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Protein

Cholinesterase

Gene

BCHE

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters (By similarity).By similarity

Catalytic activityi

An acylcholine + H2O = choline + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei205 – 2051Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei332 – 3321Charge relay systemBy similarity
Active sitei445 – 4451Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Protein family/group databases

MEROPSiS09.980.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinesterase (EC:3.1.1.8)
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase
Gene namesi
Name:BCHE
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 88Sequence Analysis
Chaini9 – 581573CholinesterasePRO_0000008616Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi72 ↔ 99By similarity
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
Modified residuei205 – 2051PhosphoserineBy similarity
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi259 ↔ 270By similarity
Glycosylationi263 – 2631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi348 – 3481N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi407 ↔ 526By similarity
Glycosylationi462 – 4621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi488 – 4881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi493 – 4931N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi578 – 578InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Present in most cells except erythrocytes.

Interactioni

Subunit structurei

Homotetramer; disulfide-linked. Dimer of dimers (By similarity).By similarity

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000006971.

Structurei

3D structure databases

ProteinModelPortaliP21927.
SMRiP21927. Positions 11-541, 543-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2272.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiP21927.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTRSSHTED VIITTKNGRI RGINLPVFGG TVTAFLGIPY AQPPLGRLRF
60 70 80 90 100
KKPQSLTKWS DIWNATKYAN SCCQNIDQSF PGFHGSEMWN PNTDLSEDCL
110 120 130 140 150
YLNVWIPTPK PKNATVMIWI YGGGFQTGTS SLQVYDGKFL TRVERVIVVS
160 170 180 190 200
MNYRVGALGF LALPGNPEAP GNMGLFDQQL ALQWVQKNIA AFGGNPKSVT
210 220 230 240 250
LFGESAGAAS VSLHLLSPRS HPLFTRAILQ SGSSNAPWEV MSLHEARNRT
260 270 280 290 300
LTLAKFVGCS TENETEIIKC LRNKDAQEIL LNEVFVVPFD SLLSVNFGPT
310 320 330 340 350
VDGDFLTDMP DTLLQLGQLK KTQILVGVNK DEGTAFLVYG APGFSKDNTS
360 370 380 390 400
IITRKEFQEG LKIFFPGVSE FGKESILFHY TDWVDEQRPE NYREALDDVV
410 420 430 440 450
GDYNFICPAL EFTKKFSEWG NNAFFYYFEH RSSKLPWPEW MGVMHGYEIE
460 470 480 490 500
FVFGLPLERR VNYTKAEEIL SRSIMKRWAN FAKYGNPNGT QNNSTRWPVF
510 520 530 540 550
KSTEQKYLTL NTESPRIYTK LRAQQCRFWT LFFPKVLEMT GNIDEAEQEW
560 570 580
KAGFHRWNNY MMAWKNHFND YTSKKERCAG F
Length:581
Mass (Da):66,156
Last modified:May 1, 1991 - v1
Checksum:iFE8B199E7B32EB0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52090, X52091, X52092 Genomic DNA. Translation: CAA36308.1.
M62779 Genomic DNA. Translation: AAA31169.1.
PIRiS10255. C39768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52090, X52091, X52092 Genomic DNA. Translation: CAA36308.1.
M62779 Genomic DNA. Translation: AAA31169.1.
PIRiS10255. C39768.

3D structure databases

ProteinModelPortaliP21927.
SMRiP21927. Positions 11-541, 543-572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000006971.

Protein family/group databases

MEROPSiS09.980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiCOG2272.
HOGENOMiHOG000091866.
HOVERGENiHBG008839.
InParanoidiP21927.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of rabbit butyrylcholinesterase."
    Jbilo O., Roudani S., Chatonnet A.
    Nucleic Acids Res. 18:3990-3990(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: New Zealand.
  2. "Use of the polymerase chain reaction for homology probing of butyrylcholinesterase from several vertebrates."
    Arpagaus M., Chatonnet A., Masson P., Newton M., Vaughan T.A., Bartels C.F., Nogueira C.P., la Du B.N., Lockridge O.
    J. Biol. Chem. 266:6966-6974(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-215.
    Tissue: Liver.

Entry informationi

Entry nameiCHLE_RABIT
AccessioniPrimary (citable) accession number: P21927
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: March 4, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.