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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N1-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Trifunctional purine biosynthetic protein adenosine-3 (GART), Trifunctional purine biosynthetic protein adenosine-3 (LOC427977), Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (GPAT), Amidophosphoribosyltransferase (PPAT), Amidophosphoribosyltransferase (PPAT), Amidophosphoribosyltransferase (PPAT)
  2. Trifunctional purine biosynthetic protein adenosine-3 (GART), Trifunctional purine biosynthetic protein adenosine-3 (LOC427977), Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route).
Proteins known to be involved in this subpathway in this organism are:
  1. Trifunctional purine biosynthetic protein adenosine-3 (GART), Trifunctional purine biosynthetic protein adenosine-3 (LOC427977), Trifunctional purine biosynthetic protein adenosine-3 (GART)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi288ManganeseBy similarity1
Metal bindingi290ManganeseBy similarity1
Binding sitei86710-formyltetrahydrofolateBy similarity1
Binding sitei90910-formyltetrahydrofolateBy similarity1
Active sitei911Proton donorBy similarity1
Sitei947Raises pKa of active site HisBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 199ATPBy similarityAdd BLAST63

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-419140 De novo synthesis of IMP
UniPathwayiUPA00074; UER00125
UPA00074; UER00126
UPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:GART
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749361 – 1003Trifunctional purine biosynthetic protein adenosine-3Add BLAST1003

Proteomic databases

PaxDbiP21872
PRIDEiP21872

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000025711

Structurei

3D structure databases

ProteinModelPortaliP21872
SMRiP21872
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 318ATP-graspAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni434 – 805AIRSAdd BLAST372
Regioni806 – 1003GARTAdd BLAST198
Regioni814 – 8165'-phosphoribosylglycinamide bindingBy similarity3
Regioni892 – 89510-formyltetrahydrofolate bindingBy similarity4
Regioni943 – 94710-formyltetrahydrofolate bindingBy similarity5
Regioni973 – 9765'-phosphoribosylglycinamide bindingBy similarity4

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated

Phylogenomic databases

eggNOGiKOG0237 Eukaryota
KOG3076 Eukaryota
COG0150 LUCA
COG0151 LUCA
COG0299 LUCA
HOGENOMiHOG000030315
HOVERGENiHBG008333
InParanoidiP21872
KOiK11787
PhylomeDBiP21872

Family and domain databases

CDDicd08645 FMT_core_GART, 1 hit
cd02196 PurM, 1 hit
Gene3Di3.30.1330.10, 1 hit
3.30.1490.20, 1 hit
3.90.600.10, 1 hit
3.90.650.10, 1 hit
HAMAPiMF_00741 AIRS, 1 hit
MF_00138 GARS, 1 hit
MF_01930 PurN, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR002376 Formyl_transf_N
IPR036477 Formyl_transf_N_sf
IPR004607 GART
IPR001555 GART_AS
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
IPR011054 Rudment_hybrid_motif
PANTHERiPTHR10520 PTHR10520, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 1 hit
PF02769 AIRS_C, 1 hit
PF00551 Formyl_trans_N, 1 hit
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
SSF53328 SSF53328, 1 hit
SSF55326 SSF55326, 1 hit
SSF56042 SSF56042, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
TIGR00878 purM, 1 hit
TIGR00639 PurN, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit
PS00373 GART, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P21872-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADRVLVIGS GGREHALAWK LAQSPHVKQV FVAPGNAGTA NSGKISNSAV
60 70 80 90 100
SVSNHAALAQ FCRDQEIRLV VVGPEVPLAA GIVDDLTAAG VRCFGPTARA
110 120 130 140 150
AQLESSKSFT KSFLDRHGIP TARWKSFTDP KAACSFINSA NFPALVVKAS
160 170 180 190 200
GLAAGKGVIV ASNKEEACKA VNDIMQDKTF GTAGETVVVE ELLEGEEVSC
210 220 230 240 250
LCFTDGVTIA PMPPAQDHKR LKDGDEGPNT GGMGAYSPAP QISKDLLLKI
260 270 280 290 300
RETVLQKTLD GMRKEGIPYL GVLYAGLMLT KDGPKVLEFN CRFGDPECQV
310 320 330 340 350
ILPLLKSDLY EVMQAVINKK LSSSMPIWYE DSAAVTVVMA SEGYPGTYPK
360 370 380 390 400
GLEITGLSKA KELGLEVFHA GTALKDGKVV TNGGRVLTVT AIKEDLMTAL
410 420 430 440 450
QEANKGVAAI NFKGSIYRKD IGYRAIAFLS QSRGLTYKNS GVDIAAGNIL
460 470 480 490 500
VQKIKPLAAA TSRSGCNAEL GGFAGLFDLK AAGYKDPILV SGTDGVGTKL
510 520 530 540 550
KIAQVCKKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFAC GKLDVEVAQG
560 570 580 590 600
VIAGIAEACQ KAGCALLGGE TAEMPGMYPP GEYDLAGFAV GAVERGQMLP
610 620 630 640 650
QLERIADGDV VIGVASSGVH SNGYSLVRRI VEKSSLDFSS QVGVSGDQTL
660 670 680 690 700
GDLLLTPTKI YSKTLLPVLR SGHVKAYAHI TGGGLLENIP RVLPDSFGVV
710 720 730 740 750
LDALSWKIPE IFCWLHKEGN LSEEEMARTF NCGIGAVLVV QKELAQQVLK
760 770 780 790 800
DVQKHEAAWL IGKVVPLQKG SAHVKVHNLL QALQANRSLS VHSHIQGKIQ
810 820 830 840 850
TNKVKVAVLI SGTGTNLEAL INSTKKPTSF AEIVLVVSNK AGVEGLRKAE
860 870 880 890 900
RAGIPTRVID HKQYGSRTEF DSAVDRVLEE FSVELICLAG FMRILSGPFV
910 920 930 940 950
KKWEGKILNI HPSLLPSFKG ANAHKLVLEA GVRVTGCTVH FVAEEVDAGA
960 970 980 990 1000
IIFQEAVPVK IGDTVETLSE RVKEAEHRAF PAALQLVASG AVQVGEAGKI

CWK
Length:1,003
Mass (Da):106,544
Last modified:May 1, 1991 - v1
Checksum:iA0C66BA0EBF791DE
GO
Isoform Short (identifier: P21872-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-1003: Missing.

Show »
Length:433
Mass (Da):45,990
Checksum:iC110705BF612798F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005516434 – 1003Missing in isoform Short. CuratedAdd BLAST570

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56339 mRNA Translation: CAA39779.1
X54200 mRNA Translation: CAA38120.1
PIRiS12617 AJCHPR
RefSeqiNP_001001469.1, NM_001001469.1 [P21872-1]
UniGeneiGga.4704

Genome annotation databases

GeneIDi395315
KEGGigga:395315

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPUR2_CHICK
AccessioniPrimary (citable) accession number: P21872
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: March 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health