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Protein

Aspartate--tRNA ligase

Gene

aspS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Asp) in a two-step reaction: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).UniRule annotation

Catalytic activityi

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171AspartateUniRule annotation1
Binding sitei217AspartateUniRule annotation1
Binding sitei226ATPUniRule annotation1
Binding sitei448AspartateUniRule annotation1
Binding sitei482ATPUniRule annotation1
Binding sitei489AspartateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi217 – 219ATPUniRule annotation3
Nucleotide bindingi534 – 537ATPUniRule annotation4

GO - Molecular functioni

  • aspartate-tRNA ligase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • aspartyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ASPS-MONOMER.
ECOL316407:JW1855-MONOMER.
MetaCyc:ASPS-MONOMER.
BRENDAi6.1.1.12. 2026.
SABIO-RKP21889.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate--tRNA ligaseUniRule annotation (EC:6.1.1.12UniRule annotation)
Alternative name(s):
Aspartyl-tRNA synthetaseUniRule annotation
Short name:
AspRSUniRule annotation
Gene namesi
Name:aspSUniRule annotation
Synonyms:tls
Ordered Locus Names:b1866, JW1855
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10097. aspS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001108681 – 590Aspartate--tRNA ligaseAdd BLAST590

Proteomic databases

EPDiP21889.
PaxDbiP21889.
PRIDEiP21889.

2D gel databases

SWISS-2DPAGEP21889.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4259330. 10 interactors.
DIPiDIP-9182N.
IntActiP21889. 26 interactors.
MINTiMINT-1249167.
STRINGi511145.b1866.

Chemistry databases

BindingDBiP21889.

Structurei

Secondary structure

1590
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 8Combined sources3
Helixi11 – 13Combined sources3
Beta strandi17 – 29Combined sources13
Beta strandi34 – 40Combined sources7
Beta strandi43 – 49Combined sources7
Helixi51 – 53Combined sources3
Helixi54 – 60Combined sources7
Beta strandi68 – 77Combined sources10
Turni80 – 82Combined sources3
Turni88 – 91Combined sources4
Beta strandi92 – 103Combined sources12
Beta strandi112 – 114Combined sources3
Helixi118 – 123Combined sources6
Helixi125 – 128Combined sources4
Helixi132 – 154Combined sources23
Beta strandi164 – 166Combined sources3
Beta strandi170 – 173Combined sources4
Beta strandi177 – 179Combined sources3
Beta strandi181 – 183Combined sources3
Beta strandi187 – 189Combined sources3
Helixi195 – 203Combined sources9
Beta strandi208 – 216Combined sources9
Beta strandi227 – 238Combined sources12
Helixi241 – 260Combined sources20
Beta strandi269 – 271Combined sources3
Helixi272 – 279Combined sources8
Beta strandi280 – 283Combined sources4
Beta strandi292 – 294Combined sources3
Helixi296 – 299Combined sources4
Helixi305 – 312Combined sources8
Beta strandi316 – 323Combined sources8
Helixi326 – 328Combined sources3
Helixi331 – 343Combined sources13
Beta strandi351 – 355Combined sources5
Helixi357 – 363Combined sources7
Helixi369 – 371Combined sources3
Helixi374 – 383Combined sources10
Beta strandi391 – 398Combined sources8
Helixi399 – 416Combined sources18
Beta strandi427 – 431Combined sources5
Beta strandi434 – 438Combined sources5
Beta strandi440 – 442Combined sources3
Beta strandi444 – 448Combined sources5
Beta strandi454 – 456Combined sources3
Helixi459 – 464Combined sources6
Turni466 – 468Combined sources3
Beta strandi470 – 478Combined sources9
Beta strandi481 – 489Combined sources9
Helixi493 – 502Combined sources10
Helixi507 – 520Combined sources14
Turni521 – 524Combined sources4
Beta strandi528 – 534Combined sources7
Helixi535 – 543Combined sources9
Helixi548 – 551Combined sources4
Beta strandi552 – 554Combined sources3
Turni563 – 566Combined sources4
Beta strandi567 – 570Combined sources4
Helixi573 – 578Combined sources6
Beta strandi581 – 583Combined sources3
Beta strandi586 – 588Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C0AX-ray2.40A1-585[»]
1EQRX-ray2.70A/B/C1-590[»]
1IL2X-ray2.60A/B1-590[»]
ProteinModelPortaliP21889.
SMRiP21889.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21889.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 198AspartateUniRule annotation4

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9M. Bacteria.
COG0173. LUCA.
HOGENOMiHOG000275159.
InParanoidiP21889.
KOiK01876.
OMAiYQLDVEM.
PhylomeDBiP21889.

Family and domain databases

Gene3Di3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth_type1. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_bac/mit.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 2 hits.
PTHR22594:SF5. PTHR22594:SF5. 2 hits.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTEYCGQLR LSHVGQQVTL CGWVNRRRDL GSLIFIDMRD REGIVQVFFD
60 70 80 90 100
PDRADALKLA SELRNEFCIQ VTGTVRARDE KNINRDMATG EIEVLASSLT
110 120 130 140 150
IINRADVLPL DSNHVNTEEA RLKYRYLDLR RPEMAQRLKT RAKITSLVRR
160 170 180 190 200
FMDDHGFLDI ETPMLTKATP EGARDYLVPS RVHKGKFYAL PQSPQLFKQL
210 220 230 240 250
LMMSGFDRYY QIVKCFRDED LRADRQPEFT QIDVETSFMT APQVREVMEA
260 270 280 290 300
LVRHLWLEVK GVDLGDFPVM TFAEAERRYG SDKPDLRNPM ELTDVADLLK
310 320 330 340 350
SVEFAVFAGP ANDPKGRVAA LRVPGGASLT RKQIDEYGNF VKIYGAKGLA
360 370 380 390 400
YIKVNERAKG LEGINSPVAK FLNAEIIEDI LDRTAAQDGD MIFFGADNKK
410 420 430 440 450
IVADAMGALR LKVGKDLGLT DESKWAPLWV IDFPMFEDDG EGGLTAMHHP
460 470 480 490 500
FTSPKDMTAA ELKAAPENAV ANAYDMVING YEVGGGSVRI HNGDMQQTVF
510 520 530 540 550
GILGINEEEQ REKFGFLLDA LKYGTPPHAG LAFGLDRLTM LLTGTDNIRD
560 570 580 590
VIAFPKTTAA ACLMTEAPSF ANPTALAELS IQVVKKAENN
Length:590
Mass (Da):65,913
Last modified:May 1, 1991 - v1
Checksum:iA411D3E16D3A9284
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53863 Genomic DNA. Translation: CAA37856.1.
U00096 Genomic DNA. Translation: AAC74936.1.
AP009048 Genomic DNA. Translation: BAA15677.1.
X53984 Genomic DNA. Translation: CAA37932.1.
D10165 Genomic DNA. No translation available.
PIRiJT0942. SYECD.
RefSeqiNP_416380.1. NC_000913.3.
WP_001258678.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74936; AAC74936; b1866.
BAA15677; BAA15677; BAA15677.
GeneIDi946385.
KEGGiecj:JW1855.
eco:b1866.
PATRICi32119055. VBIEscCol129921_1945.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53863 Genomic DNA. Translation: CAA37856.1.
U00096 Genomic DNA. Translation: AAC74936.1.
AP009048 Genomic DNA. Translation: BAA15677.1.
X53984 Genomic DNA. Translation: CAA37932.1.
D10165 Genomic DNA. No translation available.
PIRiJT0942. SYECD.
RefSeqiNP_416380.1. NC_000913.3.
WP_001258678.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C0AX-ray2.40A1-585[»]
1EQRX-ray2.70A/B/C1-590[»]
1IL2X-ray2.60A/B1-590[»]
ProteinModelPortaliP21889.
SMRiP21889.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259330. 10 interactors.
DIPiDIP-9182N.
IntActiP21889. 26 interactors.
MINTiMINT-1249167.
STRINGi511145.b1866.

Chemistry databases

BindingDBiP21889.

2D gel databases

SWISS-2DPAGEP21889.

Proteomic databases

EPDiP21889.
PaxDbiP21889.
PRIDEiP21889.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74936; AAC74936; b1866.
BAA15677; BAA15677; BAA15677.
GeneIDi946385.
KEGGiecj:JW1855.
eco:b1866.
PATRICi32119055. VBIEscCol129921_1945.

Organism-specific databases

EchoBASEiEB0095.
EcoGeneiEG10097. aspS.

Phylogenomic databases

eggNOGiENOG4105C9M. Bacteria.
COG0173. LUCA.
HOGENOMiHOG000275159.
InParanoidiP21889.
KOiK01876.
OMAiYQLDVEM.
PhylomeDBiP21889.

Enzyme and pathway databases

BioCyciEcoCyc:ASPS-MONOMER.
ECOL316407:JW1855-MONOMER.
MetaCyc:ASPS-MONOMER.
BRENDAi6.1.1.12. 2026.
SABIO-RKP21889.

Miscellaneous databases

EvolutionaryTraceiP21889.
PROiP21889.

Family and domain databases

Gene3Di3.30.1360.30. 1 hit.
HAMAPiMF_00044. Asp_tRNA_synth_type1. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-ligase_bac/mit.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD-like.
IPR029351. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 2 hits.
PTHR22594:SF5. PTHR22594:SF5. 2 hits.
PfamiPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsiTIGR00459. aspS_bact. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYD_ECOLI
AccessioniPrimary (citable) accession number: P21889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.