Reviewed, UniProtKB/Swiss-Prot P21845 (TRYB2_MOUSE)

Names and origin

Protein names

Recommended name:
    Tryptase beta-2
      Short name=Tryptase-2
    EC=3.4.21.59
Alternative name(s):
    Mast cell protease 6
      Short name=mMCP-6

Gene names

Name:	Tpsb2
Synonyms:	Mcpt6

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Has an immunoprotective role during bacterial infection. Required to efficiently combat K.pneumoniae infection. Ref.6
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.
Subunit structure	Homotetramer By similarity.
Subcellular location	Secreted By similarity. Note: Released from the secretory granules upon mast cell activation By similarity.
Tissue specificity	During embryogenesis, detected primarily in skin. Ref.8
Developmental stage	Not detected at early embryonic stages but is abundantly expressed in later stages with a peak at E17.5-E18.5. Ref.8
Disruption phenotype	Mice are unable to efficiently combat K.pneumoniae infection of the peritoneal cavity. Ref.6
Sequence similarities	Belongs to the peptidase S1 family. Tryptase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Phosphoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	inflammatory response Traceable author statement. Source: MGI proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Traceable author statement. Source: MGI
Molecular function	heparin binding Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction. Source: MGI serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P21845-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P21845-2)

The sequence of this isoform differs from the canonical sequence as follows:
     223-230: GDSGGPLV → PFCIGDDI
     231-276: Missing.

Note: Probably non functional.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Propeptide	22 – 31	10	Activation peptide Ref.5		PRO_0000027490
	Chain	32 – 276	245	Tryptase beta-2		PRO_0000027491
Regions
	Domain	32 – 273	242	Peptidase S1
Sites
	Active site	75	1	Charge relay system By similarity
	Active site	122	1	Charge relay system By similarity
	Active site	225	1	Charge relay system By similarity
Amino acid modifications
	Modified residue	98	1	Phosphotyrosine Ref.7
	Glycosylation	133	1	N-linked (GlcNAc...) Potential
	Disulfide bond	60 ↔ 76		By similarity
	Disulfide bond	156 ↔ 231		By similarity
	Disulfide bond	189 ↔ 212		By similarity
	Disulfide bond	221 ↔ 249		By similarity
Natural variations
	Alternative sequence	223 – 230	8	GDSGGPLV → PFCIGDDI in isoform Short.		VSP_005376
	Alternative sequence	231 – 276	46	Missing in isoform Short.		VSP_005377

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified August 1, 1991. Version 2. Checksum: 525B2C9A04A72200	FASTA	276	30,927
	10 20 30 40 50 60 MLKRRLLLLW ALSLLASLVY SAPRPANQRV GIVGGHEASE SKWPWQVSLR FKLNYWIHFC 70 80 90 100 110 120 GGSLIHPQWV LTAAHCVGPH IKSPQLFRVQ LREQYLYYGD QLLSLNRIVV HPHYYTAEGG 130 140 150 160 170 180 ADVALLELEV PVNVSTHIHP ISLPPASETF PPGTSCWVTG WGDIDNDEPL PPPYPLKQVK 190 200 210 220 230 240 VPIVENSLCD RKYHTGLYTG DDFPIVHDGM LCAGNTRRDS CQGDSGGPLV CKVKGTWLQA 250 260 270 GVVSWGEGCA QPNKPGIYTR VTYYLDWIHR YVPEHS
	Isoform Short. Checksum: 503F4E4477EA7073	FASTA	230	25,829
	10 20 30 40 50 60 MLKRRLLLLW ALSLLASLVY SAPRPANQRV GIVGGHEASE SKWPWQVSLR FKLNYWIHFC 70 80 90 100 110 120 GGSLIHPQWV LTAAHCVGPH IKSPQLFRVQ LREQYLYYGD QLLSLNRIVV HPHYYTAEGG 130 140 150 160 170 180 ADVALLELEV PVNVSTHIHP ISLPPASETF PPGTSCWVTG WGDIDNDEPL PPPYPLKQVK 190 200 210 220 230 VPIVENSLCD RKYHTGLYTG DDFPIVHDGM LCAGNTRRDS CQPFCIGDDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the cDNA and gene of mouse mast cell protease-6. Transcription by progenitor mast cells and mast cells of the connective tissue subclass." Reynolds D.S., Gurley D.S., Austen K.F., Serafin W.E. J. Biol. Chem. 266:3847-3853(1991) [PubMed: 1995638] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Expression of a mast cell tryptase in the human monocytic cell lines U-937 and Mono Mac 6." Huang R., Abrink M., Gobl A.E., Nilsson G., Aveskogh M., Larsson L.G., Nilsson K., Hellman L. Scand. J. Immunol. 38:359-367(1993) [PubMed: 8210998] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Leaden X A1.
[3]	"Genes for mast-cell serine protease and their molecular evolution." Huang R., Hellman L.T. Immunogenetics 40:397-414(1994) [PubMed: 7959952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). Strain: Leaden X A1.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Strain: FVB/N. Tissue: Colon.
[5]	"Different mouse mast cell populations express various combinations of at least six distinct mast cell serine proteases." Reynolds D.S., Stevens R.L., Lane W.S., Carr M.H., Austen K.F., Serafin W.E. Proc. Natl. Acad. Sci. U.S.A. 87:3230-3234(1990) [PubMed: 2326280] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-54.
[6]	"The mast cell-restricted tryptase mMCP-6 has a critical immunoprotective role in bacterial infections." Thakurdas S.M., Melicoff E., Sansores-Garcia L., Moreira D.C., Petrova Y., Stevens R.L., Adachi R. J. Biol. Chem. 282:20809-20815(2007) [PubMed: 17456473] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed: 17947660] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-98, MASS SPECTROMETRY. Tissue: Mast cell.
[8]	"The expression pattern of three mast cell specific proteases during mouse development." Abraham D., Oster H., Huber M., Leitges M. Mol. Immunol. 44:732-740(2007) [PubMed: 16777228] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M57626 mRNA. Translation: AAA39988.1. M57625 mRNA. Translation: AAA39987.1. L31853 mRNA. Translation: AAA39725.1. X78542 mRNA. Translation: CAA55288.1. BC024374 mRNA. Translation: AAH24374.1.
IPI	IPI00128610. IPI00228492.
PIR	A38654. I48685.
UniGene	Mm.7409
3D structure databases
HSSP	HSSP built from PDB template 1A0L based on UniProtKB P20231.
SMR	P21845. Positions 32-274.
ModBase	Search...
Protein family/group databases
MEROPS	S01.025.
PTM databases
PhosphoSite	P21845.
Genome annotation databases
Ensembl	ENSMUSG00000033825. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:96942. Tpsb2.
Phylogenomic databases
HOGENOM	P21845.
HOVERGEN	P21845.
Enzyme and pathway databases
BRENDA	3.4.21.59. 244.
Gene expression databases
ArrayExpress	P21845.
Bgee	P21845.
CleanEx	MM_TPSB2.
GermOnline	ENSMUSG00000033825. Mus musculus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

TRYB2_MOUSE

Accession

Primary (citable) accession number: P21845
Secondary accession number(s): Q61962

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	August 1, 1991
Last modified:	May 26, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)