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Protein

Rab GDP dissociation inhibitor alpha

Gene

GDI1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the GDP/GTP exchange reaction of most Rab proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation. Promotes the dissociation of RAB1A, RAB3A, RAB5A and RAB10 from membranes.2 Publications

GO - Molecular functioni

GO - Biological processi

  • negative regulation of axonogenesis Source: UniProtKB
  • negative regulation of protein targeting to membrane Source: UniProtKB
  • protein transport Source: InterPro
  • Rab protein signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-BTA-194840. Rho GTPase cycle.
R-BTA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Rab GDP dissociation inhibitor alpha
Short name:
Rab GDI alpha
Alternative name(s):
Guanosine diphosphate dissociation inhibitor 1
Short name:
GDI-1
SMG p25A GDI
Gene namesi
Name:GDI1
Synonyms:RABGDIA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome X

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • Golgi apparatus Source: UniProtKB-SubCell
  • midbody Source: Ensembl
  • myelin sheath Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi89K → A: Strongly reduces dissociation of RAB3A from the membrane. 1 Publication1
Mutagenesisi431F → E: Strongly reduces dissociation of RAB3A from the membrane. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000566691 – 447Rab GDP dissociation inhibitor alphaAdd BLAST447

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei112N6-acetyllysineBy similarity1
Modified residuei269N6-acetyllysineBy similarity1
Modified residuei427PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP21856.
PeptideAtlasiP21856.
PRIDEiP21856.

Expressioni

Gene expression databases

BgeeiENSBTAG00000012125.

Interactioni

Subunit structurei

Interacts with RHOH. Interacts with RAB1A, RAB5A and RAB10 (By similarity). Interacts with RAB3A.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-82N.
IntActiP21856. 1 interactor.
STRINGi9913.ENSBTAP00000016093.

Structurei

Secondary structure

1447
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Helixi14 – 25Combined sources12
Beta strandi30 – 33Combined sources4
Beta strandi35 – 39Combined sources5
Helixi41 – 43Combined sources3
Helixi49 – 55Combined sources7
Helixi64 – 66Combined sources3
Helixi69 – 71Combined sources3
Beta strandi74 – 77Combined sources4
Beta strandi80 – 82Combined sources3
Helixi86 – 94Combined sources9
Helixi96 – 99Combined sources4
Beta strandi102 – 104Combined sources3
Beta strandi107 – 112Combined sources6
Beta strandi115 – 118Combined sources4
Helixi123 – 128Combined sources6
Beta strandi130 – 132Combined sources3
Helixi134 – 149Combined sources16
Helixi155 – 158Combined sources4
Turni163 – 165Combined sources3
Helixi168 – 174Combined sources7
Helixi179 – 188Combined sources10
Beta strandi193 – 195Combined sources3
Helixi196 – 199Combined sources4
Beta strandi200 – 202Combined sources3
Helixi203 – 214Combined sources12
Beta strandi218 – 220Combined sources3
Beta strandi223 – 227Combined sources5
Helixi233 – 245Combined sources13
Beta strandi248 – 252Combined sources5
Beta strandi257 – 261Combined sources5
Beta strandi264 – 270Combined sources7
Beta strandi273 – 276Combined sources4
Beta strandi278 – 282Combined sources5
Helixi284 – 286Combined sources3
Helixi288 – 290Combined sources3
Beta strandi291 – 306Combined sources16
Beta strandi311 – 313Combined sources3
Beta strandi315 – 321Combined sources7
Helixi323 – 325Combined sources3
Beta strandi332 – 338Combined sources7
Helixi339 – 341Combined sources3
Beta strandi349 – 356Combined sources8
Helixi362 – 365Combined sources4
Helixi367 – 370Combined sources4
Turni371 – 373Combined sources3
Beta strandi377 – 390Combined sources14
Turni394 – 396Combined sources3
Beta strandi398 – 400Combined sources3
Beta strandi408 – 410Combined sources3
Helixi411 – 425Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D5TX-ray1.04A1-431[»]
1GNDX-ray1.81A1-447[»]
1LV0X-ray2.00A1-447[»]
ProteinModelPortaliP21856.
SMRiP21856.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21856.

Family & Domainsi

Sequence similaritiesi

Belongs to the Rab GDI family.Curated

Phylogenomic databases

eggNOGiKOG1439. Eukaryota.
COG5044. LUCA.
GeneTreeiENSGT00530000063044.
HOGENOMiHOG000163825.
HOVERGENiHBG000839.
InParanoidiP21856.
KOiK17255.
OMAiYCDPTYV.
OrthoDBiEOG091G06PV.
TreeFamiTF300449.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR018203. GDP_dissociation_inhibitor.
IPR016040. NAD(P)-bd_dom.
IPR000806. RabGDI.
[Graphical view]
PfamiPF00996. GDI. 1 hit.
[Graphical view]
PRINTSiPR00892. RABGDI.
PR00891. RABGDIREP.
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

P21856-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEEYDVIVL GTGLTECILS GIMSVNGKKV LHMDRNPYYG GESSSITPLE
60 70 80 90 100
ELYKRFQLLE GPPETMGRGR DWNVDLIPKF LMANGQLVKM LLYTEVTRYL
110 120 130 140 150
DFKVVEGSFV YKGGKIYKVP STETEALASN LMGMFEKRRF RKFLVFVANF
160 170 180 190 200
DENDPKTFEG VDPQNTSMRD VYRKFDLGQD VIDFTGHALA LYRTDDYLDQ
210 220 230 240 250
PCLETINRIK LYSESLARYG KSPYLYPLYG LGELPQGFAR LSAIYGGTYM
260 270 280 290 300
LNKPVDDIIM ENGKVVGVKS EGEVARCKQL ICDPSYVPDR VRKAGQVIRI
310 320 330 340 350
ICILSHPIKN TNDANSCQII IPQNQVNRKS DIYVCMISYA HNVAAQGKYI
360 370 380 390 400
AIASTTVETT DPEKEVEPAL ELLEPIDQKF VAISDLYEPI DDGSESQVFC
410 420 430 440
SCSYDATTHF ETTCNDIKDI YKRMAGSAFD FENMKRKQND VFGEADQ
Length:447
Mass (Da):50,566
Last modified:May 1, 1991 - v1
Checksum:i69C68B03BDC1BA3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90103 mRNA. Translation: BAA14134.1.
BC123440 mRNA. Translation: AAI23441.1.
PIRiA35652.
RefSeqiNP_776489.1. NM_174064.3.
UniGeneiBt.2235.

Genome annotation databases

EnsembliENSBTAT00000016093; ENSBTAP00000016093; ENSBTAG00000012125.
GeneIDi281188.
KEGGibta:281188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90103 mRNA. Translation: BAA14134.1.
BC123440 mRNA. Translation: AAI23441.1.
PIRiA35652.
RefSeqiNP_776489.1. NM_174064.3.
UniGeneiBt.2235.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D5TX-ray1.04A1-431[»]
1GNDX-ray1.81A1-447[»]
1LV0X-ray2.00A1-447[»]
ProteinModelPortaliP21856.
SMRiP21856.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-82N.
IntActiP21856. 1 interactor.
STRINGi9913.ENSBTAP00000016093.

Proteomic databases

PaxDbiP21856.
PeptideAtlasiP21856.
PRIDEiP21856.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000016093; ENSBTAP00000016093; ENSBTAG00000012125.
GeneIDi281188.
KEGGibta:281188.

Organism-specific databases

CTDi2664.

Phylogenomic databases

eggNOGiKOG1439. Eukaryota.
COG5044. LUCA.
GeneTreeiENSGT00530000063044.
HOGENOMiHOG000163825.
HOVERGENiHBG000839.
InParanoidiP21856.
KOiK17255.
OMAiYCDPTYV.
OrthoDBiEOG091G06PV.
TreeFamiTF300449.

Enzyme and pathway databases

ReactomeiR-BTA-194840. Rho GTPase cycle.
R-BTA-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

EvolutionaryTraceiP21856.

Gene expression databases

BgeeiENSBTAG00000012125.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR018203. GDP_dissociation_inhibitor.
IPR016040. NAD(P)-bd_dom.
IPR000806. RabGDI.
[Graphical view]
PfamiPF00996. GDI. 1 hit.
[Graphical view]
PRINTSiPR00892. RABGDI.
PR00891. RABGDIREP.
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGDIA_BOVIN
AccessioniPrimary (citable) accession number: P21856
Secondary accession number(s): A4FUY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.