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Protein

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Gene

Sdhb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).By similarity

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:

Pathway:itricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (Sdhb), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (Sdha)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi95 – 951Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi100 – 1001Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi103 – 1031Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi115 – 1151Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi188 – 1881Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi191 – 1911Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi194 – 1941Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi198 – 1981Iron-sulfur 3 (3Fe-4S)By similarity
Binding sitei203 – 2031Ubiquinone; shared with DHSDBy similarity
Metal bindingi245 – 2451Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi251 – 2511Iron-sulfur 3 (3Fe-4S)By similarity
Metal bindingi255 – 2551Iron-sulfur 2 (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

  • respiratory electron transport chain Source: RGD
  • succinate metabolic process Source: RGD
  • tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_338796. Citric acid cycle (TCA cycle).
REACT_347303. Respiratory electron transport.
SABIO-RKP21913.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Iron-sulfur subunit of complex II
Short name:
Ip
Gene namesi
Name:Sdhb
Synonyms:Sdh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1308598. Sdhb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030MitochondrionBy similarityAdd
BLAST
Chaini31 – 282252Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrialPRO_0000158693Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531N6-acetyllysineBy similarity
Modified residuei57 – 571N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP21913.
PRIDEiP21913.

PTM databases

PhosphoSiteiP21913.

Expressioni

Gene expression databases

GenevisibleiP21913. RN.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD.By similarity

Protein-protein interaction databases

IntActiP21913. 1 interaction.
STRINGi10116.ENSRNOP00000010593.

Structurei

3D structure databases

ProteinModelPortaliP21913.
SMRiP21913. Positions 39-277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 135802Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini178 – 208314Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0479.
GeneTreeiENSGT00390000013558.
HOGENOMiHOG000160590.
HOVERGENiHBG005483.
InParanoidiP21913.
KOiK00235.
OMAiIDSHERM.
OrthoDBiEOG7H4DTN.
PhylomeDBiP21913.
TreeFamiTF300754.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21913-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVVGVSLK RGFSATALGR VGLQFQACRE AQTAAAAAPR IKTFAIYRWD
60 70 80 90 100
PDKAGDKPRM QTYKVDLNKC GPMVLDALIK IKNEIDSTLT FRRSCREGIC
110 120 130 140 150
GSCAMNINGG NTLACTRRID TDLGKVSKIY PLPHMYVIKD LVPDLSNFYA
160 170 180 190 200
QYKSIEPYLK KKDESQEGKQ QYLQSIEDRE KLDGLYECIL CACCSTSCPS
210 220 230 240 250
YWWNGDKYLG PAVLMQAYRW MIDSRDEFTE ERLAKLQDPF SLYRCHTIMN
260 270 280
CTQTCPKGLN PGKAIAEIKK MMATYKEKRA LA
Length:282
Mass (Da):31,830
Last modified:September 2, 2008 - v2
Checksum:i6600EBC8201529A9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti122 – 1243DLG → NLN no nucleotide entry (PubMed:2494655).Curated
Sequence conflicti153 – 1531K → R no nucleotide entry (PubMed:2494655).Curated
Sequence conflicti178 – 1781D → E no nucleotide entry (PubMed:2494655).Curated
Sequence conflicti227 – 2271E → D no nucleotide entry (PubMed:2494655).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473968 Genomic DNA. Translation: EDL80954.1.
BC158620 mRNA. Translation: AAI58621.1.
DQ403001 mRNA. Translation: ABD77134.1.
PIRiB32394.
RefSeqiNP_001094009.1. NM_001100539.1.
UniGeneiRn.3902.

Genome annotation databases

EnsembliENSRNOT00000010593; ENSRNOP00000010593; ENSRNOG00000007967.
GeneIDi298596.
KEGGirno:298596.
UCSCiRGD:1308598. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473968 Genomic DNA. Translation: EDL80954.1.
BC158620 mRNA. Translation: AAI58621.1.
DQ403001 mRNA. Translation: ABD77134.1.
PIRiB32394.
RefSeqiNP_001094009.1. NM_001100539.1.
UniGeneiRn.3902.

3D structure databases

ProteinModelPortaliP21913.
SMRiP21913. Positions 39-277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP21913. 1 interaction.
STRINGi10116.ENSRNOP00000010593.

PTM databases

PhosphoSiteiP21913.

Proteomic databases

PaxDbiP21913.
PRIDEiP21913.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010593; ENSRNOP00000010593; ENSRNOG00000007967.
GeneIDi298596.
KEGGirno:298596.
UCSCiRGD:1308598. rat.

Organism-specific databases

CTDi6390.
RGDi1308598. Sdhb.

Phylogenomic databases

eggNOGiCOG0479.
GeneTreeiENSGT00390000013558.
HOGENOMiHOG000160590.
HOVERGENiHBG005483.
InParanoidiP21913.
KOiK00235.
OMAiIDSHERM.
OrthoDBiEOG7H4DTN.
PhylomeDBiP21913.
TreeFamiTF300754.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01006.
ReactomeiREACT_338796. Citric acid cycle (TCA cycle).
REACT_347303. Respiratory electron transport.
SABIO-RKP21913.

Miscellaneous databases

NextBioi644032.
PROiP21913.

Gene expression databases

GenevisibleiP21913. RN.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
[Graphical view]
PfamiPF13085. Fer2_3. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  3. "Housekeeping genes for phylogenetic analysis of eutherian relationships."
    Kullberg M., Nilsson M.A., Arnason U., Harley E.H., Janke A.
    Mol. Biol. Evol. 23:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-257.
    Tissue: Liver.
  4. "Use of the DNA polymerase chain reaction for homology probing: isolation of partial cDNA or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species."
    Gould S.J., Subramani S., Scheffler I.E.
    Proc. Natl. Acad. Sci. U.S.A. 86:1934-1938(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-251.
    Tissue: Brain cortex.
  5. Erratum
    Gould S.J., Subramani S., Scheffler I.E.
    Proc. Natl. Acad. Sci. U.S.A. 90:2556-2556(1993)

Entry informationi

Entry nameiSDHB_RAT
AccessioniPrimary (citable) accession number: P21913
Secondary accession number(s): B0BMZ2, Q0QEZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: September 2, 2008
Last modified: June 24, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.