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Protein

POU domain, class 3, transcription factor 1

Gene

Pou3f1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi337 – 39660HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • epidermis development Source: MGI
  • forebrain development Source: MGI
  • keratinocyte differentiation Source: MGI
  • myelination Source: MGI
  • myelination in peripheral nervous system Source: MGI
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • Schwann cell development Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 1
Alternative name(s):
Octamer-binding protein 6
Short name:
Oct-6
Octamer-binding transcription factor 6
Short name:
OTF-6
POU domain transcription factor SCIP
Gene namesi
Name:Pou3f1
Synonyms:Oct6, Otf-6, Otf6, Scip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:101896. Pou3f1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449POU domain, class 3, transcription factor 1PRO_0000100721Add
BLAST

Proteomic databases

MaxQBiP21952.
PRIDEiP21952.

Expressioni

Tissue specificityi

Expressed in embryonal stem cells and in the developing brain.

Gene expression databases

BgeeiP21952.
CleanExiMM_POU3F1.
ExpressionAtlasiP21952. baseline and differential.
GenevisibleiP21952. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000137374.

Structurei

Secondary structure

1
449
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi250 – 26617Combined sources
Helixi271 – 28212Combined sources
Helixi288 – 2958Combined sources
Helixi301 – 31818Combined sources
Helixi346 – 35813Combined sources
Helixi364 – 37411Combined sources
Helixi378 – 39215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XSDX-ray2.05C240-402[»]
ProteinModelPortaliP21952.
SMRiP21952. Positions 247-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini245 – 31975POU-specificPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG261729.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP21952.
KOiK09365.
OMAiHPQWLPT.
OrthoDBiEOG7DJSMG.
PhylomeDBiP21952.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21952-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAERLHA GAAYREVQKL
60 70 80 90 100
MHHEWLGAGA GHPVGLAHPQ WLPTGGGGGG DWAGGPHLEH GKAGGGGTGR
110 120 130 140 150
ADDGGGGGGF HARLVHQGAA HAGAAWAQGG TAHHLGPAMS PSPGAGGGHQ
160 170 180 190 200
PQPLGLYAQA AYPGGGGGGL AGMLAAGGGG AGPGLHHALH EDGHEAQLEP
210 220 230 240 250
SPPPHLGAHG HAHGHAHAGG LHAAAAHLHP GAGGGGSSVG EHSDEDAPSS
260 270 280 290 300
DDLEQFAKQF KQRRIKLGFT QADVGLALGT LYGNVFSQTT ICRFEALQLS
310 320 330 340 350
FKNMCKLKPL LNKWLEETDS SSGSPTNLDK IAAQGRKRKK RTSIEVGVKG
360 370 380 390 400
ALESHFLKCP KPSAHEITGL ADSLQLEKEV VRVWFCNRRQ KEKRMTPAAG
410 420 430 440
AGHPPMDDVY APGELGPGGG SASPPSAPPP PPPAALHHHH HHTLPGSVQ
Length:449
Mass (Da):45,324
Last modified:August 1, 1991 - v1
Checksum:i82DD1335A0C4F43C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351Missing in CAA40720 (PubMed:1976514).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54628 mRNA. Translation: CAA38445.1.
X56959 mRNA. Translation: CAA40280.1.
X57482 mRNA. Translation: CAA40720.1.
M88302 Genomic DNA. Translation: AAA39963.1.
CCDSiCCDS57296.1.
PIRiS30205.
RefSeqiNP_035271.1. NM_011141.2.
UniGeneiMm.297371.

Genome annotation databases

EnsembliENSMUST00000053491; ENSMUSP00000137374; ENSMUSG00000090125.
GeneIDi18991.
KEGGimmu:18991.
UCSCiuc029uzh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54628 mRNA. Translation: CAA38445.1.
X56959 mRNA. Translation: CAA40280.1.
X57482 mRNA. Translation: CAA40720.1.
M88302 Genomic DNA. Translation: AAA39963.1.
CCDSiCCDS57296.1.
PIRiS30205.
RefSeqiNP_035271.1. NM_011141.2.
UniGeneiMm.297371.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XSDX-ray2.05C240-402[»]
ProteinModelPortaliP21952.
SMRiP21952. Positions 247-397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000137374.

Proteomic databases

MaxQBiP21952.
PRIDEiP21952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053491; ENSMUSP00000137374; ENSMUSG00000090125.
GeneIDi18991.
KEGGimmu:18991.
UCSCiuc029uzh.1. mouse.

Organism-specific databases

CTDi5453.
MGIiMGI:101896. Pou3f1.

Phylogenomic databases

eggNOGiNOG261729.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP21952.
KOiK09365.
OMAiHPQWLPT.
OrthoDBiEOG7DJSMG.
PhylomeDBiP21952.
TreeFamiTF316413.

Miscellaneous databases

NextBioi295382.
PROiP21952.
SOURCEiSearch...

Gene expression databases

BgeeiP21952.
CleanExiMM_POU3F1.
ExpressionAtlasiP21952. baseline and differential.
GenevisibleiP21952. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. TF_POU_3.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The octamer binding factor Oct6: cDNA cloning and expression in early embryonic cells."
    Meijer D., Graus A., Kraay R., Langeveld A., Mulder M.P., Grosveld G.
    Nucleic Acids Res. 18:7357-7365(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Brain.
  2. "Oct-6: a POU transcription factor expressed in embryonal stem cells and in the developing brain."
    Suzuki N., Rohdewohld H., Neuman T., Gruss P., Schoeler H.R.
    EMBO J. 9:3723-3732(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "Nucleotide sequence of mouse SCIP cDNA, a POU-domain transcription factor."
    Zimmerman E.C., Jones C.M., Fet V., Hogan B.L.M., Magnuson M.A.
    Nucleic Acids Res. 19:956-956(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  4. "Structure and evolution of four POU domain genes expressed in mouse brain."
    Hara Y., Rovescalli C., Kim Y., Nirenberg M.
    Proc. Natl. Acad. Sci. U.S.A. 89:3280-3284(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPO3F1_MOUSE
AccessioniPrimary (citable) accession number: P21952
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 22, 2015
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.