ID   KDPE_ECOLI              Reviewed;         225 AA.
AC   P21866; P75739; P76822;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 3.
DT   27-MAR-2024, entry version 184.
DE   RecName: Full=KDP operon transcriptional regulatory protein KdpE;
GN   Name=kdpE; OrderedLocusNames=b0694, JW5096;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=K12;
RX   PubMed=1532388; DOI=10.1128/jb.174.7.2152-2159.1992;
RA   Walderhaug M.O., Polarek J.W., Voelkner P., Daniel J.M., Hesse J.E.,
RA   Altendorf K., Epstein W.;
RT   "KdpD and KdpE, proteins that control expression of the kdpABC operon, are
RT   members of the two-component sensor-effector class of regulators.";
RL   J. Bacteriol. 174:2152-2159(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   IDENTIFICATION BY 2D-GEL.
RX   PubMed=9298644; DOI=10.1002/elps.1150180805;
RA   VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT   "Escherichia coli proteome analysis using the gene-protein database.";
RL   Electrophoresis 18:1243-1251(1997).
CC   -!- FUNCTION: Member of the two-component regulatory system KdpD/KdpE
CC       involved in the regulation of the kdp operon.
CC       {ECO:0000269|PubMed:1532388}.
CC   -!- INTERACTION:
CC       P21866; P21865: kdpD; NbExp=5; IntAct=EBI-6403634, EBI-1123100;
CC       P21866; P52108: rstA; NbExp=2; IntAct=EBI-6403634, EBI-558990;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:1532388}.
CC   -!- PTM: Phosphorylated by KdpD. {ECO:0000305}.
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DR   EMBL; M36066; AAA24042.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73788.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35351.2; -; Genomic_DNA.
DR   PIR; E64804; E64804.
DR   RefSeq; NP_415222.1; NC_000913.3.
DR   RefSeq; WP_000186076.1; NZ_SSZK01000045.1.
DR   PDB; 1ZH2; X-ray; 2.00 A; A/B=1-121.
DR   PDB; 1ZH4; X-ray; 2.20 A; A/B=1-121.
DR   PDB; 3ZQ7; X-ray; 2.52 A; A=124-225.
DR   PDB; 4KFC; X-ray; 2.53 A; A/B=3-225.
DR   PDB; 4KNY; X-ray; 2.94 A; A/B=3-225.
DR   PDB; 4L85; X-ray; 2.20 A; A/B/C=3-121.
DR   PDB; 6LGQ; X-ray; 3.00 A; D=1-225.
DR   PDBsum; 1ZH2; -.
DR   PDBsum; 1ZH4; -.
DR   PDBsum; 3ZQ7; -.
DR   PDBsum; 4KFC; -.
DR   PDBsum; 4KNY; -.
DR   PDBsum; 4L85; -.
DR   PDBsum; 6LGQ; -.
DR   AlphaFoldDB; P21866; -.
DR   SMR; P21866; -.
DR   BioGRID; 4259924; 85.
DR   BioGRID; 849679; 1.
DR   DIP; DIP-10063N; -.
DR   IntAct; P21866; 2.
DR   STRING; 511145.b0694; -.
DR   jPOST; P21866; -.
DR   PaxDb; 511145-b0694; -.
DR   EnsemblBacteria; AAC73788; AAC73788; b0694.
DR   GeneID; 945302; -.
DR   KEGG; ecj:JW5096; -.
DR   KEGG; eco:b0694; -.
DR   PATRIC; fig|1411691.4.peg.1581; -.
DR   EchoBASE; EB0512; -.
DR   eggNOG; COG0745; Bacteria.
DR   HOGENOM; CLU_000445_30_8_6; -.
DR   InParanoid; P21866; -.
DR   OMA; KARKYEV; -.
DR   OrthoDB; 9802426at2; -.
DR   PhylomeDB; P21866; -.
DR   BioCyc; EcoCyc:KDPE-MONOMER; -.
DR   EvolutionaryTrace; P21866; -.
DR   PRO; PR:P21866; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR   GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:EcoCyc.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IDA:EcoCyc.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:EcoCyc.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IMP:EcoCyc.
DR   CDD; cd17620; REC_OmpR_KdpE-like; 1.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 3.40.50.2300; -; 1.
DR   Gene3D; 6.10.250.690; -; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111:SF50; KDP OPERON TRANSCRIPTIONAL REGULATORY PROTEIN KDPE; 1.
DR   PANTHER; PTHR48111; REGULATOR OF RPOS; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; CheY-like; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..225
FT                   /note="KDP operon transcriptional regulatory protein KdpE"
FT                   /id="PRO_0000081113"
FT   DOMAIN          3..116
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        126..225
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         52
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   CONFLICT        160..176
FT                   /note="LAVLLNNAGKVLTQRQL -> AGRCSTMPEKYSPSGPV (in Ref. 1;
FT                   AAA24042)"
FT                   /evidence="ECO:0000305"
FT   STRAND          3..7
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   HELIX           11..22
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   TURN            23..25
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   STRAND          27..33
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   HELIX           34..44
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   STRAND          47..55
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   STRAND          56..58
FT                   /evidence="ECO:0007829|PDB:4KNY"
FT   HELIX           60..68
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   STRAND          75..80
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   HELIX           84..93
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   STRAND          96..102
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   HELIX           105..119
FT                   /evidence="ECO:0007829|PDB:1ZH2"
FT   STRAND          127..130
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   STRAND          135..137
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   TURN            138..141
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   STRAND          142..145
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   STRAND          148..150
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   HELIX           154..165
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   TURN            166..168
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   STRAND          170..172
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   HELIX           173..180
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   STRAND          182..184
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   HELIX           189..203
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   STRAND          211..216
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   TURN            217..219
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
FT   STRAND          220..223
FT                   /evidence="ECO:0007829|PDB:3ZQ7"
SQ   SEQUENCE   225 AA;  25362 MW;  6C2D029F82706F6D CRC64;
     MTNVLIVEDE QAIRRFLRTA LEGDGMRVFE AETLQRGLLE AATRKPDLII LDLGLPDGDG
     IEFIRDLRQW SAVPVIVLSA RSEESDKIAA LDAGADDYLS KPFGIGELQA RLRVALRRHS
     ATTAPDPLVK FSDVTVDLAA RVIHRGEEEV HLTPIEFRLL AVLLNNAGKV LTQRQLLNQV
     WGPNAVEHSH YLRIYMGHLR QKLEQDPARP RHFITETGIG YRFML
//