P21829 (YBHA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatase YbhA
EC=3.1.3.-

Gene names

Name:	ybhA
Ordered Locus Names:	b0766, JW0749

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates pyridoxalphosphate and erythrose 4-phosphate. Ref.6
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. Cof family.
Caution	This ORF is coded on the other strand of an ORF which has been called modD (by Ref.1 and Ref.2), but which seems to be wrong.
Sequence caution	The sequence U07867 differs from that shown. Reason: Frameshift at position 217.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	magnesium ion binding Inferred from direct assay. Source: EcoliWiki phosphotransferase activity, alcohol group as acceptor Inferred from direct assay. Source: EcoliWiki pyridoxal phosphatase activity Inferred from direct assay. Source: EcoliWiki sugar-phosphatase activity Inferred from direct assay. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Phosphatase YbhA		PRO_0000054420
Sites
	Active site	9	1	Nucleophile By similarity
	Metal binding	9	1	Magnesium By similarity
	Metal binding	11	1	Magnesium By similarity
	Metal binding	223	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P21829 [UniParc]. Last modified October 1, 1996. Version 3. Checksum: BB4602AB4D26ACD2	FASTA	272	30,201
	10 20 30 40 50 60 MTTRVIALDL DGTLLTPKKT LLPSSIEALA RAREAGYQLI IVTGRHHVAI HPFYQALALD 70 80 90 100 110 120 TPAICCNGTY LYDYHAKTVL EADPMPVIKA LQLIEMLNEH HIHGLMYVDD AMVYEHPTGH 130 140 150 160 170 180 VIRTSNWAQT LPPEQRPTFT QVASLAETAQ QVNAVWKFAL THDDLPQLQH FGKHVEHELG 190 200 210 220 230 240 LECEWSWHDQ VDIARGGNSK GKRLTKWVEA QGWSMENVVA FGDNFNDISM LEAAGTGVAM 250 260 270 GNADDAVKAR ANIVIGDNTT DSIAQFIYSH LI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli." Maupin-Furlow J.A., Rosentel J.K., Lee J.H., Deppenmeier U., Gunsalus R.P., Shanmugam K.T. J. Bacteriol. 177:4851-4856(1995) [PubMed: 7665460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR, a regulatory gene." Walkenhorst H.M., Hemschemeier S.K., Eichenlaub R. Microbiol. Res. 150:347-361(1995) [PubMed: 8564363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MC1000 / ATCC 39531.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Enzyme genomics: application of general enzymatic screens to discover new enzymes." Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A., Arrowsmith C.H., Edwards A.M., Yakunin A.F. FEMS Microbiol. Rev. 29:263-279(2005) [PubMed: 15808744] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U27192 Genomic DNA. Translation: AAB60177.1. U07867 Genomic DNA. No translation available. U00096 Genomic DNA. Translation: AAC73853.1. AP009048 Genomic DNA. Translation: BAA35430.1.
PIR	F64812.
RefSeq	NP_415287.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P21829.
SMR	P21829. Positions 2-271.
ModBase	Search...
Protein-protein interaction databases
IntAct	P21829. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002252; EBESCP00000002252; EBESCG00000001847. EBESCT00000016878; EBESCP00000016169; EBESCG00000015937.
GeneID	945372.
GenomeReviews	Gene locus JW0749 in contig AP009048_GR. Gene locus b0766 in contig U00096_GR.
KEGG	ecj:JW0749. eco:b0766.
PATRIC	32116733. VBIEscCol129921_0792.
Organism-specific databases
EchoBASE	EB1221.
EcoGene	EG11239. ybhA.
Phylogenomic databases
eggNOG	COG0561.
GeneTree	EBGT00050000009623.
HOGENOM	HBG728500.
OMA	TLGLECE.
PhylomeDB	P21829.
ProtClustDB	PRK10530.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11239-MONOMER. MetaCyc:EG11239-MONOMER.
Gene expression databases
Genevestigator	P21829.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. IPR006379. HAD-SF_hydro_IIB. IPR000150. Hypothet_cof. IPR006380. Sucrose-P_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K07024.
Pfam	PF00702. Hydrolase. 1 hit. PF05116. S6PP. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR00099. Cof-subfamily. 1 hit. TIGR01484. HAD-SF-IIB. 1 hit.
PROSITE	PS01228. COF_1. 1 hit. PS01229. COF_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YBHA_ECOLI

Accession

Primary (citable) accession number: P21829

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 99 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program