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P21927 (CHLE_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholinesterase

EC=3.1.1.8
Alternative name(s):
Acylcholine acylhydrolase
Butyrylcholine esterase
Choline esterase II
Pseudocholinesterase
Gene names
Name:BCHE
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length581 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters By similarity.

Catalytic activity

An acylcholine + H2O = choline + a carboxylate.

Subunit structure

Homotetramer; disulfide-linked. Dimer of dimers By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Present in most cells except erythrocytes.

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 88 Potential
Chain9 – 581573Cholinesterase
PRO_0000008616

Regions

Region123 – 1242Substrate binding By similarity

Sites

Active site2051Acyl-ester intermediate By similarity
Active site3321Charge relay system By similarity
Active site4451Charge relay system By similarity

Amino acid modifications

Modified residue2051Phosphoserine By similarity
Glycosylation641N-linked (GlcNAc...) Potential
Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation2481N-linked (GlcNAc...) Potential
Glycosylation2631N-linked (GlcNAc...) Potential
Glycosylation3481N-linked (GlcNAc...) Potential
Glycosylation4621N-linked (GlcNAc...) Potential
Glycosylation4881N-linked (GlcNAc...) Potential
Glycosylation4921N-linked (GlcNAc...) Potential
Glycosylation4931N-linked (GlcNAc...) Potential
Disulfide bond72 ↔ 99 By similarity
Disulfide bond259 ↔ 270 By similarity
Disulfide bond407 ↔ 526 By similarity
Disulfide bond578Interchain By similarity

Sequences

Sequence LengthMass (Da)Tools
P21927 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: FE8B199E7B32EB0A

FASTA58166,156
        10         20         30         40         50         60 
MVTRSSHTED VIITTKNGRI RGINLPVFGG TVTAFLGIPY AQPPLGRLRF KKPQSLTKWS 

        70         80         90        100        110        120 
DIWNATKYAN SCCQNIDQSF PGFHGSEMWN PNTDLSEDCL YLNVWIPTPK PKNATVMIWI 

       130        140        150        160        170        180 
YGGGFQTGTS SLQVYDGKFL TRVERVIVVS MNYRVGALGF LALPGNPEAP GNMGLFDQQL 

       190        200        210        220        230        240 
ALQWVQKNIA AFGGNPKSVT LFGESAGAAS VSLHLLSPRS HPLFTRAILQ SGSSNAPWEV 

       250        260        270        280        290        300 
MSLHEARNRT LTLAKFVGCS TENETEIIKC LRNKDAQEIL LNEVFVVPFD SLLSVNFGPT 

       310        320        330        340        350        360 
VDGDFLTDMP DTLLQLGQLK KTQILVGVNK DEGTAFLVYG APGFSKDNTS IITRKEFQEG 

       370        380        390        400        410        420 
LKIFFPGVSE FGKESILFHY TDWVDEQRPE NYREALDDVV GDYNFICPAL EFTKKFSEWG 

       430        440        450        460        470        480 
NNAFFYYFEH RSSKLPWPEW MGVMHGYEIE FVFGLPLERR VNYTKAEEIL SRSIMKRWAN 

       490        500        510        520        530        540 
FAKYGNPNGT QNNSTRWPVF KSTEQKYLTL NTESPRIYTK LRAQQCRFWT LFFPKVLEMT 

       550        560        570        580 
GNIDEAEQEW KAGFHRWNNY MMAWKNHFND YTSKKERCAG F 

« Hide

References

[1]"Complete sequence of rabbit butyrylcholinesterase."
Jbilo O., Roudani S., Chatonnet A.
Nucleic Acids Res. 18:3990-3990(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: New Zealand.
[2]"Use of the polymerase chain reaction for homology probing of butyrylcholinesterase from several vertebrates."
Arpagaus M., Chatonnet A., Masson P., Newton M., Vaughan T.A., Bartels C.F., Nogueira C.P., la Du B.N., Lockridge O.
J. Biol. Chem. 266:6966-6974(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-215.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X52090, X52091, X52092 Genomic DNA. Translation: CAA36308.1.
M62779 Genomic DNA. Translation: AAA31169.1.
PIRC39768. S10255.

3D structure databases

ProteinModelPortalP21927.
SMRP21927. Positions 11-541, 543-572.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000006971.

Protein family/group databases

MEROPSS09.980.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG2272.
HOGENOMHOG000091866.
HOVERGENHBG008839.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamPF08674. AChE_tetra. 1 hit.
PF00135. COesterase. 1 hit.
[Graphical view]
PRINTSPR00878. CHOLNESTRASE.
ProDomPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHLE_RABIT
AccessionPrimary (citable) accession number: P21927
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: June 11, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families