Reviewed, UniProtKB/Swiss-Prot P21949 (MBHL_AZOVI)

Names and origin

Protein names

Recommended name:
    Uptake hydrogenase large subunit
    EC=1.12.99.6
Alternative name(s):
    Hydrogenlyase
    Membrane-bound hydrogenase large subunit
    Hydrogenase subunit alpha

Gene names

Name:

hoxG

Organism

Azotobacter vinelandii

Taxonomic identifier

354 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme recycles the H2 produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O2 under carbon- or phosphate-limited conditions.
Catalytic activity	H2 + A = AH2.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Subunit structure	Heterodimer of a large and a small subunit.
Subcellular location	Cell membrane; Peripheral membrane protein.
Post-translational modification	15 or 16 residues are removed from the C-terminus during maturation of the protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	Metal-binding Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro hydrogenase (acceptor) activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	Uptake hydrogenase large subunit		PRO_0000199709
Sites
	Metal binding	74	1	Nickel Potential
	Metal binding	77	1	Nickel Potential
	Metal binding	581	1	Nickel Potential
	Metal binding	584	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	P21949-1 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: BCC64BB2F12B3240	FASTA	602	66,737
	10 20 30 40 50 60 MSSLPNASQL DKSGRRIVVD PVTRIEGHMR CEVNVDASNV ITNAVSTGTM WRGLEVILKG 70 80 90 100 110 120 RDPRDAWAFV ERICGVCTGT HALTSVRAVE DALDIRIPYN AHLIRNLMDK TLQVHDHIVH 130 140 150 160 170 180 FYHLHALDWV NPVNALKADP KATSALQQAV SPAHAKSSPG YFRDVQTRLK KFVESGQLGL 190 200 210 220 230 240 FSNGYWDNPA YKLPPEADLM AVAHYLEALD LQKDIVKIHT IFGGKNPHPN YMVGGVACAI 250 260 270 280 290 300 NLDDVGAAGA PVNMTSLNFV LERIHEAREF TRNVYLPDVL AVAGIYKDWL YGGGLAAHNL 310 320 330 340 350 360 LSYGTFTKVP YDKSSDLLPA GAIVGGNWDE VLPVDVRDPE EIQEFVSHSW YSYADETKGL 370 380 390 400 410 420 HPWDGVTEPK FELGPNTKGS RTHIQEIDEA HKYSWIKAPR WRGHAMEVGP LARYIIAYAS 430 440 450 460 470 480 GREYVKEQVD RSLAAFNQST GLNLGLKQFL PSTLGRTLAR ALECELAVDS MLDDWQALVG 490 500 510 520 530 540 NIKAGDRATA NVEKWDPSTW PKEAKGVGIN EAPRGALGHW IRIKDGKIEN YQAIVPTTWN 550 560 570 580 590 600 GTPRDHLGNI GAYEAALLNT RMERPDEPVE ILRTLHSFDP CLACSTHVMS PDGQELTRVK VR

References

[1]

"Cloning, sequencing and characterization of the [NiFe]hydrogenase-encoding structural genes (hoxK and hoxG) from Azotobacter vinelandii." Menon A.L., Stults L.W., Robson R.L., Mortenson L.E. Gene 96:67-74(1990) [PubMed: 2265761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M33152 Genomic DNA. Translation: AAA82506.1. L23970 Unassigned DNA. Translation: AAA19499.1.
PIR	JQ0806.
3D structure databases
HSSP	HSSP built from PDB template 2FRV based on UniProtKB P12944.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.99.6. 883.
Family and domain databases
InterPro	IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
Pfam	PF00374. NiFeSe_Hases. 1 hit. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MBHL_AZOVI

Accession

Primary (citable) accession number: P21949

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)