Reviewed, UniProtKB/Swiss-Prot P21839 (ODBB_BOVIN)

Names and origin

Protein names

Recommended name:
    2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
    EC=1.2.4.4
Alternative name(s):
    Branched-chain alpha-keto acid dehydrogenase E1 component beta chain
      Short name=BCKDH E1-beta

Gene names

Name:

BCKDHB

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Catalytic activity	3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.
Subunit structure	Heterodimer of an alpha and a beta chain.
Subcellular location	Mitochondrion matrix.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 50	50	Mitochondrion
	Chain	51 – 392	342	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial		PRO_0000020469
Amino acid modifications
	Modified residue	241	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	24 – 46	23	RRLCG…YGAAA → GPGCVARACRGASCSPPRPT GLR Ref.3
	Sequence conflict	28	1	G → C in AAI18381. Ref.2
	Sequence conflict	283	1	V → E in AAA51410. Ref.3
	Sequence conflict	288	1	A → D in AAA30407. Ref.1
	Sequence conflict	348	1	E → Q in AAA51410. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P21839-1 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: 4E3ABC39ED54281A	FASTA	392	42,935
	10 20 30 40 50 60 MAAVAAFAGW LLRLRAAGAD GPWRRLCGAG LSRGFLQSAS AYGAAAQRRQ VAHFTFQPDP 70 80 90 100 110 120 EPVEYGQTQK MNLFQAVTSA LDNSLAKDPT AVIFGEDVAF GGVFRCTVGL RDKYGKDRVF 130 140 150 160 170 180 NTPLCEQGIV GFGIGIAVTG ATAIAEIQFA DYIFPAFDQI VNEAAKYRYR SGDLFNCGSL 190 200 210 220 230 240 TIRSPWGCVG HGALYHSQSP EAFFAHCPGI KVVVPRSPFQ AKGLLLSCIE DKNPCIFFEP 250 260 270 280 290 300 KILYRAAVEQ VPVEPYNIPL SQAEVIQEGS DVTLVAWGTQ VHVIREVAAM AQEKLGVSCE 310 320 330 340 350 360 VIDLRTILPW DVDTVCKSVI KTGRLLVSHE APLTGGFASE ISSTVQEECF LNLEAPISRV 370 380 390 CGYDTPFPHI FEPFYIPDKW KCYDALRKMI NY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a complementary DNA clone coding for the E1 beta subunit of the bovine branched-chain alpha-ketoacid dehydrogenase complex: complete amino acid sequence of the precursor protein and its proteolytic processing." Nobukuni Y., Mitsubuchi H., Endo F., Asaka J., Oyama R., Titani K., Matsuda I. Biochemistry 29:1154-1160(1990) [PubMed: 2322554] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal pons.
[3]	"Cloning and expression in Escherichia coli of mature E1 beta subunit of bovine mitochondrial branched-chain alpha-keto acid dehydrogenase complex. Mapping of the E1 beta-binding region on E2." Wynn R.M., Chuang J.L., Davie J.R., Fisher C.W., Hale M.A., Cox R.P., Chuang D.T. J. Biol. Chem. 267:1881-1887(1992) [PubMed: 1730724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-392. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M33323 mRNA. Translation: AAA30407.1. BC118380 mRNA. Translation: AAI18381.1. M81742 mRNA. Translation: AAA51410.1.
IPI	IPI00696901.
PIR	A34267.
RefSeq	NP_776932.1.
UniGene	Bt.5412
3D structure databases
HSSP	HSSP built from PDB template 1DTW based on UniProtKB P21953.
SMR	P21839. Positions 64-392.
ModBase	Search...
Protein-protein interaction databases
STRING	P21839.
Genome annotation databases
Ensembl	ENSBTAT00000016044; ENSBTAP00000016044; ENSBTAG00000012096; Bos taurus. [Genome view]
GeneID	282150.
KEGG	bta:282150.
Organism-specific databases
CTD	282150.
Phylogenomic databases
HOVERGEN	P21839.
OMA	HEATLTS.
Enzyme and pathway databases
BRENDA	1.2.4.4. 251.
Family and domain databases
InterPro	IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ODBB_BOVIN

Accession

Primary (citable) accession number: P21839
Secondary accession number(s): Q148F4, Q28047

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	October 3, 2006
Last modified:	November 3, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)