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P21844

- CMA1_MOUSE

UniProt

P21844 - CMA1_MOUSE

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Protein
Chymase
Gene
Cma1, Mcpt5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

Catalytic activityi

Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Charge relay system By similarity
Active sitei110 – 1101Charge relay system By similarity
Active sitei203 – 2031Charge relay system By similarity

GO - Molecular functioni

  1. peptide binding Source: Ensembl
  2. serine-type endopeptidase activity Source: RefGenome

GO - Biological processi

  1. cellular response to glucose stimulus Source: Ensembl
  2. interleukin-1 beta biosynthetic process Source: Ensembl
  3. midbrain development Source: Ensembl
  4. peptide metabolic process Source: Ensembl
  5. positive regulation of angiogenesis Source: Ensembl
  6. proteolysis Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_188578. Signaling by SCF-KIT.
REACT_196539. Metabolism of Angiotensinogen to Angiotensins.
REACT_199000. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiS01.150.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymase (EC:3.4.21.39)
Alternative name(s):
Alpha-chymase
Mast cell chymase 1
Mast cell protease 5
Short name:
mMCP-5
Mast cell protease I
Gene namesi
Name:Cma1
Synonyms:Mcpt5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:96941. Cma1.

Subcellular locationi

Secreted. Cytoplasmic granule
Note: Secretory granules.1 Publication

GO - Cellular componenti

  1. extracellular matrix Source: Ensembl
  2. extracellular region Source: UniProtKB-SubCell
  3. intracellular Source: MGI
  4. secretory granule Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Propeptidei20 – 212Activation peptide
PRO_0000027455
Chaini22 – 247226Chymase
PRO_0000027456Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi51 ↔ 67 By similarity
Glycosylationi80 – 801N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi144 ↔ 209 By similarity
Disulfide bondi175 ↔ 188 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP21844.
PRIDEiP21844.

PTM databases

PhosphoSiteiP21844.

Expressioni

Tissue specificityi

Mast cells.

Gene expression databases

ArrayExpressiP21844.
BgeeiP21844.
CleanExiMM_CMA1.
GenevestigatoriP21844.

Structurei

3D structure databases

ProteinModelPortaliP21844.
SMRiP21844. Positions 22-247.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 245224Peptidase S1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG293191.
HOVERGENiHBG013304.
InParanoidiP21844.
KOiK01329.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21844-1 [UniParc]FASTAAdd to Basket

« Hide

MHLLTLHLLL LLLGSSTKAG EIIGGTECIP HSRPYMAYLE IVTSENYLSA    50
CSGFLIRRNF VLTAAHCAGR SITVLLGAHN KTSKEDTWQK LEVEKQFLHP 100
KYDENLVVHD IMLLKLKEKA KLTLGVGTLP LSANFNFIPP GRMCRAVGWG 150
RTNVNEPASD TLQEVKMRLQ EPQACKHFTS FRHNSQLCVG NPKKMQNVYK 200
GDSGGPLLCA GIAQGIASYV HRNAKPPAVF TRISHYRPWI NKILREN 247
Length:247
Mass (Da):27,586
Last modified:December 1, 1992 - v2
Checksum:i24C290CF61237DC7
GO

Sequence cautioni

The sequence CAA48705.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51T → A in AAA40105. 1 Publication
Sequence conflicti51 – 511C → R AA sequence 1 Publication
Sequence conflicti224 – 2241A → R in AAA39492. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73759 mRNA. Translation: AAA40105.1.
M73760 mRNA. No translation available.
X68805 mRNA. Translation: CAA48705.1. Different initiation.
M68898 mRNA. Translation: AAA39492.1.
AF119364 Genomic DNA. Translation: AAD43901.1.
PIRiS23504.
S26043.
RefSeqiNP_034910.1. NM_010780.2.
UniGeneiMm.1252.

Genome annotation databases

GeneIDi17228.
KEGGimmu:17228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73759 mRNA. Translation: AAA40105.1 .
M73760 mRNA. No translation available.
X68805 mRNA. Translation: CAA48705.1 . Different initiation.
M68898 mRNA. Translation: AAA39492.1 .
AF119364 Genomic DNA. Translation: AAD43901.1 .
PIRi S23504.
S26043.
RefSeqi NP_034910.1. NM_010780.2.
UniGenei Mm.1252.

3D structure databases

ProteinModelPortali P21844.
SMRi P21844. Positions 22-247.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.150.

PTM databases

PhosphoSitei P21844.

Proteomic databases

PaxDbi P21844.
PRIDEi P21844.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 17228.
KEGGi mmu:17228.

Organism-specific databases

CTDi 1215.
MGIi MGI:96941. Cma1.

Phylogenomic databases

eggNOGi NOG293191.
HOVERGENi HBG013304.
InParanoidi P21844.
KOi K01329.

Enzyme and pathway databases

Reactomei REACT_188578. Signaling by SCF-KIT.
REACT_196539. Metabolism of Angiotensinogen to Angiotensins.
REACT_199000. Activation of Matrix Metalloproteinases.

Miscellaneous databases

NextBioi 291648.
PROi P21844.
SOURCEi Search...

Gene expression databases

ArrayExpressi P21844.
Bgeei P21844.
CleanExi MM_CMA1.
Genevestigatori P21844.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the mouse mast cell protease-5 gene. A novel secretory granule protease expressed early in the differentiation of serosal mast cells."
    McNeil H.P., Austen K.F., Somerville L.L., Gurish M.F., Stevens R.L.
    J. Biol. Chem. 266:20316-20322(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases."
    Huang R., Blom T., Hellman L.
    Eur. J. Immunol. 21:1611-1621(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Leaden X A1.
    Tissue: Mastocytoma.
  3. "Molecular cloning and characterization of mouse mast cell chymases."
    Chu W., Johnson D.A., Musich P.R.
    Biochim. Biophys. Acta 1121:83-87(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Characterization of the gene encoding mouse mast cell protease 8 (mMCP-8), and a comparative analysis of hematopoietic serine protease genes."
    Lunderius C., Hellman L.
    Immunogenetics 53:225-232(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
    Strain: 129/Sv.
  5. "Different mouse mast cell populations express various combinations of at least six distinct mast cell serine proteases."
    Reynolds D.S., Stevens R.L., Lane W.S., Carr M.H., Austen K.F., Serafin W.E.
    Proc. Natl. Acad. Sci. U.S.A. 87:3230-3234(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 22-51.
  6. "Translation and granule localization of mouse mast cell protease-5. Immunodetection with specific antipeptide Ig."
    McNeil H.P., Frenkel D.P., Austen F., Friend D.S., Stevens R.L.
    J. Immunol. 149:2466-2472(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCMA1_MOUSE
AccessioniPrimary (citable) accession number: P21844
Secondary accession number(s): Q9R1F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: December 1, 1992
Last modified: September 3, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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