P21844 (CMA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chymase
EC=3.4.21.39

Alternative name(s):
Alpha-chymase
Mast cell chymase 1
Mast cell protease 5
Short name=mMCP-5
Mast cell protease I

Gene names

Name:	Cma1
Synonyms:	Mcpt5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Catalytic activity	Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.
Subcellular location	Secreted. Cytoplasmic granule. Note: Secretory granules. Ref.6
Tissue specificity	Mast cells.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.
Sequence caution	The sequence CAA48705.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 21	2	Activation peptide		PRO_0000027455
	Chain	22 – 247	226	Chymase		PRO_0000027456
Regions
	Domain	22 – 245	224	Peptidase S1
Sites
	Active site	66	1	Charge relay system By similarity
	Active site	110	1	Charge relay system By similarity
	Active site	203	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	144 ↔ 209		By similarity
	Disulfide bond	175 ↔ 188		By similarity
Experimental info
	Sequence conflict	5	1	T → A in AAA40105. Ref.1
	Sequence conflict	51	1	C → R AA sequence Ref.5
	Sequence conflict	224	1	A → R in AAA39492. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P21844 [UniParc]. Last modified December 1, 1992. Version 2. Checksum: 24C290CF61237DC7	FASTA	247	27,586
	10 20 30 40 50 60 MHLLTLHLLL LLLGSSTKAG EIIGGTECIP HSRPYMAYLE IVTSENYLSA CSGFLIRRNF 70 80 90 100 110 120 VLTAAHCAGR SITVLLGAHN KTSKEDTWQK LEVEKQFLHP KYDENLVVHD IMLLKLKEKA 130 140 150 160 170 180 KLTLGVGTLP LSANFNFIPP GRMCRAVGWG RTNVNEPASD TLQEVKMRLQ EPQACKHFTS 190 200 210 220 230 240 FRHNSQLCVG NPKKMQNVYK GDSGGPLLCA GIAQGIASYV HRNAKPPAVF TRISHYRPWI NKILREN

References

[1]	"Molecular cloning of the mouse mast cell protease-5 gene. A novel secretory granule protease expressed early in the differentiation of serosal mast cells." McNeil H.P., Austen K.F., Somerville L.L., Gurish M.F., Stevens R.L. J. Biol. Chem. 266:20316-20322(1991) [PubMed: 1939089] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases." Huang R., Blom T., Hellman L. Eur. J. Immunol. 21:1611-1621(1991) [PubMed: 2060576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Leaden X A1. Tissue: Mastocytoma.
[3]	"Molecular cloning and characterization of mouse mast cell chymases." Chu W., Johnson D.A., Musich P.R. Biochim. Biophys. Acta 1121:83-87(1992) [PubMed: 1376147] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Characterization of the gene encoding mouse mast cell protease 8 (mMCP-8), and a comparative analysis of hematopoietic serine protease genes." Lunderius C., Hellman L. Immunogenetics 53:225-232(2001) [PubMed: 11398967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19. Strain: 129/Sv.
[5]	"Different mouse mast cell populations express various combinations of at least six distinct mast cell serine proteases." Reynolds D.S., Stevens R.L., Lane W.S., Carr M.H., Austen K.F., Serafin W.E. Proc. Natl. Acad. Sci. U.S.A. 87:3230-3234(1990) [PubMed: 2326280] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-51.
[6]	"Translation and granule localization of mouse mast cell protease-5. Immunodetection with specific antipeptide Ig." McNeil H.P., Frenkel D.P., Austen F., Friend D.S., Stevens R.L. J. Immunol. 149:2466-2472(1992) [PubMed: 1527387] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73759 mRNA. Translation: AAA40105.1. M73760 mRNA. No translation available. X68805 mRNA. Translation: CAA48705.1. Different initiation. M68898 mRNA. Translation: AAA39492.1. AF119364 Genomic DNA. Translation: AAD43901.1.
IPI	IPI00230426.
PIR	S23504. S26043.
RefSeq	NP_034910.1. NM_010780.2.
UniGene	Mm.1252.
3D structure databases
ProteinModelPortal	P21844.
SMR	P21844. Positions 22-247.
ModBase	Search...
Protein-protein interaction databases
STRING	P21844.
Protein family/group databases
MEROPS	S01.150.
PTM databases
PhosphoSite	P21844.
Proteomic databases
PRIDE	P21844.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	17228.
KEGG	mmu:17228.
Organism-specific databases
CTD	1215.
MGI	MGI:96941. Cma1.
Phylogenomic databases
GeneTree	ENSGT00580000081281.
HOVERGEN	HBG013304.
InParanoid	P21844.
OrthoDB	EOG4R23VK.
Gene expression databases
ArrayExpress	P21844.
Bgee	P21844.
CleanEx	MM_CMA1.
Genevestigator	P21844.
GermOnline	ENSMUSG00000022225. Mus musculus.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
KO	K01329.
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291648.
SOURCE	Search...

Entry information

Entry name

CMA1_MOUSE

Accession

Primary (citable) accession number: P21844
Secondary accession number(s): Q9R1F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	December 1, 1992
Last modified:	November 16, 2011
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program