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P21957

- OPI1_YEAST

UniProt

P21957 - OPI1_YEAST

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Protein

Transcriptional repressor OPI1

Gene

OPI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Negative regulator of the transcriptional complex INO2-INO4 in response to phospholipid precursor availability. When precursors become limiting, OPI1 is retained at the endoplasmic reticulum (ER) and INO2-INO4 activates INO1 and other genes required for phospholipid biosynthesis, whereas abundant precursor availability results in targeting of OPI1 to the nucleus to repress transcription of these genes. Binds directly to phosphatidic acid, which is required for ER targeting and may act as sensing mechanism for precursor availability, as phosphatidic acid becomes rapidly depleted upon phospholipid biosynthesis.1 Publication

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. transcription corepressor activity Source: SGD

GO - Biological processi

  1. endoplasmic reticulum unfolded protein response Source: SGD
  2. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  3. phospholipid biosynthetic process Source: SGD
  4. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31040-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor OPI1
Alternative name(s):
Negative regulator of phospholipid biosynthesis
Overproducer of inositol protein 1
Gene namesi
Name:OPI1
Ordered Locus Names:YHL020C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VIII

Organism-specific databases

CYGDiYHL020c.
SGDiS000001012. OPI1.

Subcellular locationi

Endoplasmic reticulum. Nucleus
Note: Maintained at the endoplasmic reticulum by SCS2. In response to elevated inositol levels.

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. nuclear envelope Source: SGD
  3. nuclear membrane Source: SGD
  4. nucleoplasm Source: SGD
  5. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 404404Transcriptional repressor OPI1PRO_0000058061Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21957.
PaxDbiP21957.

Expressioni

Gene expression databases

GenevestigatoriP21957.

Interactioni

Subunit structurei

Interacts with SCS2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SIN3P225795EBI-12555,EBI-17160

Protein-protein interaction databases

BioGridi36403. 170 interactions.
DIPiDIP-5505N.
IntActiP21957. 8 interactions.
MINTiMINT-491924.
STRINGi4932.YHL020C.

Structurei

3D structure databases

ProteinModelPortaliP21957.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni109 – 13830Basic motifAdd
BLAST
Regioni139 – 16022Leucine-zipperAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi200 – 2067FFAT

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi89 – 935Poly-Asp
Compositional biasi193 – 1986Poly-Asp
Compositional biasi286 – 29813Poly-GlnAdd
BLAST
Compositional biasi306 – 3105Poly-Gln
Compositional biasi377 – 3815Poly-Gln
Compositional biasi384 – 3874Poly-Gln

Domaini

The FFAT motif is required for interaction with SCS2 and proper localization of the protein.1 Publication

Phylogenomic databases

eggNOGiNOG46650.
InParanoidiP21957.
KOiK09062.
OMAiHYANCEE.
OrthoDBiEOG7CK3HM.

Family and domain databases

InterProiIPR013927. TF_Opi1.
[Graphical view]
PfamiPF08618. Opi1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21957-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSENQRLGLS EEEVEAAEVL GVLKQSCRQK SQPSEDVSQA DKMPASESST
60 70 80 90 100
TPLNILDRVS NKIISNVVTF YDEINTNKRP LKSIGRLLDD DDDEHDDYDY
110 120 130 140 150
NDDEFFTNKR QKLSRAIAKG KDNLKEYKLN MSIESKKRLV TCLHLLKLAN
160 170 180 190 200
KQLSDKISCL QDLVEKEQVH PLHKQDGNAR TTTGAGEDET SSDEDDDDEE
210 220 230 240 250
FFDASEQVNA SEQSIVVKME VVGTVKKVYS LISKFTANSL PEPARSQVRE
260 270 280 290 300
SLLNLPTNWF DSVHSTSLPH HASFHYANCE EQKVEQQQQQ QQQQQQQQLL
310 320 330 340 350
QQQLLQQQQQ KRNKDGDDSA SPSSSVTANG KVLILAKESL EMVRNVMGVV
360 370 380 390 400
DSTLGKAEEW VKQKQEVKEM IRERFLQQQQ QYRQQQQKDG NYVKPSQDNV

DSKD
Length:404
Mass (Da):46,065
Last modified:August 1, 1991 - v1
Checksum:i59E55D7BE7122EDA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57383 Genomic DNA. Translation: AAA34828.1.
U11582 Genomic DNA. Translation: AAB65073.1.
AY558103 Genomic DNA. Translation: AAS56429.1.
BK006934 Genomic DNA. Translation: DAA06665.1.
PIRiS13741.
RefSeqiNP_011843.1. NM_001179100.1.

Genome annotation databases

EnsemblFungiiYHL020C; YHL020C; YHL020C.
GeneIDi856366.
KEGGisce:YHL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M57383 Genomic DNA. Translation: AAA34828.1 .
U11582 Genomic DNA. Translation: AAB65073.1 .
AY558103 Genomic DNA. Translation: AAS56429.1 .
BK006934 Genomic DNA. Translation: DAA06665.1 .
PIRi S13741.
RefSeqi NP_011843.1. NM_001179100.1.

3D structure databases

ProteinModelPortali P21957.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36403. 170 interactions.
DIPi DIP-5505N.
IntActi P21957. 8 interactions.
MINTi MINT-491924.
STRINGi 4932.YHL020C.

Proteomic databases

MaxQBi P21957.
PaxDbi P21957.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YHL020C ; YHL020C ; YHL020C .
GeneIDi 856366.
KEGGi sce:YHL020C.

Organism-specific databases

CYGDi YHL020c.
SGDi S000001012. OPI1.

Phylogenomic databases

eggNOGi NOG46650.
InParanoidi P21957.
KOi K09062.
OMAi HYANCEE.
OrthoDBi EOG7CK3HM.

Enzyme and pathway databases

BioCyci YEAST:G3O-31040-MONOMER.

Miscellaneous databases

NextBioi 981834.

Gene expression databases

Genevestigatori P21957.

Family and domain databases

InterProi IPR013927. TF_Opi1.
[Graphical view ]
Pfami PF08618. Opi1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The OPI1 gene of Saccharomyces cerevisiae, a negative regulator of phospholipid biosynthesis, encodes a protein containing polyglutamine tracts and a leucine zipper."
    White M.J., Hirsch J.P., Henry S.A.
    J. Biol. Chem. 266:863-872(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP."
    Loewen C.J.R., Roy A., Levine T.P.
    EMBO J. 22:2025-2035(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN FFAT MOTIF, INTERACTION WITH SCS2, SUBCELLULAR LOCATION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Gene recruitment of the activated INO1 locus to the nuclear membrane."
    Brickner J.H., Walter P.
    PLoS Biol. 2:1843-1852(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SCS2.
  9. "Phospholipid metabolism regulated by a transcription factor sensing phosphatidic acid."
    Loewen C.J.R., Gaspar M.L., Jesch S.A., Delon C., Ktistakis N.T., Henry S.A., Levine T.P.
    Science 304:1644-1647(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHATIDIC ACID-BINDING, SUBCELLULAR LOCATION.
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOPI1_YEAST
AccessioniPrimary (citable) accession number: P21957
Secondary accession number(s): D3DKP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 29, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1281 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

External Data

Dasty 3