P21926 (CD9_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD9 antigen

Alternative name(s):
5H9 antigen
Cell growth-inhibiting gene 2 protein
Leukocyte antigen MIC3
Motility-related protein
Short name=MRP-1
Tetraspanin-29
Short name=Tspan-29
p24
CD_antigen=CD9

Gene names

Name:	CD9
Synonyms:	MIC3, TSPAN29
ORF Names:	GIG2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Ref.11 Ref.12 Ref.13
Subunit structure	Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. Ref.14 Ref.16 Ref.17
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed by a variety of hematopoietic and epithelial cells.
Post-translational modification	Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
Biological process	Cell adhesion Fertilization
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from electronic annotation. Source: Compara cell adhesion Inferred from direct assay Ref.12. Source: UniProtKB cellular component movement Inferred from direct assay Ref.11. Source: UniProtKB fusion of sperm to egg plasma membrane Inferred from direct assay Ref.13. Source: UniProtKB negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara oligodendrocyte development Inferred from electronic annotation. Source: Compara paranodal junction assembly Inferred from sequence or structural similarity. Source: UniProtKB platelet activation Non-traceable author statement Ref.2. Source: UniProtKB platelet degranulation Traceable author statement. Source: Reactome response to water deprivation Inferred from electronic annotation. Source: Compara
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara external side of plasma membrane Inferred from electronic annotation. Source: Compara integral to plasma membrane Non-traceable author statement Ref.2. Source: UniProtKB platelet alpha granule membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.10
	Chain	2 – 228	227	CD9 antigen		PRO_0000219205
Regions
	Topological domain	2 – 12	11	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Helical; Potential
	Topological domain	34 – 55	22	Extracellular Potential
	Transmembrane	56 – 76	21	Helical; Potential
	Topological domain	77 – 87	11	Cytoplasmic Potential
	Transmembrane	88 – 111	24	Helical; Potential
	Topological domain	112 – 195	84	Extracellular Potential
	Transmembrane	196 – 221	26	Helical; Potential
	Topological domain	222 – 228	7	Cytoplasmic Potential
Amino acid modifications
	Lipidation	9	1	S-palmitoyl cysteine Probable
	Lipidation	78	1	S-palmitoyl cysteine Probable
	Lipidation	79	1	S-palmitoyl cysteine Probable
	Lipidation	87	1	S-palmitoyl cysteine Probable
	Lipidation	218	1	S-palmitoyl cysteine Probable
	Lipidation	219	1	S-palmitoyl cysteine Probable
	Glycosylation	52	1	N-linked (GlcNAc...) Potential
	Glycosylation	53	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	9	1	C → A: Loss of palmitoylation; when associated with A-78; A-79; A-87; A-218 and A-219. Ref.15
	Mutagenesis	78	1	C → A: Loss of palmitoylation; when associated with A-9; A-79; A-87; A-218 and A-219. Ref.15
	Mutagenesis	79	1	C → A: Loss of palmitoylation; when associated with A-9; A-78; A-87; A-218 and A-219. Ref.15
	Mutagenesis	87	1	C → A: Loss of palmitoylation; when associated with A-9; A-78; A-79; A-218 and A-219. Ref.15
	Mutagenesis	218	1	C → A: Loss of palmitoylation; when associated with A-9; A-78; A-79; A-87 and A-219. Ref.15
	Mutagenesis	219	1	C → A: Loss of palmitoylation; when associated with A-9; A-78; A-79; A-87 and A-218. Ref.15
	Sequence conflict	215	1	M → T in AAH11988. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P21926 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: F68333E0C20611D8	FASTA	228	25,416
	10 20 30 40 50 60 MPVKGGTKCI KYLLFGFNFI FWLAGIAVLA IGLWLRFDSQ TKSIFEQETN NNNSSFYTGV 70 80 90 100 110 120 YILIGAGALM MLVGFLGCCG AVQESQCMLG LFFGFLLVIF AIEIAAAIWG YSHKDEVIKE 130 140 150 160 170 180 VQEFYKDTYN KLKTKDEPQR ETLKAIHYAL NCCGLAGGVE QFISDICPKK DVLETFTVKS 190 200 210 220 CPDAIKEVFD NKFHIIGAVG IGIAVVMIFG MIFSMILCCA IRRNREMV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the CD9 antigen. A new family of cell surface proteins." Boucheix C., Benoit P., Frachet P., Billard M., Worthington R.E., Gagnon J., Uzan G. J. Biol. Chem. 266:117-122(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-5.
[2]	"cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins." Lanza F., Wolf D., Fox C.F., Kieffer N., Seyer J.M., Fried V.A., Coughlin S.R., Phillips D.R., Jennings L.K. J. Biol. Chem. 266:10638-10645(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[3]	"Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31-15, which inhibits cell motility." Miyake M., Koyama M., Seno M., Ikeyama S. J. Exp. Med. 174:1347-1354(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Organization of the human CD9 gene." Rubinstein E., Benoit P., Billard M., Plaisance S., Prenant M., Uzan G., Boucheix C. Genomics 16:132-138(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leukocyte.
[5]	"The tetraspanin CD9 is preferentially expressed on the human CD4(+)CD45RA+ naive T cell population and is involved in T cell activation." Kobayashi H., Hosono O., Iwata S., Kawasaki H., Kuwana M., Tanaka H., Dang N.H., Morimoto C. Clin. Exp. Immunol. 137:101-108(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"Identification of a human growth inhibition gene 2 (GIG2)." Kim J.W. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	SeattleSNPs variation discovery resource Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[10]	"Purification and partial characterization of CD9 antigen of human platelets." Higashihara M., Takahata K., Yatomi Y., Nakahara K., Kurokawa K. FEBS Lett. 264:270-274(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21. Tissue: Platelet.
[11]	"Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA." Ikeyama S., Koyama M., Yamaoko M., Sasada R., Miyake M. J. Exp. Med. 177:1231-1237(1993) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN CELL MOTILITY AND METASTASIS.
[12]	"CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin." Masellis-Smith A., Shaw A.R. J. Immunol. 152:2768-2777(1994) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN CELL ADHESION.
[13]	"Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion." Higginbottom A., Takahashi Y., Bolling L., Coonrod S.A., White J.M., Partridge L.J., Monk P.N. Biochem. Biophys. Res. Commun. 311:208-214(2003) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN SPERM-EGG FUSION.
[14]	"Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking." Kovalenko O.V., Yang X., Kolesnikova T.V., Hemler M.E. Biochem. J. 377:407-417(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
[15]	"Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation." Charrin S., Manie S., Oualid M., Billard M., Boucheix C., Rubinstein E. FEBS Lett. 516:139-144(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PALMITOYLATION AT CYS-9; CYS-78; CYS-79; CYS-87; CYS-218 AND CYS-219, MUTAGENESIS OF CYS-9; CYS-78; CYS-79; CYS-87; CYS-218 AND CYS-219.
[16]	"The major CD9 and CD81 molecular partner. Identification and characterization of the complexes." Charrin S., Le Naour F., Oualid M., Billard M., Faure G., Hanash S.M., Boucheix C., Rubinstein E. J. Biol. Chem. 276:14329-14337(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PTGFRN.
[17]	"EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily." Stipp C.S., Kolesnikova T.V., Hemler M.E. J. Biol. Chem. 276:40545-40554(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IGSF8.
[18]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Web resources

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38690 mRNA. Translation: AAA80320.1. L34068 mRNA. Translation: AAA59982.1. X60111 mRNA. Translation: CAA42708.1. S60489 , S60462, S60463, S60464, S60700, S60699, S60465, S60472 Genomic DNA. Translation: AAC60586.1. L08118 Genomic DNA. No translation available. L08119 Genomic DNA. Translation: AAA51954.1. Sequence problems. L08120 Genomic DNA. Translation: AAA51955.1. Sequence problems. L08121 Genomic DNA. Translation: AAA51956.1. L08122 Genomic DNA. Translation: AAA51957.1. L08123 Genomic DNA. Translation: AAA51958.1. L08124 Genomic DNA. Translation: AAA51959.1. L08125 Genomic DNA. No translation available. AB079244 mRNA. Translation: BAE71132.1. AY423720 mRNA. Translation: AAS00483.1. AY422198 Genomic DNA. Translation: AAQ87878.1. CH471116 Genomic DNA. Translation: EAW88812.1. CH471116 Genomic DNA. Translation: EAW88813.1. BC011988 mRNA. Translation: AAH11988.1.
IPI	IPI00215997.
PIR	A40402. A46123.
RefSeq	NP_001760.1. NM_001769.3.
UniGene	Hs.114286. Hs.712104.
3D structure databases
ProteinModelPortal	P21926.
SMR	P21926. Positions 111-191.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1122N.
IntAct	P21926. 1 interaction.
STRING	9606.ENSP00000009180.
PTM databases
PhosphoSite	P21926.
Polymorphism databases
DMDM	231724.
Proteomic databases
PaxDb	P21926.
PRIDE	P21926.
Protocols and materials databases
DNASU	928.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000009180; ENSP00000009180; ENSG00000010278. ENST00000382518; ENSP00000371958; ENSG00000010278.
GeneID	928.
KEGG	hsa:928.
UCSC	uc001qnq.2. human.
Organism-specific databases
CTD	928.
GeneCards	GC12P006308.
HGNC	HGNC:1709. CD9.
HPA	CAB002490.
MIM	143030. gene.
neXtProt	NX_P21926.
PharmGKB	PA26247.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG242618.
HOGENOM	HOG000230651.
HOVERGEN	HBG002324.
InParanoid	P21926.
KO	K06460.
OMA	VKPCPEA.
OrthoDB	EOG402WT9.
PhylomeDB	P21926.
Enzyme and pathway databases
Pathway_Interaction_DB	a6b1_a6b4_integrin_pathway. a6b1 and a6b4 Integrin signaling.
Reactome	REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P21926.
Bgee	P21926.
CleanEx	HS_CD9.
Genevestigator	P21926.
GermOnline	ENSG00000010278. Homo sapiens.
Family and domain databases
InterPro	IPR000301. Tetraspanin. IPR018499. Tetraspanin/Peripherin. IPR018503. Tetraspanin_CS. IPR008952. Tetraspanin_EC2. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PIRSF	PIRSF002419. Tetraspanin. 1 hit.
PRINTS	PR00259. TMFOUR.
SUPFAM	SSF48652. Tetraspanin. 1 hit.
PROSITE	PS00421. TM4_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CD9. human.
GenomeRNAi	928.
NextBio	3846.
SOURCE	Search...

Entry information

Entry name

CD9_HUMAN

Accession

Primary (citable) accession number: P21926
Secondary accession number(s): D3DUQ9, Q5J7W6, Q96ES4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 136 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.