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Protein

Malate synthase 2, glyoxysomal

Gene

DAL7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This isozyme is involved in the degradation of allantoin (purine catabolism).

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei177 – 1771Proton acceptorBy similarity
Active sitei457 – 4571Proton donorBy similarity

GO - Molecular functioni

  1. malate synthase activity Source: SGD

GO - Biological processi

  1. allantoin catabolic process Source: SGD
  2. glyoxylate cycle Source: UniProtKB-UniPathway
  3. purine nucleobase metabolic process Source: UniProtKB-KW
  4. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Purine metabolism, Tricarboxylic acid cycle

Enzyme and pathway databases

BioCyciMetaCyc:YIR031C-MONOMER.
YEAST:YIR031C-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase 2, glyoxysomal (EC:2.3.3.9)
Alternative name(s):
Degradation of allantoin protein 7
Gene namesi
Name:DAL7
Synonyms:MLS2, MSL2
Ordered Locus Names:YIR031C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IX

Organism-specific databases

SGDiS000001470. DAL7.

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 554554Malate synthase 2, glyoxysomalPRO_0000166865Add
BLAST

Proteomic databases

MaxQBiP21826.
PaxDbiP21826.
PRIDEiP21826.

Expressioni

Inductioni

By allophanate or oxalurate.

Gene expression databases

GenevestigatoriP21826.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

BioGridi35022. 8 interactions.
DIPiDIP-6744N.
IntActiP21826. 1 interaction.
MINTiMINT-659799.
STRINGi4932.YIR031C.

Structurei

3D structure databases

ProteinModelPortaliP21826.
SMRiP21826. Positions 30-539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi552 – 5543Microbody targeting signalSequence Analysis

Sequence similaritiesi

Belongs to the malate synthase family.Curated

Phylogenomic databases

eggNOGiCOG2225.
GeneTreeiENSGT00390000002932.
HOGENOMiHOG000238464.
InParanoidiP21826.
KOiK01638.
OMAiMESRHEA.
OrthoDBiEOG7GXPMK.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P21826-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKISLDNTA LYADIDTTPQ FEPSKTTVAD ILTKDALEFI VLLHRTFNST
60 70 80 90 100
RKQLLANRSN LQSKLDSGEY RFDFLPETEQ IRNDPTWQGA IPAPGLINRS
110 120 130 140 150
SEITGPPLRN MLVNALNAEV TTYMTDFEDS SSPTWENMIY GQVNLYDAIR
160 170 180 190 200
NQIDFKTPRK EYRLKDDISR LPTLIVRPRG WHMVEKHLYI DDEPISASIF
210 220 230 240 250
DFGLYFYHNA KELVKIGKGP YFYLPKMEHH MEVKLWNDIF CVAQDFIGMP
260 270 280 290 300
RGTIRATVLI ETLPAAFQME EIIYQIREHS SGLNCGRWDY IFSTIKKLRN
310 320 330 340 350
LPEHVLPNRD LVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD
360 370 380 390 400
PKANEAAMNK VRNDKIREMK NGHDGSWVAH PALAPICNEV FSNMGTANQI
410 420 430 440 450
YFVPDVHVTS SDLLNTKIQD AQVTTEGIRV NLDIGLQYME AWLRGSGCVP
460 470 480 490 500
INHLMEDAAT AEVSRCQLYQ WVKHGVVLSD TGDKVTPELT AKILNEETAK
510 520 530 540 550
LASASPLGEK NKFALAAKYF LPEVTGKIFS DFLTTLLYDE IIKPSAKPVD

LSKL
Length:554
Mass (Da):62,794
Last modified:October 4, 2010 - v2
Checksum:i006BB05371CA96CE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151A → D in AAA50351 (PubMed:2552287).Curated
Sequence conflicti115 – 1151A → D no nucleotide entry (PubMed:8462696).Curated
Sequence conflicti166 – 1661D → G in AAA50351 (PubMed:2552287).Curated
Sequence conflicti166 – 1661D → G no nucleotide entry (PubMed:8462696).Curated
Sequence conflicti302 – 3021P → N in AAA50351 (PubMed:2552287).Curated
Sequence conflicti302 – 3021P → N no nucleotide entry (PubMed:8462696).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28124 Genomic DNA. Translation: AAA50351.1.
Z38061 Genomic DNA. Translation: CAA86191.1.
BK006942 Genomic DNA. Translation: DAA08578.1.
PIRiS48493.
RefSeqiNP_012297.3. NM_001179553.3.

Genome annotation databases

EnsemblFungiiYIR031C; YIR031C; YIR031C.
GeneIDi854849.
KEGGisce:YIR031C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28124 Genomic DNA. Translation: AAA50351.1.
Z38061 Genomic DNA. Translation: CAA86191.1.
BK006942 Genomic DNA. Translation: DAA08578.1.
PIRiS48493.
RefSeqiNP_012297.3. NM_001179553.3.

3D structure databases

ProteinModelPortaliP21826.
SMRiP21826. Positions 30-539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35022. 8 interactions.
DIPiDIP-6744N.
IntActiP21826. 1 interaction.
MINTiMINT-659799.
STRINGi4932.YIR031C.

Proteomic databases

MaxQBiP21826.
PaxDbiP21826.
PRIDEiP21826.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIR031C; YIR031C; YIR031C.
GeneIDi854849.
KEGGisce:YIR031C.

Organism-specific databases

SGDiS000001470. DAL7.

Phylogenomic databases

eggNOGiCOG2225.
GeneTreeiENSGT00390000002932.
HOGENOMiHOG000238464.
InParanoidiP21826.
KOiK01638.
OMAiMESRHEA.
OrthoDBiEOG7GXPMK.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciMetaCyc:YIR031C-MONOMER.
YEAST:YIR031C-MONOMER.

Miscellaneous databases

NextBioi977746.

Gene expression databases

GenevestigatoriP21826.

Family and domain databases

InterProiIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001363. Malate_synth. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01344. malate_syn_A. 1 hit.
PROSITEiPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DAL7 promoter consists of multiple elements that cooperatively mediate regulation of the gene's expression."
    Yoo H.S., Cooper T.G.
    Mol. Cell. Biol. 9:3231-3243(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RH218.
  2. "Two structural genes are encoding malate synthase isoenzymes in Saccharomyces cerevisiae."
    Fernandez E., Fernandez M., Rodicio R.
    FEBS Lett. 320:271-275(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiMASZ_YEAST
AccessioniPrimary (citable) accession number: P21826
Secondary accession number(s): D6VVW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 1991
Last sequence update: October 4, 2010
Last modified: January 6, 2015
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.