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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi288 – 2881ManganeseBy similarity
Metal bindingi290 – 2901ManganeseBy similarity
Binding sitei867 – 867110-formyltetrahydrofolateBy similarity
Binding sitei909 – 909110-formyltetrahydrofolateBy similarity
Active sitei911 – 9111Proton donorBy similarity
Sitei947 – 9471Raises pKa of active site HisBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 19963ATPBy similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. methyltransferase activity Source: InterPro
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
  5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC
  6. phosphoribosylglycinamide formyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: Reactome
  2. nucleobase-containing small molecule metabolic process Source: Reactome
  3. purine nucleobase biosynthetic process Source: InterPro
  4. purine nucleobase metabolic process Source: Reactome
  5. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_116007. De novo synthesis of IMP.
UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:GART
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10031003Trifunctional purine biosynthetic protein adenosine-3PRO_0000074936Add
BLAST

Proteomic databases

PaxDbiP21872.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000025711.

Structurei

3D structure databases

ProteinModelPortaliP21872.
SMRiP21872. Positions 1-429, 434-789, 803-1002.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 318208ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni434 – 805372AIRSAdd
BLAST
Regioni806 – 1003198GARTAdd
BLAST
Regioni814 – 81635'-phosphoribosylglycinamide bindingBy similarity
Regioni892 – 895410-formyltetrahydrofolate bindingBy similarity
Regioni943 – 947510-formyltetrahydrofolate bindingBy similarity
Regioni973 – 97645'-phosphoribosylglycinamide bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000030315.
HOVERGENiHBG008333.
InParanoidiP21872.
KOiK11787.
PhylomeDBiP21872.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
MF_00741. AIRS.
MF_01930. PurN.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P21872-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADRVLVIGS GGREHALAWK LAQSPHVKQV FVAPGNAGTA NSGKISNSAV
60 70 80 90 100
SVSNHAALAQ FCRDQEIRLV VVGPEVPLAA GIVDDLTAAG VRCFGPTARA
110 120 130 140 150
AQLESSKSFT KSFLDRHGIP TARWKSFTDP KAACSFINSA NFPALVVKAS
160 170 180 190 200
GLAAGKGVIV ASNKEEACKA VNDIMQDKTF GTAGETVVVE ELLEGEEVSC
210 220 230 240 250
LCFTDGVTIA PMPPAQDHKR LKDGDEGPNT GGMGAYSPAP QISKDLLLKI
260 270 280 290 300
RETVLQKTLD GMRKEGIPYL GVLYAGLMLT KDGPKVLEFN CRFGDPECQV
310 320 330 340 350
ILPLLKSDLY EVMQAVINKK LSSSMPIWYE DSAAVTVVMA SEGYPGTYPK
360 370 380 390 400
GLEITGLSKA KELGLEVFHA GTALKDGKVV TNGGRVLTVT AIKEDLMTAL
410 420 430 440 450
QEANKGVAAI NFKGSIYRKD IGYRAIAFLS QSRGLTYKNS GVDIAAGNIL
460 470 480 490 500
VQKIKPLAAA TSRSGCNAEL GGFAGLFDLK AAGYKDPILV SGTDGVGTKL
510 520 530 540 550
KIAQVCKKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFAC GKLDVEVAQG
560 570 580 590 600
VIAGIAEACQ KAGCALLGGE TAEMPGMYPP GEYDLAGFAV GAVERGQMLP
610 620 630 640 650
QLERIADGDV VIGVASSGVH SNGYSLVRRI VEKSSLDFSS QVGVSGDQTL
660 670 680 690 700
GDLLLTPTKI YSKTLLPVLR SGHVKAYAHI TGGGLLENIP RVLPDSFGVV
710 720 730 740 750
LDALSWKIPE IFCWLHKEGN LSEEEMARTF NCGIGAVLVV QKELAQQVLK
760 770 780 790 800
DVQKHEAAWL IGKVVPLQKG SAHVKVHNLL QALQANRSLS VHSHIQGKIQ
810 820 830 840 850
TNKVKVAVLI SGTGTNLEAL INSTKKPTSF AEIVLVVSNK AGVEGLRKAE
860 870 880 890 900
RAGIPTRVID HKQYGSRTEF DSAVDRVLEE FSVELICLAG FMRILSGPFV
910 920 930 940 950
KKWEGKILNI HPSLLPSFKG ANAHKLVLEA GVRVTGCTVH FVAEEVDAGA
960 970 980 990 1000
IIFQEAVPVK IGDTVETLSE RVKEAEHRAF PAALQLVASG AVQVGEAGKI

CWK
Length:1,003
Mass (Da):106,544
Last modified:April 30, 1991 - v1
Checksum:iA0C66BA0EBF791DE
GO
Isoform Short (identifier: P21872-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-1003: Missing.

Show »
Length:433
Mass (Da):45,990
Checksum:iC110705BF612798F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei434 – 1003570Missing in isoform Short. CuratedVSP_005516Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56339 mRNA. Translation: CAA39779.1.
X54200 mRNA. Translation: CAA38120.1.
PIRiS12617. AJCHPR.
RefSeqiNP_001001469.1. NM_001001469.1. [P21872-1]
UniGeneiGga.4704.

Genome annotation databases

GeneIDi395315.
KEGGigga:395315.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56339 mRNA. Translation: CAA39779.1.
X54200 mRNA. Translation: CAA38120.1.
PIRiS12617. AJCHPR.
RefSeqiNP_001001469.1. NM_001001469.1. [P21872-1]
UniGeneiGga.4704.

3D structure databases

ProteinModelPortaliP21872.
SMRiP21872. Positions 1-429, 434-789, 803-1002.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000025711.

Proteomic databases

PaxDbiP21872.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395315.
KEGGigga:395315.

Organism-specific databases

CTDi2618.

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000030315.
HOVERGENiHBG008333.
InParanoidiP21872.
KOiK11787.
PhylomeDBiP21872.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.
ReactomeiREACT_116007. De novo synthesis of IMP.

Miscellaneous databases

NextBioi20815400.
PROiP21872.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
MF_00741. AIRS.
MF_01930. PurN.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli."
    Aimi J., Badylak J., Williams J., Chen Z., Zalkin H., Dixon J.E.
    J. Biol. Chem. 265:9011-9014(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli."
    Aimi J., Qiu H., Williams J., Zalkin H., Dixon J.E.
    Nucleic Acids Res. 18:6665-6672(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Tissue: Liver.

Entry informationi

Entry nameiPUR2_CHICK
AccessioniPrimary (citable) accession number: P21872
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 1991
Last sequence update: April 30, 1991
Last modified: February 3, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.