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P21957

- OPI1_YEAST

UniProt

P21957 - OPI1_YEAST

Protein

Transcriptional repressor OPI1

Gene

OPI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Negative regulator of the transcriptional complex INO2-INO4 in response to phospholipid precursor availability. When precursors become limiting, OPI1 is retained at the endoplasmic reticulum (ER) and INO2-INO4 activates INO1 and other genes required for phospholipid biosynthesis, whereas abundant precursor availability results in targeting of OPI1 to the nucleus to repress transcription of these genes. Binds directly to phosphatidic acid, which is required for ER targeting and may act as sensing mechanism for precursor availability, as phosphatidic acid becomes rapidly depleted upon phospholipid biosynthesis.1 Publication

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. transcription corepressor activity Source: SGD

    GO - Biological processi

    1. endoplasmic reticulum unfolded protein response Source: SGD
    2. negative regulation of transcription from RNA polymerase II promoter Source: SGD
    3. phospholipid biosynthetic process Source: SGD
    4. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    5. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31040-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional repressor OPI1
    Alternative name(s):
    Negative regulator of phospholipid biosynthesis
    Overproducer of inositol protein 1
    Gene namesi
    Name:OPI1
    Ordered Locus Names:YHL020C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VIII

    Organism-specific databases

    CYGDiYHL020c.
    SGDiS000001012. OPI1.

    Subcellular locationi

    Endoplasmic reticulum. Nucleus
    Note: Maintained at the endoplasmic reticulum by SCS2. In response to elevated inositol levels.

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. nuclear envelope Source: SGD
    3. nuclear membrane Source: SGD
    4. nucleoplasm Source: SGD
    5. nucleus Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 404404Transcriptional repressor OPI1PRO_0000058061Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei10 – 101Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP21957.
    PaxDbiP21957.

    Expressioni

    Gene expression databases

    GenevestigatoriP21957.

    Interactioni

    Subunit structurei

    Interacts with SCS2.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SIN3P225795EBI-12555,EBI-17160

    Protein-protein interaction databases

    BioGridi36403. 170 interactions.
    DIPiDIP-5505N.
    IntActiP21957. 8 interactions.
    MINTiMINT-491924.
    STRINGi4932.YHL020C.

    Structurei

    3D structure databases

    ProteinModelPortaliP21957.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni109 – 13830Basic motifAdd
    BLAST
    Regioni139 – 16022Leucine-zipperAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi200 – 2067FFAT

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi89 – 935Poly-Asp
    Compositional biasi193 – 1986Poly-Asp
    Compositional biasi286 – 29813Poly-GlnAdd
    BLAST
    Compositional biasi306 – 3105Poly-Gln
    Compositional biasi377 – 3815Poly-Gln
    Compositional biasi384 – 3874Poly-Gln

    Domaini

    The FFAT motif is required for interaction with SCS2 and proper localization of the protein.1 Publication

    Phylogenomic databases

    eggNOGiNOG46650.
    KOiK09062.
    OMAiHYANCEE.
    OrthoDBiEOG7CK3HM.

    Family and domain databases

    InterProiIPR013927. TF_Opi1.
    [Graphical view]
    PfamiPF08618. Opi1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21957-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSENQRLGLS EEEVEAAEVL GVLKQSCRQK SQPSEDVSQA DKMPASESST    50
    TPLNILDRVS NKIISNVVTF YDEINTNKRP LKSIGRLLDD DDDEHDDYDY 100
    NDDEFFTNKR QKLSRAIAKG KDNLKEYKLN MSIESKKRLV TCLHLLKLAN 150
    KQLSDKISCL QDLVEKEQVH PLHKQDGNAR TTTGAGEDET SSDEDDDDEE 200
    FFDASEQVNA SEQSIVVKME VVGTVKKVYS LISKFTANSL PEPARSQVRE 250
    SLLNLPTNWF DSVHSTSLPH HASFHYANCE EQKVEQQQQQ QQQQQQQQLL 300
    QQQLLQQQQQ KRNKDGDDSA SPSSSVTANG KVLILAKESL EMVRNVMGVV 350
    DSTLGKAEEW VKQKQEVKEM IRERFLQQQQ QYRQQQQKDG NYVKPSQDNV 400
    DSKD 404
    Length:404
    Mass (Da):46,065
    Last modified:August 1, 1991 - v1
    Checksum:i59E55D7BE7122EDA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57383 Genomic DNA. Translation: AAA34828.1.
    U11582 Genomic DNA. Translation: AAB65073.1.
    AY558103 Genomic DNA. Translation: AAS56429.1.
    BK006934 Genomic DNA. Translation: DAA06665.1.
    PIRiS13741.
    RefSeqiNP_011843.1. NM_001179100.1.

    Genome annotation databases

    EnsemblFungiiYHL020C; YHL020C; YHL020C.
    GeneIDi856366.
    KEGGisce:YHL020C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57383 Genomic DNA. Translation: AAA34828.1 .
    U11582 Genomic DNA. Translation: AAB65073.1 .
    AY558103 Genomic DNA. Translation: AAS56429.1 .
    BK006934 Genomic DNA. Translation: DAA06665.1 .
    PIRi S13741.
    RefSeqi NP_011843.1. NM_001179100.1.

    3D structure databases

    ProteinModelPortali P21957.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36403. 170 interactions.
    DIPi DIP-5505N.
    IntActi P21957. 8 interactions.
    MINTi MINT-491924.
    STRINGi 4932.YHL020C.

    Proteomic databases

    MaxQBi P21957.
    PaxDbi P21957.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YHL020C ; YHL020C ; YHL020C .
    GeneIDi 856366.
    KEGGi sce:YHL020C.

    Organism-specific databases

    CYGDi YHL020c.
    SGDi S000001012. OPI1.

    Phylogenomic databases

    eggNOGi NOG46650.
    KOi K09062.
    OMAi HYANCEE.
    OrthoDBi EOG7CK3HM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31040-MONOMER.

    Miscellaneous databases

    NextBioi 981834.

    Gene expression databases

    Genevestigatori P21957.

    Family and domain databases

    InterProi IPR013927. TF_Opi1.
    [Graphical view ]
    Pfami PF08618. Opi1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The OPI1 gene of Saccharomyces cerevisiae, a negative regulator of phospholipid biosynthesis, encodes a protein containing polyglutamine tracts and a leucine zipper."
      White M.J., Hirsch J.P., Henry S.A.
      J. Biol. Chem. 266:863-872(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP."
      Loewen C.J.R., Roy A., Levine T.P.
      EMBO J. 22:2025-2035(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN FFAT MOTIF, INTERACTION WITH SCS2, SUBCELLULAR LOCATION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Gene recruitment of the activated INO1 locus to the nuclear membrane."
      Brickner J.H., Walter P.
      PLoS Biol. 2:1843-1852(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SCS2.
    9. "Phospholipid metabolism regulated by a transcription factor sensing phosphatidic acid."
      Loewen C.J.R., Gaspar M.L., Jesch S.A., Delon C., Ktistakis N.T., Henry S.A., Levine T.P.
      Science 304:1644-1647(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHATIDIC ACID-BINDING, SUBCELLULAR LOCATION.
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiOPI1_YEAST
    AccessioniPrimary (citable) accession number: P21957
    Secondary accession number(s): D3DKP9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1281 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome VIII
      Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

    External Data

    Dasty 3