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P21881

- ODPA_BACSU

UniProt

P21881 - ODPA_BACSU

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Protein

Pyruvate dehydrogenase E1 component subunit alpha

Gene

pdhA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity.

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

GO - Molecular functioni

  1. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Pyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciBSUB:BSU14580-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit alpha (EC:1.2.4.1)
Alternative name(s):
S complex, 42 kDa subunit
Vegetative protein 220
Short name:
VEG220
Gene namesi
Name:pdhA
Synonyms:aceA
Ordered Locus Names:BSU14580
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU14580. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 371370Pyruvate dehydrogenase E1 component subunit alphaPRO_0000162199Add
BLAST

Proteomic databases

PaxDbiP21881.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

IntActiP21881. 1 interaction.
MINTiMINT-8365401.
STRINGi224308.BSU14580.

Structurei

3D structure databases

ProteinModelPortaliP21881.
SMRiP21881. Positions 14-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG1071.
HOGENOMiHOG000281335.
InParanoidiP21881.
KOiK00161.
OMAiEAYIEEA.
OrthoDBiEOG6VMTKR.
PhylomeDBiP21881.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR017596. PdhA/BkdA.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
TIGRFAMsiTIGR03181. PDH_E1_alph_x. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21881-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAKTKKAIV DSKKQFDAIK KQFETFQILN EKGEVVNEAA MPDLTDDQLK
60 70 80 90 100
ELMRRMVFTR VLDQRSISLN RQGRLGFYAP TAGQEASQIA THFALEKEDF
110 120 130 140 150
VLPGYRDVPQ LIWHGLPLYQ AFLFSRGHFR GNQMPDDVNA LSPQIIIGAQ
160 170 180 190 200
YIQTAGVALG LKKRGKKAVA ITYTGDGGAS QGDFYEGINF AGAYKAPAIF
210 220 230 240 250
VVQNNRYAIS TPVEKQSAAE TIAQKAVAAG IVGVQVDGMD PLAVYAATAE
260 270 280 290 300
ARERAINGEG PTLIETLTFR YGPHTMAGDD PTKYRTKEIE NEWEQKDPLV
310 320 330 340 350
RFRAFLENKG LWSEEEEAKV IEDAKEEIKQ AIKKADAEPK QKVTDLMKIM
360 370
YEKMPHNLEE QFEIYTQKES K
Length:371
Mass (Da):41,548
Last modified:January 23, 2007 - v3
Checksum:i984AE665278C1462
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti179 – 1791A → R in AAA62681. (PubMed:1697575)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57435 Genomic DNA. Translation: AAA62681.1.
AF012285 Genomic DNA. Translation: AAC24932.1.
AL009126 Genomic DNA. Translation: CAB13331.1.
PIRiB36718. DEBSPA.
RefSeqiNP_389341.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB13331; CAB13331; BSU14580.
GeneIDi936005.
KEGGibsu:BSU14580.
PATRICi18974709. VBIBacSub10457_1546.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57435 Genomic DNA. Translation: AAA62681.1 .
AF012285 Genomic DNA. Translation: AAC24932.1 .
AL009126 Genomic DNA. Translation: CAB13331.1 .
PIRi B36718. DEBSPA.
RefSeqi NP_389341.1. NC_000964.3.

3D structure databases

ProteinModelPortali P21881.
SMRi P21881. Positions 14-371.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P21881. 1 interaction.
MINTi MINT-8365401.
STRINGi 224308.BSU14580.

Proteomic databases

PaxDbi P21881.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13331 ; CAB13331 ; BSU14580 .
GeneIDi 936005.
KEGGi bsu:BSU14580.
PATRICi 18974709. VBIBacSub10457_1546.

Organism-specific databases

GenoListi BSU14580. [Micado ]

Phylogenomic databases

eggNOGi COG1071.
HOGENOMi HOG000281335.
InParanoidi P21881.
KOi K00161.
OMAi EAYIEEA.
OrthoDBi EOG6VMTKR.
PhylomeDBi P21881.

Enzyme and pathway databases

BioCyci BSUB:BSU14580-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 1 hit.
InterProi IPR001017. DH_E1.
IPR017596. PdhA/BkdA.
IPR029061. THDP-binding.
[Graphical view ]
Pfami PF00676. E1_dh. 1 hit.
[Graphical view ]
SUPFAMi SSF52518. SSF52518. 1 hit.
TIGRFAMsi TIGR03181. PDH_E1_alph_x. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Secretory S complex of Bacillus subtilis: sequence analysis and identity to pyruvate dehydrogenase."
    Hemilae H.O., Palva A., Paulin L., Arvidson S., Palva I.
    J. Bacteriol. 172:5052-5063(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area."
    Winters P., Caldwell R.M., Enfield L., Ferrari E.
    Microbiology 142:3033-3037(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "Sequence analysis of the mobA-ampS region of the Bacillus subtilis chromosome."
    Caldwell R.M., Ferrari E.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. "First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis."
    Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M.
    Electrophoresis 18:1451-1463(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16.
    Strain: 168 / IS58.

Entry informationi

Entry nameiODPA_BACSU
AccessioniPrimary (citable) accession number: P21881
Secondary accession number(s): Q59227
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

External Data

Dasty 3