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P21844

- CMA1_MOUSE

UniProt

P21844 - CMA1_MOUSE

Protein

Chymase

Gene

Cma1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

    Catalytic activityi

    Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei66 – 661Charge relay systemBy similarity
    Active sitei110 – 1101Charge relay systemBy similarity
    Active sitei203 – 2031Charge relay systemBy similarity

    GO - Molecular functioni

    1. peptide binding Source: Ensembl
    2. serine-type endopeptidase activity Source: RefGenome

    GO - Biological processi

    1. cellular response to glucose stimulus Source: Ensembl
    2. interleukin-1 beta biosynthetic process Source: Ensembl
    3. midbrain development Source: Ensembl
    4. peptide metabolic process Source: Ensembl
    5. positive regulation of angiogenesis Source: Ensembl
    6. proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    ReactomeiREACT_188578. Signaling by SCF-KIT.
    REACT_196539. Metabolism of Angiotensinogen to Angiotensins.
    REACT_199000. Activation of Matrix Metalloproteinases.

    Protein family/group databases

    MEROPSiS01.150.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chymase (EC:3.4.21.39)
    Alternative name(s):
    Alpha-chymase
    Mast cell chymase 1
    Mast cell protease 5
    Short name:
    mMCP-5
    Mast cell protease I
    Gene namesi
    Name:Cma1
    Synonyms:Mcpt5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:96941. Cma1.

    Subcellular locationi

    Secreted 1 Publication. Cytoplasmic granule 1 Publication
    Note: Secretory granules.

    GO - Cellular componenti

    1. extracellular matrix Source: Ensembl
    2. extracellular region Source: UniProtKB-SubCell
    3. intracellular Source: MGI
    4. secretory granule Source: RefGenome

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Propeptidei20 – 212Activation peptide1 PublicationPRO_0000027455
    Chaini22 – 247226ChymasePRO_0000027456Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi51 ↔ 67PROSITE-ProRule annotation
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi144 ↔ 209PROSITE-ProRule annotation
    Disulfide bondi175 ↔ 188PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP21844.
    PRIDEiP21844.

    PTM databases

    PhosphoSiteiP21844.

    Expressioni

    Tissue specificityi

    Mast cells.

    Gene expression databases

    ArrayExpressiP21844.
    BgeeiP21844.
    CleanExiMM_CMA1.
    GenevestigatoriP21844.

    Structurei

    3D structure databases

    ProteinModelPortaliP21844.
    SMRiP21844. Positions 22-247.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 245224Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG293191.
    HOVERGENiHBG013304.
    InParanoidiP21844.
    KOiK01329.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21844-1 [UniParc]FASTAAdd to Basket

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    MHLLTLHLLL LLLGSSTKAG EIIGGTECIP HSRPYMAYLE IVTSENYLSA    50
    CSGFLIRRNF VLTAAHCAGR SITVLLGAHN KTSKEDTWQK LEVEKQFLHP 100
    KYDENLVVHD IMLLKLKEKA KLTLGVGTLP LSANFNFIPP GRMCRAVGWG 150
    RTNVNEPASD TLQEVKMRLQ EPQACKHFTS FRHNSQLCVG NPKKMQNVYK 200
    GDSGGPLLCA GIAQGIASYV HRNAKPPAVF TRISHYRPWI NKILREN 247
    Length:247
    Mass (Da):27,586
    Last modified:December 1, 1992 - v2
    Checksum:i24C290CF61237DC7
    GO

    Sequence cautioni

    The sequence CAA48705.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51T → A in AAA40105. (PubMed:1939089)Curated
    Sequence conflicti51 – 511C → R AA sequence (PubMed:2326280)Curated
    Sequence conflicti224 – 2241A → R in AAA39492. (PubMed:1376147)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73759 mRNA. Translation: AAA40105.1.
    M73760 mRNA. No translation available.
    X68805 mRNA. Translation: CAA48705.1. Different initiation.
    M68898 mRNA. Translation: AAA39492.1.
    AF119364 Genomic DNA. Translation: AAD43901.1.
    PIRiS23504.
    S26043.
    RefSeqiNP_034910.1. NM_010780.2.
    UniGeneiMm.1252.

    Genome annotation databases

    GeneIDi17228.
    KEGGimmu:17228.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73759 mRNA. Translation: AAA40105.1 .
    M73760 mRNA. No translation available.
    X68805 mRNA. Translation: CAA48705.1 . Different initiation.
    M68898 mRNA. Translation: AAA39492.1 .
    AF119364 Genomic DNA. Translation: AAD43901.1 .
    PIRi S23504.
    S26043.
    RefSeqi NP_034910.1. NM_010780.2.
    UniGenei Mm.1252.

    3D structure databases

    ProteinModelPortali P21844.
    SMRi P21844. Positions 22-247.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.150.

    PTM databases

    PhosphoSitei P21844.

    Proteomic databases

    PaxDbi P21844.
    PRIDEi P21844.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 17228.
    KEGGi mmu:17228.

    Organism-specific databases

    CTDi 1215.
    MGIi MGI:96941. Cma1.

    Phylogenomic databases

    eggNOGi NOG293191.
    HOVERGENi HBG013304.
    InParanoidi P21844.
    KOi K01329.

    Enzyme and pathway databases

    Reactomei REACT_188578. Signaling by SCF-KIT.
    REACT_196539. Metabolism of Angiotensinogen to Angiotensins.
    REACT_199000. Activation of Matrix Metalloproteinases.

    Miscellaneous databases

    NextBioi 291648.
    PROi P21844.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P21844.
    Bgeei P21844.
    CleanExi MM_CMA1.
    Genevestigatori P21844.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the mouse mast cell protease-5 gene. A novel secretory granule protease expressed early in the differentiation of serosal mast cells."
      McNeil H.P., Austen K.F., Somerville L.L., Gurish M.F., Stevens R.L.
      J. Biol. Chem. 266:20316-20322(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases."
      Huang R., Blom T., Hellman L.
      Eur. J. Immunol. 21:1611-1621(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Leaden X A1.
      Tissue: Mastocytoma.
    3. "Molecular cloning and characterization of mouse mast cell chymases."
      Chu W., Johnson D.A., Musich P.R.
      Biochim. Biophys. Acta 1121:83-87(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Characterization of the gene encoding mouse mast cell protease 8 (mMCP-8), and a comparative analysis of hematopoietic serine protease genes."
      Lunderius C., Hellman L.
      Immunogenetics 53:225-232(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
      Strain: 129/Sv.
    5. "Different mouse mast cell populations express various combinations of at least six distinct mast cell serine proteases."
      Reynolds D.S., Stevens R.L., Lane W.S., Carr M.H., Austen K.F., Serafin W.E.
      Proc. Natl. Acad. Sci. U.S.A. 87:3230-3234(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 22-51.
    6. "Translation and granule localization of mouse mast cell protease-5. Immunodetection with specific antipeptide Ig."
      McNeil H.P., Frenkel D.P., Austen F., Friend D.S., Stevens R.L.
      J. Immunol. 149:2466-2472(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCMA1_MOUSE
    AccessioniPrimary (citable) accession number: P21844
    Secondary accession number(s): Q9R1F0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3