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P21872

- PUR2_CHICK

UniProt

P21872 - PUR2_CHICK

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Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi288 – 2881ManganeseBy similarity
Metal bindingi290 – 2901ManganeseBy similarity
Binding sitei867 – 867110-formyltetrahydrofolateBy similarity
Binding sitei909 – 909110-formyltetrahydrofolateBy similarity
Active sitei911 – 9111Proton donorBy similarity
Sitei947 – 9471Raises pKa of active site HisBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 19963ATPBy similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. methyltransferase activity Source: InterPro
  4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
  5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC
  6. phosphoribosylglycinamide formyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: Reactome
  2. nucleobase-containing small molecule metabolic process Source: Reactome
  3. purine nucleobase biosynthetic process Source: InterPro
  4. purine nucleobase metabolic process Source: Reactome
  5. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_116007. De novo synthesis of IMP.
UniPathwayiUPA00074; UER00125.
UPA00074; UER00126.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Trifunctional purine biosynthetic protein adenosine-3
Including the following 3 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name:
GART
Gene namesi
Name:GART
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10031003Trifunctional purine biosynthetic protein adenosine-3PRO_0000074936Add
BLAST

Proteomic databases

PaxDbiP21872.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000025711.

Structurei

3D structure databases

ProteinModelPortaliP21872.
SMRiP21872. Positions 1-429, 434-789, 803-1002.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 318208ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni434 – 805372AIRSAdd
BLAST
Regioni806 – 1003198GARTAdd
BLAST
Regioni814 – 81635'-phosphoribosylglycinamide bindingBy similarity
Regioni892 – 895410-formyltetrahydrofolate bindingBy similarity
Regioni943 – 947510-formyltetrahydrofolate bindingBy similarity
Regioni973 – 97645'-phosphoribosylglycinamide bindingBy similarity

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the central section; belongs to the AIR synthase family.Curated
In the C-terminal section; belongs to the GART family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000030315.
HOVERGENiHBG008333.
InParanoidiP21872.
KOiK11787.
PhylomeDBiP21872.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
MF_00741. AIRS.
MF_01930. PurN.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P21872-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADRVLVIGS GGREHALAWK LAQSPHVKQV FVAPGNAGTA NSGKISNSAV
60 70 80 90 100
SVSNHAALAQ FCRDQEIRLV VVGPEVPLAA GIVDDLTAAG VRCFGPTARA
110 120 130 140 150
AQLESSKSFT KSFLDRHGIP TARWKSFTDP KAACSFINSA NFPALVVKAS
160 170 180 190 200
GLAAGKGVIV ASNKEEACKA VNDIMQDKTF GTAGETVVVE ELLEGEEVSC
210 220 230 240 250
LCFTDGVTIA PMPPAQDHKR LKDGDEGPNT GGMGAYSPAP QISKDLLLKI
260 270 280 290 300
RETVLQKTLD GMRKEGIPYL GVLYAGLMLT KDGPKVLEFN CRFGDPECQV
310 320 330 340 350
ILPLLKSDLY EVMQAVINKK LSSSMPIWYE DSAAVTVVMA SEGYPGTYPK
360 370 380 390 400
GLEITGLSKA KELGLEVFHA GTALKDGKVV TNGGRVLTVT AIKEDLMTAL
410 420 430 440 450
QEANKGVAAI NFKGSIYRKD IGYRAIAFLS QSRGLTYKNS GVDIAAGNIL
460 470 480 490 500
VQKIKPLAAA TSRSGCNAEL GGFAGLFDLK AAGYKDPILV SGTDGVGTKL
510 520 530 540 550
KIAQVCKKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFAC GKLDVEVAQG
560 570 580 590 600
VIAGIAEACQ KAGCALLGGE TAEMPGMYPP GEYDLAGFAV GAVERGQMLP
610 620 630 640 650
QLERIADGDV VIGVASSGVH SNGYSLVRRI VEKSSLDFSS QVGVSGDQTL
660 670 680 690 700
GDLLLTPTKI YSKTLLPVLR SGHVKAYAHI TGGGLLENIP RVLPDSFGVV
710 720 730 740 750
LDALSWKIPE IFCWLHKEGN LSEEEMARTF NCGIGAVLVV QKELAQQVLK
760 770 780 790 800
DVQKHEAAWL IGKVVPLQKG SAHVKVHNLL QALQANRSLS VHSHIQGKIQ
810 820 830 840 850
TNKVKVAVLI SGTGTNLEAL INSTKKPTSF AEIVLVVSNK AGVEGLRKAE
860 870 880 890 900
RAGIPTRVID HKQYGSRTEF DSAVDRVLEE FSVELICLAG FMRILSGPFV
910 920 930 940 950
KKWEGKILNI HPSLLPSFKG ANAHKLVLEA GVRVTGCTVH FVAEEVDAGA
960 970 980 990 1000
IIFQEAVPVK IGDTVETLSE RVKEAEHRAF PAALQLVASG AVQVGEAGKI

CWK
Length:1,003
Mass (Da):106,544
Last modified:May 1, 1991 - v1
Checksum:iA0C66BA0EBF791DE
GO
Isoform Short (identifier: P21872-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-1003: Missing.

Show »
Length:433
Mass (Da):45,990
Checksum:iC110705BF612798F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei434 – 1003570Missing in isoform Short. CuratedVSP_005516Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56339 mRNA. Translation: CAA39779.1.
X54200 mRNA. Translation: CAA38120.1.
PIRiS12617. AJCHPR.
RefSeqiNP_001001469.1. NM_001001469.1. [P21872-1]
UniGeneiGga.4704.

Genome annotation databases

GeneIDi395315.
KEGGigga:395315.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56339 mRNA. Translation: CAA39779.1 .
X54200 mRNA. Translation: CAA38120.1 .
PIRi S12617. AJCHPR.
RefSeqi NP_001001469.1. NM_001001469.1. [P21872-1 ]
UniGenei Gga.4704.

3D structure databases

ProteinModelPortali P21872.
SMRi P21872. Positions 1-429, 434-789, 803-1002.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000025711.

Proteomic databases

PaxDbi P21872.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 395315.
KEGGi gga:395315.

Organism-specific databases

CTDi 2618.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000030315.
HOVERGENi HBG008333.
InParanoidi P21872.
KOi K11787.
PhylomeDBi P21872.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
UPA00074 ; UER00126 .
UPA00074 ; UER00129 .
Reactomei REACT_116007. De novo synthesis of IMP.

Miscellaneous databases

NextBioi 20815400.
PROi P21872.

Family and domain databases

Gene3Di 3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.170. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPi MF_00138. GARS.
MF_00741. AIRS.
MF_01930. PurN.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR002376. Formyl_transf_N.
IPR001555. GART_AS.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR004607. PurN_trans.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF53328. SSF53328. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
TIGR00639. PurN. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
PS00373. GART. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli."
    Aimi J., Badylak J., Williams J., Chen Z., Zalkin H., Dixon J.E.
    J. Biol. Chem. 265:9011-9014(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli."
    Aimi J., Qiu H., Williams J., Zalkin H., Dixon J.E.
    Nucleic Acids Res. 18:6665-6672(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    Tissue: Liver.

Entry informationi

Entry nameiPUR2_CHICK
AccessioniPrimary (citable) accession number: P21872
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3