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Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28Zinc1
Metal bindingi209Zinc1
Metal bindingi234Zinc1
Metal bindingi238Zinc1
Binding sitei269ATPBy similarity1

GO - Molecular functioni

  • aminoacyl-tRNA ligase activity Source: EcoliWiki
  • ATP binding Source: EcoliWiki
  • cysteine-tRNA ligase activity Source: EcoliWiki
  • ligase activity Source: EcoliWiki
  • metal ion binding Source: EcoliWiki
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • cysteinyl-tRNA aminoacylation Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:CYSS-MONOMER.
ECOL316407:JW0515-MONOMER.
MetaCyc:CYSS-MONOMER.
BRENDAi6.1.1.16. 2026.
SABIO-RKP21888.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:cysS
Ordered Locus Names:b0526, JW0515
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10196. cysS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001593941 – 461Cysteine--tRNA ligaseAdd BLAST461

Proteomic databases

EPDiP21888.
PaxDbiP21888.
PRIDEiP21888.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262015. 12 interactors.
IntActiP21888. 3 interactors.
MINTiMINT-1240309.
STRINGi511145.b0526.

Structurei

Secondary structure

1461
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Turni7 – 9Combined sources3
Beta strandi10 – 14Combined sources5
Beta strandi22 – 27Combined sources6
Beta strandi31 – 34Combined sources4
Helixi38 – 57Combined sources20
Beta strandi60 – 65Combined sources6
Helixi72 – 80Combined sources9
Helixi85 – 102Combined sources18
Helixi114 – 116Combined sources3
Helixi118 – 130Combined sources13
Beta strandi133 – 136Combined sources4
Beta strandi142 – 144Combined sources3
Helixi146 – 148Combined sources3
Turni150 – 157Combined sources8
Helixi160 – 165Combined sources6
Beta strandi181 – 186Combined sources6
Beta strandi189 – 191Combined sources3
Beta strandi200 – 203Combined sources4
Helixi207 – 217Combined sources11
Beta strandi219 – 225Combined sources7
Helixi228 – 230Combined sources3
Turni231 – 233Combined sources3
Helixi234 – 245Combined sources12
Beta strandi246 – 248Combined sources3
Beta strandi251 – 254Combined sources4
Beta strandi260 – 262Combined sources3
Helixi269 – 271Combined sources3
Helixi277 – 281Combined sources5
Helixi286 – 294Combined sources9
Beta strandi302 – 304Combined sources3
Helixi306 – 323Combined sources18
Helixi336 – 347Combined sources12
Helixi352 – 372Combined sources21
Helixi374 – 388Combined sources15
Turni389 – 392Combined sources4
Helixi398 – 401Combined sources4
Beta strandi406 – 408Combined sources3
Helixi413 – 416Combined sources4
Helixi419 – 428Combined sources10
Helixi432 – 444Combined sources13
Beta strandi447 – 451Combined sources5
Beta strandi456 – 460Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LI5X-ray2.30A/B1-461[»]
1LI7X-ray2.60A/B1-461[»]
1U0BX-ray2.30B1-461[»]
ProteinModelPortaliP21888.
SMRiP21888.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21888.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi30 – 40"HIGH" regionAdd BLAST11
Motifi266 – 270"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245250.
InParanoidiP21888.
KOiK01883.
OMAiYQIDIHA.
PhylomeDBiP21888.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequencei

Sequence statusi: Complete.

P21888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKIFNTLTR QKEEFKPIHA GEVGMYVCGI TVYDLCHIGH GRTFVAFDVV
60 70 80 90 100
ARYLRFLGYK LKYVRNITDI DDKIIKRANE NGESFVAMVD RMIAEMHKDF
110 120 130 140 150
DALNILRPDM EPRATHHIAE IIELTEQLIA KGHAYVADNG DVMFDVPTDP
160 170 180 190 200
TYGVLSRQDL DQLQAGARVD VVDDKRNPMD FVLWKMSKEG EPSWPSPWGA
210 220 230 240 250
GRPGWHIECS AMNCKQLGNH FDIHGGGSDL MFPHHENEIA QSTCAHDGQY
260 270 280 290 300
VNYWMHSGMV MVDREKMSKS LGNFFTVRDV LKYYDAETVR YFLMSGHYRS
310 320 330 340 350
QLNYSEENLK QARAALERLY TALRGTDKTV APAGGEAFEA RFIEAMDDDF
360 370 380 390 400
NTPEAYSVLF DMAREVNRLK AEDMAAANAM ASHLRKLSAV LGLLEQEPEA
410 420 430 440 450
FLQSGAQADD SEVAEIEALI QQRLDARKAK DWAAADAARD RLNEMGIVLE
460
DGPQGTTWRR K
Length:461
Mass (Da):52,202
Last modified:May 1, 1992 - v2
Checksum:i2FA77FDBB7C5BA99
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti316L → V in CAA39691 (PubMed:2014166).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56234 Genomic DNA. Translation: CAA39691.1.
M59381 Genomic DNA. Translation: AAA23658.1.
X59293 Genomic DNA. Translation: CAA41983.1.
U82664 Genomic DNA. Translation: AAB40279.1.
U00096 Genomic DNA. Translation: AAC73628.1.
AP009048 Genomic DNA. Translation: BAE76303.1.
PIRiA37868. YYEC.
RefSeqiNP_415059.1. NC_000913.3.
WP_000912385.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73628; AAC73628; b0526.
BAE76303; BAE76303; BAE76303.
GeneIDi946969.
KEGGiecj:JW0515.
eco:b0526.
PATRICi32116212. VBIEscCol129921_0547.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56234 Genomic DNA. Translation: CAA39691.1.
M59381 Genomic DNA. Translation: AAA23658.1.
X59293 Genomic DNA. Translation: CAA41983.1.
U82664 Genomic DNA. Translation: AAB40279.1.
U00096 Genomic DNA. Translation: AAC73628.1.
AP009048 Genomic DNA. Translation: BAE76303.1.
PIRiA37868. YYEC.
RefSeqiNP_415059.1. NC_000913.3.
WP_000912385.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LI5X-ray2.30A/B1-461[»]
1LI7X-ray2.60A/B1-461[»]
1U0BX-ray2.30B1-461[»]
ProteinModelPortaliP21888.
SMRiP21888.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262015. 12 interactors.
IntActiP21888. 3 interactors.
MINTiMINT-1240309.
STRINGi511145.b0526.

Proteomic databases

EPDiP21888.
PaxDbiP21888.
PRIDEiP21888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73628; AAC73628; b0526.
BAE76303; BAE76303; BAE76303.
GeneIDi946969.
KEGGiecj:JW0515.
eco:b0526.
PATRICi32116212. VBIEscCol129921_0547.

Organism-specific databases

EchoBASEiEB0193.
EcoGeneiEG10196. cysS.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245250.
InParanoidiP21888.
KOiK01883.
OMAiYQIDIHA.
PhylomeDBiP21888.

Enzyme and pathway databases

BioCyciEcoCyc:CYSS-MONOMER.
ECOL316407:JW0515-MONOMER.
MetaCyc:CYSS-MONOMER.
BRENDAi6.1.1.16. 2026.
SABIO-RKP21888.

Miscellaneous databases

EvolutionaryTraceiP21888.
PROiP21888.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SMARTiSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYC_ECOLI
AccessioniPrimary (citable) accession number: P21888
Secondary accession number(s): Q2MBQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1992
Last modified: November 2, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.