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Protein

Guanine nucleotide exchange factor SRM1

Gene

SRM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor that promotes the exchange of GSP1/GSP2-bound GDP by GTP and controls RNA metabolism and transport. Involved in yeast pheromone response pathway and in mRNA metabolism. Involved in nuclear pore complex (NPC) assembly and required for mRNA and ribosome nuclear export. Binds chromatin and is involved NPC-mediated transcriptional control.14 Publications

GO - Molecular functioni

  • Ran guanyl-nucleotide exchange factor activity Source: SGD
  • signal transducer activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • response to pheromone Source: UniProtKB-KW
  • ribosomal subunit export from nucleus Source: SGD
  • rRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Pheromone response, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30597-MONOMER.
ReactomeiR-SCE-3108214. SUMOylation of DNA damage response and repair proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor SRM1
Alternative name(s):
Pheromone response pathway component SRM1
Pre-mRNA-processing protein 20
Regulator of chromosome condensation
Suppressor of receptor mutations 1
mRNA transport protein 1
Gene namesi
Name:SRM1
Synonyms:MTR1, PRP20
Ordered Locus Names:YGL097W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL097W.
SGDiS000003065. SRM1.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromatin Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 191K → T: Impairs correct nuclear localization; when associated with T-20 and T-23. 1 Publication
Mutagenesisi20 – 201K → A or Q: Impairs activity. 1 Publication
Mutagenesisi20 – 201K → T: Impairs correct nuclear localization; when associated with T-19 and T-23. 1 Publication
Mutagenesisi23 – 231K → T: Impairs correct nuclear localization; when associated with T-19 and T-20. 1 Publication
Mutagenesisi282 – 2821G → S: Leads to temperature-dependent mislocalization of nucleoporins (nups) and the pore-membrane protein POM152. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482Guanine nucleotide exchange factor SRM1PRO_0000206634Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei135 – 1351PhosphoserineCombined sources
Modified residuei136 – 1361PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated; possibly by KSP1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21827.

PTM databases

iPTMnetiP21827.

Expressioni

Inductioni

By pheromone.

Interactioni

Subunit structurei

Component of a multicomponent complex composed of six to seven proteins, which has a collective molecular mass greater than 150 kDa. Interacts with GSP1 and YRB2.4 Publications

Protein-protein interaction databases

BioGridi33153. 82 interactions.
DIPiDIP-2315N.
IntActiP21827. 3 interactions.
MINTiMINT-561451.

Structurei

Secondary structure

1
482
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi33 – 364Combined sources
Helixi37 – 404Combined sources
Beta strandi47 – 537Combined sources
Helixi65 – 684Combined sources
Beta strandi69 – 757Combined sources
Turni81 – 833Combined sources
Beta strandi86 – 916Combined sources
Beta strandi93 – 1008Combined sources
Beta strandi105 – 1095Combined sources
Helixi145 – 1484Combined sources
Helixi155 – 1573Combined sources
Helixi159 – 1624Combined sources
Beta strandi167 – 1726Combined sources
Beta strandi174 – 1818Combined sources
Beta strandi186 – 1905Combined sources
Beta strandi192 – 1943Combined sources
Beta strandi197 – 2026Combined sources
Turni203 – 2053Combined sources
Beta strandi209 – 2146Combined sources
Beta strandi223 – 2286Combined sources
Beta strandi230 – 2378Combined sources
Beta strandi242 – 2465Combined sources
Helixi259 – 2613Combined sources
Turni262 – 2654Combined sources
Beta strandi275 – 2817Combined sources
Beta strandi283 – 2908Combined sources
Beta strandi295 – 3006Combined sources
Beta strandi317 – 3237Combined sources
Beta strandi332 – 3376Combined sources
Beta strandi339 – 3468Combined sources
Beta strandi351 – 3566Combined sources
Helixi366 – 3683Combined sources
Beta strandi381 – 3899Combined sources
Beta strandi396 – 4016Combined sources
Beta strandi403 – 4108Combined sources
Beta strandi415 – 4195Combined sources
Beta strandi435 – 4406Combined sources
Turni444 – 4485Combined sources
Beta strandi449 – 4568Combined sources
Beta strandi458 – 46710Combined sources
Helixi470 – 48112Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OF7X-ray1.90A27-482[»]
4OIHX-ray2.10B1-25[»]
5HQ2X-ray4.50K2-482[»]
ProteinModelPortaliP21827.
SMRiP21827. Positions 27-482.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21827.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati45 – 10157RCC1 1Add
BLAST
Repeati103 – 15250RCC1 2Add
BLAST
Repeati183 – 23856RCC1 3Add
BLAST
Repeati239 – 29153RCC1 4Add
BLAST
Repeati292 – 34756RCC1 5Add
BLAST
Repeati349 – 41163RCC1 6Add
BLAST
Repeati412 – 46655RCC1 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi15 – 2612Nuclear localization signal1 PublicationAdd
BLAST

Sequence similaritiesi

Contains 7 RCC1 repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00840000129719.
HOGENOMiHOG000170207.
InParanoidiP21827.
KOiK11493.
OMAiPRDEAKI.
OrthoDBiEOG092C31D4.

Family and domain databases

Gene3Di2.130.10.30. 1 hit.
InterProiIPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
[Graphical view]
PfamiPF00415. RCC1. 4 hits.
[Graphical view]
PRINTSiPR00633. RCCNDNSATION.
SUPFAMiSSF50985. SSF50985. 1 hit.
PROSITEiPS00625. RCC1_1. 1 hit.
PS00626. RCC1_2. 1 hit.
PS50012. RCC1_3. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21827-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKRTVATNG DASGAHRAKK MSKTHASHII NAQEDYKHMY LSVQPLDIFC
60 70 80 90 100
WGTGSMCELG LGPLAKNKEV KRPRLNPFLP RDEAKIISFA VGGMHTLALD
110 120 130 140 150
EESNVWSWGC NDVGALGRDT SNAKEQLKDM DADDSSDDED GDLNELESTP
160 170 180 190 200
AKIPRESFPP LAEGHKVVQL AATDNMSCAL FSNGEVYAWG TFRCNEGILG
210 220 230 240 250
FYQDKIKIQK TPWKVPTFSK YNIVQLAPGK DHILFLDEEG MVFAWGNGQQ
260 270 280 290 300
NQLGRKVMER FRLKTLDPRP FGLRHVKYIA SGENHCFALT KDNKLVSWGL
310 320 330 340 350
NQFGQCGVSE DVEDGALVTK PKRLALPDNV VIRSIAAGEH HSLILSQDGD
360 370 380 390 400
LYSCGRLDMF EVGIPKDNLP EYTYKDVHGK ARAVPLPTKL NNVPKFKSVA
410 420 430 440 450
AGSHHSVAVA QNGIAYSWGF GETYAVGLGP FEDDTEVPTR IKNTATQDHN
460 470 480
IILVGCGGQF SVSGGVKLSD EDAEKRADEM DD
Length:482
Mass (Da):53,014
Last modified:May 1, 1991 - v1
Checksum:i04FFC7B9DC7DE535
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27013 Genomic DNA. Translation: AAA62268.1.
Z72619 Genomic DNA. Translation: CAA96803.1.
BK006941 Genomic DNA. Translation: DAA08009.1.
PIRiA32320. RGBYM1.
RefSeqiNP_011418.1. NM_001180962.1.

Genome annotation databases

EnsemblFungiiYGL097W; YGL097W; YGL097W.
GeneIDi852782.
KEGGisce:YGL097W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27013 Genomic DNA. Translation: AAA62268.1.
Z72619 Genomic DNA. Translation: CAA96803.1.
BK006941 Genomic DNA. Translation: DAA08009.1.
PIRiA32320. RGBYM1.
RefSeqiNP_011418.1. NM_001180962.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OF7X-ray1.90A27-482[»]
4OIHX-ray2.10B1-25[»]
5HQ2X-ray4.50K2-482[»]
ProteinModelPortaliP21827.
SMRiP21827. Positions 27-482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33153. 82 interactions.
DIPiDIP-2315N.
IntActiP21827. 3 interactions.
MINTiMINT-561451.

PTM databases

iPTMnetiP21827.

Proteomic databases

MaxQBiP21827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL097W; YGL097W; YGL097W.
GeneIDi852782.
KEGGisce:YGL097W.

Organism-specific databases

EuPathDBiFungiDB:YGL097W.
SGDiS000003065. SRM1.

Phylogenomic databases

GeneTreeiENSGT00840000129719.
HOGENOMiHOG000170207.
InParanoidiP21827.
KOiK11493.
OMAiPRDEAKI.
OrthoDBiEOG092C31D4.

Enzyme and pathway databases

BioCyciYEAST:G3O-30597-MONOMER.
ReactomeiR-SCE-3108214. SUMOylation of DNA damage response and repair proteins.

Miscellaneous databases

EvolutionaryTraceiP21827.
PROiP21827.

Family and domain databases

Gene3Di2.130.10.30. 1 hit.
InterProiIPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
[Graphical view]
PfamiPF00415. RCC1. 4 hits.
[Graphical view]
PRINTSiPR00633. RCCNDNSATION.
SUPFAMiSSF50985. SSF50985. 1 hit.
PROSITEiPS00625. RCC1_1. 1 hit.
PS00626. RCC1_2. 1 hit.
PS50012. RCC1_3. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRCC1_YEAST
AccessioniPrimary (citable) accession number: P21827
Secondary accession number(s): D6VU48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: September 7, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.