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P21865

- KDPD_ECOLI

UniProt

P21865 - KDPD_ECOLI

Protein

Sensor protein KdpD

Gene

kdpD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon. KdpD may function as a membrane-associated protein kinase that phosphorylates KdpE in response to environmental signals.1 Publication

    Catalytic activityi

    ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

    GO - Molecular functioni

    1. aspartate kinase activity Source: EcoCyc
    2. ATP binding Source: UniProtKB-KW
    3. phosphorelay sensor kinase activity Source: EcoCyc
    4. protein binding Source: IntAct

    GO - Biological processi

    1. detection of chemical stimulus Source: EcoCyc
    2. osmosensory signaling via phosphorelay pathway Source: EcoCyc
    3. protein autophosphorylation Source: EcoCyc
    4. signal transduction by phosphorylation Source: GOC

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Two-component regulatory system

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:KDPD-MONOMER.
    ECOL316407:JW0683-MONOMER.
    RETL1328306-WGS:GSTH-6122-MONOMER.
    BRENDAi2.7.13.3. 2026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sensor protein KdpD (EC:2.7.13.3)
    Gene namesi
    Name:kdpD
    Ordered Locus Names:b0695, JW0683
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10516. kdpD.

    Subcellular locationi

    Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. intrinsic component of the cytoplasmic side of the plasma membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 894894Sensor protein KdpDPRO_0000074772Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei673 – 6731Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP21865.
    PRIDEiP21865.

    Expressioni

    Gene expression databases

    GenevestigatoriP21865.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    kdpEP218665EBI-1123100,EBI-6403634
    ptsNP698294EBI-1123100,EBI-547017

    Protein-protein interaction databases

    DIPiDIP-10062N.
    IntActiP21865. 5 interactions.
    STRINGi511145.b0695.

    Structurei

    Secondary structure

    1
    894
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi399 – 4013
    Helixi402 – 42019
    Helixi430 – 44314
    Helixi450 – 46112
    Helixi475 – 49824

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KSFNMR-A397-502[»]
    ProteinModelPortaliP21865.
    SMRiP21865. Positions 22-227, 397-502, 599-882.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21865.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 400400CytoplasmicCuratedAdd
    BLAST
    Topological domaini424 – 4274PeriplasmicCurated
    Topological domaini445 – 4451CytoplasmicCurated
    Topological domaini467 – 4748PeriplasmicCurated
    Topological domaini499 – 894396CytoplasmicCuratedAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei401 – 42323HelicalCuratedAdd
    BLAST
    Transmembranei428 – 44417HelicalCuratedAdd
    BLAST
    Transmembranei446 – 46621HelicalCuratedAdd
    BLAST
    Transmembranei475 – 49824HelicalCuratedAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini670 – 883214Histidine kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the central section; belongs to the universal stress protein A family.Curated
    Contains 1 histidine kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2205.
    HOGENOMiHOG000082933.
    KOiK07646.
    OMAiETPRLHR.
    OrthoDBiEOG6G4VQG.
    PhylomeDBiP21865.

    Family and domain databases

    Gene3Di1.10.287.130. 1 hit.
    3.30.565.10. 1 hit.
    3.40.50.300. 1 hit.
    3.40.50.620. 1 hit.
    InterProiIPR003661. EnvZ-like_dim/P.
    IPR003018. GAF.
    IPR003594. HATPase_ATP-bd.
    IPR025201. KdpD_TM.
    IPR027417. P-loop_NTPase.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR005467. Sig_transdc_His_kinase_core.
    IPR009082. Sig_transdc_His_kinase_dimeric.
    IPR003852. Sig_transdc_His_kinase_KdpD_N.
    IPR006016. UspA.
    [Graphical view]
    PfamiPF13493. DUF4118. 1 hit.
    PF13492. GAF_3. 1 hit.
    PF02518. HATPase_c. 1 hit.
    PF00512. HisKA. 1 hit.
    PF02702. KdpD. 1 hit.
    PF00582. Usp. 1 hit.
    [Graphical view]
    PRINTSiPR00344. BCTRLSENSOR.
    SMARTiSM00387. HATPase_c. 1 hit.
    SM00388. HisKA. 1 hit.
    [Graphical view]
    SUPFAMiSSF47384. SSF47384. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEiPS50109. HIS_KIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21865-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNEPLRPDP DRLLEQTAAP HRGKLKVFFG ACAGVGKTWA MLAEAQRLRA    50
    QGLDIVVGVV ETHGRKDTAA MLEGLAVLPL KRQAYRGRHI SEFDLDAALA 100
    RRPALILMDE LAHSNAPGSR HPKRWQDIEE LLEAGIDVFT TVNVQHLESL 150
    NDVVSGVTGI QVRETVPDPF FDAADDVVLV DLPPDDLRQR LKEGKVYIAG 200
    QAERAIEHFF RKGNLIALRE LALRRTADRV DEQMRAWRGH PGEEKVWHTR 250
    DAILLCIGHN TGSEKLVRAA ARLASRLGSV WHAVYVETPA LHRLPEKKRR 300
    AILSALRLAQ ELGAETATLS DPAEEKAVVR YAREHNLGKI ILGRPASRRW 350
    WRRETFADRL ARIAPDLDQV LVALDEPPAR TINNAPDNRS FKDKWRVQIQ 400
    GCVVAAALCA VITLIAMQWL MAFDAANLVM LYLLGVVVVA LFYGRWPSVV 450
    ATVINVVSFD LFFIAPRGTL AVSDVQYLLT FAVMLTVGLV IGNLTAGVRY 500
    QARVARYREQ RTRHLYEMSK ALAVGRSPQD IAATSEQFIA STFHARSQVL 550
    LPDDNGKLQP LTHPQGMTPW DDAIAQWSFD KGLPAGAGTD TLPGVPYQIL 600
    PLKSGEKTYG LVVVEPGNLR QLMIPEQQRL LETFTLLVAN ALERLTLTAS 650
    EEQARMASER EQIRNALLAA LSHDLRTPLT VLFGQAEILT LDLASEGSPH 700
    ARQASEIRQH VLNTTRLVNN LLDMARIQSG GFNLKKEWLT LEEVVGSALQ 750
    MLEPGLSSPI NLSLPEPLTL IHVDGPLFER VLINLLENAV KYAGAQAEIG 800
    IDAHVEGENL QLDVWDNGPG LPPGQEQTIF DKFARGNKES AVPGVGLGLA 850
    ICRAIVDVHG GTITAFNRPE GGACFRVTLP QQTAPELEEF HEDM 894
    Length:894
    Mass (Da):98,718
    Last modified:February 1, 1996 - v2
    Checksum:iE9D2749B4CAA12DA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36066 Genomic DNA. Translation: AAA24041.1.
    U00096 Genomic DNA. Translation: AAC73789.1.
    AP009048 Genomic DNA. Translation: BAA35352.1.
    PIRiB42372.
    RefSeqiNP_415223.1. NC_000913.3.
    YP_488975.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73789; AAC73789; b0695.
    BAA35352; BAA35352; BAA35352.
    GeneIDi12930931.
    946744.
    KEGGiecj:Y75_p0674.
    eco:b0695.
    PATRICi32116585. VBIEscCol129921_0725.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36066 Genomic DNA. Translation: AAA24041.1 .
    U00096 Genomic DNA. Translation: AAC73789.1 .
    AP009048 Genomic DNA. Translation: BAA35352.1 .
    PIRi B42372.
    RefSeqi NP_415223.1. NC_000913.3.
    YP_488975.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2KSF NMR - A 397-502 [» ]
    ProteinModelPortali P21865.
    SMRi P21865. Positions 22-227, 397-502, 599-882.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10062N.
    IntActi P21865. 5 interactions.
    STRINGi 511145.b0695.

    Proteomic databases

    PaxDbi P21865.
    PRIDEi P21865.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73789 ; AAC73789 ; b0695 .
    BAA35352 ; BAA35352 ; BAA35352 .
    GeneIDi 12930931.
    946744.
    KEGGi ecj:Y75_p0674.
    eco:b0695.
    PATRICi 32116585. VBIEscCol129921_0725.

    Organism-specific databases

    EchoBASEi EB0511.
    EcoGenei EG10516. kdpD.

    Phylogenomic databases

    eggNOGi COG2205.
    HOGENOMi HOG000082933.
    KOi K07646.
    OMAi ETPRLHR.
    OrthoDBi EOG6G4VQG.
    PhylomeDBi P21865.

    Enzyme and pathway databases

    BioCyci EcoCyc:KDPD-MONOMER.
    ECOL316407:JW0683-MONOMER.
    RETL1328306-WGS:GSTH-6122-MONOMER.
    BRENDAi 2.7.13.3. 2026.

    Miscellaneous databases

    EvolutionaryTracei P21865.
    PROi P21865.

    Gene expression databases

    Genevestigatori P21865.

    Family and domain databases

    Gene3Di 1.10.287.130. 1 hit.
    3.30.565.10. 1 hit.
    3.40.50.300. 1 hit.
    3.40.50.620. 1 hit.
    InterProi IPR003661. EnvZ-like_dim/P.
    IPR003018. GAF.
    IPR003594. HATPase_ATP-bd.
    IPR025201. KdpD_TM.
    IPR027417. P-loop_NTPase.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR004358. Sig_transdc_His_kin-like_C.
    IPR005467. Sig_transdc_His_kinase_core.
    IPR009082. Sig_transdc_His_kinase_dimeric.
    IPR003852. Sig_transdc_His_kinase_KdpD_N.
    IPR006016. UspA.
    [Graphical view ]
    Pfami PF13493. DUF4118. 1 hit.
    PF13492. GAF_3. 1 hit.
    PF02518. HATPase_c. 1 hit.
    PF00512. HisKA. 1 hit.
    PF02702. KdpD. 1 hit.
    PF00582. Usp. 1 hit.
    [Graphical view ]
    PRINTSi PR00344. BCTRLSENSOR.
    SMARTi SM00387. HATPase_c. 1 hit.
    SM00388. HisKA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47384. SSF47384. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEi PS50109. HIS_KIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators."
      Walderhaug M.O., Polarek J.W., Voelkner P., Daniel J.M., Hesse J.E., Altendorf K., Epstein W.
      J. Bacteriol. 174:2152-2159(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Membrane topology analysis of the sensor kinase KdpD of Escherichia coli."
      Zimmann P., Puppe W., Altendorf K.
      J. Biol. Chem. 270:28282-28288(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.
    6. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiKDPD_ECOLI
    AccessioniPrimary (citable) accession number: P21865
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3