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Protein

Alkaline phosphatase

Gene

phoA

Organism
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg2+ ion.
  • Zn2+Note: Binds 2 Zn2+ ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi74MagnesiumBy similarity1
Metal bindingi74Zinc 2By similarity1
Active sitei125Phosphoserine intermediatePROSITE-ProRule annotation1
Metal bindingi176MagnesiumBy similarity1
Metal bindingi178MagnesiumBy similarity1
Metal bindingi345MagnesiumBy similarity1
Metal bindingi350Zinc 1By similarity1
Metal bindingi354Zinc 1By similarity1
Metal bindingi392Zinc 2By similarity1
Metal bindingi393Zinc 2By similarity1
Metal bindingi435Zinc 1By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Short name:
APase
Gene namesi
Name:phoA
Ordered Locus Names:EFER_2640
OrganismiEscherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Taxonomic identifieri585054 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000745 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000002401322 – 472Alkaline phosphataseAdd BLAST451

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi191 ↔ 201By similarity
Disulfide bondi309 ↔ 359By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP21948.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000099117.
KOiK01077.
OMAiDITAWEY.
OrthoDBiPOG091H0B8M.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQSAIALAL LSCLITPVSQ AQTSQNINIL ENRAAQGDIT MPGGARRLSG
60 70 80 90 100
DQTEALRASL NDKPAKNIIL LIGDGMGDSE ITAARNYAEG AGGYFKGIDA
110 120 130 140 150
LPLTGQYTHY ALDKKTGKPD YVTDSAASAT AWTTGVKTYN GALGVDIHEN
160 170 180 190 200
PHTTILEMAK AAGLATGNVS TAELQDATPA ALVSHVTSRK CYGPSVTSEK
210 220 230 240 250
CPGNALEKGG KGSITEQLLN ARADVTLGGG AKTFAETATA GEWQGKTLRE
260 270 280 290 300
QALARGYQIV SDAASLAAVT QAGQDKPLLG LFAEGNMPVR WHGPKASYHG
310 320 330 340 350
NLDKPAVTCT PNPQRNETVP TLAQMTDKAI ELLSKNERGF FLQVEGASID
360 370 380 390 400
KQDHAANPCG QIGETVDLDE AVQRALEFAK KDGNTLVIVT ADHAHSSQIV
410 420 430 440 450
APDTKAPGLT QALNTKDGAV MAISYGNSEE DSQEHTGSQL RIAAYGPNAA
460 470
NVVGLTDQTD LFYTMKAALG LQ
Length:472
Mass (Da):49,430
Last modified:May 1, 1991 - v1
Checksum:iEF956C964F931141
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33966 Genomic DNA. Translation: AAA24371.1.
CU928158 Genomic DNA. Translation: CAQ90135.1.
PIRiI57445.
RefSeqiWP_000813782.1. NC_011740.1.

Genome annotation databases

EnsemblBacteriaiCAQ90135; CAQ90135; EFER_2640.
KEGGiefe:EFER_2640.
PATRICi32129652. VBIEscFer122920_2555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33966 Genomic DNA. Translation: AAA24371.1.
CU928158 Genomic DNA. Translation: CAQ90135.1.
PIRiI57445.
RefSeqiWP_000813782.1. NC_011740.1.

3D structure databases

ProteinModelPortaliP21948.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ90135; CAQ90135; EFER_2640.
KEGGiefe:EFER_2640.
PATRICi32129652. VBIEscFer122920_2555.

Phylogenomic databases

HOGENOMiHOG000099117.
KOiK01077.
OMAiDITAWEY.
OrthoDBiPOG091H0B8M.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPB_ESCF3
AccessioniPrimary (citable) accession number: P21948
Secondary accession number(s): B7LMK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.