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P21872

- PUR2_CHICK

UniProt

P21872 - PUR2_CHICK

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Protein
Trifunctional purine biosynthetic protein adenosine-3
Gene
GART
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi288 – 2881Manganese By similarity
Metal bindingi290 – 2901Manganese By similarity
Binding sitei867 – 867110-formyltetrahydrofolate By similarity
Binding sitei909 – 909110-formyltetrahydrofolate By similarity
Active sitei911 – 9111Proton donor By similarity
Sitei947 – 9471Raises pKa of active site His By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 19963ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. methyltransferase activity Source: InterPro
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
  5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC
  6. phosphoribosylglycinamide formyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: Reactome
  2. nucleobase-containing small molecule metabolic process Source: Reactome
  3. purine nucleobase biosynthetic process Source: InterPro
  4. purine nucleobase metabolic process Source: Reactome
  5. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_116007. De novo synthesis of IMP.
UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:GART
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10031003Trifunctional purine biosynthetic protein adenosine-3UniRule annotation
PRO_0000074936Add
BLAST

Proteomic databases

PaxDbiP21872.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000025711.

Structurei

3D structure databases

ProteinModelPortaliP21872.
SMRiP21872. Positions 1-429, 434-789, 803-1002.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 318208ATP-grasp
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni434 – 805372AIRSUniRule annotation
Add
BLAST
Regioni806 – 1003198GARTUniRule annotation
Add
BLAST
Regioni814 – 81635'-phosphoribosylglycinamide binding By similarity
Regioni892 – 895410-formyltetrahydrofolate binding By similarity
Regioni943 – 947510-formyltetrahydrofolate binding By similarity
Regioni973 – 97645'-phosphoribosylglycinamide binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.
In the central section; belongs to the AIR synthase family.
In the C-terminal section; belongs to the GART family.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000030315.
HOVERGENiHBG008333.
KOiK11787.
PhylomeDBiP21872.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
MF_00741_B. AIRS_B.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P21872-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MADRVLVIGS GGREHALAWK LAQSPHVKQV FVAPGNAGTA NSGKISNSAV     50
SVSNHAALAQ FCRDQEIRLV VVGPEVPLAA GIVDDLTAAG VRCFGPTARA 100
AQLESSKSFT KSFLDRHGIP TARWKSFTDP KAACSFINSA NFPALVVKAS 150
GLAAGKGVIV ASNKEEACKA VNDIMQDKTF GTAGETVVVE ELLEGEEVSC 200
LCFTDGVTIA PMPPAQDHKR LKDGDEGPNT GGMGAYSPAP QISKDLLLKI 250
RETVLQKTLD GMRKEGIPYL GVLYAGLMLT KDGPKVLEFN CRFGDPECQV 300
ILPLLKSDLY EVMQAVINKK LSSSMPIWYE DSAAVTVVMA SEGYPGTYPK 350
GLEITGLSKA KELGLEVFHA GTALKDGKVV TNGGRVLTVT AIKEDLMTAL 400
QEANKGVAAI NFKGSIYRKD IGYRAIAFLS QSRGLTYKNS GVDIAAGNIL 450
VQKIKPLAAA TSRSGCNAEL GGFAGLFDLK AAGYKDPILV SGTDGVGTKL 500
KIAQVCKKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFAC GKLDVEVAQG 550
VIAGIAEACQ KAGCALLGGE TAEMPGMYPP GEYDLAGFAV GAVERGQMLP 600
QLERIADGDV VIGVASSGVH SNGYSLVRRI VEKSSLDFSS QVGVSGDQTL 650
GDLLLTPTKI YSKTLLPVLR SGHVKAYAHI TGGGLLENIP RVLPDSFGVV 700
LDALSWKIPE IFCWLHKEGN LSEEEMARTF NCGIGAVLVV QKELAQQVLK 750
DVQKHEAAWL IGKVVPLQKG SAHVKVHNLL QALQANRSLS VHSHIQGKIQ 800
TNKVKVAVLI SGTGTNLEAL INSTKKPTSF AEIVLVVSNK AGVEGLRKAE 850
RAGIPTRVID HKQYGSRTEF DSAVDRVLEE FSVELICLAG FMRILSGPFV 900
KKWEGKILNI HPSLLPSFKG ANAHKLVLEA GVRVTGCTVH FVAEEVDAGA 950
IIFQEAVPVK IGDTVETLSE RVKEAEHRAF PAALQLVASG AVQVGEAGKI 1000
CWK 1003
Length:1,003
Mass (Da):106,544
Last modified:May 1, 1991 - v1
Checksum:iA0C66BA0EBF791DE
GO
Isoform Short (identifier: P21872-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-1003: Missing.

Show »
Length:433
Mass (Da):45,990
Checksum:iC110705BF612798F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei434 – 1003570Missing in isoform Short.
VSP_005516Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56339 mRNA. Translation: CAA39779.1.
X54200 mRNA. Translation: CAA38120.1.
PIRiS12617. AJCHPR.
RefSeqiNP_001001469.1. NM_001001469.1. [P21872-1]
UniGeneiGga.4704.

Genome annotation databases

GeneIDi395315.
KEGGigga:395315.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56339 mRNA. Translation: CAA39779.1 .
X54200 mRNA. Translation: CAA38120.1 .
PIRi S12617. AJCHPR.
RefSeqi NP_001001469.1. NM_001001469.1. [P21872-1 ]
UniGenei Gga.4704.

3D structure databases

ProteinModelPortali P21872.
SMRi P21872. Positions 1-429, 434-789, 803-1002.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000025711.

Proteomic databases

PaxDbi P21872.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 395315.
KEGGi gga:395315.

Organism-specific databases

CTDi 2618.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000030315.
HOVERGENi HBG008333.
KOi K11787.
PhylomeDBi P21872.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
UPA00074 ; UER00126 .
UPA00074 ; UER00129 .
Reactomei REACT_116007. De novo synthesis of IMP.

Miscellaneous databases

NextBioi 20815400.
PROi P21872.

Family and domain databases

Gene3Di 3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPi MF_00138. GARS.
MF_00741_B. AIRS_B.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli."
    Aimi J., Badylak J., Williams J., Chen Z., Zalkin H., Dixon J.E.
    J. Biol. Chem. 265:9011-9014(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli."
    Aimi J., Qiu H., Williams J., Zalkin H., Dixon J.E.
    Nucleic Acids Res. 18:6665-6672(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Tissue: Liver.

Entry informationi

Entry nameiPUR2_CHICK
AccessioniPrimary (citable) accession number: P21872
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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