Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chymase

Gene

Cma1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

Catalytic activityi

Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei66Charge relay systemBy similarity1
Active sitei110Charge relay systemBy similarity1
Active sitei203Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.B5 3474
ReactomeiR-MMU-1433557 Signaling by SCF-KIT
R-MMU-1592389 Activation of Matrix Metalloproteinases
R-MMU-2022377 Metabolism of Angiotensinogen to Angiotensins

Protein family/group databases

MEROPSiS01.150

Names & Taxonomyi

Protein namesi
Recommended name:
Chymase (EC:3.4.21.39)
Alternative name(s):
Alpha-chymase
Mast cell chymase 1
Mast cell protease 5
Short name:
mMCP-5
Mast cell protease I
Gene namesi
Name:Cma1
Synonyms:Mcpt5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:96941 Cma1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000002745520 – 21Activation peptide1 Publication2
ChainiPRO_000002745622 – 247ChymaseAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 67PROSITE-ProRule annotation
Glycosylationi80N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi144 ↔ 209PROSITE-ProRule annotation
Disulfide bondi175 ↔ 188PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP21844
PaxDbiP21844
PRIDEiP21844

PTM databases

iPTMnetiP21844
PhosphoSitePlusiP21844

Expressioni

Tissue specificityi

Mast cells.

Gene expression databases

CleanExiMM_CMA1
ExpressionAtlasiP21844 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022834

Structurei

3D structure databases

ProteinModelPortaliP21844
SMRiP21844
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 245Peptidase S1PROSITE-ProRule annotationAdd BLAST224

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118895
HOVERGENiHBG013304
InParanoidiP21844
KOiK01329

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21844-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLLTLHLLL LLLGSSTKAG EIIGGTECIP HSRPYMAYLE IVTSENYLSA
60 70 80 90 100
CSGFLIRRNF VLTAAHCAGR SITVLLGAHN KTSKEDTWQK LEVEKQFLHP
110 120 130 140 150
KYDENLVVHD IMLLKLKEKA KLTLGVGTLP LSANFNFIPP GRMCRAVGWG
160 170 180 190 200
RTNVNEPASD TLQEVKMRLQ EPQACKHFTS FRHNSQLCVG NPKKMQNVYK
210 220 230 240
GDSGGPLLCA GIAQGIASYV HRNAKPPAVF TRISHYRPWI NKILREN
Length:247
Mass (Da):27,586
Last modified:December 1, 1992 - v2
Checksum:i24C290CF61237DC7
GO

Sequence cautioni

The sequence CAA48705 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5T → A in AAA40105 (PubMed:1939089).Curated1
Sequence conflicti51C → R AA sequence (PubMed:2326280).Curated1
Sequence conflicti224A → R in AAA39492 (PubMed:1376147).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73759 mRNA Translation: AAA40105.1
M73760 mRNA No translation available.
X68805 mRNA Translation: CAA48705.1 Different initiation.
M68898 mRNA Translation: AAA39492.1
AF119364 Genomic DNA Translation: AAD43901.1
CCDSiCCDS27135.1
PIRiS23504
S26043
RefSeqiNP_034910.1, NM_010780.3
UniGeneiMm.1252

Genome annotation databases

EnsembliENSMUST00000226280; ENSMUSP00000154406; ENSMUSG00000022225
GeneIDi17228
KEGGimmu:17228

Similar proteinsi

Entry informationi

Entry nameiCMA1_MOUSE
AccessioniPrimary (citable) accession number: P21844
Secondary accession number(s): Q9R1F0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: December 1, 1992
Last modified: June 20, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health