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Reviewed, UniProtKB/Swiss-Prot P21954 (IDHP_YEAST)

Last modified June 16, 2009. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Isocitrate dehydrogenase [NADP], mitochondrial
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP
Gene names
Name: IDP1
Ordered Locus Names: YDL066W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Mitochondrial IDP1 may regulate flux through the tricarboxylic acid cycle and respiration. Its probably critical function is the production of NADPH.

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Oxalosuccinate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Enzyme regulation

The enzyme is subject to end product inhibition by NADPH and 2-oxoglutarate.

Subunit structure

Homodimer.

Subcellular location

Mitochondrion.

Induction

By growth with glucose as a carbon source.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

KAR2P164741EBI-8898,EBI-7876

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1616Mitochondrion Ref.1
Chain17 – 428412Isocitrate dehydrogenase [NADP], mitochondrial
PRO_0000014426

Regions

Nucleotide binding91 – 933NADP By similarity
Nucleotide binding327 – 3326NADP By similarity
Region110 – 1167Substrate binding By similarity

Sites

Metal binding2691Magnesium or manganese By similarity
Metal binding2921Magnesium or manganese By similarity
Binding site931Substrate By similarity
Binding site981NADP By similarity
Binding site1251Substrate By similarity
Binding site1481Substrate By similarity
Binding site2771NADP By similarity
Binding site3451NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1551Critical for catalysis By similarity
Site2291Critical for catalysis By similarity

Secondary structure

1 ................................................................... 428
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P21954-1 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 7F885785B8682416

FASTA42848,190
        10         20         30         40         50         60 
MSMLSRRLFS TSRLAAFSKI KVKQPVVELD GDEMTRIIWD KIKKKLILPY LDVDLKYYDL 

        70         80         90        100        110        120 
SVESRDATSD KITQDAAEAI KKYGVGIKCA TITPDEARVK EFNLHKMWKS PNGTIRNILG 

       130        140        150        160        170        180 
GTVFREPIVI PRIPRLVPRW EKPIIIGRHA HGDQYKATDT LIPGPGSLEL VYKPSDPTTA 

       190        200        210        220        230        240 
QPQTLKVYDY KGSGVAMAMY NTDESIEGFA HSSFKLAIDK KLNLFLSTKN TILKKYDGRF 

       250        260        270        280        290        300 
KDIFQEVYEA QYKSKFEQLG IHYEHRLIDD MVAQMIKSKG GFIMALKNYD GDVQSDIVAQ 

       310        320        330        340        350        360 
GFGSLGLMTS ILVTPDGKTF ESEAAHGTVT RHYRKYQKGE ETSTNSIASI FAWSRGLLKR 

       370        380        390        400        410        420 
GELDNTPALC KFANILESAT LNTVQQDGIM TKDLALACGN NERSAYVTTE EFLDAVEKRL 


QKEIKSIE

« Hide

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References

« Hide 'large scale' references
[1]"Isolation, nucleotide sequence, and disruption of the Saccharomyces cerevisiae gene encoding mitochondrial NADP(H)-specific isocitrate dehydrogenase."
Haselbeck R.J., McAlister-Henn L.
J. Biol. Chem. 266:2339-2345(1991) [PubMed: 1989987] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 17-36.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M57229 Genomic DNA. Translation: AAA34703.1.
Z74114 Genomic DNA. Translation: CAA98631.1.
PIRDCBYIS. A38610.
RefSeqNP_010217.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2QFVX-ray2.30A/B/C/D16-428[»]
2QFWX-ray2.60A/B/C/D/E/F16-428[»]
2QFXX-ray2.70A/B/C/D/E/F16-428[»]
2QFYX-ray2.10A/B/C/D/E/F16-428[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:4494N.
IntActP21954. 15 interactions.

Proteomic databases

PeptideAtlasP21954.

Genome annotation databases

EnsemblYDL066W. Saccharomyces cerevisiae. [Contig view]
GeneID851493.
GenomeReviewsGene locus YDL066W in contig Z71256_GR.
KEGGsce:YDL066W.
NMPDRfig|4932.3.peg.957.

Organism-specific databases

CYGDYDL066w.
SGDS000002224. IDP1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP21954.
OMAP21954. ATAYEKK.

Enzyme and pathway databases

BRENDA1.1.1.42. 250.

Gene expression databases

ArrayExpressP21954.
GermOnlineYDL066W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR004790. Isocitrate_DH_NADP-dep_euk.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHERPTHR11822. IDH_NADP_euk. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio968825.

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Entry information

Entry nameIDHP_YEAST
AccessionPrimary (citable) accession number: P21954
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: June 16, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents