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P21957 (OPI1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional repressor OPI1
Alternative name(s):
Negative regulator of phospholipid biosynthesis
Overproducer of inositol protein 1
Gene names
Name:OPI1
Ordered Locus Names:YHL020C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negative regulator of the transcriptional complex INO2-INO4 in response to phospholipid precursor availability. When precursors become limiting, OPI1 is retained at the endoplasmic reticulum (ER) and INO2-INO4 activates INO1 and other genes required for phospholipid biosynthesis, whereas abundant precursor availability results in targeting of OPI1 to the nucleus to repress transcription of these genes. Binds directly to phosphatidic acid, which is required for ER targeting and may act as sensing mechanism for precursor availability, as phosphatidic acid becomes rapidly depleted upon phospholipid biosynthesis. Ref.9

Subunit structure

Interacts with SCS2. Ref.5 Ref.8

Subcellular location

Endoplasmic reticulum. Nucleus. Note: Maintained at the endoplasmic reticulum by SCS2. In response to elevated inositol levels. Ref.5 Ref.6 Ref.9

Domain

The FFAT motif is required for interaction with SCS2 and proper localization of the protein. Ref.5

Miscellaneous

Present with 1281 molecules/cell in log phase SD medium.

Ontologies

Keywords
   Biological processLipid biosynthesis
Lipid metabolism
Phospholipid biosynthesis
Phospholipid metabolism
Transcription
Transcription regulation
   Cellular componentEndoplasmic reticulum
Nucleus
   LigandDNA-binding
   Molecular functionRepressor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processendoplasmic reticulum unfolded protein response

Inferred from mutant phenotype PubMed 16582425. Source: SGD

negative regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype Ref.1. Source: SGD

phospholipid biosynthetic process

Inferred from mutant phenotype Ref.1. Source: SGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 12871953. Source: SGD

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentendoplasmic reticulum

Inferred from direct assay Ref.9. Source: SGD

nuclear envelope

Inferred from direct assay Ref.9. Source: SGD

nuclear membrane

Inferred from direct assay Ref.8. Source: SGD

nucleoplasm

Inferred from direct assay Ref.8. Source: SGD

nucleus

Inferred from direct assay Ref.9Ref.8. Source: SGD

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 11454208PubMed 16429126. Source: IntAct

transcription corepressor activity

Inferred from physical interaction PubMed 11454208. Source: SGD

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SIN3P225795EBI-12555,EBI-17160

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 404404Transcriptional repressor OPI1
PRO_0000058061

Regions

Region109 – 13830Basic motif
Region139 – 16022Leucine-zipper
Motif200 – 2067FFAT
Compositional bias89 – 935Poly-Asp
Compositional bias193 – 1986Poly-Asp
Compositional bias286 – 29813Poly-Gln
Compositional bias306 – 3105Poly-Gln
Compositional bias377 – 3815Poly-Gln
Compositional bias384 – 3874Poly-Gln

Amino acid modifications

Modified residue101Phosphoserine Ref.10 Ref.11

Sequences

Sequence LengthMass (Da)Tools
P21957 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 59E55D7BE7122EDA

FASTA40446,065
        10         20         30         40         50         60 
MSENQRLGLS EEEVEAAEVL GVLKQSCRQK SQPSEDVSQA DKMPASESST TPLNILDRVS 

        70         80         90        100        110        120 
NKIISNVVTF YDEINTNKRP LKSIGRLLDD DDDEHDDYDY NDDEFFTNKR QKLSRAIAKG 

       130        140        150        160        170        180 
KDNLKEYKLN MSIESKKRLV TCLHLLKLAN KQLSDKISCL QDLVEKEQVH PLHKQDGNAR 

       190        200        210        220        230        240 
TTTGAGEDET SSDEDDDDEE FFDASEQVNA SEQSIVVKME VVGTVKKVYS LISKFTANSL 

       250        260        270        280        290        300 
PEPARSQVRE SLLNLPTNWF DSVHSTSLPH HASFHYANCE EQKVEQQQQQ QQQQQQQQLL 

       310        320        330        340        350        360 
QQQLLQQQQQ KRNKDGDDSA SPSSSVTANG KVLILAKESL EMVRNVMGVV DSTLGKAEEW 

       370        380        390        400 
VKQKQEVKEM IRERFLQQQQ QYRQQQQKDG NYVKPSQDNV DSKD 

« Hide

References

« Hide 'large scale' references
[1]"The OPI1 gene of Saccharomyces cerevisiae, a negative regulator of phospholipid biosynthesis, encodes a protein containing polyglutamine tracts and a leucine zipper."
White M.J., Hirsch J.P., Henry S.A.
J. Biol. Chem. 266:863-872(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII."
Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. expand/collapse author list , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M.
Science 265:2077-2082(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP."
Loewen C.J.R., Roy A., Levine T.P.
EMBO J. 22:2025-2035(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN FFAT MOTIF, INTERACTION WITH SCS2, SUBCELLULAR LOCATION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Gene recruitment of the activated INO1 locus to the nuclear membrane."
Brickner J.H., Walter P.
PLoS Biol. 2:1843-1852(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SCS2.
[9]"Phospholipid metabolism regulated by a transcription factor sensing phosphatidic acid."
Loewen C.J.R., Gaspar M.L., Jesch S.A., Delon C., Ktistakis N.T., Henry S.A., Levine T.P.
Science 304:1644-1647(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHATIDIC ACID-BINDING, SUBCELLULAR LOCATION.
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M57383 Genomic DNA. Translation: AAA34828.1.
U11582 Genomic DNA. Translation: AAB65073.1.
AY558103 Genomic DNA. Translation: AAS56429.1.
BK006934 Genomic DNA. Translation: DAA06665.1.
PIRS13741.
RefSeqNP_011843.1. NM_001179100.1.

3D structure databases

ProteinModelPortalP21957.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36403. 170 interactions.
DIPDIP-5505N.
IntActP21957. 8 interactions.
MINTMINT-491924.
STRING4932.YHL020C.

Proteomic databases

MaxQBP21957.
PaxDbP21957.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYHL020C; YHL020C; YHL020C.
GeneID856366.
KEGGsce:YHL020C.

Organism-specific databases

CYGDYHL020c.
SGDS000001012. OPI1.

Phylogenomic databases

eggNOGNOG46650.
KOK09062.
OMAHYANCEE.
OrthoDBEOG7CK3HM.

Enzyme and pathway databases

BioCycYEAST:G3O-31040-MONOMER.

Gene expression databases

GenevestigatorP21957.

Family and domain databases

InterProIPR013927. TF_Opi1.
[Graphical view]
PfamPF08618. Opi1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981834.

Entry information

Entry nameOPI1_YEAST
AccessionPrimary (citable) accession number: P21957
Secondary accession number(s): D3DKP9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VIII

Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD