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P21926

- CD9_HUMAN

UniProt

P21926 - CD9_HUMAN

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Protein

CD9 antigen

Gene

CD9

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.3 Publications

GO - Biological processi

  1. blood coagulation Source: Reactome
  2. brain development Source: Ensembl
  3. cell adhesion Source: UniProtKB
  4. cellular component movement Source: UniProtKB
  5. fusion of sperm to egg plasma membrane Source: UniProtKB
  6. multicellular organism reproduction Source: Reactome
  7. negative regulation of cell proliferation Source: Ensembl
  8. oligodendrocyte development Source: Ensembl
  9. paranodal junction assembly Source: UniProtKB
  10. platelet activation Source: UniProtKB
  11. platelet degranulation Source: Reactome
  12. response to water deprivation Source: Ensembl
  13. single fertilization Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Fertilization

Enzyme and pathway databases

ReactomeiREACT_164006. Sperm:Oocyte Membrane Binding.

Protein family/group databases

TCDBi8.A.40.1.9. the tetraspanin (tetraspanin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
CD9 antigen
Alternative name(s):
5H9 antigen
Cell growth-inhibiting gene 2 protein
Leukocyte antigen MIC3
Motility-related protein
Short name:
MRP-1
Tetraspanin-29
Short name:
Tspan-29
p24
CD_antigen: CD9
Gene namesi
Name:CD9
Synonyms:MIC3, TSPAN29
ORF Names:GIG2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:1709. CD9.

Subcellular locationi

Membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. apical plasma membrane Source: Ensembl
  2. external side of plasma membrane Source: Ensembl
  3. extracellular space Source: UniProt
  4. extracellular vesicular exosome Source: UniProtKB
  5. focal adhesion Source: UniProtKB
  6. integral component of plasma membrane Source: UniProtKB
  7. plasma membrane Source: Reactome
  8. platelet alpha granule membrane Source: Reactome
  9. vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi9 – 91C → A: Loss of palmitoylation; when associated with A-78; A-79; A-87; A-218 and A-219. 1 Publication
Mutagenesisi78 – 781C → A: Loss of palmitoylation; when associated with A-9; A-79; A-87; A-218 and A-219. 1 Publication
Mutagenesisi79 – 791C → A: Loss of palmitoylation; when associated with A-9; A-78; A-87; A-218 and A-219. 1 Publication
Mutagenesisi87 – 871C → A: Loss of palmitoylation; when associated with A-9; A-78; A-79; A-218 and A-219. 1 Publication
Mutagenesisi218 – 2181C → A: Loss of palmitoylation; when associated with A-9; A-78; A-79; A-87 and A-219. 1 Publication
Mutagenesisi219 – 2191C → A: Loss of palmitoylation; when associated with A-9; A-78; A-79; A-87 and A-218. 1 Publication

Organism-specific databases

PharmGKBiPA26247.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 228227CD9 antigenPRO_0000219205Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi9 – 91S-palmitoyl cysteine1 Publication
Glycosylationi52 – 521N-linked (GlcNAc...)Sequence Analysis
Glycosylationi53 – 531N-linked (GlcNAc...)Sequence Analysis
Lipidationi78 – 781S-palmitoyl cysteine1 Publication
Lipidationi79 – 791S-palmitoyl cysteine1 Publication
Lipidationi87 – 871S-palmitoyl cysteine1 Publication
Lipidationi218 – 2181S-palmitoyl cysteine1 Publication
Lipidationi219 – 2191S-palmitoyl cysteine1 Publication

Post-translational modificationi

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro). The protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

MaxQBiP21926.
PaxDbiP21926.
PRIDEiP21926.

PTM databases

PhosphoSiteiP21926.

Expressioni

Tissue specificityi

Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells.1 Publication

Gene expression databases

BgeeiP21926.
CleanExiHS_CD9.
ExpressionAtlasiP21926. baseline and differential.
GenevestigatoriP21926.

Organism-specific databases

HPAiCAB002490.

Interactioni

Subunit structurei

Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Interacts with CD63. Identified in a complex with CD63 and ITGB3. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MMEP084736EBI-4280101,EBI-353759

Protein-protein interaction databases

BioGridi107366. 23 interactions.
DIPiDIP-1122N.
IntActiP21926. 24 interactions.
MINTiMINT-1527350.
STRINGi9606.ENSP00000009180.

Structurei

3D structure databases

ProteinModelPortaliP21926.
SMRiP21926. Positions 110-191.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1211CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini34 – 5522ExtracellularSequence AnalysisAdd
BLAST
Topological domaini77 – 8711CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini112 – 19584ExtracellularSequence AnalysisAdd
BLAST
Topological domaini222 – 2287CytoplasmicSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence AnalysisAdd
BLAST
Transmembranei56 – 7621HelicalSequence AnalysisAdd
BLAST
Transmembranei88 – 11124HelicalSequence AnalysisAdd
BLAST
Transmembranei196 – 22126HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG242618.
GeneTreeiENSGT00760000118983.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
InParanoidiP21926.
KOiK06460.
OMAiALNCCGI.
OrthoDBiEOG7FBRJV.
PhylomeDBiP21926.
TreeFamiTF352895.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiIPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21926-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPVKGGTKCI KYLLFGFNFI FWLAGIAVLA IGLWLRFDSQ TKSIFEQETN
60 70 80 90 100
NNNSSFYTGV YILIGAGALM MLVGFLGCCG AVQESQCMLG LFFGFLLVIF
110 120 130 140 150
AIEIAAAIWG YSHKDEVIKE VQEFYKDTYN KLKTKDEPQR ETLKAIHYAL
160 170 180 190 200
NCCGLAGGVE QFISDICPKK DVLETFTVKS CPDAIKEVFD NKFHIIGAVG
210 220
IGIAVVMIFG MIFSMILCCA IRRNREMV
Length:228
Mass (Da):25,416
Last modified:January 23, 2007 - v4
Checksum:iF68333E0C20611D8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti215 – 2151M → T in AAH11988. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M38690 mRNA. Translation: AAA80320.1.
L34068 mRNA. Translation: AAA59982.1.
X60111 mRNA. Translation: CAA42708.1.
S60489
, S60462, S60463, S60464, S60700, S60699, S60465, S60472 Genomic DNA. Translation: AAC60586.1.
L08118 Genomic DNA. No translation available.
L08119 Genomic DNA. Translation: AAA51954.1. Sequence problems.
L08120 Genomic DNA. Translation: AAA51955.1. Sequence problems.
L08121 Genomic DNA. Translation: AAA51956.1.
L08122 Genomic DNA. Translation: AAA51957.1.
L08123 Genomic DNA. Translation: AAA51958.1.
L08124 Genomic DNA. Translation: AAA51959.1.
L08125 Genomic DNA. No translation available.
AB079244 mRNA. Translation: BAE71132.1.
AY423720 mRNA. Translation: AAS00483.1.
AY422198 Genomic DNA. Translation: AAQ87878.1.
CH471116 Genomic DNA. Translation: EAW88812.1.
CH471116 Genomic DNA. Translation: EAW88813.1.
BC011988 mRNA. Translation: AAH11988.1.
CCDSiCCDS8540.1.
PIRiA46123. A40402.
RefSeqiNP_001760.1. NM_001769.3.
UniGeneiHs.114286.
Hs.712104.

Genome annotation databases

EnsembliENST00000009180; ENSP00000009180; ENSG00000010278.
ENST00000382518; ENSP00000371958; ENSG00000010278.
GeneIDi928.
KEGGihsa:928.
UCSCiuc001qnq.2. human.

Polymorphism databases

DMDMi231724.

Cross-referencesi

Web resourcesi

SeattleSNPs
Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M38690 mRNA. Translation: AAA80320.1 .
L34068 mRNA. Translation: AAA59982.1 .
X60111 mRNA. Translation: CAA42708.1 .
S60489
, S60462 , S60463 , S60464 , S60700 , S60699 , S60465 , S60472 Genomic DNA. Translation: AAC60586.1 .
L08118 Genomic DNA. No translation available.
L08119 Genomic DNA. Translation: AAA51954.1 . Sequence problems.
L08120 Genomic DNA. Translation: AAA51955.1 . Sequence problems.
L08121 Genomic DNA. Translation: AAA51956.1 .
L08122 Genomic DNA. Translation: AAA51957.1 .
L08123 Genomic DNA. Translation: AAA51958.1 .
L08124 Genomic DNA. Translation: AAA51959.1 .
L08125 Genomic DNA. No translation available.
AB079244 mRNA. Translation: BAE71132.1 .
AY423720 mRNA. Translation: AAS00483.1 .
AY422198 Genomic DNA. Translation: AAQ87878.1 .
CH471116 Genomic DNA. Translation: EAW88812.1 .
CH471116 Genomic DNA. Translation: EAW88813.1 .
BC011988 mRNA. Translation: AAH11988.1 .
CCDSi CCDS8540.1.
PIRi A46123. A40402.
RefSeqi NP_001760.1. NM_001769.3.
UniGenei Hs.114286.
Hs.712104.

3D structure databases

ProteinModelPortali P21926.
SMRi P21926. Positions 110-191.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107366. 23 interactions.
DIPi DIP-1122N.
IntActi P21926. 24 interactions.
MINTi MINT-1527350.
STRINGi 9606.ENSP00000009180.

Protein family/group databases

TCDBi 8.A.40.1.9. the tetraspanin (tetraspanin) family.

PTM databases

PhosphoSitei P21926.

Polymorphism databases

DMDMi 231724.

Proteomic databases

MaxQBi P21926.
PaxDbi P21926.
PRIDEi P21926.

Protocols and materials databases

DNASUi 928.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000009180 ; ENSP00000009180 ; ENSG00000010278 .
ENST00000382518 ; ENSP00000371958 ; ENSG00000010278 .
GeneIDi 928.
KEGGi hsa:928.
UCSCi uc001qnq.2. human.

Organism-specific databases

CTDi 928.
GeneCardsi GC12P006308.
HGNCi HGNC:1709. CD9.
HPAi CAB002490.
MIMi 143030. gene.
neXtProti NX_P21926.
PharmGKBi PA26247.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG242618.
GeneTreei ENSGT00760000118983.
HOGENOMi HOG000230651.
HOVERGENi HBG002324.
InParanoidi P21926.
KOi K06460.
OMAi ALNCCGI.
OrthoDBi EOG7FBRJV.
PhylomeDBi P21926.
TreeFami TF352895.

Enzyme and pathway databases

Reactomei REACT_164006. Sperm:Oocyte Membrane Binding.

Miscellaneous databases

ChiTaRSi CD9. human.
GeneWikii CD9.
GenomeRNAii 928.
NextBioi 3846.
PROi P21926.
SOURCEi Search...

Gene expression databases

Bgeei P21926.
CleanExi HS_CD9.
ExpressionAtlasi P21926. baseline and differential.
Genevestigatori P21926.

Family and domain databases

Gene3Di 1.10.1450.10. 1 hit.
InterProi IPR028977. CD81_LEL.
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view ]
Pfami PF00335. Tetraspannin. 1 hit.
[Graphical view ]
PIRSFi PIRSF002419. Tetraspanin. 1 hit.
PRINTSi PR00259. TMFOUR.
SUPFAMi SSF48652. SSF48652. 1 hit.
PROSITEi PS00421. TM4_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the CD9 antigen. A new family of cell surface proteins."
    Boucheix C., Benoit P., Frachet P., Billard M., Worthington R.E., Gagnon J., Uzan G.
    J. Biol. Chem. 266:117-122(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-5.
  2. "cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins."
    Lanza F., Wolf D., Fox C.F., Kieffer N., Seyer J.M., Fried V.A., Coughlin S.R., Phillips D.R., Jennings L.K.
    J. Biol. Chem. 266:10638-10645(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  3. "Identification of the motility-related protein (MRP-1), recognized by monoclonal antibody M31-15, which inhibits cell motility."
    Miyake M., Koyama M., Seno M., Ikeyama S.
    J. Exp. Med. 174:1347-1354(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Leukocyte.
  5. "The tetraspanin CD9 is preferentially expressed on the human CD4(+)CD45RA+ naive T cell population and is involved in T cell activation."
    Kobayashi H., Hosono O., Iwata S., Kawasaki H., Kuwana M., Tanaka H., Dang N.H., Morimoto C.
    Clin. Exp. Immunol. 137:101-108(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  6. "Identification of a human growth inhibition gene 2 (GIG2)."
    Kim J.W.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. SeattleSNPs variation discovery resource
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  10. "Purification and partial characterization of CD9 antigen of human platelets."
    Higashihara M., Takahata K., Yatomi Y., Nakahara K., Kurokawa K.
    FEBS Lett. 264:270-274(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21.
    Tissue: Platelet.
  11. "Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA."
    Ikeyama S., Koyama M., Yamaoko M., Sasada R., Miyake M.
    J. Exp. Med. 177:1231-1237(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN CELL MOTILITY AND METASTASIS.
  12. "CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin."
    Masellis-Smith A., Shaw A.R.
    J. Immunol. 152:2768-2777(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN CELL ADHESION.
  13. "Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion."
    Higginbottom A., Takahashi Y., Bolling L., Coonrod S.A., White J.M., Partridge L.J., Monk P.N.
    Biochem. Biophys. Res. Commun. 311:208-214(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN SPERM-EGG FUSION.
  14. "Evidence for specific tetraspanin homodimers: inhibition of palmitoylation makes cysteine residues available for cross-linking."
    Kovalenko O.V., Yang X., Kolesnikova T.V., Hemler M.E.
    Biochem. J. 377:407-417(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  15. "Differential stability of tetraspanin/tetraspanin interactions: role of palmitoylation."
    Charrin S., Manie S., Oualid M., Billard M., Boucheix C., Rubinstein E.
    FEBS Lett. 516:139-144(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-9; CYS-78; CYS-79; CYS-87; CYS-218 AND CYS-219, MUTAGENESIS OF CYS-9; CYS-78; CYS-79; CYS-87; CYS-218 AND CYS-219.
  16. "The major CD9 and CD81 molecular partner. Identification and characterization of the complexes."
    Charrin S., Le Naour F., Oualid M., Billard M., Faure G., Hanash S.M., Boucheix C., Rubinstein E.
    J. Biol. Chem. 276:14329-14337(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTGFRN.
  17. "EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily."
    Stipp C.S., Kolesnikova T.V., Hemler M.E.
    J. Biol. Chem. 276:40545-40554(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IGSF8.
  18. "Palmitoylation supports the association of tetraspanin CD63 with CD9 and integrin alphaIIbbeta3 in activated platelets."
    Israels S.J., McMillan-Ward E.M.
    Thromb. Res. 125:152-158(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMYTOYLATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH CD63, IDENTIFICATION IN A COMPLEX WITH ITGB3 AND CD63.
  19. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCD9_HUMAN
AccessioniPrimary (citable) accession number: P21926
Secondary accession number(s): D3DUQ9, Q5J7W6, Q96ES4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 152 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3