Reviewed,
UniProtKB/Swiss-Prot P21957 (OPI1_YEAST)
Last modified
November 24, 2009.
Version 85.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Transcriptional repressor OPI1 Alternative name(s): Overproducer of inositol protein 1 Negative regulator of phospholipid biosynthesis | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 404 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Negative regulator of the transcriptional complex INO2-INO4 in response to phospholipid precursor availability. When precursors become limiting, OPI1 is retained at the endoplasmic reticulum (ER) and INO2-INO4 activates INO1 and other genes required for phospholipid biosynthesis, whereas abundant precursor availability results in targeting of OPI1 to the nucleus to repress transcription of these genes. Binds directly to phosphatidic acid, which is required for ER targeting and may act as sensing mechanism for precursor availability, as phosphatidic acid becomes rapidly depleted upon phospholipid biosynthesis. Ref.8 |
| Subunit structure | |
| Subcellular location | Endoplasmic reticulum. Nucleus. Note: Maintained at the endoplasmic reticulum by SCS2. In response to elevated inositol levels. Ref.8 Ref.4 Ref.5 |
| Domain | The FFAT motif is required for interaction with SCS2 and proper localization of the protein. Ref.4 |
| Miscellaneous | Present with 1281 molecules/cell in log phase SD medium. Ref.6 |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| INO2 | P26798 | 1 | EBI-12555,EBI-9262 | |
| SIN3 | P22579 | 3 | EBI-12555,EBI-17160 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 404 | 404 | Transcriptional repressor OPI1 | PRO_0000058061 | |||||
Regions | |||||||||
| Domain | 139 – 160 | 22 | Leucine-zipper | ||||||
| DNA binding | 109 – 138 | 30 | Basic motif | ||||||
| Motif | 200 – 206 | 7 | FFAT | ||||||
| Compositional bias | 89 – 93 | 5 | Poly-Asp | ||||||
| Compositional bias | 193 – 198 | 6 | Poly-Asp | ||||||
| Compositional bias | 286 – 298 | 13 | Poly-Gln | ||||||
| Compositional bias | 306 – 310 | 5 | Poly-Gln | ||||||
| Compositional bias | 377 – 381 | 5 | Poly-Gln | ||||||
| Compositional bias | 384 – 387 | 4 | Poly-Gln | ||||||
Amino acid modifications | |||||||||
| Modified residue | 10 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 49 | 1 | Phosphoserine Ref.11 | ||||||
| Modified residue | 230 | 1 | Phosphoserine Ref.11 Ref.10 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The OPI1 gene of Saccharomyces cerevisiae, a negative regulator of phospholipid biosynthesis, encodes a protein containing polyglutamine tracts and a leucine zipper." White M.J., Hirsch J.P., Henry S.A. J. Biol. Chem. 266:863-872(1991) [PubMed: 1985968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. Vaudin M.Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "A conserved ER targeting motif in three families of lipid binding proteins and in Opi1p binds VAP." Loewen C.J.R., Roy A., Levine T.P. EMBO J. 22:2025-2035(2003) [PubMed: 12727870] [Abstract] Cited for: DOMAIN FFAT MOTIF, INTERACTION WITH SCS2, SUBCELLULAR LOCATION. |
| [5] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [7] | "Gene recruitment of the activated INO1 locus to the nuclear membrane." Brickner J.H., Walter P. PLoS Biol. 2:1843-1852(2004) [PubMed: 15455074] [Abstract] Cited for: INTERACTION WITH SCS2. |
| [8] | "Phospholipid metabolism regulated by a transcription factor sensing phosphatidic acid." Loewen C.J.R., Gaspar M.L., Jesch S.A., Delon C., Ktistakis N.T., Henry S.A., Levine T.P. Science 304:1644-1647(2004) [PubMed: 15192221] [Abstract] Cited for: FUNCTION, PHOSPHATIDIC ACID-BINDING, SUBCELLULAR LOCATION. |
| [9] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, MASS SPECTROMETRY. |
| [10] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230, MASS SPECTROMETRY. |
| [11] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49 AND SER-230, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M57383 Genomic DNA. Translation: AAA34828.1. U11582 Genomic DNA. Translation: AAB65073.1. AY558103 Genomic DNA. Translation: AAS56429.1. | |
| PIR | S13741. |
| RefSeq | NP_011843.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:5505N. |
| IntAct | P21957. 13 interactions. |
| STRING | P21957. |
Genome annotation databases | |
| Ensembl | YHL020C; YHL020C; YHL020C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 856366. |
| KEGG | sce:YHL020C. |
| NMPDR | fig|4932.3.peg.2981. |
Organism-specific databases | |
| CYGD | YHL020c. |
| SGD | S000001012. OPI1. |
Phylogenomic databases | |
| HOGENOM | P21957. |
| OMA | KEMIRER |
| OrthoDB | EOG9F7Q2R |
Gene expression databases | |
| ArrayExpress | P21957. |
| Genevestigator | P21957. |
| GermOnline | YHL020C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR013927. TF_Opi1. [Graphical view] |
| Pfam | PF08618. Opi1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 981834. |
Entry information
| Entry name | OPI1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P21957 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VIII Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names |

Clusters with


