Reviewed, UniProtKB/Swiss-Prot P21920 (COBK_PSEDE)

Names and origin

Protein names

Recommended name:
    Precorrin-6A reductase
    EC=1.3.1.54
Alternative name(s):
    Precorrin-6X reductase

Gene names

Name:

cobK

Organism

Pseudomonas denitrificans

Taxonomic identifier

43306 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reduction of the macrocycle of precorrin-6X into precorrin-6Y.
Catalytic activity	Precorrin-6B + NADP(+) = precorrin-6A + NADPH.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinic acid a,c-diamide from precorrin-2 (aerobic route): step 6/10.
Sequence similarities	Belongs to the precorrin-6x reductase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 261	260	Precorrin-6A reductase		PRO_0000135920

Sequences

	Sequence		Length	Mass (Da)
	P21920-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: B0AE2E902D35F02C	FASTA	261	28,096
	10 20 30 40 50 60 MAGSLFDTSA MEKPRILILG GTTEARELAR RLAEDVRYDT AISLAGRTAD PRPQPVKTRI 70 80 90 100 110 120 GGFGGADGLA HFVHDENIAL LVDATHPFAA RISHNAADAA QRTGVALIAL RRPEWVPLPG 130 140 150 160 170 180 DRWTAVDSVV EAVSALGDRR RRVFLAIGRQ EAFHFEVAPQ HSYVIRSVDP VTPPLNLPDQ 190 200 210 220 230 240 EAILATGPFA EADEAALLRS RQIDVIVAKN SGGSATYGKI AAARRLGIEV IMVERRKPAD 250 260 VPTVGSCDEA LNRIAHWLAP A

References

[1]	"Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans fragment carrying eight genes involved in transformation of precorrin-2 to cobyrinic acid." Crouzet J., Cameron B., Cauchois L., Rigault S., Rouyez M.-C., Blanche F., Thibaut D., Debussche L. J. Bacteriol. 172:5980-5990(1990) [PubMed: 2211521] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SC510.
[2]	"Precorrin-6x reductase from Pseudomonas denitrificans: purification and characterization of the enzyme and identification of the structural gene." Blanche F., Thibaut D., Famechon A., Debussche L., Cameron B., Crouzet J. J. Bacteriol. 174:1036-1042(1992) [PubMed: 1732193] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-7; 60-69; 112-122 AND 143-148, SEQUENCE REVISION, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M59301 Genomic DNA. Translation: AAA25799.1. Sequence problems.
PIR	F36145.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-113.
Family and domain databases
InterPro	IPR003723. Cbl_synth_CobK/CbiJ. [Graphical view]
Pfam	PF02571. CbiJ. 1 hit. [Graphical view]
TIGRFAMs	TIGR00715. precor6x_red. 1 hit.
PROSITE	PS51014. COBK_CBIJ. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

COBK_PSEDE

Accession

Primary (citable) accession number: P21920

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	January 23, 2007
Last modified:	November 4, 2008
This is version 45 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)