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P21899 (RL40_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-60S ribosomal protein L40

Cleaved into the following 2 chains:

  1. Ubiquitin
  2. 60S ribosomal protein L40
    Alternative name(s):
    CEP52
OrganismTrypanosoma brucei brucei
Taxonomic identifier5702 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Protein attributes

Sequence length128 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ubiquitin: exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling By similarity.

60S ribosomal protein L40: component of the 60S subunit of the ribosome By similarity.

Subunit structure

Ribosomal protein L40 is part of the 60S ribosomal subunit By similarity.

Subcellular location

Ubiquitin: Cytoplasm By similarity. Nucleus By similarity.

60S ribosomal protein L40: Cytoplasm By similarity.

Sequence similarities

In the N-terminal section; belongs to the ubiquitin family.

In the C-terminal section; belongs to the ribosomal protein L40e family.

Contains 1 ubiquitin-like domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Molecular functionRibonucleoprotein
Ribosomal protein
   PTMIsopeptide bond
Ubl conjugation
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

ribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionstructural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7676Ubiquitin
PRO_0000114828
Chain77 – 1285260S ribosomal protein L40
PRO_0000138770

Regions

Domain1 – 7676Ubiquitin-like

Amino acid modifications

Cross-link48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-link76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) By similarity

Experimental info

Sequence conflict771V → L in CAA32691. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P21899 [UniParc].

Last modified August 10, 2010. Version 2.
Checksum: 3F617F6F62B156B6

FASTA12814,656
        10         20         30         40         50         60 
MQIFVKTLTG KTIALEVEAS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EEGRTLADYN 

        70         80         90        100        110        120 
IQKESTLHLV LRLRGGVMEP TLEALAKKYN WEKKVCRRCY ARLPVRATNC RKKGCGHCSN 


LRMKKKLR 

« Hide

References

[1]"Ubiquitin-EP52 fusion protein homologs from Trypanosoma brucei."
Wong S., Morales T.H., Campbell D.A.
Nucleic Acids Res. 18:7181-7181(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 427.
[2]"Genomic and transcriptional linkage of the genes for calmodulin, EF-hand 5 protein, and ubiquitin extension protein 52 in Trypanosoma brucei."
Wong S., Morales T.H., Neigel J.E., Campbell D.A.
Mol. Cell. Biol. 13:207-216(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 427.
[3]"A polyubiquitin gene from Trypanosoma brucei."
Wong S., Campbell D.A.
Mol. Biochem. Parasitol. 37:147-150(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54641 mRNA. Translation: CAA38453.1.
X54642 mRNA. Translation: CAA38454.1.
X56511 Genomic DNA. Translation: CAA39863.1.
X56511 Genomic DNA. Translation: CAA39864.1.
X14554 Genomic DNA. Translation: CAA32691.1.
PIRUQUT. A44981.
C48111.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZF7electron microscopy5.57s1-128[»]
ProteinModelPortalP21899.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001975. Ribosomal_L40e.
IPR011332. Ribosomal_zn-bd.
IPR019956. Ubiquitin.
IPR000626. Ubiquitin-like.
IPR019954. Ubiquitin_CS.
[Graphical view]
PfamPF01020. Ribosomal_L40e. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSPR00348. UBIQUITIN.
SMARTSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMSSF57829. SSF57829. 1 hit.
PROSITEPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRL40_TRYBB
AccessionPrimary (citable) accession number: P21899
Secondary accession number(s): P15174
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: August 10, 2010
Last modified: April 16, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references