Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P21881

- ODPA_BACSU

UniProt

P21881 - ODPA_BACSU

Protein

Pyruvate dehydrogenase E1 component subunit alpha

Gene

pdhA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
    The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity.

    Catalytic activityi

    Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

    Cofactori

    Thiamine pyrophosphate.

    GO - Molecular functioni

    1. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC
    2. thiamine pyrophosphate binding Source: InterPro

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Pyruvate, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciBSUB:BSU14580-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate dehydrogenase E1 component subunit alpha (EC:1.2.4.1)
    Alternative name(s):
    S complex, 42 kDa subunit
    Vegetative protein 220
    Short name:
    VEG220
    Gene namesi
    Name:pdhA
    Synonyms:aceA
    Ordered Locus Names:BSU14580
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU14580. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 371370Pyruvate dehydrogenase E1 component subunit alphaPRO_0000162199Add
    BLAST

    Proteomic databases

    PaxDbiP21881.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta chain.

    Protein-protein interaction databases

    IntActiP21881. 1 interaction.
    MINTiMINT-8365401.
    STRINGi224308.BSU14580.

    Structurei

    3D structure databases

    ProteinModelPortaliP21881.
    SMRiP21881. Positions 14-371.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG1071.
    HOGENOMiHOG000281335.
    KOiK00161.
    OMAiEAYIEEA.
    OrthoDBiEOG6VMTKR.
    PhylomeDBiP21881.

    Family and domain databases

    Gene3Di3.40.50.970. 1 hit.
    InterProiIPR001017. DH_E1.
    IPR017596. PdhA/BkdA.
    IPR029061. THDP-binding.
    [Graphical view]
    PfamiPF00676. E1_dh. 1 hit.
    [Graphical view]
    SUPFAMiSSF52518. SSF52518. 1 hit.
    TIGRFAMsiTIGR03181. PDH_E1_alph_x. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21881-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAKTKKAIV DSKKQFDAIK KQFETFQILN EKGEVVNEAA MPDLTDDQLK    50
    ELMRRMVFTR VLDQRSISLN RQGRLGFYAP TAGQEASQIA THFALEKEDF 100
    VLPGYRDVPQ LIWHGLPLYQ AFLFSRGHFR GNQMPDDVNA LSPQIIIGAQ 150
    YIQTAGVALG LKKRGKKAVA ITYTGDGGAS QGDFYEGINF AGAYKAPAIF 200
    VVQNNRYAIS TPVEKQSAAE TIAQKAVAAG IVGVQVDGMD PLAVYAATAE 250
    ARERAINGEG PTLIETLTFR YGPHTMAGDD PTKYRTKEIE NEWEQKDPLV 300
    RFRAFLENKG LWSEEEEAKV IEDAKEEIKQ AIKKADAEPK QKVTDLMKIM 350
    YEKMPHNLEE QFEIYTQKES K 371
    Length:371
    Mass (Da):41,548
    Last modified:January 23, 2007 - v3
    Checksum:i984AE665278C1462
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti179 – 1791A → R in AAA62681. (PubMed:1697575)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57435 Genomic DNA. Translation: AAA62681.1.
    AF012285 Genomic DNA. Translation: AAC24932.1.
    AL009126 Genomic DNA. Translation: CAB13331.1.
    PIRiB36718. DEBSPA.
    RefSeqiNP_389341.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB13331; CAB13331; BSU14580.
    GeneIDi936005.
    KEGGibsu:BSU14580.
    PATRICi18974709. VBIBacSub10457_1546.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M57435 Genomic DNA. Translation: AAA62681.1 .
    AF012285 Genomic DNA. Translation: AAC24932.1 .
    AL009126 Genomic DNA. Translation: CAB13331.1 .
    PIRi B36718. DEBSPA.
    RefSeqi NP_389341.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P21881.
    SMRi P21881. Positions 14-371.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P21881. 1 interaction.
    MINTi MINT-8365401.
    STRINGi 224308.BSU14580.

    Proteomic databases

    PaxDbi P21881.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB13331 ; CAB13331 ; BSU14580 .
    GeneIDi 936005.
    KEGGi bsu:BSU14580.
    PATRICi 18974709. VBIBacSub10457_1546.

    Organism-specific databases

    GenoListi BSU14580. [Micado ]

    Phylogenomic databases

    eggNOGi COG1071.
    HOGENOMi HOG000281335.
    KOi K00161.
    OMAi EAYIEEA.
    OrthoDBi EOG6VMTKR.
    PhylomeDBi P21881.

    Enzyme and pathway databases

    BioCyci BSUB:BSU14580-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.970. 1 hit.
    InterProi IPR001017. DH_E1.
    IPR017596. PdhA/BkdA.
    IPR029061. THDP-binding.
    [Graphical view ]
    Pfami PF00676. E1_dh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52518. SSF52518. 1 hit.
    TIGRFAMsi TIGR03181. PDH_E1_alph_x. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Secretory S complex of Bacillus subtilis: sequence analysis and identity to pyruvate dehydrogenase."
      Hemilae H.O., Palva A., Paulin L., Arvidson S., Palva I.
      J. Bacteriol. 172:5052-5063(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area."
      Winters P., Caldwell R.M., Enfield L., Ferrari E.
      Microbiology 142:3033-3037(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "Sequence analysis of the mobA-ampS region of the Bacillus subtilis chromosome."
      Caldwell R.M., Ferrari E.
      Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    5. "First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis."
      Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M.
      Electrophoresis 18:1451-1463(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-16.
      Strain: 168 / IS58.

    Entry informationi

    Entry nameiODPA_BACSU
    AccessioniPrimary (citable) accession number: P21881
    Secondary accession number(s): Q59227
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 111 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

    External Data

    Dasty 3