KDP operon transcriptional regulatory protein KdpE

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P21866 (KDPE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
KDP operon transcriptional regulatory protein KdpE

Gene names

Name:	kdpE
Ordered Locus Names:	b0694, JW5096

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon. Ref.1
Subcellular location	Cytoplasm Ref.1.
Post-translational modification	Phosphorylated by KdpD Probable.
Sequence similarities	Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding Inferred from direct assay PubMed 1630316. Source: EcoCyc bacterial-type RNA polymerase core promoter proximal region sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 1630316. Source: EcoCyc phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
kdpD	P21865	4	EBI-6403634,EBI-1123100
rstA	P52108	2	EBI-6403634,EBI-558990

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 225

225

KDP operon transcriptional regulatory protein KdpE

PRO_0000081113

Regions

Domain

1 – 116

116

Response regulatory

Amino acid modifications

Modified residue

4-aspartylphosphate By similarity

Experimental info

Sequence conflict

160 – 176

LAVLL…TQRQL → AGRCSTMPEKYSPSGPV in AAA24042. Ref.1

Secondary structure

225

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P21866 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 6C2D029F82706F6D
Show »

FASTA

225

25,362

        10         20         30         40         50         60 
MTNVLIVEDE QAIRRFLRTA LEGDGMRVFE AETLQRGLLE AATRKPDLII LDLGLPDGDG 

        70         80         90        100        110        120 
IEFIRDLRQW SAVPVIVLSA RSEESDKIAA LDAGADDYLS KPFGIGELQA RLRVALRRHS 

       130        140        150        160        170        180 
ATTAPDPLVK FSDVTVDLAA RVIHRGEEEV HLTPIEFRLL AVLLNNAGKV LTQRQLLNQV 

       190        200        210        220 
WGPNAVEHSH YLRIYMGHLR QKLEQDPARP RHFITETGIG YRFML

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators." Walderhaug M.O., Polarek J.W., Voelkner P., Daniel J.M., Hesse J.E., Altendorf K., Epstein W. J. Bacteriol. 174:2152-2159(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M36066 Genomic DNA. Translation: AAA24042.1.
U00096 Genomic DNA. Translation: AAC73788.1.
AP009048 Genomic DNA. Translation: BAA35351.2.

PIR

E64804.

RefSeq

NP_415222.1. NC_000913.2.
YP_488974.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZH2	X-ray	2.00	A/B	1-120	[»]
1ZH4	X-ray	2.20	A/B	1-120	[»]
3ZQ7	X-ray	2.52	A	124-225	[»]

ProteinModelPortal

P21866.

SMR

P21866. Positions 1-120, 125-225.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10063N.

IntAct

P21866. 2 interactions.

STRING

511145.b0694.

Proteomic databases

PaxDb

P21866.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73788; AAC73788; b0694.
BAA35351; BAA35351; BAA35351.

GeneID

12930930.
945302.

KEGG

ecj:Y75_p0673.
eco:b0694.

PATRIC

32116583. VBIEscCol129921_0724.

Organism-specific databases

EchoBASE

EB0512.

EcoGene

EG10517. kdpE.

Phylogenomic databases

eggNOG

COG0745.

HOGENOM

HOG000034815.

K07667.

OMA

QWSQMPI.

ProtClustDB

PRK10529.

Enzyme and pathway databases

BioCyc

EcoCyc:KDPE-MONOMER.
ECOL316407:JW5096-MONOMER.

Gene expression databases

Genevestigator

P21866.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR011006. CheY-like_superfamily.
IPR001867. Sig_transdc_resp-reg_C.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00072. Response_reg. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]

SMART

SM00448. REC. 1 hit.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]

SUPFAM

SSF52172. CheY_like. 1 hit.

PROSITE

PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P21866.

Entry information

Entry name

KDPE_ECOLI

Accession

Primary (citable) accession number: P21866
Secondary accession number(s): P75739, P76822

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 120 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents