CD9 antigen - Homo sapiens (Human)

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Reviewed, UniProtKB/Swiss-Prot P21926 (CD9_HUMAN)

Last modified May 5, 2009. Version 99. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    CD9 antigen
Alternative name(s):
    p24
    Leukocyte antigen MIC3
    Motility-related protein
      Short name=MRP-1
    Tetraspanin-29
      Short name=Tspan-29
    5H9 antigen
    Cell growth-inhibiting gene 2 protein
    CD_antigen=CD9

Gene names

Name:	CD9
Synonyms:	MIC3, TSPAN29
ORF Names:	GIG2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Ref.11 Ref.12 Ref.13
Subunit structure	Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. Ref.14 Ref.16 Ref.17
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed by a variety of hematopoietic and epithelial cells.
Post-translational modification	Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

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Ontologies

Keywords
Biological process	Cell adhesion Fertilization
Cellular component	Membrane
Domain	Transmembrane
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell adhesion Ref.12 Inferred from direct assay. Source: UniProtKB cell motion Ref.11 Inferred from direct assay. Source: UniProtKB fusion of sperm to egg plasma membrane Ref.13 Inferred from direct assay. Source: UniProtKB paranodal junction assembly Inferred from sequence or structural similarity. Source: UniProtKB platelet activation Ref.2 Non-traceable author statement. Source: UniProtKB
Cellular component	integral to plasma membrane Ref.1 Ref.2 Non-traceable author statement. Source: UniProtKB platelet alpha granule membrane Inferred from Experiment. Source: Reactome
Molecular function	protein binding Ref.16 Ref.17 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.1 Ref.10

Chain

2 – 228

227

CD9 antigen

PRO_0000219205

Regions

Topological domain

2 – 12

Cytoplasmic Potential

Transmembrane

13 – 33

Potential

Topological domain

34 – 55

Extracellular Potential

Transmembrane

56 – 76

Potential

Topological domain

77 – 87

Cytoplasmic Potential

Transmembrane

88 – 111

Potential

Topological domain

112 – 195

Extracellular Potential

Transmembrane

196 – 221

Potential

Topological domain

222 – 228

Cytoplasmic Potential

Amino acid modifications

Lipidation

S-palmitoyl cysteine Probable

Lipidation

S-palmitoyl cysteine Probable

Lipidation

S-palmitoyl cysteine Probable

Lipidation

S-palmitoyl cysteine Probable

Lipidation

218

S-palmitoyl cysteine Probable

Lipidation

219

S-palmitoyl cysteine Probable

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis

C → A: Loss of palmitoylation; when associated with A-78; A-79; A-87; A-218 and A-219. Ref.15

Mutagenesis

C → A: Loss of palmitoylation; when associated with A-9; A-79; A-87; A-218 and A-219. Ref.15

Mutagenesis

C → A: Loss of palmitoylation; when associated with A-9; A-78; A-87; A-218 and A-219. Ref.15

Mutagenesis

C → A: Loss of palmitoylation; when associated with A-9; A-78; A-79; A-218 and A-219. Ref.15

Mutagenesis

218

C → A: Loss of palmitoylation; when associated with A-9; A-78; A-79; A-87 and A-219. Ref.15

Mutagenesis

219

C → A: Loss of palmitoylation; when associated with A-9; A-78; A-79; A-87 and A-218. Ref.15

Sequence conflict

215

M → T in AAH11988. Ref.9

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Sequences

Sequence

Length

Mass (Da)

Tools

P21926-1 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: F68333E0C20611D8
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FASTA

228

25,416

        10         20         30         40         50         60 
MPVKGGTKCI KYLLFGFNFI FWLAGIAVLA IGLWLRFDSQ TKSIFEQETN NNNSSFYTGV 

        70         80         90        100        110        120 
YILIGAGALM MLVGFLGCCG AVQESQCMLG LFFGFLLVIF AIEIAAAIWG YSHKDEVIKE 

       130        140        150        160        170        180 
VQEFYKDTYN KLKTKDEPQR ETLKAIHYAL NCCGLAGGVE QFISDICPKK DVLETFTVKS 

       190        200        210        220 
CPDAIKEVFD NKFHIIGAVG IGIAVVMIFG MIFSMILCCA IRRNREMV

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the CD9 antigen. A new family of cell surface proteins." Boucheix C., Benoit P., Frachet P., Billard M., Worthington R.E., Gagnon J., Uzan G. J. Biol. Chem. 266:117-122(1991) [PubMed: 1840589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-5.
[2]	"cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins." Lanza F., Wolf D., Fox C.F., Kieffer N., Seyer J.M., Fried V.A., Coughlin S.R., Phillips D.R., Jennings L.K. J. Biol. Chem. 266:10638-10645(1991) [PubMed: 2037603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[3]	"Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31-15, which inhibits cell motility." Miyake M., Koyama M., Seno M., Ikeyama S. J. Exp. Med. 174:1347-1354(1991) [PubMed: 1720807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Organization of the human CD9 gene." Rubinstein E., Benoit P., Billard M., Plaisance S., Prenant M., Uzan G., Boucheix C. Genomics 16:132-138(1993) [PubMed: 8486348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leukocyte.
[5]	"The tetraspanin CD9 is preferentially expressed on the human CD4(+)CD45RA+ naive T cell population and is involved in T cell activation." Kobayashi H., Hosono O., Iwata S., Kawasaki H., Kuwana M., Tanaka H., Dang N.H., Morimoto C. Clin. Exp. Immunol. 137:101-108(2004) [PubMed: 15196249] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"Identification of a human growth inhibition gene 2 (GIG2)." Kim J.W. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	SeattleSNPs variation discovery resource Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[10]	"Purification and partial characterization of CD9 antigen of human platelets." Higashihara M., Takahata K., Yatomi Y., Nakahara K., Kurokawa K. FEBS Lett. 264:270-274(1990) [PubMed: 2358073] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21. Tissue: Platelet.
[11]	"Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA." Ikeyama S., Koyama M., Yamaoko M., Sasada R., Miyake M. J. Exp. Med. 177:1231-1237(1993) [PubMed: 8478605] [Abstract] Cited for: ROLE IN CELL MOTILITY AND METASTASIS.
[12]	"CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin." Masellis-Smith A., Shaw A.R. J. Immunol. 152:2768-2777(1994) [PubMed: 7511626] [Abstract] Cited for: ROLE IN CELL ADHESION.
[13]	"Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion." Higginbottom A., Takahashi Y., Bolling L., Coonrod S.A., White J.M., Partridge L.J., Monk P.N. Biochem. Biophys. Res. Commun. 311:208-214(2003) [PubMed: 14575715] [Abstract] Cited for: ROLE IN SPERM-EGG FUSION.
[14]	"Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking." Kovalenko O.V., Yang X., Kolesnikova T.V., Hemler M.E. Biochem. J. 377:407-417(2004) [PubMed: 14556650] [Abstract] Cited for: SUBUNIT.
[15]	"Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation." Charrin S., Manie S., Oualid M., Billard M., Boucheix C., Rubinstein E. FEBS Lett. 516:139-144(2002) [PubMed: 11959120] [Abstract] Cited for: PALMITOYLATION AT CYS-9; CYS-78; CYS-79; CYS-87; CYS-218 AND CYS-219, MUTAGENESIS OF CYS-9; CYS-78; CYS-79; CYS-87; CYS-218 AND CYS-219.
[16]	"The major CD9 and CD81 molecular partner. Identification and characterization of the complexes." Charrin S., Le Naour F., Oualid M., Billard M., Faure G., Hanash S.M., Boucheix C., Rubinstein E. J. Biol. Chem. 276:14329-14337(2001) [PubMed: 11278880] [Abstract] Cited for: INTERACTION WITH PTGFRN.
[17]	"EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily." Stipp C.S., Kolesnikova T.V., Hemler M.E. J. Biol. Chem. 276:40545-40554(2001) [PubMed: 11504738] [Abstract] Cited for: INTERACTION WITH IGSF8.
[18]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Web resources

SeattleSNPs

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Cross-references

Sequence databases
	M38690 mRNA. Translation: AAA80320.1. L34068 mRNA. Translation: AAA59982.1. X60111 mRNA. Translation: CAA42708.1. S60489 S60472 Genomic DNA. Translation: AAC60586.1. L08118 Genomic DNA. No translation available. L08119 Genomic DNA. Translation: AAA51954.1. Sequence problems. L08120 Genomic DNA. Translation: AAA51955.1. Sequence problems. L08121 Genomic DNA. Translation: AAA51956.1. L08122 Genomic DNA. Translation: AAA51957.1. L08123 Genomic DNA. Translation: AAA51958.1. L08124 Genomic DNA. Translation: AAA51959.1. L08125 Genomic DNA. No translation available. AB079244 mRNA. Translation: BAE71132.1. AY423720 mRNA. Translation: AAS00483.1. AY422198 Genomic DNA. Translation: AAQ87878.1. CH471116 Genomic DNA. Translation: EAW88812.1. BC011988 mRNA. Translation: AAH11988.1.
IPI	IPI00215997.
PIR	A40402. A46123.
RefSeq	NP_001760.1.
UniGene	Hs.114286
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1122N.
Proteomic databases
PRIDE	P21926.
Genome annotation databases
Ensembl	ENSG00000010278. Homo sapiens. [Contig view]
GeneID	928.
KEGG	hsa:928.
Organism-specific databases
GeneCards	GC12P006179.
H-InvDB	HIX0010357.
HGNC	HGNC:1709. CD9.
HPA	CAB002490.
MIM	143030. gene.
PharmGKB	PA26247.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P21926.
HOVERGEN	P21926.
OMA	P21926. DICPKKD.
Enzyme and pathway databases
Pathway_Interaction_DB	a6b1_a6b4_integrin_pathway. a6b1 and a6b4 Integrin signaling.
Reactome	REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P21926.
Bgee	P21926.
CleanEx	HS_CD9.
GermOnline	ENSG00000010278. Homo sapiens.
Family and domain databases
InterPro	IPR018499. Tetraspanin. IPR018503. Tetraspanin_CS. IPR000301. Tetraspanin_sub. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PRINTS	PR00259. TMFOUR.
PROSITE	PS00421. TM4_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	3846.
SOURCE	Search...

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Entry information

Entry name

CD9_HUMAN

Accession

Primary (citable) accession number: P21926
Secondary accession number(s): Q5J7W6, Q96ES4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	January 23, 2007
Last modified:	May 5, 2009
This is version 99 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents