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P21872

- PUR2_CHICK

UniProt

P21872 - PUR2_CHICK

Protein

Trifunctional purine biosynthetic protein adenosine-3

Gene

GART

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
    ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
    10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi288 – 2881ManganeseBy similarity
    Metal bindingi290 – 2901ManganeseBy similarity
    Binding sitei867 – 867110-formyltetrahydrofolateBy similarity
    Binding sitei909 – 909110-formyltetrahydrofolateBy similarity
    Active sitei911 – 9111Proton donorBy similarity
    Sitei947 – 9471Raises pKa of active site HisBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi137 – 19963ATPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. methyltransferase activity Source: InterPro
    4. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC
    5. phosphoribosylformylglycinamidine cyclo-ligase activity Source: UniProtKB-EC
    6. phosphoribosylglycinamide formyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: Reactome
    2. nucleobase-containing small molecule metabolic process Source: Reactome
    3. purine nucleobase biosynthetic process Source: InterPro
    4. purine nucleobase metabolic process Source: Reactome
    5. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Ligase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_116007. De novo synthesis of IMP.
    UniPathwayiUPA00074; UER00125.
    UPA00074; UER00126.
    UPA00074; UER00129.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trifunctional purine biosynthetic protein adenosine-3
    Including the following 3 domains:
    Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name:
    GARS
    Phosphoribosylglycinamide synthetase
    Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
    Alternative name(s):
    AIR synthase
    Short name:
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    Phosphoribosylglycinamide formyltransferase (EC:2.1.2.2)
    Alternative name(s):
    5'-phosphoribosylglycinamide transformylase
    GAR transformylase
    Short name:
    GART
    Gene namesi
    Name:GART
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10031003Trifunctional purine biosynthetic protein adenosine-3PRO_0000074936Add
    BLAST

    Proteomic databases

    PaxDbiP21872.

    Interactioni

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000025711.

    Structurei

    3D structure databases

    ProteinModelPortaliP21872.
    SMRiP21872. Positions 1-429, 434-789, 803-1002.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini111 – 318208ATP-graspAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni434 – 805372AIRSAdd
    BLAST
    Regioni806 – 1003198GARTAdd
    BLAST
    Regioni814 – 81635'-phosphoribosylglycinamide bindingBy similarity
    Regioni892 – 895410-formyltetrahydrofolate bindingBy similarity
    Regioni943 – 947510-formyltetrahydrofolate bindingBy similarity
    Regioni973 – 97645'-phosphoribosylglycinamide bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the GARS family.Curated
    In the central section; belongs to the AIR synthase family.Curated
    In the C-terminal section; belongs to the GART family.Curated
    Contains 1 ATP-grasp domain.Curated

    Phylogenomic databases

    eggNOGiCOG0151.
    HOGENOMiHOG000030315.
    HOVERGENiHBG008333.
    KOiK11787.
    PhylomeDBiP21872.

    Family and domain databases

    Gene3Di3.30.1330.10. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.170. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 1 hit.
    HAMAPiMF_00138. GARS.
    MF_00741_B. AIRS_B.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR002376. Formyl_transf_N.
    IPR001555. GART_AS.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR004607. PurN_trans.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    PF00551. Formyl_trans_N. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF53328. SSF53328. 1 hit.
    SSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsiTIGR00877. purD. 1 hit.
    TIGR00878. purM. 1 hit.
    TIGR00639. PurN. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    PS00373. GART. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P21872-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADRVLVIGS GGREHALAWK LAQSPHVKQV FVAPGNAGTA NSGKISNSAV     50
    SVSNHAALAQ FCRDQEIRLV VVGPEVPLAA GIVDDLTAAG VRCFGPTARA 100
    AQLESSKSFT KSFLDRHGIP TARWKSFTDP KAACSFINSA NFPALVVKAS 150
    GLAAGKGVIV ASNKEEACKA VNDIMQDKTF GTAGETVVVE ELLEGEEVSC 200
    LCFTDGVTIA PMPPAQDHKR LKDGDEGPNT GGMGAYSPAP QISKDLLLKI 250
    RETVLQKTLD GMRKEGIPYL GVLYAGLMLT KDGPKVLEFN CRFGDPECQV 300
    ILPLLKSDLY EVMQAVINKK LSSSMPIWYE DSAAVTVVMA SEGYPGTYPK 350
    GLEITGLSKA KELGLEVFHA GTALKDGKVV TNGGRVLTVT AIKEDLMTAL 400
    QEANKGVAAI NFKGSIYRKD IGYRAIAFLS QSRGLTYKNS GVDIAAGNIL 450
    VQKIKPLAAA TSRSGCNAEL GGFAGLFDLK AAGYKDPILV SGTDGVGTKL 500
    KIAQVCKKHD TIGQDLVAMC VNDILAQGAE PLFFLDYFAC GKLDVEVAQG 550
    VIAGIAEACQ KAGCALLGGE TAEMPGMYPP GEYDLAGFAV GAVERGQMLP 600
    QLERIADGDV VIGVASSGVH SNGYSLVRRI VEKSSLDFSS QVGVSGDQTL 650
    GDLLLTPTKI YSKTLLPVLR SGHVKAYAHI TGGGLLENIP RVLPDSFGVV 700
    LDALSWKIPE IFCWLHKEGN LSEEEMARTF NCGIGAVLVV QKELAQQVLK 750
    DVQKHEAAWL IGKVVPLQKG SAHVKVHNLL QALQANRSLS VHSHIQGKIQ 800
    TNKVKVAVLI SGTGTNLEAL INSTKKPTSF AEIVLVVSNK AGVEGLRKAE 850
    RAGIPTRVID HKQYGSRTEF DSAVDRVLEE FSVELICLAG FMRILSGPFV 900
    KKWEGKILNI HPSLLPSFKG ANAHKLVLEA GVRVTGCTVH FVAEEVDAGA 950
    IIFQEAVPVK IGDTVETLSE RVKEAEHRAF PAALQLVASG AVQVGEAGKI 1000
    CWK 1003
    Length:1,003
    Mass (Da):106,544
    Last modified:May 1, 1991 - v1
    Checksum:iA0C66BA0EBF791DE
    GO
    Isoform Short (identifier: P21872-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         434-1003: Missing.

    Show »
    Length:433
    Mass (Da):45,990
    Checksum:iC110705BF612798F
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei434 – 1003570Missing in isoform Short. CuratedVSP_005516Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56339 mRNA. Translation: CAA39779.1.
    X54200 mRNA. Translation: CAA38120.1.
    PIRiS12617. AJCHPR.
    RefSeqiNP_001001469.1. NM_001001469.1. [P21872-1]
    UniGeneiGga.4704.

    Genome annotation databases

    GeneIDi395315.
    KEGGigga:395315.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56339 mRNA. Translation: CAA39779.1 .
    X54200 mRNA. Translation: CAA38120.1 .
    PIRi S12617. AJCHPR.
    RefSeqi NP_001001469.1. NM_001001469.1. [P21872-1 ]
    UniGenei Gga.4704.

    3D structure databases

    ProteinModelPortali P21872.
    SMRi P21872. Positions 1-429, 434-789, 803-1002.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000025711.

    Proteomic databases

    PaxDbi P21872.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 395315.
    KEGGi gga:395315.

    Organism-specific databases

    CTDi 2618.

    Phylogenomic databases

    eggNOGi COG0151.
    HOGENOMi HOG000030315.
    HOVERGENi HBG008333.
    KOi K11787.
    PhylomeDBi P21872.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00125 .
    UPA00074 ; UER00126 .
    UPA00074 ; UER00129 .
    Reactomei REACT_116007. De novo synthesis of IMP.

    Miscellaneous databases

    NextBioi 20815400.
    PROi P21872.

    Family and domain databases

    Gene3Di 3.30.1330.10. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.170. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 1 hit.
    HAMAPi MF_00138. GARS.
    MF_00741_B. AIRS_B.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR002376. Formyl_transf_N.
    IPR001555. GART_AS.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR004607. PurN_trans.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    PF00551. Formyl_trans_N. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF53328. SSF53328. 1 hit.
    SSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsi TIGR00877. purD. 1 hit.
    TIGR00878. purM. 1 hit.
    TIGR00639. PurN. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    PS00373. GART. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli."
      Aimi J., Badylak J., Williams J., Chen Z., Zalkin H., Dixon J.E.
      J. Biol. Chem. 265:9011-9014(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli."
      Aimi J., Qiu H., Williams J., Zalkin H., Dixon J.E.
      Nucleic Acids Res. 18:6665-6672(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
      Tissue: Liver.

    Entry informationi

    Entry nameiPUR2_CHICK
    AccessioniPrimary (citable) accession number: P21872
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3