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P21866

- KDPE_ECOLI

UniProt

P21866 - KDPE_ECOLI

Protein

KDP operon transcriptional regulatory protein KdpE

Gene

kdpE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 3 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon.1 Publication

    GO - Molecular functioni

    1. bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding Source: EcoCyc
    2. bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding transcription factor activity Source: EcoCyc
    3. phosphorelay response regulator activity Source: InterPro
    4. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: EcoCyc
    2. transcription from bacterial-type RNA polymerase promoter Source: GOC

    Keywords - Biological processi

    Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:KDPE-MONOMER.
    ECOL316407:JW5096-MONOMER.
    RETL1328306-WGS:GSTH-6121-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    KDP operon transcriptional regulatory protein KdpE
    Gene namesi
    Name:kdpE
    Ordered Locus Names:b0694, JW5096
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10517. kdpE.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 225225KDP operon transcriptional regulatory protein KdpEPRO_0000081113Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei52 – 5214-aspartylphosphatePROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylated by KdpD.Curated

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP21866.

    Expressioni

    Gene expression databases

    GenevestigatoriP21866.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    kdpDP218655EBI-6403634,EBI-1123100
    rstAP521082EBI-6403634,EBI-558990

    Protein-protein interaction databases

    DIPiDIP-10063N.
    IntActiP21866. 2 interactions.
    STRINGi511145.b0694.

    Structurei

    Secondary structure

    1
    225
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 75
    Helixi11 – 2212
    Turni23 – 253
    Beta strandi27 – 337
    Helixi34 – 4411
    Beta strandi47 – 559
    Beta strandi56 – 583
    Helixi60 – 689
    Beta strandi75 – 806
    Helixi84 – 9310
    Beta strandi96 – 1027
    Helixi105 – 11915
    Beta strandi127 – 1304
    Beta strandi135 – 1373
    Turni138 – 1414
    Beta strandi142 – 1454
    Beta strandi148 – 1503
    Helixi154 – 16512
    Turni166 – 1683
    Beta strandi170 – 1723
    Helixi173 – 1808
    Beta strandi182 – 1843
    Helixi189 – 20315
    Beta strandi211 – 2166
    Turni217 – 2193
    Beta strandi220 – 2234

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZH2X-ray2.00A/B1-121[»]
    1ZH4X-ray2.20A/B1-121[»]
    3ZQ7X-ray2.52A124-225[»]
    4KFCX-ray2.53A/B3-225[»]
    4KNYX-ray2.94A/B3-225[»]
    4L85X-ray2.20A/B/C3-121[»]
    ProteinModelPortaliP21866.
    SMRiP21866. Positions 1-225.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21866.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 116116Response regulatoryPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0745.
    HOGENOMiHOG000034815.
    KOiK07667.
    OMAiQPNLIQT.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiP21866.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view]
    SMARTiSM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52172. SSF52172. 1 hit.
    PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21866-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNVLIVEDE QAIRRFLRTA LEGDGMRVFE AETLQRGLLE AATRKPDLII    50
    LDLGLPDGDG IEFIRDLRQW SAVPVIVLSA RSEESDKIAA LDAGADDYLS 100
    KPFGIGELQA RLRVALRRHS ATTAPDPLVK FSDVTVDLAA RVIHRGEEEV 150
    HLTPIEFRLL AVLLNNAGKV LTQRQLLNQV WGPNAVEHSH YLRIYMGHLR 200
    QKLEQDPARP RHFITETGIG YRFML 225
    Length:225
    Mass (Da):25,362
    Last modified:November 1, 1997 - v3
    Checksum:i6C2D029F82706F6D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti160 – 17617LAVLL…TQRQL → AGRCSTMPEKYSPSGPV in AAA24042. (PubMed:1532388)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36066 Genomic DNA. Translation: AAA24042.1.
    U00096 Genomic DNA. Translation: AAC73788.1.
    AP009048 Genomic DNA. Translation: BAA35351.2.
    PIRiE64804.
    RefSeqiNP_415222.1. NC_000913.3.
    YP_488974.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73788; AAC73788; b0694.
    BAA35351; BAA35351; BAA35351.
    GeneIDi12930930.
    945302.
    KEGGiecj:Y75_p0673.
    eco:b0694.
    PATRICi32116583. VBIEscCol129921_0724.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36066 Genomic DNA. Translation: AAA24042.1 .
    U00096 Genomic DNA. Translation: AAC73788.1 .
    AP009048 Genomic DNA. Translation: BAA35351.2 .
    PIRi E64804.
    RefSeqi NP_415222.1. NC_000913.3.
    YP_488974.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZH2 X-ray 2.00 A/B 1-121 [» ]
    1ZH4 X-ray 2.20 A/B 1-121 [» ]
    3ZQ7 X-ray 2.52 A 124-225 [» ]
    4KFC X-ray 2.53 A/B 3-225 [» ]
    4KNY X-ray 2.94 A/B 3-225 [» ]
    4L85 X-ray 2.20 A/B/C 3-121 [» ]
    ProteinModelPortali P21866.
    SMRi P21866. Positions 1-225.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10063N.
    IntActi P21866. 2 interactions.
    STRINGi 511145.b0694.

    Proteomic databases

    PaxDbi P21866.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73788 ; AAC73788 ; b0694 .
    BAA35351 ; BAA35351 ; BAA35351 .
    GeneIDi 12930930.
    945302.
    KEGGi ecj:Y75_p0673.
    eco:b0694.
    PATRICi 32116583. VBIEscCol129921_0724.

    Organism-specific databases

    EchoBASEi EB0512.
    EcoGenei EG10517. kdpE.

    Phylogenomic databases

    eggNOGi COG0745.
    HOGENOMi HOG000034815.
    KOi K07667.
    OMAi QPNLIQT.
    OrthoDBi EOG6G4VQG.
    PhylomeDBi P21866.

    Enzyme and pathway databases

    BioCyci EcoCyc:KDPE-MONOMER.
    ECOL316407:JW5096-MONOMER.
    RETL1328306-WGS:GSTH-6121-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P21866.
    PROi P21866.

    Gene expression databases

    Genevestigatori P21866.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00072. Response_reg. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view ]
    SMARTi SM00448. REC. 1 hit.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52172. SSF52172. 1 hit.
    PROSITEi PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators."
      Walderhaug M.O., Polarek J.W., Voelkner P., Daniel J.M., Hesse J.E., Altendorf K., Epstein W.
      J. Bacteriol. 174:2152-2159(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.

    Entry informationi

    Entry nameiKDPE_ECOLI
    AccessioniPrimary (citable) accession number: P21866
    Secondary accession number(s): P75739, P76822
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 129 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3