Transcriptional repressor OPI1 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P21957 (OPI1_YEAST)

Last modified November 24, 2009. Version 85. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Transcriptional repressor OPI1
Alternative name(s):
    Overproducer of inositol protein 1
    Negative regulator of phospholipid biosynthesis

Gene names

Name:	OPI1
Ordered Locus Names:	YHL020C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Negative regulator of the transcriptional complex INO2-INO4 in response to phospholipid precursor availability. When precursors become limiting, OPI1 is retained at the endoplasmic reticulum (ER) and INO2-INO4 activates INO1 and other genes required for phospholipid biosynthesis, whereas abundant precursor availability results in targeting of OPI1 to the nucleus to repress transcription of these genes. Binds directly to phosphatidic acid, which is required for ER targeting and may act as sensing mechanism for precursor availability, as phosphatidic acid becomes rapidly depleted upon phospholipid biosynthesis. Ref.8
Subunit structure	Interacts with SCS2. Ref.4 Ref.7
Subcellular location	Endoplasmic reticulum. Nucleus. Note: Maintained at the endoplasmic reticulum by SCS2. In response to elevated inositol levels. Ref.8 Ref.4 Ref.5
Domain	The FFAT motif is required for interaction with SCS2 and proper localization of the protein. Ref.4
Miscellaneous	Present with 1281 molecules/cell in log phase SD medium. Ref.6

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Ontologies

Keywords
Biological process	Phospholipid biosynthesis Transcription Transcription regulation
Cellular component	Endoplasmic reticulum Nucleus
Ligand	DNA-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	endoplasmic reticulum unfolded protein response Inferred from mutant phenotype. Source: SGD negative regulation of transcription from RNA polymerase II promoter Ref.1 Inferred from mutant phenotype. Source: SGD phospholipid biosynthetic process Ref.1 Inferred from mutant phenotype. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Ref.8 Inferred from direct assay. Source: SGD nuclear membrane Ref.7 Inferred from direct assay. Source: SGD nucleoplasm Ref.7 Inferred from direct assay. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW transcription corepressor activity Inferred from physical interaction. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
INO2	P26798	1	EBI-12555,EBI-9262
SIN3	P22579	3	EBI-12555,EBI-17160

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 404

404

Transcriptional repressor OPI1

PRO_0000058061

Regions

Domain

139 – 160

Leucine-zipper

DNA binding

109 – 138

Basic motif

Motif

200 – 206

FFAT

Compositional bias

89 – 93

Poly-Asp

Compositional bias

193 – 198

Poly-Asp

Compositional bias

286 – 298

Poly-Gln

Compositional bias

306 – 310

Poly-Gln

Compositional bias

377 – 381

Poly-Gln

Compositional bias

384 – 387

Poly-Gln

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.11

Modified residue

230

Phosphoserine Ref.11 Ref.10

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Sequences

Sequence

Length

Mass (Da)

Tools

P21957-1 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 59E55D7BE7122EDA
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FASTA

404

46,065

        10         20         30         40         50         60 
MSENQRLGLS EEEVEAAEVL GVLKQSCRQK SQPSEDVSQA DKMPASESST TPLNILDRVS 

        70         80         90        100        110        120 
NKIISNVVTF YDEINTNKRP LKSIGRLLDD DDDEHDDYDY NDDEFFTNKR QKLSRAIAKG 

       130        140        150        160        170        180 
KDNLKEYKLN MSIESKKRLV TCLHLLKLAN KQLSDKISCL QDLVEKEQVH PLHKQDGNAR 

       190        200        210        220        230        240 
TTTGAGEDET SSDEDDDDEE FFDASEQVNA SEQSIVVKME VVGTVKKVYS LISKFTANSL 

       250        260        270        280        290        300 
PEPARSQVRE SLLNLPTNWF DSVHSTSLPH HASFHYANCE EQKVEQQQQQ QQQQQQQQLL 

       310        320        330        340        350        360 
QQQLLQQQQQ KRNKDGDDSA SPSSSVTANG KVLILAKESL EMVRNVMGVV DSTLGKAEEW 

       370        380        390        400 
VKQKQEVKEM IRERFLQQQQ QYRQQQQKDG NYVKPSQDNV DSKD

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The OPI1 gene of Saccharomyces cerevisiae, a negative regulator of phospholipid biosynthesis, encodes a protein containing polyglutamine tracts and a leucine zipper." White M.J., Hirsch J.P., Henry S.A. J. Biol. Chem. 266:863-872(1991) [PubMed: 1985968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP." Loewen C.J.R., Roy A., Levine T.P. EMBO J. 22:2025-2035(2003) [PubMed: 12727870] [Abstract] Cited for: DOMAIN FFAT MOTIF, INTERACTION WITH SCS2, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Gene recruitment of the activated INO1 locus to the nuclear membrane." Brickner J.H., Walter P. PLoS Biol. 2:1843-1852(2004) [PubMed: 15455074] [Abstract] Cited for: INTERACTION WITH SCS2.
[8]	"Phospholipid metabolism regulated by a transcription factor sensing phosphatidic acid." Loewen C.J.R., Gaspar M.L., Jesch S.A., Delon C., Ktistakis N.T., Henry S.A., Levine T.P. Science 304:1644-1647(2004) [PubMed: 15192221] [Abstract] Cited for: FUNCTION, PHOSPHATIDIC ACID-BINDING, SUBCELLULAR LOCATION.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49 AND SER-230, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	M57383 Genomic DNA. Translation: AAA34828.1. U11582 Genomic DNA. Translation: AAB65073.1. AY558103 Genomic DNA. Translation: AAS56429.1.
PIR	S13741.
RefSeq	NP_011843.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5505N.
IntAct	P21957. 13 interactions.
STRING	P21957.
Genome annotation databases
Ensembl	YHL020C; YHL020C; YHL020C; Saccharomyces cerevisiae. [Genome view]
GeneID	856366.
KEGG	sce:YHL020C.
NMPDR	fig\|4932.3.peg.2981.
Organism-specific databases
CYGD	YHL020c.
SGD	S000001012. OPI1.
Phylogenomic databases
HOGENOM	P21957.
OMA	KEMIRER
OrthoDB	EOG9F7Q2R
Gene expression databases
ArrayExpress	P21957.
Genevestigator	P21957.
GermOnline	YHL020C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013927. TF_Opi1. [Graphical view]
Pfam	PF08618. Opi1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	981834.

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Entry information

Entry name

OPI1_YEAST

Accession

Primary (citable) accession number: P21957

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	November 24, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents