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Protein

Transcriptional repressor OPI1

Gene

OPI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative regulator of the transcriptional complex INO2-INO4 in response to phospholipid precursor availability. When precursors become limiting, OPI1 is retained at the endoplasmic reticulum (ER) and INO2-INO4 activates INO1 and other genes required for phospholipid biosynthesis, whereas abundant precursor availability results in targeting of OPI1 to the nucleus to repress transcription of these genes. Binds directly to phosphatidic acid, which is required for ER targeting and may act as sensing mechanism for precursor availability, as phosphatidic acid becomes rapidly depleted upon phospholipid biosynthesis.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • phosphatidate phosphatase activity Source: GO_Central
  • phosphatidic acid binding Source: SGD
  • transcription coactivator activity Source: GO_Central
  • transcription corepressor activity Source: SGD

GO - Biological processi

  • endoplasmic reticulum unfolded protein response Source: SGD
  • fatty acid catabolic process Source: GO_Central
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • phospholipid biosynthetic process Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • triglyceride biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31040-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional repressor OPI1
Alternative name(s):
Negative regulator of phospholipid biosynthesis
Overproducer of inositol protein 1
Gene namesi
Name:OPI1
Ordered Locus Names:YHL020C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHL020C.
SGDiS000001012. OPI1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • nuclear envelope Source: SGD
  • nuclear membrane Source: SGD
  • nucleoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000580611 – 404Transcriptional repressor OPI1Add BLAST404

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21957.
PRIDEiP21957.

PTM databases

iPTMnetiP21957.

Interactioni

Subunit structurei

Interacts with SCS2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SIN3P225796EBI-12555,EBI-17160

Protein-protein interaction databases

BioGridi36403. 173 interactors.
DIPiDIP-5505N.
IntActiP21957. 8 interactors.
MINTiMINT-491924.

Structurei

3D structure databases

ProteinModelPortaliP21957.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 138Basic motifAdd BLAST30
Regioni139 – 160Leucine-zipperAdd BLAST22

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi200 – 206FFAT7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi89 – 93Poly-Asp5
Compositional biasi193 – 198Poly-Asp6
Compositional biasi286 – 298Poly-GlnAdd BLAST13
Compositional biasi306 – 310Poly-Gln5
Compositional biasi377 – 381Poly-Gln5
Compositional biasi384 – 387Poly-Gln4

Domaini

The FFAT motif is required for interaction with SCS2 and proper localization of the protein.1 Publication

Phylogenomic databases

InParanoidiP21957.
KOiK09062.
OMAiHYANCEE.
OrthoDBiEOG092C5A9Y.

Family and domain databases

InterProiIPR013927. TF_Opi1.
[Graphical view]
PfamiPF08618. Opi1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSENQRLGLS EEEVEAAEVL GVLKQSCRQK SQPSEDVSQA DKMPASESST
60 70 80 90 100
TPLNILDRVS NKIISNVVTF YDEINTNKRP LKSIGRLLDD DDDEHDDYDY
110 120 130 140 150
NDDEFFTNKR QKLSRAIAKG KDNLKEYKLN MSIESKKRLV TCLHLLKLAN
160 170 180 190 200
KQLSDKISCL QDLVEKEQVH PLHKQDGNAR TTTGAGEDET SSDEDDDDEE
210 220 230 240 250
FFDASEQVNA SEQSIVVKME VVGTVKKVYS LISKFTANSL PEPARSQVRE
260 270 280 290 300
SLLNLPTNWF DSVHSTSLPH HASFHYANCE EQKVEQQQQQ QQQQQQQQLL
310 320 330 340 350
QQQLLQQQQQ KRNKDGDDSA SPSSSVTANG KVLILAKESL EMVRNVMGVV
360 370 380 390 400
DSTLGKAEEW VKQKQEVKEM IRERFLQQQQ QYRQQQQKDG NYVKPSQDNV

DSKD
Length:404
Mass (Da):46,065
Last modified:August 1, 1991 - v1
Checksum:i59E55D7BE7122EDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57383 Genomic DNA. Translation: AAA34828.1.
U11582 Genomic DNA. Translation: AAB65073.1.
AY558103 Genomic DNA. Translation: AAS56429.1.
BK006934 Genomic DNA. Translation: DAA06665.1.
PIRiS13741.
RefSeqiNP_011843.1. NM_001179100.1.

Genome annotation databases

EnsemblFungiiYHL020C; YHL020C; YHL020C.
GeneIDi856366.
KEGGisce:YHL020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57383 Genomic DNA. Translation: AAA34828.1.
U11582 Genomic DNA. Translation: AAB65073.1.
AY558103 Genomic DNA. Translation: AAS56429.1.
BK006934 Genomic DNA. Translation: DAA06665.1.
PIRiS13741.
RefSeqiNP_011843.1. NM_001179100.1.

3D structure databases

ProteinModelPortaliP21957.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36403. 173 interactors.
DIPiDIP-5505N.
IntActiP21957. 8 interactors.
MINTiMINT-491924.

PTM databases

iPTMnetiP21957.

Proteomic databases

MaxQBiP21957.
PRIDEiP21957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHL020C; YHL020C; YHL020C.
GeneIDi856366.
KEGGisce:YHL020C.

Organism-specific databases

EuPathDBiFungiDB:YHL020C.
SGDiS000001012. OPI1.

Phylogenomic databases

InParanoidiP21957.
KOiK09062.
OMAiHYANCEE.
OrthoDBiEOG092C5A9Y.

Enzyme and pathway databases

BioCyciYEAST:G3O-31040-MONOMER.

Miscellaneous databases

PROiP21957.

Family and domain databases

InterProiIPR013927. TF_Opi1.
[Graphical view]
PfamiPF08618. Opi1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOPI1_YEAST
AccessioniPrimary (citable) accession number: P21957
Secondary accession number(s): D3DKP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1281 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.