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Protein

Nuclear shuttle protein

Gene

BR1

Organism
Squash leaf curl virus (SLCV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the genomic viral ssDNA, shuttles it into and out of the cell nucleus. Begomoviruses use 2 proteins to transport their DNA from cell to cell. The nuclear shuttle protein (NSP) shuttles it between nucleus and cytoplasm and the movement protein (MP) probably transports the DNA-NSP complex to the cell periphery and facilitates movement across the cell wall.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Viral movement protein
Biological processHost-virus interaction, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear shuttle protein
Short name:
NSP
Alternative name(s):
Protein BR1
Protein BV1
Gene namesi
ORF Names:BR1, BV1
OrganismiSquash leaf curl virus (SLCV)
Taxonomic identifieri10829 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeBegomovirus
Virus hostiCucurbita moschata (Winter crookneck squash) (Cucurbita pepo var. moschata) [TaxID: 3662]
Cucurbita pepo (Vegetable marrow) (Summer squash) [TaxID: 3663]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]

Subcellular locationi

  • Host nucleus 1 Publication
  • Host cytoplasm 1 Publication
  • Host cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

  • Note: Translocated to the plasma membrane by the movement protein BC1.

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host cytoplasm, Host membrane, Host nucleus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi25 – 26KR → AA: Delayed nuclear targeting. No effect on infectivity. 1 Publication2
Mutagenesisi36 – 38RRR → AAA: No effect on subcellular location. 1 Publication3
Mutagenesisi89 – 91RTR → ATA: Mislocalizes to the cytoplasm. Complete loss of infectivity. 1 Publication3
Mutagenesisi97 – 100KRVR → AAVA: No effect on subcellular location. 1 Publication4
Mutagenesisi148 – 149FD → AA: No effect on subcellular location. 1 Publication2
Mutagenesisi170 – 171DR → AA: No effect on subcellular location. 1 Publication2
Mutagenesisi189 – 193LLIDL → AAIDA: Complete loss of infectivity. 1 Publication5
Mutagenesisi201 – 203NKR → AAA: Delayed nuclear targeting. No effect on infectivity. 1 Publication3
Mutagenesisi212D → A: Delayed nuclear targeting. 1 Publication1
Mutagenesisi215 – 216RD → AA: Delayed nuclear targeting. 1 Publication2
Mutagenesisi224N → A: Mislocalizes to the cytoplasm. Complete loss of infectivity. 1 Publication1
Mutagenesisi227 – 228KN → AA: Mislocalizes to the cytoplasm. Complete loss of infectivity. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002222671 – 256Nuclear shuttle proteinAdd BLAST256

Interactioni

Subunit structurei

Binds to single-stranded and double-stranded viral DNA. Interacts with the host nuclear shuttle interacting (NSI) protein. This interaction may allow NSP to recruit NSI monomers to the viral genome and thus regulate nuclear export of viral genome by NSP (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 187Interaction with Arabidopsis thaliana NSI proteinBy similarityAdd BLAST38

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 42Bipartite nuclear localization signalAdd BLAST22
Motifi81 – 96Nuclear localization signal1 PublicationAdd BLAST16
Motifi177 – 198Nuclear export signalSequence analysisAdd BLAST22

Sequence similaritiesi

Phylogenomic databases

KOiK21235.

Family and domain databases

InterProiView protein in InterPro
IPR001530. Gemini_BR1.
IPR000263. GV_A/BR1_coat.
PfamiView protein in Pfam
PF00844. Gemini_coat. 1 hit.
PRINTSiPR00223. GEMCOATARBR1.
PR00225. GEMCOATBR1.

Sequencei

Sequence statusi: Complete.

P21935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSTSNRRGR SQTQRGSHVR RTGVKRSYGA ARGDDRRRPN VVSKTQVEPR
60 70 80 90 100
MTIQRVQENQ FGPEFVLSQN SALSTFVTYP SYVKTVPNRT RTYIKLKRVR
110 120 130 140 150
FKGTLKIERG QGDTIMDGPS SNIEGVFSMV IVVDRKPHVS QSGRLHTFDE
160 170 180 190 200
LFGARIHCHG NLSVVPALKD RYYIRHVTKR VVSLEKDTLL IDLHGTTQLS
210 220 230 240 250
NKRYNCWASF SDLERDSCNG VYGNITKNAL LVYYCWLSDA QSKASTYVSF

ELDYLG
Length:256
Mass (Da):29,228
Last modified:March 18, 2008 - v2
Checksum:i0EC879D1983DEFAF
GO

Sequence cautioni

The sequence AAC32408 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38182 Genomic DNA. Translation: AAC32408.1. Different initiation.
PIRiA36785. QQCVSV.
RefSeqiNP_047247.2. NC_001937.1.

Genome annotation databases

GeneIDi956398.
KEGGivg:956398.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNSP_SLCV
AccessioniPrimary (citable) accession number: P21935
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: March 18, 2008
Last modified: May 10, 2017
This is version 70 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families