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P21826

- MASZ_YEAST

UniProt

P21826 - MASZ_YEAST

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Protein
Malate synthase 2, glyoxysomal
Gene
DAL7, MLS2, MSL2, YIR031C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This isozyme is involved in the degradation of allantoin (purine catabolism).

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771Proton acceptor By similarity
Active sitei457 – 4571Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: SGD

GO - Biological processi

  1. allantoin catabolic process Source: SGD
  2. glyoxylate cycle Source: UniProtKB-UniPathway
  3. purine nucleobase metabolic process Source: UniProtKB-KW
  4. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Purine metabolism, Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciMetaCyc:YIR031C-MONOMER.
YEAST:YIR031C-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase 2, glyoxysomal (EC:2.3.3.9)
Alternative name(s):
Degradation of allantoin protein 7
Gene namesi
Name:DAL7
Synonyms:MLS2, MSL2
Ordered Locus Names:YIR031C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

SGDiS000001470. DAL7.

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Malate synthase 2, glyoxysomal
PRO_0000166865Add
BLAST

Proteomic databases

MaxQBiP21826.
PaxDbiP21826.
PRIDEiP21826.

Expressioni

Inductioni

By allophanate or oxalurate.

Gene expression databases

GenevestigatoriP21826.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

BioGridi35022. 8 interactions.
DIPiDIP-6744N.
IntActiP21826. 1 interaction.
MINTiMINT-659799.
STRINGi4932.YIR031C.

Structurei

3D structure databases

ProteinModelPortaliP21826.
SMRiP21826. Positions 30-539.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi552 – 5543Microbody targeting signal Reviewed prediction

Sequence similaritiesi

Belongs to the malate synthase family.

Phylogenomic databases

eggNOGiCOG2225.
GeneTreeiENSGT00390000002932.
HOGENOMiHOG000238464.
KOiK01638.
OMAiRIANGRD.
OrthoDBiEOG7GXPMK.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21826-1 [UniParc]FASTAAdd to Basket

« Hide

MVKISLDNTA LYADIDTTPQ FEPSKTTVAD ILTKDALEFI VLLHRTFNST    50
RKQLLANRSN LQSKLDSGEY RFDFLPETEQ IRNDPTWQGA IPAPGLINRS 100
SEITGPPLRN MLVNALNAEV TTYMTDFEDS SSPTWENMIY GQVNLYDAIR 150
NQIDFKTPRK EYRLKDDISR LPTLIVRPRG WHMVEKHLYI DDEPISASIF 200
DFGLYFYHNA KELVKIGKGP YFYLPKMEHH MEVKLWNDIF CVAQDFIGMP 250
RGTIRATVLI ETLPAAFQME EIIYQIREHS SGLNCGRWDY IFSTIKKLRN 300
LPEHVLPNRD LVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD 350
PKANEAAMNK VRNDKIREMK NGHDGSWVAH PALAPICNEV FSNMGTANQI 400
YFVPDVHVTS SDLLNTKIQD AQVTTEGIRV NLDIGLQYME AWLRGSGCVP 450
INHLMEDAAT AEVSRCQLYQ WVKHGVVLSD TGDKVTPELT AKILNEETAK 500
LASASPLGEK NKFALAAKYF LPEVTGKIFS DFLTTLLYDE IIKPSAKPVD 550
LSKL 554
Length:554
Mass (Da):62,794
Last modified:October 5, 2010 - v2
Checksum:i006BB05371CA96CE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151A → D in AAA50351. 1 Publication
Sequence conflicti115 – 1151A → D no nucleotide entry 1 Publication
Sequence conflicti166 – 1661D → G in AAA50351. 1 Publication
Sequence conflicti166 – 1661D → G no nucleotide entry 1 Publication
Sequence conflicti302 – 3021P → N in AAA50351. 1 Publication
Sequence conflicti302 – 3021P → N no nucleotide entry 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M28124 Genomic DNA. Translation: AAA50351.1.
Z38061 Genomic DNA. Translation: CAA86191.1.
BK006942 Genomic DNA. Translation: DAA08578.1.
PIRiS48493.
RefSeqiNP_012297.3. NM_001179553.3.

Genome annotation databases

EnsemblFungiiYIR031C; YIR031C; YIR031C.
GeneIDi854849.
KEGGisce:YIR031C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M28124 Genomic DNA. Translation: AAA50351.1 .
Z38061 Genomic DNA. Translation: CAA86191.1 .
BK006942 Genomic DNA. Translation: DAA08578.1 .
PIRi S48493.
RefSeqi NP_012297.3. NM_001179553.3.

3D structure databases

ProteinModelPortali P21826.
SMRi P21826. Positions 30-539.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35022. 8 interactions.
DIPi DIP-6744N.
IntActi P21826. 1 interaction.
MINTi MINT-659799.
STRINGi 4932.YIR031C.

Proteomic databases

MaxQBi P21826.
PaxDbi P21826.
PRIDEi P21826.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIR031C ; YIR031C ; YIR031C .
GeneIDi 854849.
KEGGi sce:YIR031C.

Organism-specific databases

SGDi S000001470. DAL7.

Phylogenomic databases

eggNOGi COG2225.
GeneTreei ENSGT00390000002932.
HOGENOMi HOG000238464.
KOi K01638.
OMAi RIANGRD.
OrthoDBi EOG7GXPMK.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci MetaCyc:YIR031C-MONOMER.
YEAST:YIR031C-MONOMER.

Miscellaneous databases

NextBioi 977746.

Gene expression databases

Genevestigatori P21826.

Family and domain databases

InterProi IPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001363. Malate_synth. 1 hit.
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The DAL7 promoter consists of multiple elements that cooperatively mediate regulation of the gene's expression."
    Yoo H.S., Cooper T.G.
    Mol. Cell. Biol. 9:3231-3243(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RH218.
  2. "Two structural genes are encoding malate synthase isoenzymes in Saccharomyces cerevisiae."
    Fernandez E., Fernandez M., Rodicio R.
    FEBS Lett. 320:271-275(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiMASZ_YEAST
AccessioniPrimary (citable) accession number: P21826
Secondary accession number(s): D6VVW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

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