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P21844 (CMA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chymase

EC=3.4.21.39
Alternative name(s):
Alpha-chymase
Mast cell chymase 1
Mast cell protease 5
Short name=mMCP-5
Mast cell protease I
Gene names
Name:Cma1
Synonyms:Mcpt5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

Catalytic activity

Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

Subcellular location

Secreted. Cytoplasmic granule. Note: Secretory granules. Ref.6

Tissue specificity

Mast cells.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

Sequence caution

The sequence CAA48705.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide20 – 212Activation peptide
PRO_0000027455
Chain22 – 247226Chymase
PRO_0000027456

Regions

Domain22 – 245224Peptidase S1

Sites

Active site661Charge relay system By similarity
Active site1101Charge relay system By similarity
Active site2031Charge relay system By similarity

Amino acid modifications

Glycosylation801N-linked (GlcNAc...) Potential
Disulfide bond51 ↔ 67 By similarity
Disulfide bond144 ↔ 209 By similarity
Disulfide bond175 ↔ 188 By similarity

Experimental info

Sequence conflict51T → A in AAA40105. Ref.1
Sequence conflict511C → R AA sequence Ref.5
Sequence conflict2241A → R in AAA39492. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P21844 [UniParc].

Last modified December 1, 1992. Version 2.
Checksum: 24C290CF61237DC7

FASTA24727,586
        10         20         30         40         50         60 
MHLLTLHLLL LLLGSSTKAG EIIGGTECIP HSRPYMAYLE IVTSENYLSA CSGFLIRRNF 

        70         80         90        100        110        120 
VLTAAHCAGR SITVLLGAHN KTSKEDTWQK LEVEKQFLHP KYDENLVVHD IMLLKLKEKA 

       130        140        150        160        170        180 
KLTLGVGTLP LSANFNFIPP GRMCRAVGWG RTNVNEPASD TLQEVKMRLQ EPQACKHFTS 

       190        200        210        220        230        240 
FRHNSQLCVG NPKKMQNVYK GDSGGPLLCA GIAQGIASYV HRNAKPPAVF TRISHYRPWI 


NKILREN 

« Hide

References

[1]"Molecular cloning of the mouse mast cell protease-5 gene. A novel secretory granule protease expressed early in the differentiation of serosal mast cells."
McNeil H.P., Austen K.F., Somerville L.L., Gurish M.F., Stevens R.L.
J. Biol. Chem. 266:20316-20322(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases."
Huang R., Blom T., Hellman L.
Eur. J. Immunol. 21:1611-1621(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Leaden X A1.
Tissue: Mastocytoma.
[3]"Molecular cloning and characterization of mouse mast cell chymases."
Chu W., Johnson D.A., Musich P.R.
Biochim. Biophys. Acta 1121:83-87(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Characterization of the gene encoding mouse mast cell protease 8 (mMCP-8), and a comparative analysis of hematopoietic serine protease genes."
Lunderius C., Hellman L.
Immunogenetics 53:225-232(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
Strain: 129/Sv.
[5]"Different mouse mast cell populations express various combinations of at least six distinct mast cell serine proteases."
Reynolds D.S., Stevens R.L., Lane W.S., Carr M.H., Austen K.F., Serafin W.E.
Proc. Natl. Acad. Sci. U.S.A. 87:3230-3234(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 22-51.
[6]"Translation and granule localization of mouse mast cell protease-5. Immunodetection with specific antipeptide Ig."
McNeil H.P., Frenkel D.P., Austen F., Friend D.S., Stevens R.L.
J. Immunol. 149:2466-2472(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M73759 mRNA. Translation: AAA40105.1.
M73760 mRNA. No translation available.
X68805 mRNA. Translation: CAA48705.1. Different initiation.
M68898 mRNA. Translation: AAA39492.1.
AF119364 Genomic DNA. Translation: AAD43901.1.
PIRS23504.
S26043.
RefSeqNP_034910.1. NM_010780.2.
UniGeneMm.1252.

3D structure databases

ProteinModelPortalP21844.
SMRP21844. Positions 22-247.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.150.

PTM databases

PhosphoSiteP21844.

Proteomic databases

PaxDbP21844.
PRIDEP21844.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID17228.
KEGGmmu:17228.

Organism-specific databases

CTD1215.
MGIMGI:96941. Cma1.

Phylogenomic databases

eggNOGNOG293191.
HOVERGENHBG013304.
InParanoidP21844.
KOK01329.

Gene expression databases

ArrayExpressP21844.
BgeeP21844.
CleanExMM_CMA1.
GenevestigatorP21844.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291648.
PROP21844.
SOURCESearch...

Entry information

Entry nameCMA1_MOUSE
AccessionPrimary (citable) accession number: P21844
Secondary accession number(s): Q9R1F0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot