Sensor protein KdpD - Escherichia coli (strain K12)

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P21865 (KDPD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 126. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sensor protein KdpD
EC=2.7.13.3

Gene names

Name:	kdpD
Ordered Locus Names:	b0695, JW0683

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	894 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon. KdpD may function as a membrane-associated protein kinase that phosphorylates KdpE in response to environmental signals. Ref.1
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.1.
Sequence similarities	In the central section; belongs to the universal stress protein A family. Contains 1 histidine kinase domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	detection of chemical stimulus Inferred from direct assay PubMed 8276778. Source: EcoCyc osmosensory signaling via phosphorelay pathway Inferred from direct assay PubMed 8276778. Source: EcoCyc protein autophosphorylation Inferred from direct assay PubMed 8223625. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intrinsic to internal side of plasma membrane Inferred from direct assay Ref.1. Source: EcoCyc
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate kinase activity Inferred from direct assay PubMed 8276778. Source: EcoCyc phosphorelay sensor kinase activity Inferred from direct assay PubMed 8276778. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
itself		2	EBI-1123100,EBI-1123100
kdpE	P21866	4	EBI-1123100,EBI-6403634
ptsN	P69829	3	EBI-1123100,EBI-547017

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 894

894

Sensor protein KdpD

PRO_0000074772

Regions

Topological domain

1 – 400

400

Cytoplasmic Probable

Transmembrane

401 – 423

Helical; Probable

Topological domain

424 – 427

Periplasmic Probable

Transmembrane

428 – 444

Helical; Probable

Topological domain

445

Cytoplasmic Probable

Transmembrane

446 – 466

Helical; Probable

Topological domain

467 – 474

Periplasmic Probable

Transmembrane

475 – 498

Helical; Probable

Topological domain

499 – 894

396

Cytoplasmic Probable

Domain

670 – 883

214

Histidine kinase

Amino acid modifications

Modified residue

673

Phosphohistidine; by autocatalysis By similarity

Secondary structure

894

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P21865 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: E9D2749B4CAA12DA
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FASTA

894

98,718

        10         20         30         40         50         60 
MNNEPLRPDP DRLLEQTAAP HRGKLKVFFG ACAGVGKTWA MLAEAQRLRA QGLDIVVGVV 

        70         80         90        100        110        120 
ETHGRKDTAA MLEGLAVLPL KRQAYRGRHI SEFDLDAALA RRPALILMDE LAHSNAPGSR 

       130        140        150        160        170        180 
HPKRWQDIEE LLEAGIDVFT TVNVQHLESL NDVVSGVTGI QVRETVPDPF FDAADDVVLV 

       190        200        210        220        230        240 
DLPPDDLRQR LKEGKVYIAG QAERAIEHFF RKGNLIALRE LALRRTADRV DEQMRAWRGH 

       250        260        270        280        290        300 
PGEEKVWHTR DAILLCIGHN TGSEKLVRAA ARLASRLGSV WHAVYVETPA LHRLPEKKRR 

       310        320        330        340        350        360 
AILSALRLAQ ELGAETATLS DPAEEKAVVR YAREHNLGKI ILGRPASRRW WRRETFADRL 

       370        380        390        400        410        420 
ARIAPDLDQV LVALDEPPAR TINNAPDNRS FKDKWRVQIQ GCVVAAALCA VITLIAMQWL 

       430        440        450        460        470        480 
MAFDAANLVM LYLLGVVVVA LFYGRWPSVV ATVINVVSFD LFFIAPRGTL AVSDVQYLLT 

       490        500        510        520        530        540 
FAVMLTVGLV IGNLTAGVRY QARVARYREQ RTRHLYEMSK ALAVGRSPQD IAATSEQFIA 

       550        560        570        580        590        600 
STFHARSQVL LPDDNGKLQP LTHPQGMTPW DDAIAQWSFD KGLPAGAGTD TLPGVPYQIL 

       610        620        630        640        650        660 
PLKSGEKTYG LVVVEPGNLR QLMIPEQQRL LETFTLLVAN ALERLTLTAS EEQARMASER 

       670        680        690        700        710        720 
EQIRNALLAA LSHDLRTPLT VLFGQAEILT LDLASEGSPH ARQASEIRQH VLNTTRLVNN 

       730        740        750        760        770        780 
LLDMARIQSG GFNLKKEWLT LEEVVGSALQ MLEPGLSSPI NLSLPEPLTL IHVDGPLFER 

       790        800        810        820        830        840 
VLINLLENAV KYAGAQAEIG IDAHVEGENL QLDVWDNGPG LPPGQEQTIF DKFARGNKES 

       850        860        870        880        890 
AVPGVGLGLA ICRAIVDVHG GTITAFNRPE GGACFRVTLP QQTAPELEEF HEDM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators." Walderhaug M.O., Polarek J.W., Voelkner P., Daniel J.M., Hesse J.E., Altendorf K., Epstein W. J. Bacteriol. 174:2152-2159(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Membrane topology analysis of the sensor kinase KdpD of Escherichia coli." Zimmann P., Puppe W., Altendorf K. J. Biol. Chem. 270:28282-28288(1995) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M36066 Genomic DNA. Translation: AAA24041.1.
U00096 Genomic DNA. Translation: AAC73789.1.
AP009048 Genomic DNA. Translation: BAA35352.1.

PIR

B42372.

RefSeq

NP_415223.1. NC_000913.2.
YP_488975.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KSF	NMR	-	A	397-502	[»]

ProteinModelPortal

P21865.

SMR

P21865. Positions 22-227, 397-502, 661-884.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10062N.

IntAct

P21865. 5 interactions.

STRING

511145.b0695.

Proteomic databases

PaxDb

P21865.

PRIDE

P21865.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73789; AAC73789; b0695.
BAA35352; BAA35352; BAA35352.

GeneID

12930931.
946744.

KEGG

ecj:Y75_p0674.
eco:b0695.

PATRIC

32116585. VBIEscCol129921_0725.

Organism-specific databases

EchoBASE

EB0511.

EcoGene

EG10516. kdpD.

Phylogenomic databases

eggNOG

COG2205.

HOGENOM

HOG000082933.

K07646.

OMA

HAPWTAL.

ProtClustDB

PRK10490.

Enzyme and pathway databases

BioCyc

EcoCyc:KDPD-MONOMER.
ECOL316407:JW0683-MONOMER.

BRENDA

2.7.13.3. 2026.

Gene expression databases

Genevestigator

P21865.

Family and domain databases

Gene3D

1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
3.40.50.620. 1 hit.

InterPro

IPR025201. DUF4118.
IPR003018. GAF.
IPR003594. HATPase_ATP-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR003661. Sig_transdc_His_kin_sub1_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR003852. Sig_transdc_His_kinase_KdpD_N.
IPR006016. UspA.
[Graphical view]

Pfam

PF13493. DUF4118. 1 hit.
PF13492. GAF_3. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF02702. KdpD. 1 hit.
PF00582. Usp. 1 hit.
[Graphical view]

PRINTS

PR00344. BCTRLSENSOR.

SMART

SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]

SUPFAM

SSF55874. ATP_bd_ATPase. 1 hit.
SSF47384. His_kin_homodim. 1 hit.

PROSITE

PS50109. HIS_KIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P21865.

Entry information

Entry name

KDPD_ECOLI

Accession

Primary (citable) accession number: P21865

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 126 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents