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Protein

Alkaline phosphatase

Gene

phoA

Organism
Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg2+ ion.
  • Zn2+Note: Binds 2 Zn2+ ions.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi74 – 741MagnesiumBy similarity
Metal bindingi74 – 741Zinc 2By similarity
Active sitei125 – 1251Phosphoserine intermediatePROSITE-ProRule annotation
Metal bindingi176 – 1761MagnesiumBy similarity
Metal bindingi178 – 1781MagnesiumBy similarity
Metal bindingi345 – 3451MagnesiumBy similarity
Metal bindingi350 – 3501Zinc 1By similarity
Metal bindingi354 – 3541Zinc 1By similarity
Metal bindingi392 – 3921Zinc 2By similarity
Metal bindingi393 – 3931Zinc 2By similarity
Metal bindingi435 – 4351Zinc 1By similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEFER585054:GJJM-2643-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Short name:
APase
Gene namesi
Name:phoA
Ordered Locus Names:EFER_2640
OrganismiEscherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
Taxonomic identifieri585054 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000745 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 472451Alkaline phosphatasePRO_0000024013Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi191 ↔ 201By similarity
Disulfide bondi309 ↔ 359By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi585054.EFER_2640.

Structurei

3D structure databases

ProteinModelPortaliP21948.
SMRiP21948. Positions 29-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099117.
KOiK01077.
OMAiCPSNALE.
OrthoDBiEOG661H4G.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQSAIALAL LSCLITPVSQ AQTSQNINIL ENRAAQGDIT MPGGARRLSG
60 70 80 90 100
DQTEALRASL NDKPAKNIIL LIGDGMGDSE ITAARNYAEG AGGYFKGIDA
110 120 130 140 150
LPLTGQYTHY ALDKKTGKPD YVTDSAASAT AWTTGVKTYN GALGVDIHEN
160 170 180 190 200
PHTTILEMAK AAGLATGNVS TAELQDATPA ALVSHVTSRK CYGPSVTSEK
210 220 230 240 250
CPGNALEKGG KGSITEQLLN ARADVTLGGG AKTFAETATA GEWQGKTLRE
260 270 280 290 300
QALARGYQIV SDAASLAAVT QAGQDKPLLG LFAEGNMPVR WHGPKASYHG
310 320 330 340 350
NLDKPAVTCT PNPQRNETVP TLAQMTDKAI ELLSKNERGF FLQVEGASID
360 370 380 390 400
KQDHAANPCG QIGETVDLDE AVQRALEFAK KDGNTLVIVT ADHAHSSQIV
410 420 430 440 450
APDTKAPGLT QALNTKDGAV MAISYGNSEE DSQEHTGSQL RIAAYGPNAA
460 470
NVVGLTDQTD LFYTMKAALG LQ
Length:472
Mass (Da):49,430
Last modified:May 1, 1991 - v1
Checksum:iEF956C964F931141
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33966 Genomic DNA. Translation: AAA24371.1.
CU928158 Genomic DNA. Translation: CAQ90135.1.
PIRiI57445.
RefSeqiWP_000813782.1. NC_011740.1.
YP_002383746.1. NC_011740.1.

Genome annotation databases

EnsemblBacteriaiCAQ90135; CAQ90135; EFER_2640.
GeneIDi7124018.
KEGGiefe:EFER_2640.
PATRICi32129652. VBIEscFer122920_2555.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33966 Genomic DNA. Translation: AAA24371.1.
CU928158 Genomic DNA. Translation: CAQ90135.1.
PIRiI57445.
RefSeqiWP_000813782.1. NC_011740.1.
YP_002383746.1. NC_011740.1.

3D structure databases

ProteinModelPortaliP21948.
SMRiP21948. Positions 29-472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi585054.EFER_2640.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAQ90135; CAQ90135; EFER_2640.
GeneIDi7124018.
KEGGiefe:EFER_2640.
PATRICi32129652. VBIEscFer122920_2555.

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099117.
KOiK01077.
OMAiCPSNALE.
OrthoDBiEOG661H4G.

Enzyme and pathway databases

BioCyciEFER585054:GJJM-2643-MONOMER.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The molecular evolution of bacterial alkaline phosphatase: correlating variation among enteric bacteria to experimental manipulations of the protein."
    Dubose R.F., Hartl D.L.
    Mol. Biol. Evol. 7:547-577(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35469 / DSM 13698 / CDC 0568-73.

Entry informationi

Entry nameiPPB_ESCF3
AccessioniPrimary (citable) accession number: P21948
Secondary accession number(s): B7LMK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: January 7, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.