Reviewed, UniProtKB/Swiss-Prot P21842 (CMA1_CANFA)

Names and origin

Protein names

Recommended name:
    Chymase
    EC=3.4.21.39
Alternative name(s):
    Alpha-chymase
    Mast cell protease I

Gene names

Name:

CMA1

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Catalytic activity	Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.
Subcellular location	Secreted By similarity. Cytoplasmic granule By similarity. Note: Mast cell granules By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19
	Propeptide	20 – 21	2	Activation peptide Ref.3		PRO_0000027431
	Chain	22 – 249	228	Chymase		PRO_0000027432
Regions
	Domain	22 – 245	224	Peptidase S1
Sites
	Active site	66	1	Charge relay system By similarity
	Active site	110	1	Charge relay system By similarity
	Active site	203	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	51 ↔ 67		By similarity
	Disulfide bond	144 ↔ 209		By similarity
	Disulfide bond	175 ↔ 188		By similarity
Experimental info
	Sequence conflict	29	1	K → R AA sequence Ref.3
	Sequence conflict	38	1	H → Q AA sequence Ref.3
	Sequence conflict	45	1	R → T AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P21842-1 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 3BBD0A6C2855F540	FASTA	249	27,812
	10 20 30 40 50 60 MHCLPLTLLL LLLCSRAEAE EIIGGTESKP HSRPYMAHLE ILTLRNHLAS CGGFLIRRNF 70 80 90 100 110 120 VLTAAHCAGR FIMVTLGAHN IQKKEDTWQK LEVIKQFPHP KYDDLTLRHD IMLLKLKEKA 130 140 150 160 170 180 NLTLAVGTLP LSPQFNFVPP GRMCRVAGWG KRQVNGSGSD TLQEVKLRLM DPQACRHYMA 190 200 210 220 230 240 FDHNLQLCVG NPRKTKSAFK GDSGGPLLCA GVAQGIVSYG QNDAKPPAVF TRISHYRPWI NKVLKQNKA

References

[1]	"Dog mast cell chymase: molecular cloning and characterization." Caughey G.H., Raymond W.W., Vanderslice P. Biochemistry 29:5166-5171(1990) [PubMed: 2378872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and expression of the dog mast cell alpha-chymase gene." Caughey G.H., Blount J.L., Koerber K.L., Kitamura M., Fang K.C. J. Immunol. 159:4367-4375(1997) [PubMed: 9379034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Beagle.
[3]	"Purification and characterization of dog mastocytoma chymase: identification of an octapeptide conserved in chymotryptic leukocyte proteinases." Caughey G.H., Viro N.F., Lazarus S.C., Nadel J.A. Biochim. Biophys. Acta 952:142-149(1988) [PubMed: 3122835] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-46.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02904 mRNA. Translation: AAA30835.1. U89607 Genomic DNA. Translation: AAB94641.1.
PIR	A35842.
RefSeq	NP_001013442.1. XP_855610.1.
UniGene	Cfa.16336
3D structure databases
HSSP	HSSP built from PDB template 1NN6 based on UniProtKB P23946.
SMR	P21842. Positions 22-247.
ModBase	Search...
Protein family/group databases
MEROPS	S01.140.
Genome annotation databases
Ensembl	ENSCAFG00000012443. Canis familiaris. [Contig view]
GeneID	490628.
KEGG	cfa:490628.
Phylogenomic databases
HOVERGEN	P21842.
Enzyme and pathway databases
BRENDA	3.4.21.39. 463.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMA1_CANFA

Accession

Primary (citable) accession number: P21842

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)