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Protein

Guanine nucleotide exchange factor SRM1

Gene

SRM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor that promotes the exchange of GSP1/GSP2-bound GDP by GTP and controls RNA metabolism and transport. Involved in yeast pheromone response pathway and in mRNA metabolism. Involved in nuclear pore complex (NPC) assembly and required for mRNA and ribosome nuclear export. Binds chromatin and is involved NPC-mediated transcriptional control.14 Publications

GO - Molecular functioni

  • Ran guanyl-nucleotide exchange factor activity Source: SGD
  • signal transducer activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • response to pheromone Source: UniProtKB-KW
  • ribosomal subunit export from nucleus Source: SGD
  • rRNA export from nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Pheromone response, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30597-MONOMER.
ReactomeiR-SCE-3108214. SUMOylation of DNA damage response and repair proteins.
R-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor SRM1
Alternative name(s):
Pheromone response pathway component SRM1
Pre-mRNA-processing protein 20
Regulator of chromosome condensation
Suppressor of receptor mutations 1
mRNA transport protein 1
Gene namesi
Name:SRM1
Synonyms:MTR1, PRP20
Ordered Locus Names:YGL097W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL097W.
SGDiS000003065. SRM1.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromatin Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19K → T: Impairs correct nuclear localization; when associated with T-20 and T-23. 1 Publication1
Mutagenesisi20K → A or Q: Impairs activity. 1 Publication1
Mutagenesisi20K → T: Impairs correct nuclear localization; when associated with T-19 and T-23. 1 Publication1
Mutagenesisi23K → T: Impairs correct nuclear localization; when associated with T-19 and T-20. 1 Publication1
Mutagenesisi282G → S: Leads to temperature-dependent mislocalization of nucleoporins (nups) and the pore-membrane protein POM152. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002066341 – 482Guanine nucleotide exchange factor SRM1Add BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated; possibly by KSP1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21827.
PRIDEiP21827.

PTM databases

iPTMnetiP21827.

Expressioni

Inductioni

By pheromone.

Interactioni

Subunit structurei

Component of a multicomponent complex composed of six to seven proteins, which has a collective molecular mass greater than 150 kDa. Interacts with GSP1 and YRB2.4 Publications

Protein-protein interaction databases

BioGridi33153. 82 interactors.
DIPiDIP-2315N.
IntActiP21827. 3 interactors.
MINTiMINT-561451.

Structurei

Secondary structure

1482
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 36Combined sources4
Helixi37 – 40Combined sources4
Beta strandi47 – 53Combined sources7
Helixi65 – 68Combined sources4
Beta strandi69 – 75Combined sources7
Turni81 – 83Combined sources3
Beta strandi86 – 91Combined sources6
Beta strandi93 – 100Combined sources8
Beta strandi105 – 109Combined sources5
Helixi145 – 148Combined sources4
Helixi155 – 157Combined sources3
Helixi159 – 162Combined sources4
Beta strandi167 – 172Combined sources6
Beta strandi174 – 181Combined sources8
Beta strandi186 – 190Combined sources5
Beta strandi192 – 194Combined sources3
Beta strandi197 – 202Combined sources6
Turni203 – 205Combined sources3
Beta strandi209 – 214Combined sources6
Beta strandi223 – 228Combined sources6
Beta strandi230 – 237Combined sources8
Beta strandi242 – 246Combined sources5
Helixi259 – 261Combined sources3
Turni262 – 265Combined sources4
Beta strandi275 – 281Combined sources7
Beta strandi283 – 290Combined sources8
Beta strandi295 – 300Combined sources6
Beta strandi317 – 323Combined sources7
Beta strandi332 – 337Combined sources6
Beta strandi339 – 346Combined sources8
Beta strandi351 – 356Combined sources6
Helixi366 – 368Combined sources3
Beta strandi381 – 389Combined sources9
Beta strandi396 – 401Combined sources6
Beta strandi403 – 410Combined sources8
Beta strandi415 – 419Combined sources5
Beta strandi435 – 440Combined sources6
Turni444 – 448Combined sources5
Beta strandi449 – 456Combined sources8
Beta strandi458 – 467Combined sources10
Helixi470 – 481Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OF7X-ray1.90A27-482[»]
4OIHX-ray2.10B1-25[»]
5HQ2X-ray4.50K2-482[»]
ProteinModelPortaliP21827.
SMRiP21827.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21827.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati45 – 101RCC1 1Add BLAST57
Repeati103 – 152RCC1 2Add BLAST50
Repeati183 – 238RCC1 3Add BLAST56
Repeati239 – 291RCC1 4Add BLAST53
Repeati292 – 347RCC1 5Add BLAST56
Repeati349 – 411RCC1 6Add BLAST63
Repeati412 – 466RCC1 7Add BLAST55

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi15 – 26Nuclear localization signal1 PublicationAdd BLAST12

Sequence similaritiesi

Contains 7 RCC1 repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00860000133726.
HOGENOMiHOG000170207.
InParanoidiP21827.
KOiK11493.
OMAiPRDEAKI.
OrthoDBiEOG092C31D4.

Family and domain databases

Gene3Di2.130.10.30. 1 hit.
InterProiIPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
[Graphical view]
PfamiPF00415. RCC1. 4 hits.
[Graphical view]
PRINTSiPR00633. RCCNDNSATION.
SUPFAMiSSF50985. SSF50985. 1 hit.
PROSITEiPS00625. RCC1_1. 1 hit.
PS00626. RCC1_2. 1 hit.
PS50012. RCC1_3. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21827-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKRTVATNG DASGAHRAKK MSKTHASHII NAQEDYKHMY LSVQPLDIFC
60 70 80 90 100
WGTGSMCELG LGPLAKNKEV KRPRLNPFLP RDEAKIISFA VGGMHTLALD
110 120 130 140 150
EESNVWSWGC NDVGALGRDT SNAKEQLKDM DADDSSDDED GDLNELESTP
160 170 180 190 200
AKIPRESFPP LAEGHKVVQL AATDNMSCAL FSNGEVYAWG TFRCNEGILG
210 220 230 240 250
FYQDKIKIQK TPWKVPTFSK YNIVQLAPGK DHILFLDEEG MVFAWGNGQQ
260 270 280 290 300
NQLGRKVMER FRLKTLDPRP FGLRHVKYIA SGENHCFALT KDNKLVSWGL
310 320 330 340 350
NQFGQCGVSE DVEDGALVTK PKRLALPDNV VIRSIAAGEH HSLILSQDGD
360 370 380 390 400
LYSCGRLDMF EVGIPKDNLP EYTYKDVHGK ARAVPLPTKL NNVPKFKSVA
410 420 430 440 450
AGSHHSVAVA QNGIAYSWGF GETYAVGLGP FEDDTEVPTR IKNTATQDHN
460 470 480
IILVGCGGQF SVSGGVKLSD EDAEKRADEM DD
Length:482
Mass (Da):53,014
Last modified:May 1, 1991 - v1
Checksum:i04FFC7B9DC7DE535
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27013 Genomic DNA. Translation: AAA62268.1.
Z72619 Genomic DNA. Translation: CAA96803.1.
BK006941 Genomic DNA. Translation: DAA08009.1.
PIRiA32320. RGBYM1.
RefSeqiNP_011418.1. NM_001180962.1.

Genome annotation databases

EnsemblFungiiYGL097W; YGL097W; YGL097W.
GeneIDi852782.
KEGGisce:YGL097W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27013 Genomic DNA. Translation: AAA62268.1.
Z72619 Genomic DNA. Translation: CAA96803.1.
BK006941 Genomic DNA. Translation: DAA08009.1.
PIRiA32320. RGBYM1.
RefSeqiNP_011418.1. NM_001180962.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OF7X-ray1.90A27-482[»]
4OIHX-ray2.10B1-25[»]
5HQ2X-ray4.50K2-482[»]
ProteinModelPortaliP21827.
SMRiP21827.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33153. 82 interactors.
DIPiDIP-2315N.
IntActiP21827. 3 interactors.
MINTiMINT-561451.

PTM databases

iPTMnetiP21827.

Proteomic databases

MaxQBiP21827.
PRIDEiP21827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL097W; YGL097W; YGL097W.
GeneIDi852782.
KEGGisce:YGL097W.

Organism-specific databases

EuPathDBiFungiDB:YGL097W.
SGDiS000003065. SRM1.

Phylogenomic databases

GeneTreeiENSGT00860000133726.
HOGENOMiHOG000170207.
InParanoidiP21827.
KOiK11493.
OMAiPRDEAKI.
OrthoDBiEOG092C31D4.

Enzyme and pathway databases

BioCyciYEAST:G3O-30597-MONOMER.
ReactomeiR-SCE-3108214. SUMOylation of DNA damage response and repair proteins.
R-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Miscellaneous databases

EvolutionaryTraceiP21827.
PROiP21827.

Family and domain databases

Gene3Di2.130.10.30. 1 hit.
InterProiIPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
[Graphical view]
PfamiPF00415. RCC1. 4 hits.
[Graphical view]
PRINTSiPR00633. RCCNDNSATION.
SUPFAMiSSF50985. SSF50985. 1 hit.
PROSITEiPS00625. RCC1_1. 1 hit.
PS00626. RCC1_2. 1 hit.
PS50012. RCC1_3. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRCC1_YEAST
AccessioniPrimary (citable) accession number: P21827
Secondary accession number(s): D6VU48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 30, 2016
This is version 162 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 12100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.