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P21873

- ODPA_GEOSE

UniProt

P21873 - ODPA_GEOSE

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Protein

Pyruvate dehydrogenase E1 component subunit alpha

Gene

pdhA

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.

Cofactori

Thiamine pyrophosphate.

GO - Molecular functioni

  1. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Pyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

SABIO-RKP21873.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit alpha (EC:1.2.4.1)
Gene namesi
Name:pdhA
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 369368Pyruvate dehydrogenase E1 component subunit alphaPRO_0000162198Add
BLAST

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

DIPiDIP-29596N.
IntActiP21873. 1 interaction.

Structurei

Secondary structure

1
369
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 1910
Helixi36 – 383
Helixi44 – 6926
Helixi83 – 919
Beta strandi98 – 1003
Beta strandi102 – 1043
Helixi106 – 1116
Helixi116 – 1249
Helixi127 – 1304
Helixi147 – 16115
Beta strandi168 – 1736
Helixi175 – 1784
Helixi180 – 19112
Beta strandi196 – 2027
Beta strandi204 – 2063
Helixi211 – 2133
Helixi221 – 2266
Beta strandi231 – 2355
Helixi239 – 25416
Beta strandi260 – 2656
Beta strandi274 – 2774
Helixi278 – 2803
Helixi285 – 2928
Helixi296 – 30611
Helixi312 – 33423
Helixi341 – 3466
Helixi354 – 36613

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W85X-ray2.00A/C/E/G2-369[»]
1W88X-ray2.30A/C/E/G2-369[»]
3DUFX-ray2.50A/C/E/G1-369[»]
3DV0X-ray2.50A/C/E/G1-369[»]
3DVAX-ray2.35A/C/E/G1-369[»]
ProteinModelPortaliP21873.
SMRiP21873. Positions 5-369.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21873.

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR017596. PdhA/BkdA.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
TIGRFAMsiTIGR03181. PDH_E1_alph_x. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21873 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGVKTFQFPF AEQLEKVAEQ FPTFQILNEE GEVVNEEAMP ELSDEQLKEL
60 70 80 90 100
MRRMVYTRIL DQRSISLNRQ GRLGFYAPTA GQEASQIASH FALEKEDFIL
110 120 130 140 150
PGYRDVPQII WHGLPLYQAF LFSRGHFHGN QIPEGVNVLP PQIIIGAQYI
160 170 180 190 200
QAAGVALGLK MRGKKAVAIT YTGDGGTSQG DFYEGINFAG AFKAPAIFVV
210 220 230 240 250
QNNRFAISTP VEKQTVAKTL AQKAVAAGIP GIQVDGMDPL AVYAAVKAAR
260 270 280 290 300
ERAINGEGPT LIETLCFRYG PHTMSGDDPT RYRSKELENE WAKKDPLVRF
310 320 330 340 350
RKFLEAKGLW SEEEENNVIE QAKEEIKEAI KKADETPKQK VTDLISIMFE
360
ELPFNLKEQY EIYKEKESK
Length:369
Mass (Da):41,469
Last modified:January 23, 2007 - v2
Checksum:iDBF43982C0E1926D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53560 Genomic DNA. Translation: CAA37628.1.
PIRiS10798. DEBSPF.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53560 Genomic DNA. Translation: CAA37628.1 .
PIRi S10798. DEBSPF.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1W85 X-ray 2.00 A/C/E/G 2-369 [» ]
1W88 X-ray 2.30 A/C/E/G 2-369 [» ]
3DUF X-ray 2.50 A/C/E/G 1-369 [» ]
3DV0 X-ray 2.50 A/C/E/G 1-369 [» ]
3DVA X-ray 2.35 A/C/E/G 1-369 [» ]
ProteinModelPortali P21873.
SMRi P21873. Positions 5-369.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-29596N.
IntActi P21873. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P21873.

Miscellaneous databases

EvolutionaryTracei P21873.

Family and domain databases

Gene3Di 3.40.50.970. 1 hit.
InterProi IPR001017. DH_E1.
IPR017596. PdhA/BkdA.
IPR029061. THDP-binding.
[Graphical view ]
Pfami PF00676. E1_dh. 1 hit.
[Graphical view ]
SUPFAMi SSF52518. SSF52518. 1 hit.
TIGRFAMsi TIGR03181. PDH_E1_alph_x. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequence analysis of the genes encoding the alpha and beta subunits of the E1 component of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus."
    Hawkins C.F., Borges A., Perham R.N.
    Eur. J. Biochem. 191:337-346(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-35; 54-111 AND 269-331.
    Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.

Entry informationi

Entry nameiODPA_GEOSE
AccessioniPrimary (citable) accession number: P21873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3