos D.F., Saito T., Yu Y.-M., Szumlanski C.L., Weinshilboum R.M.
    Pharmacogenetics 6:243-250(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT COMT*2 MET-158.
  17. "Association between the functional variant of the catechol-O-methyltransferase (COMT) gene and type 1 alcoholism."
    Tiihonen J., Hallikainen T., Lachman H., Saito T., Volavka J., Kauhanen J., Salonen J.T., Ryynaenen O.-P., Koulu M., Karvonen M.K., Pohjalainen T., Syvaelahti E., Hietala J.
    Mol. Psychiatry 4:286-289(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INVOLVEMENT IN SUSCEPTIBILITY TO ALCOHOLISM.
  18. Cited for: VARIANTS SER-34 AND SER-72.
  19. "Association of Ala72Ser polymorphism with COMT enzyme activity and the risk of schizophrenia in Koreans."
    Lee S.-G., Joo Y., Kim B., Chung S., Kim H.-L., Lee I., Choi B., Kim C., Song K.
    Hum. Genet. 116:319-328(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF VARIANT SER-72, POSSIBLE INVOLVEMENT IN SCHIZOPHRENIA.
  20. "The V108M mutation decreases the structural stability of catechol O-methyltransferase."
    Rutherford K., Alphandery E., McMillan A., Daggett V., Parson W.W.
    Biochim. Biophys. Acta 1784:1098-1105(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF VARIANT COMT*2 MET-158.
  21. "Catechol O-methyltransferase val158-met polymorphism is associated with abdominal obesity and blood pressure in men."
    Annerbrink K., Westberg L., Nilsson S., Rosmond R., Holm G., Eriksson E.
    Metabolism 57:708-711(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT COMT*2 MET-158.

Entry informationi

Entry nameiCOMT_HUMAN
AccessioniPrimary (citable) accession number: P21964
Secondary accession number(s): A8MPV9, Q6IB07, Q6ICE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: May 1, 1992
Last modified: September 3, 2014
This is version 168 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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