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P21872 (PUR2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Trifunctional purine biosynthetic protein adenosine-3

Including the following 3 domains:

  1. Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name=GARS
    Phosphoribosylglycinamide synthetase
  2. Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
    Alternative name(s):
    AIR synthase
    Short name=AIRS
    Phosphoribosyl-aminoimidazole synthetase
  3. Phosphoribosylglycinamide formyltransferase
    EC=2.1.2.2
    Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
    Short name=GART
Gene names
Name:GART
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length1003 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.

Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.

Sequence similarities

In the N-terminal section; belongs to the GARS family.

In the central section; belongs to the AIR synthase family.

In the C-terminal section; belongs to the GART family.

Contains 1 ATP-grasp domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P21872-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P21872-2)

The sequence of this isoform differs from the canonical sequence as follows:
     434-1003: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10031003Trifunctional purine biosynthetic protein adenosine-3
PRO_0000074936

Regions

Domain111 – 318208ATP-grasp
Nucleotide binding137 – 19963ATP By similarity
Region434 – 805372AIRS
Region806 – 1003198GART
Region814 – 81635'-phosphoribosylglycinamide binding By similarity
Region892 – 895410-formyltetrahydrofolate binding By similarity
Region943 – 947510-formyltetrahydrofolate binding By similarity
Region973 – 97645'-phosphoribosylglycinamide binding By similarity

Sites

Active site9111Proton donor By similarity
Metal binding2881Manganese By similarity
Metal binding2901Manganese By similarity
Binding site867110-formyltetrahydrofolate By similarity
Binding site909110-formyltetrahydrofolate By similarity
Site9471Raises pKa of active site His By similarity

Natural variations

Alternative sequence434 – 1003570Missing in isoform Short.
VSP_005516

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: A0C66BA0EBF791DE

FASTA1,003106,544
        10         20         30         40         50         60 
MADRVLVIGS GGREHALAWK LAQSPHVKQV FVAPGNAGTA NSGKISNSAV SVSNHAALAQ 

        70         80         90        100        110        120 
FCRDQEIRLV VVGPEVPLAA GIVDDLTAAG VRCFGPTARA AQLESSKSFT KSFLDRHGIP 

       130        140        150        160        170        180 
TARWKSFTDP KAACSFINSA NFPALVVKAS GLAAGKGVIV ASNKEEACKA VNDIMQDKTF 

       190        200        210        220        230        240 
GTAGETVVVE ELLEGEEVSC LCFTDGVTIA PMPPAQDHKR LKDGDEGPNT GGMGAYSPAP 

       250        260        270        280        290        300 
QISKDLLLKI RETVLQKTLD GMRKEGIPYL GVLYAGLMLT KDGPKVLEFN CRFGDPECQV 

       310        320        330        340        350        360 
ILPLLKSDLY EVMQAVINKK LSSSMPIWYE DSAAVTVVMA SEGYPGTYPK GLEITGLSKA 

       370        380        390        400        410        420 
KELGLEVFHA GTALKDGKVV TNGGRVLTVT AIKEDLMTAL QEANKGVAAI NFKGSIYRKD 

       430        440        450        460        470        480 
IGYRAIAFLS QSRGLTYKNS GVDIAAGNIL VQKIKPLAAA TSRSGCNAEL GGFAGLFDLK 

       490        500        510        520        530        540 
AAGYKDPILV SGTDGVGTKL KIAQVCKKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFAC 

       550        560        570        580        590        600 
GKLDVEVAQG VIAGIAEACQ KAGCALLGGE TAEMPGMYPP GEYDLAGFAV GAVERGQMLP 

       610        620        630        640        650        660 
QLERIADGDV VIGVASSGVH SNGYSLVRRI VEKSSLDFSS QVGVSGDQTL GDLLLTPTKI 

       670        680        690        700        710        720 
YSKTLLPVLR SGHVKAYAHI TGGGLLENIP RVLPDSFGVV LDALSWKIPE IFCWLHKEGN 

       730        740        750        760        770        780 
LSEEEMARTF NCGIGAVLVV QKELAQQVLK DVQKHEAAWL IGKVVPLQKG SAHVKVHNLL 

       790        800        810        820        830        840 
QALQANRSLS VHSHIQGKIQ TNKVKVAVLI SGTGTNLEAL INSTKKPTSF AEIVLVVSNK 

       850        860        870        880        890        900 
AGVEGLRKAE RAGIPTRVID HKQYGSRTEF DSAVDRVLEE FSVELICLAG FMRILSGPFV 

       910        920        930        940        950        960 
KKWEGKILNI HPSLLPSFKG ANAHKLVLEA GVRVTGCTVH FVAEEVDAGA IIFQEAVPVK 

       970        980        990       1000 
IGDTVETLSE RVKEAEHRAF PAALQLVASG AVQVGEAGKI CWK

« Hide

Isoform Short [UniParc].

Checksum: C110705BF612798F
Show »

FASTA43345,990

References

[1]"Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli."
Aimi J., Badylak J., Williams J., Chen Z., Zalkin H., Dixon J.E.
J. Biol. Chem. 265:9011-9014(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli."
Aimi J., Qiu H., Williams J., Zalkin H., Dixon J.E.
Nucleic Acids Res. 18:6665-6672(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X56339 mRNA. Translation: CAA39779.1.
X54200 mRNA. Translation: CAA38120.1.
IPIIPI00576778.
IPI00823188.
PIRAJCHPR. S12617.
RefSeqNP_001001469.1. NM_001001469.1.
UniGeneGga.4704.

3D structure databases

ProteinModelPortalP21872.
SMRP21872. Positions 1-429, 434-789, 803-1002.
ModBaseSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000025711.

Proteomic databases

PaxDbP21872.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID395315.
KEGGgga:395315.

Organism-specific databases

CTD2618.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000030315.
HOVERGENHBG008333.
KOK11787.
OrthoDBEOG4FFD13.

Enzyme and pathway databases

ReactomeREACT_115655. Metabolism.
UniPathwayUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 1 hit.
SSF53328. formyl_transf. 1 hit.
SSF52440. PreATP-grasp-like. 1 hit.
SSF55326. PurM_N-like. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20815400.

Entry information

Entry namePUR2_CHICK
AccessionPrimary (citable) accession number: P21872
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: May 29, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents