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P21874

- ODPB_GEOSE

UniProt

P21874 - ODPB_GEOSE

Protein

Pyruvate dehydrogenase E1 component subunit beta

Gene

pdhB

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

    Catalytic activityi

    Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

    Cofactori

    Thiamine pyrophosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei60 – 601Thiamine pyrophosphateBy similarity

    GO - Molecular functioni

    1. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Pyruvate, Thiamine pyrophosphate

    Enzyme and pathway databases

    SABIO-RKP21874.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate dehydrogenase E1 component subunit beta (EC:1.2.4.1)
    Gene namesi
    Name:pdhB
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 325324Pyruvate dehydrogenase E1 component subunit betaPRO_0000162221Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta chain.

    Protein-protein interaction databases

    DIPiDIP-29597N.
    IntActiP21874. 1 interaction.

    Structurei

    Secondary structure

    1
    325
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 53
    Helixi6 – 2015
    Beta strandi24 – 285
    Beta strandi32 – 343
    Turni40 – 434
    Helixi44 – 485
    Turni50 – 523
    Beta strandi53 – 553
    Helixi60 – 7213
    Beta strandi76 – 805
    Helixi84 – 896
    Helixi91 – 955
    Helixi98 – 1003
    Helixi101 – 1044
    Turni105 – 1073
    Beta strandi114 – 1196
    Beta strandi121 – 1233
    Turni127 – 1293
    Helixi134 – 1374
    Beta strandi144 – 1463
    Helixi151 – 16313
    Beta strandi164 – 1663
    Beta strandi168 – 1736
    Turni174 – 1763
    Beta strandi177 – 1793
    Beta strandi181 – 1833
    Beta strandi197 – 2004
    Beta strandi203 – 2097
    Helixi213 – 22614
    Beta strandi231 – 2355
    Beta strandi237 – 2415
    Helixi244 – 25411
    Beta strandi257 – 2648
    Beta strandi267 – 2693
    Helixi270 – 28112
    Helixi282 – 2843
    Beta strandi290 – 2945
    Beta strandi296 – 2994
    Helixi302 – 3043
    Helixi305 – 3084
    Helixi312 – 32312

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1W85X-ray2.00B/D/F/H2-325[»]
    1W88X-ray2.30B/D/F/H2-325[»]
    3DUFX-ray2.50B/D/F/H1-325[»]
    3DV0X-ray2.50B/D/F/H1-325[»]
    3DVAX-ray2.35B/D/F/H1-325[»]
    ProteinModelPortaliP21874.
    SMRiP21874. Positions 2-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21874.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.50.920. 1 hit.
    3.40.50.970. 1 hit.
    InterProiIPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005475. Transketolase-like_Pyr-bd.
    IPR005476. Transketolase_C.
    [Graphical view]
    PfamiPF02779. Transket_pyr. 1 hit.
    PF02780. Transketolase_C. 1 hit.
    [Graphical view]
    SMARTiSM00861. Transket_pyr. 1 hit.
    [Graphical view]
    SUPFAMiSSF52518. SSF52518. 1 hit.
    SSF52922. SSF52922. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21874-1 [UniParc]FASTAAdd to Basket

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    MAQMTMVQAI TDALRIELKN DPNVLIFGED VGVNGGVFRA TEGLQAEFGE    50
    DRVFDTPLAE SGIGGLAIGL ALQGFRPVPE IQFFGFVYEV MDSICGQMAR 100
    IRYRTGGRYH MPITIRSPFG GGVHTPELHS DSLEGLVAQQ PGLKVVIPST 150
    PYDAKGLLIS AIRDNDPVIF LEHLKLYRSF RQEVPEGEYT IPIGKADIKR 200
    EGKDITIIAY GAMVHESLKA AAELEKEGIS AEVVDLRTVQ PLDIETIIGS 250
    VEKTGRAIVV QEAQRQAGIA ANVVAEINER AILSLEAPVL RVAAPDTVYP 300
    FAQAESVWLP NFKDVIETAK KVMNF 325
    Length:325
    Mass (Da):35,460
    Last modified:January 23, 2007 - v2
    Checksum:iAD1F2D4F82D652AC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53560 Genomic DNA. Translation: CAA37629.1.
    PIRiS14230.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53560 Genomic DNA. Translation: CAA37629.1 .
    PIRi S14230.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1W85 X-ray 2.00 B/D/F/H 2-325 [» ]
    1W88 X-ray 2.30 B/D/F/H 2-325 [» ]
    3DUF X-ray 2.50 B/D/F/H 1-325 [» ]
    3DV0 X-ray 2.50 B/D/F/H 1-325 [» ]
    3DVA X-ray 2.35 B/D/F/H 1-325 [» ]
    ProteinModelPortali P21874.
    SMRi P21874. Positions 2-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29597N.
    IntActi P21874. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P21874.

    Miscellaneous databases

    EvolutionaryTracei P21874.

    Family and domain databases

    Gene3Di 3.40.50.920. 1 hit.
    3.40.50.970. 1 hit.
    InterProi IPR029061. THDP-binding.
    IPR009014. Transketo_C/Pyr-ferredox_oxred.
    IPR005475. Transketolase-like_Pyr-bd.
    IPR005476. Transketolase_C.
    [Graphical view ]
    Pfami PF02779. Transket_pyr. 1 hit.
    PF02780. Transketolase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00861. Transket_pyr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52518. SSF52518. 1 hit.
    SSF52922. SSF52922. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of the genes encoding the alpha and beta subunits of the E1 component of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus."
      Hawkins C.F., Borges A., Perham R.N.
      Eur. J. Biochem. 191:337-346(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.
    2. "Cloning and sequence analysis of the genes encoding the dihydrolipoamide acetyltransferase and dihydrolipoamide dehydrogenase components of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus."
      Borges A., Hawkins C.F., Packman L.C., Perham R.N.
      Eur. J. Biochem. 194:95-102(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiODPB_GEOSE
    AccessioniPrimary (citable) accession number: P21874
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3