Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

KDP operon transcriptional regulatory protein KdpE

Gene

kdpE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon.1 Publication

GO - Molecular functioni

  1. bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding Source: EcoCyc
  2. bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding transcription factor activity Source: EcoCyc

GO - Biological processi

  1. phosphorelay signal transduction system Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: EcoCyc
  3. transcription from bacterial-type RNA polymerase promoter Source: GOC
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:KDPE-MONOMER.
ECOL316407:JW5096-MONOMER.
RETL1328306-WGS:GSTH-6121-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
KDP operon transcriptional regulatory protein KdpE
Gene namesi
Name:kdpE
Ordered Locus Names:b0694, JW5096
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10517. kdpE.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225KDP operon transcriptional regulatory protein KdpEPRO_0000081113Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 5214-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by KdpD.Curated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21866.

Expressioni

Gene expression databases

GenevestigatoriP21866.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
kdpDP218655EBI-6403634,EBI-1123100
rstAP521082EBI-6403634,EBI-558990

Protein-protein interaction databases

DIPiDIP-10063N.
IntActiP21866. 2 interactions.
STRINGi511145.b0694.

Structurei

Secondary structure

1
225
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75Combined sources
Helixi11 – 2212Combined sources
Turni23 – 253Combined sources
Beta strandi27 – 337Combined sources
Helixi34 – 4411Combined sources
Beta strandi47 – 559Combined sources
Beta strandi56 – 583Combined sources
Helixi60 – 689Combined sources
Beta strandi75 – 806Combined sources
Helixi84 – 9310Combined sources
Beta strandi96 – 1027Combined sources
Helixi105 – 11915Combined sources
Beta strandi127 – 1304Combined sources
Beta strandi135 – 1373Combined sources
Turni138 – 1414Combined sources
Beta strandi142 – 1454Combined sources
Beta strandi148 – 1503Combined sources
Helixi154 – 16512Combined sources
Turni166 – 1683Combined sources
Beta strandi170 – 1723Combined sources
Helixi173 – 1808Combined sources
Beta strandi182 – 1843Combined sources
Helixi189 – 20315Combined sources
Beta strandi211 – 2166Combined sources
Turni217 – 2193Combined sources
Beta strandi220 – 2234Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZH2X-ray2.00A/B1-121[»]
1ZH4X-ray2.20A/B1-121[»]
3ZQ7X-ray2.52A124-225[»]
4KFCX-ray2.53A/B3-225[»]
4KNYX-ray2.94A/B3-225[»]
4L85X-ray2.20A/B/C3-121[»]
ProteinModelPortaliP21866.
SMRiP21866. Positions 1-225.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21866.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 116116Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034815.
InParanoidiP21866.
KOiK07667.
OMAiLEGEGCR.
OrthoDBiEOG6G4VQG.
PhylomeDBiP21866.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21866-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNVLIVEDE QAIRRFLRTA LEGDGMRVFE AETLQRGLLE AATRKPDLII
60 70 80 90 100
LDLGLPDGDG IEFIRDLRQW SAVPVIVLSA RSEESDKIAA LDAGADDYLS
110 120 130 140 150
KPFGIGELQA RLRVALRRHS ATTAPDPLVK FSDVTVDLAA RVIHRGEEEV
160 170 180 190 200
HLTPIEFRLL AVLLNNAGKV LTQRQLLNQV WGPNAVEHSH YLRIYMGHLR
210 220
QKLEQDPARP RHFITETGIG YRFML
Length:225
Mass (Da):25,362
Last modified:November 1, 1997 - v3
Checksum:i6C2D029F82706F6D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 17617LAVLL…TQRQL → AGRCSTMPEKYSPSGPV in AAA24042 (PubMed:1532388).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36066 Genomic DNA. Translation: AAA24042.1.
U00096 Genomic DNA. Translation: AAC73788.1.
AP009048 Genomic DNA. Translation: BAA35351.2.
PIRiE64804.
RefSeqiNP_415222.1. NC_000913.3.
YP_488974.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73788; AAC73788; b0694.
BAA35351; BAA35351; BAA35351.
GeneIDi12930930.
945302.
KEGGiecj:Y75_p0673.
eco:b0694.
PATRICi32116583. VBIEscCol129921_0724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36066 Genomic DNA. Translation: AAA24042.1.
U00096 Genomic DNA. Translation: AAC73788.1.
AP009048 Genomic DNA. Translation: BAA35351.2.
PIRiE64804.
RefSeqiNP_415222.1. NC_000913.3.
YP_488974.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZH2X-ray2.00A/B1-121[»]
1ZH4X-ray2.20A/B1-121[»]
3ZQ7X-ray2.52A124-225[»]
4KFCX-ray2.53A/B3-225[»]
4KNYX-ray2.94A/B3-225[»]
4L85X-ray2.20A/B/C3-121[»]
ProteinModelPortaliP21866.
SMRiP21866. Positions 1-225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10063N.
IntActiP21866. 2 interactions.
STRINGi511145.b0694.

Proteomic databases

PaxDbiP21866.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73788; AAC73788; b0694.
BAA35351; BAA35351; BAA35351.
GeneIDi12930930.
945302.
KEGGiecj:Y75_p0673.
eco:b0694.
PATRICi32116583. VBIEscCol129921_0724.

Organism-specific databases

EchoBASEiEB0512.
EcoGeneiEG10517. kdpE.

Phylogenomic databases

eggNOGiCOG0745.
HOGENOMiHOG000034815.
InParanoidiP21866.
KOiK07667.
OMAiLEGEGCR.
OrthoDBiEOG6G4VQG.
PhylomeDBiP21866.

Enzyme and pathway databases

BioCyciEcoCyc:KDPE-MONOMER.
ECOL316407:JW5096-MONOMER.
RETL1328306-WGS:GSTH-6121-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP21866.
PROiP21866.

Gene expression databases

GenevestigatoriP21866.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF52172. SSF52172. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators."
    Walderhaug M.O., Polarek J.W., Voelkner P., Daniel J.M., Hesse J.E., Altendorf K., Epstein W.
    J. Bacteriol. 174:2152-2159(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiKDPE_ECOLI
AccessioniPrimary (citable) accession number: P21866
Secondary accession number(s): P75739, P76822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1997
Last modified: January 7, 2015
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.