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P21958

- TAP1_MOUSE

UniProt

P21958 - TAP1_MOUSE

Protein

Antigen peptide transporter 1

Gene

Tap1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 3 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi514 – 5218ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ADP binding Source: Ensembl
    2. ATPase activity, coupled to transmembrane movement of substances Source: RefGenome
    3. ATP binding Source: UniProtKB-KW
    4. MHC class I protein binding Source: MGI
    5. peptide transporter activity Source: InterPro

    GO - Biological processi

    1. antigen processing and presentation of endogenous peptide antigen via MHC class I Source: Ensembl
    2. cytosol to ER transport Source: Ensembl
    3. defense response Source: MGI
    4. intracellular transport of viral protein in host cell Source: Ensembl
    5. transmembrane transport Source: RefGenome

    Keywords - Biological processi

    Adaptive immunity, Immunity, Peptide transport, Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_197102. ER-Phagosome pathway.
    REACT_199105. ER-Phagosome pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Antigen peptide transporter 1
    Short name:
    APT1
    Alternative name(s):
    ATP-binding cassette sub-family B member 2
    Histocompatibility antigen modifier 1
    Peptide transporter TAP1
    Gene namesi
    Name:Tap1
    Synonyms:Abcb2, Ham-1, Ham1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:98483. Tap1.

    Subcellular locationi

    Endoplasmic reticulum membrane; Multi-pass membrane protein
    Note: The transmembrane segments seem to form a pore in the membrane.

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: Ensembl
    2. integral component of membrane Source: RefGenome
    3. mitochondrion Source: MGI
    4. phagocytic vesicle membrane Source: Reactome

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 724724Antigen peptide transporter 1PRO_0000093327Add
    BLAST

    Proteomic databases

    MaxQBiP21958.
    PaxDbiP21958.
    PRIDEiP21958.

    PTM databases

    PhosphoSiteiP21958.

    Expressioni

    Gene expression databases

    ArrayExpressiP21958.
    BgeeiP21958.
    GenevestigatoriP21958.

    Interactioni

    Subunit structurei

    Heterodimer of TAP1 and TAP2. Interacts with PSMB5 and PSMB8 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi203966. 1 interaction.
    IntActiP21958. 2 interactions.
    MINTiMINT-4136864.

    Structurei

    3D structure databases

    ProteinModelPortaliP21958.
    SMRiP21958. Positions 151-718.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 364LumenalSequence Analysis
    Topological domaini60 – 689CytoplasmicSequence Analysis
    Topological domaini90 – 10920LumenalSequence AnalysisAdd
    BLAST
    Topological domaini131 – 16232CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini184 – 20320LumenalSequence AnalysisAdd
    BLAST
    Topological domaini225 – 27450CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini296 – 3049LumenalSequence Analysis
    Topological domaini326 – 39469CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini416 – 4194LumenalSequence Analysis
    Topological domaini441 – 724284CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221Helical; Name=1PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei37 – 5923Helical; Name=2PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei69 – 8921Helical; Name=3PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei110 – 13021Helical; Name=4PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei163 – 18321Helical; Name=5PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei204 – 22421Helical; Name=6PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei275 – 29521Helical; Name=7PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei305 – 32521Helical; Name=8PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei395 – 41521Helical; Name=9PROSITE-ProRule annotationAdd
    BLAST
    Transmembranei420 – 44021Helical; Name=10PROSITE-ProRule annotationAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini163 – 446284ABC transmembrane type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini479 – 718240ABC transporterPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni351 – 39646Involved in peptide-binding siteBy similarityAdd
    BLAST
    Regioni429 – 46335Involved in peptide-binding siteBy similarityAdd
    BLAST

    Domaini

    The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.By similarity

    Sequence similaritiesi

    Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
    Contains 1 ABC transporter domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1132.
    GeneTreeiENSGT00550000074497.
    HOVERGENiHBG008358.
    InParanoidiQ62427.
    KOiK05653.
    OMAiCLGSEIR.
    OrthoDBiEOG7Z3F4H.
    PhylomeDBiP21958.
    TreeFamiTF105197.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR013305. ABC_B2.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR005293. Ag_transporter2.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00664. ABC_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view]
    PRINTSiPR01896. TAP1PROTEIN.
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF90123. SSF90123. 1 hit.
    TIGRFAMsiTIGR00958. 3a01208. 1 hit.
    PROSITEiPS50929. ABC_TM1F. 1 hit.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21958-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAHVWLAAA LLLLVDWLLL RPMLPGIFSL LVPEVPLLRV WVVGLSRWAI    50
    LGLGVRGVLG VTAGAHGWLA ALQPLVAALS LALPGLALFR ELAAWGTLRE 100
    GDSAGLLYWN SRPDAFAISY VAALPAAALW HKLGSLWAPS GNRDAGDMLC 150
    RMLGFLGPKK RRLYLVLVLL ILSCLGEMAI PFFTGRITDW ILQDKTVPSF 200
    TRNIWLMSIL TIASTALEFA SDGIYNITMG HMHGRVHREV FRAVLRQETG 250
    FFLKNPAGSI TSRVTEDTAN VCESISGTLS LLLWYLGRAL CLLVFMFWGS 300
    PYLTLVTLIN LPLLFLLPKK LGKVHQSLAV KVQESLAKST QVALEALSAM 350
    PTVRSFANEE GEAQKFRQKL EEMKTLNKKE ALAYVAEVWT TSVSGMLLKV 400
    GILYLGGQLV IRGAVSSGNL VSFVLYQLQF TQAVQVLLSL YPSMQKAVGS 450
    SEKIFEYLDR TPCSPLSGSL APSNMKGLVE FQDVSFAYPN QPKVQVLQGL 500
    TFTLHPGTVT ALVGPNGSGK STVAALLQNL YQPTGGQLLL DGQCLVQYDH 550
    HYLHTQVAAV GQEPLLFGRS FRENIAYGLN RTPTMEEITA VAVESGAHDF 600
    ISGFPQGYDT EVGETGNQLS GGQRQAVALA RALIRKPLLL ILDDATSALD 650
    AGNQLRVQRL LYESPKRASR TVLLITQQLS LAEQAHHILF LREGSVGEQG 700
    THLQLMKRGG CYRAMVEALA APAD 724
    Length:724
    Mass (Da):78,864
    Last modified:June 28, 2011 - v3
    Checksum:iA08E40F9F5FBAE9D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti277 – 2771G → D in AAB41962. (PubMed:9164943)Curated
    Sequence conflicti277 – 2771G → D in AAA39570. (PubMed:2270487)Curated
    Sequence conflicti277 – 2771G → D in CAA42178. 1 PublicationCurated
    Sequence conflicti355 – 3551S → Q in CAA42178. 1 PublicationCurated
    Sequence conflicti414 – 4141A → T in AAB41962. (PubMed:9164943)Curated
    Sequence conflicti414 – 4141A → T in AAA39570. (PubMed:2270487)Curated
    Sequence conflicti414 – 4141A → T in CAA42178. 1 PublicationCurated
    Sequence conflicti481 – 4811F → L in CAA42178. 1 PublicationCurated
    Sequence conflicti544 – 5441C → R in AAB41962. (PubMed:9164943)Curated
    Sequence conflicti544 – 5441C → R in AAA39570. (PubMed:2270487)Curated
    Sequence conflicti544 – 5441C → R in CAA42178. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60018 mRNA. Translation: AAB41962.1.
    U60019 mRNA. Translation: AAB41963.1.
    AK162731 mRNA. Translation: BAE37041.1.
    AF027865 Genomic DNA. Translation: AAB81534.1.
    CH466660 Genomic DNA. Translation: EDL10260.1.
    M55637 mRNA. Translation: AAA39570.1.
    X59615 mRNA. Translation: CAA42178.1.
    CCDSiCCDS28643.1.
    PIRiA37779.
    JC2020.
    RefSeqiNP_038711.2. NM_013683.2.
    UniGeneiMm.482076.

    Genome annotation databases

    EnsembliENSMUST00000170086; ENSMUSP00000128401; ENSMUSG00000037321.
    GeneIDi21354.
    KEGGimmu:21354.
    UCSCiuc008cbu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60018 mRNA. Translation: AAB41962.1 .
    U60019 mRNA. Translation: AAB41963.1 .
    AK162731 mRNA. Translation: BAE37041.1 .
    AF027865 Genomic DNA. Translation: AAB81534.1 .
    CH466660 Genomic DNA. Translation: EDL10260.1 .
    M55637 mRNA. Translation: AAA39570.1 .
    X59615 mRNA. Translation: CAA42178.1 .
    CCDSi CCDS28643.1.
    PIRi A37779.
    JC2020.
    RefSeqi NP_038711.2. NM_013683.2.
    UniGenei Mm.482076.

    3D structure databases

    ProteinModelPortali P21958.
    SMRi P21958. Positions 151-718.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203966. 1 interaction.
    IntActi P21958. 2 interactions.
    MINTi MINT-4136864.

    PTM databases

    PhosphoSitei P21958.

    Proteomic databases

    MaxQBi P21958.
    PaxDbi P21958.
    PRIDEi P21958.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000170086 ; ENSMUSP00000128401 ; ENSMUSG00000037321 .
    GeneIDi 21354.
    KEGGi mmu:21354.
    UCSCi uc008cbu.2. mouse.

    Organism-specific databases

    CTDi 6890.
    MGIi MGI:98483. Tap1.

    Phylogenomic databases

    eggNOGi COG1132.
    GeneTreei ENSGT00550000074497.
    HOVERGENi HBG008358.
    InParanoidi Q62427.
    KOi K05653.
    OMAi CLGSEIR.
    OrthoDBi EOG7Z3F4H.
    PhylomeDBi P21958.
    TreeFami TF105197.

    Enzyme and pathway databases

    Reactomei REACT_197102. ER-Phagosome pathway.
    REACT_199105. ER-Phagosome pathway.

    Miscellaneous databases

    NextBioi 300552.
    PROi P21958.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P21958.
    Bgeei P21958.
    Genevestigatori P21958.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR013305. ABC_B2.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR005293. Ag_transporter2.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00664. ABC_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view ]
    PRINTSi PR01896. TAP1PROTEIN.
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF90123. SSF90123. 1 hit.
    TIGRFAMsi TIGR00958. 3a01208. 1 hit.
    PROSITEi PS50929. ABC_TM1F. 1 hit.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Allelic variation in the mouse Tap-1 and Tap-2 transporter genes."
      Marusina K., Iyer M., Monaco J.J.
      J. Immunol. 158:5251-5256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: WEHI-3.
      Tissue: T-cell lymphoma.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Thymus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Transport protein genes in the murine MHC: possible implications for antigen processing."
      Monaco J.J., Cho S., Attaya M.
      Science 250:1723-1726(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 148-724.
    6. Sun X.Y., Zhou J., Frazer I.
      Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 148-724.
      Strain: BALB/c.

    Entry informationi

    Entry nameiTAP1_MOUSE
    AccessioniPrimary (citable) accession number: P21958
    Secondary accession number(s): Q62427
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 144 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3