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P21948 (PPB_ESCF3) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkaline phosphatase

Short name=APase
EC=3.1.3.1
Gene names
Name:phoA
Ordered Locus Names:EFER_2640
OrganismEscherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) [Complete proteome] [HAMAP]
Taxonomic identifier585054 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion.

Binds 2 zinc ions.

Subunit structure

Homodimer.

Subcellular location

Periplasm.

Sequence similarities

Belongs to the alkaline phosphatase family.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainSignal
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMDisulfide bond
Phosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalkaline phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121
Chain22 – 472451Alkaline phosphatase
PRO_0000024013

Sites

Active site1251Phosphoserine intermediate By similarity
Metal binding741Magnesium By similarity
Metal binding741Zinc 2 By similarity
Metal binding1761Magnesium By similarity
Metal binding1781Magnesium By similarity
Metal binding3451Magnesium By similarity
Metal binding3501Zinc 1 By similarity
Metal binding3541Zinc 1 By similarity
Metal binding3921Zinc 2 By similarity
Metal binding3931Zinc 2 By similarity
Metal binding4351Zinc 1 By similarity

Amino acid modifications

Disulfide bond191 ↔ 201 By similarity
Disulfide bond309 ↔ 359 By similarity

Sequences

Sequence LengthMass (Da)Tools
P21948 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: EF956C964F931141

FASTA47249,430
        10         20         30         40         50         60 
MKQSAIALAL LSCLITPVSQ AQTSQNINIL ENRAAQGDIT MPGGARRLSG DQTEALRASL 

        70         80         90        100        110        120 
NDKPAKNIIL LIGDGMGDSE ITAARNYAEG AGGYFKGIDA LPLTGQYTHY ALDKKTGKPD 

       130        140        150        160        170        180 
YVTDSAASAT AWTTGVKTYN GALGVDIHEN PHTTILEMAK AAGLATGNVS TAELQDATPA 

       190        200        210        220        230        240 
ALVSHVTSRK CYGPSVTSEK CPGNALEKGG KGSITEQLLN ARADVTLGGG AKTFAETATA 

       250        260        270        280        290        300 
GEWQGKTLRE QALARGYQIV SDAASLAAVT QAGQDKPLLG LFAEGNMPVR WHGPKASYHG 

       310        320        330        340        350        360 
NLDKPAVTCT PNPQRNETVP TLAQMTDKAI ELLSKNERGF FLQVEGASID KQDHAANPCG 

       370        380        390        400        410        420 
QIGETVDLDE AVQRALEFAK KDGNTLVIVT ADHAHSSQIV APDTKAPGLT QALNTKDGAV 

       430        440        450        460        470 
MAISYGNSEE DSQEHTGSQL RIAAYGPNAA NVVGLTDQTD LFYTMKAALG LQ 

« Hide

References

« Hide 'large scale' references
[1]"The molecular evolution of bacterial alkaline phosphatase: correlating variation among enteric bacteria to experimental manipulations of the protein."
Dubose R.F., Hartl D.L.
Mol. Biol. Evol. 7:547-577(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths."
Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. expand/collapse author list , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E.
PLoS Genet. 5:E1000344-E1000344(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35469 / DSM 13698 / CDC 0568-73.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33966 Genomic DNA. Translation: AAA24371.1.
CU928158 Genomic DNA. Translation: CAQ90135.1.
PIRI57445.
RefSeqYP_002383746.1. NC_011740.1.

3D structure databases

ProteinModelPortalP21948.
SMRP21948. Positions 29-472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING585054.EFER_2640.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAQ90135; CAQ90135; EFER_2640.
GeneID7124018.
KEGGefe:EFER_2640.
PATRIC32129652. VBIEscFer122920_2555.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1785.
HOGENOMHOG000099117.
KOK01077.
OMAAGHERNI.
OrthoDBEOG661H4G.

Enzyme and pathway databases

BioCycEFER585054:GJJM-2643-MONOMER.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. SSF53649. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPB_ESCF3
AccessionPrimary (citable) accession number: P21948
Secondary accession number(s): B7LMK2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families