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Protein

Sensor protein KdpD

Gene

kdpD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon. KdpD may function as a membrane-associated protein kinase that phosphorylates KdpE in response to environmental signals.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • aspartate kinase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • detection of chemical stimulus Source: EcoCyc
  • osmosensory signaling via phosphorelay pathway Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:KDPD-MONOMER.
ECOL316407:JW0683-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein KdpD (EC:2.7.13.3)
Gene namesi
Name:kdpD
Ordered Locus Names:b0695, JW0683
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10516. kdpD.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 400CytoplasmicCuratedAdd BLAST400
Transmembranei401 – 423HelicalCuratedAdd BLAST23
Topological domaini424 – 427PeriplasmicCurated4
Transmembranei428 – 444HelicalCuratedAdd BLAST17
Topological domaini445CytoplasmicCurated1
Transmembranei446 – 466HelicalCuratedAdd BLAST21
Topological domaini467 – 474PeriplasmicCurated8
Transmembranei475 – 498HelicalCuratedAdd BLAST24
Topological domaini499 – 894CytoplasmicCuratedAdd BLAST396

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • intrinsic component of the cytoplasmic side of the plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747721 – 894Sensor protein KdpDAdd BLAST894

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei673Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21865.
PRIDEiP21865.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
kdpEP218665EBI-1123100,EBI-6403634
ptsNP698294EBI-1123100,EBI-547017

Protein-protein interaction databases

BioGridi4259925. 7 interactors.
DIPiDIP-10062N.
IntActiP21865. 5 interactors.
STRINGi511145.b0695.

Structurei

Secondary structure

1894
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi399 – 401Combined sources3
Helixi402 – 420Combined sources19
Helixi430 – 443Combined sources14
Helixi450 – 461Combined sources12
Helixi475 – 498Combined sources24
Helixi515 – 523Combined sources9
Helixi528 – 543Combined sources16
Beta strandi545 – 552Combined sources8
Helixi572 – 581Combined sources10
Beta strandi589 – 592Combined sources4
Beta strandi595 – 604Combined sources10
Beta strandi607 – 617Combined sources11
Helixi619 – 622Combined sources4
Helixi625 – 646Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KSFNMR-A397-502[»]
4QPRX-ray1.55A515-646[»]
4Y2FX-ray1.40A515-646[»]
ProteinModelPortaliP21865.
SMRiP21865.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21865.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini670 – 883Histidine kinasePROSITE-ProRule annotationAdd BLAST214

Sequence similaritiesi

In the central section; belongs to the universal stress protein A family.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C08. Bacteria.
COG2205. LUCA.
HOGENOMiHOG000082933.
InParanoidiP21865.
KOiK07646.
OMAiLWATAFQ.
PhylomeDBiP21865.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR003018. GAF.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR025201. KdpD_TM.
IPR027417. P-loop_NTPase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR003852. Sig_transdc_His_kinase_KdpD_N.
[Graphical view]
PfamiPF13493. DUF4118. 1 hit.
PF13492. GAF_3. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF02702. KdpD. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNEPLRPDP DRLLEQTAAP HRGKLKVFFG ACAGVGKTWA MLAEAQRLRA
60 70 80 90 100
QGLDIVVGVV ETHGRKDTAA MLEGLAVLPL KRQAYRGRHI SEFDLDAALA
110 120 130 140 150
RRPALILMDE LAHSNAPGSR HPKRWQDIEE LLEAGIDVFT TVNVQHLESL
160 170 180 190 200
NDVVSGVTGI QVRETVPDPF FDAADDVVLV DLPPDDLRQR LKEGKVYIAG
210 220 230 240 250
QAERAIEHFF RKGNLIALRE LALRRTADRV DEQMRAWRGH PGEEKVWHTR
260 270 280 290 300
DAILLCIGHN TGSEKLVRAA ARLASRLGSV WHAVYVETPA LHRLPEKKRR
310 320 330 340 350
AILSALRLAQ ELGAETATLS DPAEEKAVVR YAREHNLGKI ILGRPASRRW
360 370 380 390 400
WRRETFADRL ARIAPDLDQV LVALDEPPAR TINNAPDNRS FKDKWRVQIQ
410 420 430 440 450
GCVVAAALCA VITLIAMQWL MAFDAANLVM LYLLGVVVVA LFYGRWPSVV
460 470 480 490 500
ATVINVVSFD LFFIAPRGTL AVSDVQYLLT FAVMLTVGLV IGNLTAGVRY
510 520 530 540 550
QARVARYREQ RTRHLYEMSK ALAVGRSPQD IAATSEQFIA STFHARSQVL
560 570 580 590 600
LPDDNGKLQP LTHPQGMTPW DDAIAQWSFD KGLPAGAGTD TLPGVPYQIL
610 620 630 640 650
PLKSGEKTYG LVVVEPGNLR QLMIPEQQRL LETFTLLVAN ALERLTLTAS
660 670 680 690 700
EEQARMASER EQIRNALLAA LSHDLRTPLT VLFGQAEILT LDLASEGSPH
710 720 730 740 750
ARQASEIRQH VLNTTRLVNN LLDMARIQSG GFNLKKEWLT LEEVVGSALQ
760 770 780 790 800
MLEPGLSSPI NLSLPEPLTL IHVDGPLFER VLINLLENAV KYAGAQAEIG
810 820 830 840 850
IDAHVEGENL QLDVWDNGPG LPPGQEQTIF DKFARGNKES AVPGVGLGLA
860 870 880 890
ICRAIVDVHG GTITAFNRPE GGACFRVTLP QQTAPELEEF HEDM
Length:894
Mass (Da):98,718
Last modified:February 1, 1996 - v2
Checksum:iE9D2749B4CAA12DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36066 Genomic DNA. Translation: AAA24041.1.
U00096 Genomic DNA. Translation: AAC73789.1.
AP009048 Genomic DNA. Translation: BAA35352.1.
PIRiB42372.
RefSeqiNP_415223.1. NC_000913.3.
WP_001310640.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73789; AAC73789; b0695.
BAA35352; BAA35352; BAA35352.
GeneIDi946744.
KEGGiecj:JW0683.
eco:b0695.
PATRICi32116585. VBIEscCol129921_0725.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36066 Genomic DNA. Translation: AAA24041.1.
U00096 Genomic DNA. Translation: AAC73789.1.
AP009048 Genomic DNA. Translation: BAA35352.1.
PIRiB42372.
RefSeqiNP_415223.1. NC_000913.3.
WP_001310640.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KSFNMR-A397-502[»]
4QPRX-ray1.55A515-646[»]
4Y2FX-ray1.40A515-646[»]
ProteinModelPortaliP21865.
SMRiP21865.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259925. 7 interactors.
DIPiDIP-10062N.
IntActiP21865. 5 interactors.
STRINGi511145.b0695.

Proteomic databases

PaxDbiP21865.
PRIDEiP21865.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73789; AAC73789; b0695.
BAA35352; BAA35352; BAA35352.
GeneIDi946744.
KEGGiecj:JW0683.
eco:b0695.
PATRICi32116585. VBIEscCol129921_0725.

Organism-specific databases

EchoBASEiEB0511.
EcoGeneiEG10516. kdpD.

Phylogenomic databases

eggNOGiENOG4105C08. Bacteria.
COG2205. LUCA.
HOGENOMiHOG000082933.
InParanoidiP21865.
KOiK07646.
OMAiLWATAFQ.
PhylomeDBiP21865.

Enzyme and pathway databases

BioCyciEcoCyc:KDPD-MONOMER.
ECOL316407:JW0683-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

EvolutionaryTraceiP21865.
PROiP21865.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR003018. GAF.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR025201. KdpD_TM.
IPR027417. P-loop_NTPase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR003852. Sig_transdc_His_kinase_KdpD_N.
[Graphical view]
PfamiPF13493. DUF4118. 1 hit.
PF13492. GAF_3. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF02702. KdpD. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKDPD_ECOLI
AccessioniPrimary (citable) accession number: P21865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.