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P21952 (PO3F1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 3, transcription factor 1
Alternative name(s):
Octamer-binding protein 6
Short name=Oct-6
Octamer-binding transcription factor 6
Short name=OTF-6
POU domain transcription factor SCIP
Gene names
Name:Pou3f1
Synonyms:Oct6, Otf-6, Otf6, Scip
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.

Subcellular location

Nucleus.

Tissue specificity

Expressed in embryonal stem cells and in the developing brain.

Sequence similarities

Belongs to the POU transcription factor family. Class-3 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processSchwann cell development

Inferred from mutant phenotype PubMed 12782656. Source: MGI

epidermis development

Inferred from genetic interaction PubMed 9242494. Source: MGI

forebrain development

Inferred from mutant phenotype PubMed 19527706. Source: MGI

keratinocyte differentiation

Inferred from genetic interaction PubMed 9242494. Source: MGI

myelination

Inferred from mutant phenotype PubMed 15282162. Source: MGI

myelination in peripheral nervous system

Inferred from genetic interaction PubMed 12782656. Source: MGI

positive regulation of gene expression

Inferred from direct assay PubMed 15713637. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence orthology PubMed 9632656. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 15713637. Source: UniProtKB

transcription from RNA polymerase II promoter

Inferred from sequence orthology PubMed 9632656. Source: GOC

   Cellular_componentnucleus

Inferred from direct assay PubMed 15713637. Source: UniProtKB

transcription factor complex

Inferred from direct assay PubMed 12782656. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 12782656. Source: MGI

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from sequence orthology PubMed 9632656. Source: MGI

RNA polymerase II transcription coactivator activity

Inferred from direct assay PubMed 18505825. Source: UniProtKB

sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449POU domain, class 3, transcription factor 1
PRO_0000100721

Regions

Domain245 – 31975POU-specific
DNA binding337 – 39660Homeobox

Experimental info

Sequence conflict351Missing in CAA40720. Ref.2

Secondary structure

............... 449
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P21952 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 82DD1335A0C4F43C

FASTA44945,324
        10         20         30         40         50         60 
MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAERLHA GAAYREVQKL MHHEWLGAGA 

        70         80         90        100        110        120 
GHPVGLAHPQ WLPTGGGGGG DWAGGPHLEH GKAGGGGTGR ADDGGGGGGF HARLVHQGAA 

       130        140        150        160        170        180 
HAGAAWAQGG TAHHLGPAMS PSPGAGGGHQ PQPLGLYAQA AYPGGGGGGL AGMLAAGGGG 

       190        200        210        220        230        240 
AGPGLHHALH EDGHEAQLEP SPPPHLGAHG HAHGHAHAGG LHAAAAHLHP GAGGGGSSVG 

       250        260        270        280        290        300 
EHSDEDAPSS DDLEQFAKQF KQRRIKLGFT QADVGLALGT LYGNVFSQTT ICRFEALQLS 

       310        320        330        340        350        360 
FKNMCKLKPL LNKWLEETDS SSGSPTNLDK IAAQGRKRKK RTSIEVGVKG ALESHFLKCP 

       370        380        390        400        410        420 
KPSAHEITGL ADSLQLEKEV VRVWFCNRRQ KEKRMTPAAG AGHPPMDDVY APGELGPGGG 

       430        440 
SASPPSAPPP PPPAALHHHH HHTLPGSVQ 

« Hide

References

[1]"The octamer binding factor Oct6: cDNA cloning and expression in early embryonic cells."
Meijer D., Graus A., Kraay R., Langeveld A., Mulder M.P., Grosveld G.
Nucleic Acids Res. 18:7357-7365(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X CBA.
Tissue: Brain.
[2]"Oct-6: a POU transcription factor expressed in embryonal stem cells and in the developing brain."
Suzuki N., Rohdewohld H., Neuman T., Gruss P., Schoeler H.R.
EMBO J. 9:3723-3732(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Nucleotide sequence of mouse SCIP cDNA, a POU-domain transcription factor."
Zimmerman E.C., Jones C.M., Fet V., Hogan B.L.M., Magnuson M.A.
Nucleic Acids Res. 19:956-956(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[4]"Structure and evolution of four POU domain genes expressed in mouse brain."
Hara Y., Rovescalli C., Kim Y., Nirenberg M.
Proc. Natl. Acad. Sci. U.S.A. 89:3280-3284(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54628 mRNA. Translation: CAA38445.1.
X56959 mRNA. Translation: CAA40280.1.
X57482 mRNA. Translation: CAA40720.1.
M88302 Genomic DNA. Translation: AAA39963.1.
CCDSCCDS57296.1.
PIRS30205.
RefSeqNP_035271.1. NM_011141.2.
UniGeneMm.297371.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2XSDX-ray2.05C240-402[»]
ProteinModelPortalP21952.
SMRP21952. Positions 247-397.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000100650.

Proteomic databases

PRIDEP21952.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000053491; ENSMUSP00000137374; ENSMUSG00000090125.
GeneID18991.
KEGGmmu:18991.
UCSCuc029uzh.1. mouse.

Organism-specific databases

CTD5453.
MGIMGI:101896. Pou3f1.

Phylogenomic databases

eggNOGNOG261729.
GeneTreeENSGT00730000110809.
HOGENOMHOG000116303.
HOVERGENHBG053120.
InParanoidP21952.
KOK09365.
OMAKLMHHEW.
OrthoDBEOG7DJSMG.
PhylomeDBP21952.
TreeFamTF316413.

Gene expression databases

BgeeP21952.
CleanExMM_POU3F1.
GenevestigatorP21952.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295382.
PROP21952.
SOURCESearch...

Entry information

Entry namePO3F1_MOUSE
AccessionPrimary (citable) accession number: P21952
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 9, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot