Reviewed, UniProtKB/Swiss-Prot P21885 (SPEA_BACSU)

Names and origin

Protein names

Recommended name:
    Arginine decarboxylase
    EC=4.1.1.19

Gene names

Name:	speA
Synonyms:	cad
Ordered Locus Names:	BSU14630

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of agmatine from arginine.
Catalytic activity	L-arginine = agmatine + CO2.
Cofactor	Pyridoxal phosphate.
Pathway	Amine and polyamine biosynthesis; agmatine biosynthesis; agmatine from L-arginine: step 1/1. Ref.6
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the Orn/Lys/Arg decarboxylase class-I family.
Caution	Was originally (Ref.1) thought to be a lysine decarboxylase and was consequently named cad.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Putrescine biosynthesis Spermidine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	putrescine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW spermidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arginine decarboxylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Arginine decarboxylase		PRO_0000201147
Amino acid modifications
	Modified residue	226	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21885-1 [UniParc]. Last modified December 1, 1992. Version 2. Checksum: 1AC7ACAE24F4F6D2	FASTA	490	53,583
	10 20 30 40 50 60 MSQHETPLYT GLKKHASRQP VQFHIPGHKK GAGMDPEFRQ FIGENALSID LINIEPLDDL 70 80 90 100 110 120 HAPKGIIKQA QDLAAEAFGA DHTFFSVQGT SGAIMTMVMA VCGPGDKIII PRNVHKSIMT 130 140 150 160 170 180 AIVFSGAVPI FIHPEIDNEL GISHGITLES AKRALTEHPD AKGLLVINPT YFGVAADLKS 190 200 210 220 230 240 IVELAHSFDV PVLVDEAHGV HIHFHDELPL SAMQAGADIA ATSVHKLGGS LTQSSILNMR 250 260 270 280 290 300 EGLVSKDRVQ SILSMLTTTS TSYLLLASLD VARKRLATEG RQLAEETLKL ANQTRDRLNQ 310 320 330 340 350 360 IEGIYCVGSE ILGSKAAYSY DPTKLIISVK SLGLTGHDVE KWLRESFNIE VELSDLYNIL 370 380 390 400 410 420 CIFTPGDSQN DADRLVEALT EIAQQMSEQD VTHQQTEVLL PEIPLLAMTP RDAFYANTEV 430 440 450 460 470 480 IPLKEASGRI IAEFVMVYPP GIPIFIPGEI ITEENISYIF KNLDAGLPVQ GPEDSTLHMI 490 RVIKEQKAIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing the lysine decarboxylase of Bacillus subtilis." Hemilae H., Koivula T., Paulin L. Submitted (MAR-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area." Winters P., Caldwell R.M., Enfield L., Ferrari E. Microbiology 142:3033-3037(1996) [PubMed: 8969500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Secretory S complex of Bacillus subtilis: sequence analysis and identity to pyruvate dehydrogenase." Hemilae H.O., Palva A., Paulin L., Arvidson S., Palva I. J. Bacteriol. 172:5052-5063(1990) [PubMed: 1697575] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 34-490. Strain: 168.
[5]	"Sequence of a PAL-related lipoprotein from Bacillus subtilis." Hemilae H.O. FEMS Microbiol. Lett. 66:37-41(1991) [PubMed: 1936936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 485-490.
[6]	"Characterization of polyamine synthesis pathway in Bacillus subtilis 168." Sekowska A., Bertin P., Danchin A. Mol. Microbiol. 29:851-858(1998) [PubMed: 9723923] [Abstract] Cited for: CHARACTERIZATION OF POLYAMINE PATHWAY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X58433 Genomic DNA. Translation: CAA41337.1. AF012285 Genomic DNA. Translation: AAC24937.1. AL009126 Genomic DNA. Translation: CAB13336.1. M57435 Genomic DNA. Translation: AAA62686.1.
PIR	A54546.
RefSeq	NP_389346.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	936002.
GenomeReviews	Gene locus BSU14630 in contig AL009126_GR.
KEGG	bsu:BSU14630.
NMPDR	fig|224308.1.peg.1465.
Organism-specific databases
SubtiList	BG10212. speA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P21885.
OMA	FKYVEEG.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1465-MON.
BRENDA	4.1.1.19. 150.
Family and domain databases
InterPro	IPR000310. Decarbxylse1. IPR008286. Prn/Lys/Arg_de-COase_C. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.90.100.10. Decarbxylse_C. 1 hit. G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF01276. OKR_DC_1. 1 hit. PF03711. OKR_DC_1_C. 1 hit. [Graphical view]
PROSITE	PS00703. OKR_DC_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEA_BACSU

Accession

Primary (citable) accession number: P21885
Secondary accession number(s): P26934

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	December 1, 1992
Last modified:	November 3, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)