Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-oxoisovalerate dehydrogenase subunit beta, mitochondrial

Gene

BCKDHB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).

Catalytic activityi

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

SABIO-RKP21839.

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoisovalerate dehydrogenase subunit beta, mitochondrial (EC:1.2.4.4)
Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase E1 component beta chain
Short name:
BCKDE1B
Short name:
BCKDH E1-beta
Gene namesi
Name:BCKDHB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50MitochondrionAdd BLAST50
ChainiPRO_000002046951 – 3922-oxoisovalerate dehydrogenase subunit beta, mitochondrialAdd BLAST342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei232N6-acetyllysineBy similarity1
Modified residuei241N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP21839.

Interactioni

Subunit structurei

Heterotetramer of 2 alpha and 2 beta chains.By similarity

Structurei

3D structure databases

ProteinModelPortaliP21839.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000281451.
HOVERGENiHBG108210.
InParanoidiP21839.
KOiK00167.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21839-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVAAFAGW LLRLRAAGAD GPWRRLCGAG LSRGFLQSAS AYGAAAQRRQ
60 70 80 90 100
VAHFTFQPDP EPVEYGQTQK MNLFQAVTSA LDNSLAKDPT AVIFGEDVAF
110 120 130 140 150
GGVFRCTVGL RDKYGKDRVF NTPLCEQGIV GFGIGIAVTG ATAIAEIQFA
160 170 180 190 200
DYIFPAFDQI VNEAAKYRYR SGDLFNCGSL TIRSPWGCVG HGALYHSQSP
210 220 230 240 250
EAFFAHCPGI KVVVPRSPFQ AKGLLLSCIE DKNPCIFFEP KILYRAAVEQ
260 270 280 290 300
VPVEPYNIPL SQAEVIQEGS DVTLVAWGTQ VHVIREVAAM AQEKLGVSCE
310 320 330 340 350
VIDLRTILPW DVDTVCKSVI KTGRLLVSHE APLTGGFASE ISSTVQEECF
360 370 380 390
LNLEAPISRV CGYDTPFPHI FEPFYIPDKW KCYDALRKMI NY
Length:392
Mass (Da):42,935
Last modified:October 3, 2006 - v2
Checksum:i4E3ABC39ED54281A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24 – 46RRLCG…YGAAA → GPGCVARACRGASCSPPRPT GLR (PubMed:1730724).CuratedAdd BLAST23
Sequence conflicti28G → C in AAI18381 (Ref. 2) Curated1
Sequence conflicti283V → E in AAA51410 (PubMed:1730724).Curated1
Sequence conflicti288A → D in AAA30407 (PubMed:2322554).Curated1
Sequence conflicti348E → Q in AAA51410 (PubMed:1730724).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33323 mRNA. Translation: AAA30407.1.
BC118380 mRNA. Translation: AAI18381.1.
M81742 mRNA. Translation: AAA51410.1.
PIRiA34267.
RefSeqiNP_776932.1. NM_174507.2.
UniGeneiBt.5412.

Genome annotation databases

GeneIDi282150.
KEGGibta:282150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33323 mRNA. Translation: AAA30407.1.
BC118380 mRNA. Translation: AAI18381.1.
M81742 mRNA. Translation: AAA51410.1.
PIRiA34267.
RefSeqiNP_776932.1. NM_174507.2.
UniGeneiBt.5412.

3D structure databases

ProteinModelPortaliP21839.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP21839.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282150.
KEGGibta:282150.

Organism-specific databases

CTDi594.

Phylogenomic databases

HOGENOMiHOG000281451.
HOVERGENiHBG108210.
InParanoidiP21839.
KOiK00167.

Enzyme and pathway databases

SABIO-RKP21839.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR029061. THDP-binding.
IPR009014. Transketo_C/PFOR_II.
IPR005475. Transketolase-like_Pyr-bd.
IPR033248. Transketolase_C.
IPR033247. Transketolase_fam.
[Graphical view]
PANTHERiPTHR11624. PTHR11624. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiODBB_BOVIN
AccessioniPrimary (citable) accession number: P21839
Secondary accession number(s): Q148F4, Q28047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: October 3, 2006
Last modified: October 5, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.