Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P21826

- MASZ_YEAST

UniProt

P21826 - MASZ_YEAST

Protein

Malate synthase 2, glyoxysomal

Gene

DAL7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (05 Oct 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This isozyme is involved in the degradation of allantoin (purine catabolism).

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei177 – 1771Proton acceptorBy similarity
    Active sitei457 – 4571Proton donorBy similarity

    GO - Molecular functioni

    1. malate synthase activity Source: SGD

    GO - Biological processi

    1. allantoin catabolic process Source: SGD
    2. glyoxylate cycle Source: UniProtKB-UniPathway
    3. purine nucleobase metabolic process Source: UniProtKB-KW
    4. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Purine metabolism, Tricarboxylic acid cycle

    Enzyme and pathway databases

    BioCyciMetaCyc:YIR031C-MONOMER.
    YEAST:YIR031C-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase 2, glyoxysomal (EC:2.3.3.9)
    Alternative name(s):
    Degradation of allantoin protein 7
    Gene namesi
    Name:DAL7
    Synonyms:MLS2, MSL2
    Ordered Locus Names:YIR031C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    SGDiS000001470. DAL7.

    Subcellular locationi

    GO - Cellular componenti

    1. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 554554Malate synthase 2, glyoxysomalPRO_0000166865Add
    BLAST

    Proteomic databases

    MaxQBiP21826.
    PaxDbiP21826.
    PRIDEiP21826.

    Expressioni

    Inductioni

    By allophanate or oxalurate.

    Gene expression databases

    GenevestigatoriP21826.

    Interactioni

    Subunit structurei

    Homotrimer.

    Protein-protein interaction databases

    BioGridi35022. 8 interactions.
    DIPiDIP-6744N.
    IntActiP21826. 1 interaction.
    MINTiMINT-659799.
    STRINGi4932.YIR031C.

    Structurei

    3D structure databases

    ProteinModelPortaliP21826.
    SMRiP21826. Positions 30-539.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi552 – 5543Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the malate synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG2225.
    GeneTreeiENSGT00390000002932.
    HOGENOMiHOG000238464.
    KOiK01638.
    OMAiRIANGRD.
    OrthoDBiEOG7GXPMK.

    Family and domain databases

    InterProiIPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001363. Malate_synth. 1 hit.
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
    PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P21826-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVKISLDNTA LYADIDTTPQ FEPSKTTVAD ILTKDALEFI VLLHRTFNST    50
    RKQLLANRSN LQSKLDSGEY RFDFLPETEQ IRNDPTWQGA IPAPGLINRS 100
    SEITGPPLRN MLVNALNAEV TTYMTDFEDS SSPTWENMIY GQVNLYDAIR 150
    NQIDFKTPRK EYRLKDDISR LPTLIVRPRG WHMVEKHLYI DDEPISASIF 200
    DFGLYFYHNA KELVKIGKGP YFYLPKMEHH MEVKLWNDIF CVAQDFIGMP 250
    RGTIRATVLI ETLPAAFQME EIIYQIREHS SGLNCGRWDY IFSTIKKLRN 300
    LPEHVLPNRD LVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD 350
    PKANEAAMNK VRNDKIREMK NGHDGSWVAH PALAPICNEV FSNMGTANQI 400
    YFVPDVHVTS SDLLNTKIQD AQVTTEGIRV NLDIGLQYME AWLRGSGCVP 450
    INHLMEDAAT AEVSRCQLYQ WVKHGVVLSD TGDKVTPELT AKILNEETAK 500
    LASASPLGEK NKFALAAKYF LPEVTGKIFS DFLTTLLYDE IIKPSAKPVD 550
    LSKL 554
    Length:554
    Mass (Da):62,794
    Last modified:October 5, 2010 - v2
    Checksum:i006BB05371CA96CE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti115 – 1151A → D in AAA50351. (PubMed:2552287)Curated
    Sequence conflicti115 – 1151A → D no nucleotide entry (PubMed:8462696)Curated
    Sequence conflicti166 – 1661D → G in AAA50351. (PubMed:2552287)Curated
    Sequence conflicti166 – 1661D → G no nucleotide entry (PubMed:8462696)Curated
    Sequence conflicti302 – 3021P → N in AAA50351. (PubMed:2552287)Curated
    Sequence conflicti302 – 3021P → N no nucleotide entry (PubMed:8462696)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28124 Genomic DNA. Translation: AAA50351.1.
    Z38061 Genomic DNA. Translation: CAA86191.1.
    BK006942 Genomic DNA. Translation: DAA08578.1.
    PIRiS48493.
    RefSeqiNP_012297.3. NM_001179553.3.

    Genome annotation databases

    EnsemblFungiiYIR031C; YIR031C; YIR031C.
    GeneIDi854849.
    KEGGisce:YIR031C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28124 Genomic DNA. Translation: AAA50351.1 .
    Z38061 Genomic DNA. Translation: CAA86191.1 .
    BK006942 Genomic DNA. Translation: DAA08578.1 .
    PIRi S48493.
    RefSeqi NP_012297.3. NM_001179553.3.

    3D structure databases

    ProteinModelPortali P21826.
    SMRi P21826. Positions 30-539.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35022. 8 interactions.
    DIPi DIP-6744N.
    IntActi P21826. 1 interaction.
    MINTi MINT-659799.
    STRINGi 4932.YIR031C.

    Proteomic databases

    MaxQBi P21826.
    PaxDbi P21826.
    PRIDEi P21826.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIR031C ; YIR031C ; YIR031C .
    GeneIDi 854849.
    KEGGi sce:YIR031C.

    Organism-specific databases

    SGDi S000001470. DAL7.

    Phylogenomic databases

    eggNOGi COG2225.
    GeneTreei ENSGT00390000002932.
    HOGENOMi HOG000238464.
    KOi K01638.
    OMAi RIANGRD.
    OrthoDBi EOG7GXPMK.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci MetaCyc:YIR031C-MONOMER.
    YEAST:YIR031C-MONOMER.

    Miscellaneous databases

    NextBioi 977746.

    Gene expression databases

    Genevestigatori P21826.

    Family and domain databases

    InterProi IPR011076. Malate_synth-like.
    IPR006252. Malate_synthA.
    IPR001465. Malate_synthase.
    IPR019830. Malate_synthase_CS.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001363. Malate_synth. 1 hit.
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01344. malate_syn_A. 1 hit.
    PROSITEi PS00510. MALATE_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DAL7 promoter consists of multiple elements that cooperatively mediate regulation of the gene's expression."
      Yoo H.S., Cooper T.G.
      Mol. Cell. Biol. 9:3231-3243(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RH218.
    2. "Two structural genes are encoding malate synthase isoenzymes in Saccharomyces cerevisiae."
      Fernandez E., Fernandez M., Rodicio R.
      FEBS Lett. 320:271-275(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiMASZ_YEAST
    AccessioniPrimary (citable) accession number: P21826
    Secondary accession number(s): D6VVW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3