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P21958 (TAP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Antigen peptide transporter 1

Short name=APT1
Alternative name(s):
ATP-binding cassette sub-family B member 2
Histocompatibility antigen modifier 1
Peptide transporter TAP1
Gene names
Name:Tap1
Synonyms:Abcb2, Ham-1, Ham1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length724 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin By similarity.

Subunit structure

Heterodimer of TAP1 and TAP2. Interacts with PSMB5 and PSMB8 By similarity.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Note: The transmembrane segments seem to form a pore in the membrane.

Domain

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2 By similarity.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily. [View classification]

Contains 1 ABC transmembrane type-1 domain.

Contains 1 ABC transporter domain.

Ontologies

Keywords
   Biological processAdaptive immunity
Immunity
Peptide transport
Protein transport
Transport
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of endogenous peptide antigen via MHC class I

Inferred from electronic annotation. Source: Ensembl

cytosol to ER transport

Inferred from electronic annotation. Source: Ensembl

defense response

Inferred from mutant phenotype PubMed 1473153. Source: MGI

intracellular transport of viral protein in host cell

Inferred from electronic annotation. Source: Ensembl

oligopeptide transmembrane transport

Inferred from Biological aspect of Ancestor. Source: GOC

peptide transport

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of T cell mediated cytotoxicity

Inferred from Biological aspect of Ancestor. Source: RefGenome

transmembrane transport

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Cellular_componentTAP complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

mitochondrion

Inferred from direct assay PubMed 18614015. Source: MGI

phagocytic vesicle membrane

Traceable author statement. Source: Reactome

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionADP binding

Inferred from electronic annotation. Source: Ensembl

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MHC class I protein binding

Inferred from physical interaction PubMed 14735325. Source: MGI

TAP1 binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

TAP2 binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

oligopeptide-transporting ATPase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

peptide antigen binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

tapasin binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 724724Antigen peptide transporter 1
PRO_0000093327

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221Helical; Name=1; Potential
Topological domain33 – 364Lumenal Potential
Transmembrane37 – 5923Helical; Name=2; Potential
Topological domain60 – 689Cytoplasmic Potential
Transmembrane69 – 8921Helical; Name=3; Potential
Topological domain90 – 10920Lumenal Potential
Transmembrane110 – 13021Helical; Name=4; Potential
Topological domain131 – 16232Cytoplasmic Potential
Transmembrane163 – 18321Helical; Name=5; Potential
Topological domain184 – 20320Lumenal Potential
Transmembrane204 – 22421Helical; Name=6; Potential
Topological domain225 – 27450Cytoplasmic Potential
Transmembrane275 – 29521Helical; Name=7; Potential
Topological domain296 – 3049Lumenal Potential
Transmembrane305 – 32521Helical; Name=8; Potential
Topological domain326 – 39469Cytoplasmic Potential
Transmembrane395 – 41521Helical; Name=9; Potential
Topological domain416 – 4194Lumenal Potential
Transmembrane420 – 44021Helical; Name=10; Potential
Topological domain441 – 724284Cytoplasmic Potential
Domain163 – 446284ABC transmembrane type-1
Domain479 – 718240ABC transporter
Nucleotide binding514 – 5218ATP Potential
Region351 – 39646Involved in peptide-binding site By similarity
Region429 – 46335Involved in peptide-binding site By similarity

Experimental info

Sequence conflict2771G → D in AAB41962. Ref.1
Sequence conflict2771G → D in AAA39570. Ref.5
Sequence conflict2771G → D in CAA42178. Ref.6
Sequence conflict3551S → Q in CAA42178. Ref.6
Sequence conflict4141A → T in AAB41962. Ref.1
Sequence conflict4141A → T in AAA39570. Ref.5
Sequence conflict4141A → T in CAA42178. Ref.6
Sequence conflict4811F → L in CAA42178. Ref.6
Sequence conflict5441C → R in AAB41962. Ref.1
Sequence conflict5441C → R in AAA39570. Ref.5
Sequence conflict5441C → R in CAA42178. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P21958 [UniParc].

Last modified June 28, 2011. Version 3.
Checksum: A08E40F9F5FBAE9D

FASTA72478,864
        10         20         30         40         50         60 
MAAHVWLAAA LLLLVDWLLL RPMLPGIFSL LVPEVPLLRV WVVGLSRWAI LGLGVRGVLG 

        70         80         90        100        110        120 
VTAGAHGWLA ALQPLVAALS LALPGLALFR ELAAWGTLRE GDSAGLLYWN SRPDAFAISY 

       130        140        150        160        170        180 
VAALPAAALW HKLGSLWAPS GNRDAGDMLC RMLGFLGPKK RRLYLVLVLL ILSCLGEMAI 

       190        200        210        220        230        240 
PFFTGRITDW ILQDKTVPSF TRNIWLMSIL TIASTALEFA SDGIYNITMG HMHGRVHREV 

       250        260        270        280        290        300 
FRAVLRQETG FFLKNPAGSI TSRVTEDTAN VCESISGTLS LLLWYLGRAL CLLVFMFWGS 

       310        320        330        340        350        360 
PYLTLVTLIN LPLLFLLPKK LGKVHQSLAV KVQESLAKST QVALEALSAM PTVRSFANEE 

       370        380        390        400        410        420 
GEAQKFRQKL EEMKTLNKKE ALAYVAEVWT TSVSGMLLKV GILYLGGQLV IRGAVSSGNL 

       430        440        450        460        470        480 
VSFVLYQLQF TQAVQVLLSL YPSMQKAVGS SEKIFEYLDR TPCSPLSGSL APSNMKGLVE 

       490        500        510        520        530        540 
FQDVSFAYPN QPKVQVLQGL TFTLHPGTVT ALVGPNGSGK STVAALLQNL YQPTGGQLLL 

       550        560        570        580        590        600 
DGQCLVQYDH HYLHTQVAAV GQEPLLFGRS FRENIAYGLN RTPTMEEITA VAVESGAHDF 

       610        620        630        640        650        660 
ISGFPQGYDT EVGETGNQLS GGQRQAVALA RALIRKPLLL ILDDATSALD AGNQLRVQRL 

       670        680        690        700        710        720 
LYESPKRASR TVLLITQQLS LAEQAHHILF LREGSVGEQG THLQLMKRGG CYRAMVEALA 


APAD 

« Hide

References

« Hide 'large scale' references
[1]"Allelic variation in the mouse Tap-1 and Tap-2 transporter genes."
Marusina K., Iyer M., Monaco J.J.
J. Immunol. 158:5251-5256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: WEHI-3.
Tissue: T-cell lymphoma.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Thymus.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Transport protein genes in the murine MHC: possible implications for antigen processing."
Monaco J.J., Cho S., Attaya M.
Science 250:1723-1726(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 148-724.
[6]Sun X.Y., Zhou J., Frazer I.
Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 148-724.
Strain: BALB/c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U60018 mRNA. Translation: AAB41962.1.
U60019 mRNA. Translation: AAB41963.1.
AK162731 mRNA. Translation: BAE37041.1.
AF027865 Genomic DNA. Translation: AAB81534.1.
CH466660 Genomic DNA. Translation: EDL10260.1.
M55637 mRNA. Translation: AAA39570.1.
X59615 mRNA. Translation: CAA42178.1.
CCDSCCDS28643.1.
PIRA37779.
JC2020.
RefSeqNP_038711.2. NM_013683.2.
UniGeneMm.482076.

3D structure databases

ProteinModelPortalP21958.
SMRP21958. Positions 151-718.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203966. 1 interaction.
IntActP21958. 2 interactions.
MINTMINT-4136864.

PTM databases

PhosphoSiteP21958.

Proteomic databases

MaxQBP21958.
PaxDbP21958.
PRIDEP21958.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000170086; ENSMUSP00000128401; ENSMUSG00000037321.
GeneID21354.
KEGGmmu:21354.
UCSCuc008cbu.2. mouse.

Organism-specific databases

CTD6890.
MGIMGI:98483. Tap1.

Phylogenomic databases

eggNOGCOG1132.
GeneTreeENSGT00550000074497.
HOVERGENHBG008358.
InParanoidQ62427.
KOK05653.
OMACLGSEIR.
OrthoDBEOG7Z3F4H.
PhylomeDBP21958.
TreeFamTF105197.

Gene expression databases

ArrayExpressP21958.
BgeeP21958.
GenevestigatorP21958.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR013305. ABC_B2.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR005293. Ag_transporter2.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
PRINTSPR01896. TAP1PROTEIN.
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsTIGR00958. 3a01208. 1 hit.
PROSITEPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio300552.
PROP21958.
SOURCESearch...

Entry information

Entry nameTAP1_MOUSE
AccessionPrimary (citable) accession number: P21958
Secondary accession number(s): Q62427
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 28, 2011
Last modified: July 9, 2014
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot