Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sensor protein KdpD

Gene

kdpD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system KdpD/KdpE involved in the regulation of the kdp operon. KdpD may function as a membrane-associated protein kinase that phosphorylates KdpE in response to environmental signals.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  1. aspartate kinase activity Source: EcoCyc
  2. ATP binding Source: UniProtKB-KW
  3. phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  1. detection of chemical stimulus Source: EcoCyc
  2. osmosensory signaling via phosphorelay pathway Source: EcoCyc
  3. peptidyl-histidine phosphorylation Source: GO_Central
  4. protein autophosphorylation Source: EcoCyc
  5. signal transduction by phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:KDPD-MONOMER.
ECOL316407:JW0683-MONOMER.
RETL1328306-WGS:GSTH-6122-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein KdpD (EC:2.7.13.3)
Gene namesi
Name:kdpD
Ordered Locus Names:b0695, JW0683
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10516. kdpD.

Subcellular locationi

  1. Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 400400CytoplasmicCuratedAdd
BLAST
Transmembranei401 – 42323HelicalCuratedAdd
BLAST
Topological domaini424 – 4274PeriplasmicCurated
Transmembranei428 – 44417HelicalCuratedAdd
BLAST
Topological domaini445 – 4451CytoplasmicCurated
Transmembranei446 – 46621HelicalCuratedAdd
BLAST
Topological domaini467 – 4748PeriplasmicCurated
Transmembranei475 – 49824HelicalCuratedAdd
BLAST
Topological domaini499 – 894396CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. intrinsic component of the cytoplasmic side of the plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 894894Sensor protein KdpDPRO_0000074772Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei673 – 6731Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21865.
PRIDEiP21865.

Expressioni

Gene expression databases

GenevestigatoriP21865.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
kdpEP218665EBI-1123100,EBI-6403634
ptsNP698294EBI-1123100,EBI-547017

Protein-protein interaction databases

DIPiDIP-10062N.
IntActiP21865. 5 interactions.
STRINGi511145.b0695.

Structurei

Secondary structure

1
894
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi399 – 4013Combined sources
Helixi402 – 42019Combined sources
Helixi430 – 44314Combined sources
Helixi450 – 46112Combined sources
Helixi475 – 49824Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KSFNMR-A397-502[»]
ProteinModelPortaliP21865.
SMRiP21865. Positions 22-227, 397-502, 614-882.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21865.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini670 – 883214Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the central section; belongs to the universal stress protein A family.Curated
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2205.
HOGENOMiHOG000082933.
InParanoidiP21865.
KOiK07646.
OMAiLWATAFQ.
OrthoDBiEOG6G4VQG.
PhylomeDBiP21865.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR003661. EnvZ-like_dim/P.
IPR003018. GAF.
IPR003594. HATPase_C.
IPR025201. KdpD_TM.
IPR027417. P-loop_NTPase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR003852. Sig_transdc_His_kinase_KdpD_N.
IPR006016. UspA.
[Graphical view]
PfamiPF13493. DUF4118. 1 hit.
PF13492. GAF_3. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF02702. KdpD. 1 hit.
PF00582. Usp. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNEPLRPDP DRLLEQTAAP HRGKLKVFFG ACAGVGKTWA MLAEAQRLRA
60 70 80 90 100
QGLDIVVGVV ETHGRKDTAA MLEGLAVLPL KRQAYRGRHI SEFDLDAALA
110 120 130 140 150
RRPALILMDE LAHSNAPGSR HPKRWQDIEE LLEAGIDVFT TVNVQHLESL
160 170 180 190 200
NDVVSGVTGI QVRETVPDPF FDAADDVVLV DLPPDDLRQR LKEGKVYIAG
210 220 230 240 250
QAERAIEHFF RKGNLIALRE LALRRTADRV DEQMRAWRGH PGEEKVWHTR
260 270 280 290 300
DAILLCIGHN TGSEKLVRAA ARLASRLGSV WHAVYVETPA LHRLPEKKRR
310 320 330 340 350
AILSALRLAQ ELGAETATLS DPAEEKAVVR YAREHNLGKI ILGRPASRRW
360 370 380 390 400
WRRETFADRL ARIAPDLDQV LVALDEPPAR TINNAPDNRS FKDKWRVQIQ
410 420 430 440 450
GCVVAAALCA VITLIAMQWL MAFDAANLVM LYLLGVVVVA LFYGRWPSVV
460 470 480 490 500
ATVINVVSFD LFFIAPRGTL AVSDVQYLLT FAVMLTVGLV IGNLTAGVRY
510 520 530 540 550
QARVARYREQ RTRHLYEMSK ALAVGRSPQD IAATSEQFIA STFHARSQVL
560 570 580 590 600
LPDDNGKLQP LTHPQGMTPW DDAIAQWSFD KGLPAGAGTD TLPGVPYQIL
610 620 630 640 650
PLKSGEKTYG LVVVEPGNLR QLMIPEQQRL LETFTLLVAN ALERLTLTAS
660 670 680 690 700
EEQARMASER EQIRNALLAA LSHDLRTPLT VLFGQAEILT LDLASEGSPH
710 720 730 740 750
ARQASEIRQH VLNTTRLVNN LLDMARIQSG GFNLKKEWLT LEEVVGSALQ
760 770 780 790 800
MLEPGLSSPI NLSLPEPLTL IHVDGPLFER VLINLLENAV KYAGAQAEIG
810 820 830 840 850
IDAHVEGENL QLDVWDNGPG LPPGQEQTIF DKFARGNKES AVPGVGLGLA
860 870 880 890
ICRAIVDVHG GTITAFNRPE GGACFRVTLP QQTAPELEEF HEDM
Length:894
Mass (Da):98,718
Last modified:February 1, 1996 - v2
Checksum:iE9D2749B4CAA12DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36066 Genomic DNA. Translation: AAA24041.1.
U00096 Genomic DNA. Translation: AAC73789.1.
AP009048 Genomic DNA. Translation: BAA35352.1.
PIRiB42372.
RefSeqiNP_415223.1. NC_000913.3.
YP_488975.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73789; AAC73789; b0695.
BAA35352; BAA35352; BAA35352.
GeneIDi12930931.
946744.
KEGGiecj:Y75_p0674.
eco:b0695.
PATRICi32116585. VBIEscCol129921_0725.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36066 Genomic DNA. Translation: AAA24041.1.
U00096 Genomic DNA. Translation: AAC73789.1.
AP009048 Genomic DNA. Translation: BAA35352.1.
PIRiB42372.
RefSeqiNP_415223.1. NC_000913.3.
YP_488975.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KSFNMR-A397-502[»]
ProteinModelPortaliP21865.
SMRiP21865. Positions 22-227, 397-502, 614-882.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-10062N.
IntActiP21865. 5 interactions.
STRINGi511145.b0695.

Proteomic databases

PaxDbiP21865.
PRIDEiP21865.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73789; AAC73789; b0695.
BAA35352; BAA35352; BAA35352.
GeneIDi12930931.
946744.
KEGGiecj:Y75_p0674.
eco:b0695.
PATRICi32116585. VBIEscCol129921_0725.

Organism-specific databases

EchoBASEiEB0511.
EcoGeneiEG10516. kdpD.

Phylogenomic databases

eggNOGiCOG2205.
HOGENOMiHOG000082933.
InParanoidiP21865.
KOiK07646.
OMAiLWATAFQ.
OrthoDBiEOG6G4VQG.
PhylomeDBiP21865.

Enzyme and pathway databases

BioCyciEcoCyc:KDPD-MONOMER.
ECOL316407:JW0683-MONOMER.
RETL1328306-WGS:GSTH-6122-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

EvolutionaryTraceiP21865.
PROiP21865.

Gene expression databases

GenevestigatoriP21865.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
3.40.50.300. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR003661. EnvZ-like_dim/P.
IPR003018. GAF.
IPR003594. HATPase_C.
IPR025201. KdpD_TM.
IPR027417. P-loop_NTPase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
IPR003852. Sig_transdc_His_kinase_KdpD_N.
IPR006016. UspA.
[Graphical view]
PfamiPF13493. DUF4118. 1 hit.
PF13492. GAF_3. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF02702. KdpD. 1 hit.
PF00582. Usp. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "KdpD and KdpE, proteins that control expression of the kdpABC operon, are members of the two-component sensor-effector class of regulators."
    Walderhaug M.O., Polarek J.W., Voelkner P., Daniel J.M., Hesse J.E., Altendorf K., Epstein W.
    J. Bacteriol. 174:2152-2159(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Membrane topology analysis of the sensor kinase KdpD of Escherichia coli."
    Zimmann P., Puppe W., Altendorf K.
    J. Biol. Chem. 270:28282-28288(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiKDPD_ECOLI
AccessioniPrimary (citable) accession number: P21865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: February 1, 1996
Last modified: February 4, 2015
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.