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P21826 (MASZ_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase 2, glyoxysomal

EC=2.3.3.9
Alternative name(s):
Degradation of allantoin protein 7
Gene names
Name:DAL7
Synonyms:MLS2, MSL2
Ordered Locus Names:YIR031C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This isozyme is involved in the degradation of allantoin (purine catabolism).

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Subunit structure

Homotrimer.

Subcellular location

Glyoxysome.

Induction

By allophanate or oxalurate.

Sequence similarities

Belongs to the malate synthase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 554554Malate synthase 2, glyoxysomal
PRO_0000166865

Regions

Motif552 – 5543Microbody targeting signal Potential

Sites

Active site1771Proton acceptor By similarity
Active site4571Proton donor By similarity

Experimental info

Sequence conflict1151A → D in AAA50351. Ref.1
Sequence conflict1151A → D no nucleotide entry Ref.2
Sequence conflict1661D → G in AAA50351. Ref.1
Sequence conflict1661D → G no nucleotide entry Ref.2
Sequence conflict3021P → N in AAA50351. Ref.1
Sequence conflict3021P → N no nucleotide entry Ref.2

Sequences

Sequence LengthMass (Da)Tools
P21826 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 006BB05371CA96CE

FASTA55462,794
        10         20         30         40         50         60 
MVKISLDNTA LYADIDTTPQ FEPSKTTVAD ILTKDALEFI VLLHRTFNST RKQLLANRSN 

        70         80         90        100        110        120 
LQSKLDSGEY RFDFLPETEQ IRNDPTWQGA IPAPGLINRS SEITGPPLRN MLVNALNAEV 

       130        140        150        160        170        180 
TTYMTDFEDS SSPTWENMIY GQVNLYDAIR NQIDFKTPRK EYRLKDDISR LPTLIVRPRG 

       190        200        210        220        230        240 
WHMVEKHLYI DDEPISASIF DFGLYFYHNA KELVKIGKGP YFYLPKMEHH MEVKLWNDIF 

       250        260        270        280        290        300 
CVAQDFIGMP RGTIRATVLI ETLPAAFQME EIIYQIREHS SGLNCGRWDY IFSTIKKLRN 

       310        320        330        340        350        360 
LPEHVLPNRD LVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD PKANEAAMNK 

       370        380        390        400        410        420 
VRNDKIREMK NGHDGSWVAH PALAPICNEV FSNMGTANQI YFVPDVHVTS SDLLNTKIQD 

       430        440        450        460        470        480 
AQVTTEGIRV NLDIGLQYME AWLRGSGCVP INHLMEDAAT AEVSRCQLYQ WVKHGVVLSD 

       490        500        510        520        530        540 
TGDKVTPELT AKILNEETAK LASASPLGEK NKFALAAKYF LPEVTGKIFS DFLTTLLYDE 

       550 
IIKPSAKPVD LSKL 

« Hide

References

« Hide 'large scale' references
[1]"The DAL7 promoter consists of multiple elements that cooperatively mediate regulation of the gene's expression."
Yoo H.S., Cooper T.G.
Mol. Cell. Biol. 9:3231-3243(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: RH218.
[2]"Two structural genes are encoding malate synthase isoenzymes in Saccharomyces cerevisiae."
Fernandez E., Fernandez M., Rodicio R.
FEBS Lett. 320:271-275(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28124 Genomic DNA. Translation: AAA50351.1.
Z38061 Genomic DNA. Translation: CAA86191.1.
BK006942 Genomic DNA. Translation: DAA08578.1.
PIRS48493.
RefSeqNP_012297.3. NM_001179553.3.

3D structure databases

ProteinModelPortalP21826.
SMRP21826. Positions 30-539.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35022. 8 interactions.
DIPDIP-6744N.
IntActP21826. 1 interaction.
MINTMINT-659799.
STRING4932.YIR031C.

Proteomic databases

MaxQBP21826.
PaxDbP21826.
PRIDEP21826.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIR031C; YIR031C; YIR031C.
GeneID854849.
KEGGsce:YIR031C.

Organism-specific databases

SGDS000001470. DAL7.

Phylogenomic databases

eggNOGCOG2225.
GeneTreeENSGT00390000002932.
HOGENOMHOG000238464.
KOK01638.
OMARIANGRD.
OrthoDBEOG7GXPMK.

Enzyme and pathway databases

BioCycMetaCyc:YIR031C-MONOMER.
YEAST:YIR031C-MONOMER.
UniPathwayUPA00703; UER00720.

Gene expression databases

GenevestigatorP21826.

Family and domain databases

InterProIPR011076. Malate_synth-like.
IPR006252. Malate_synthA.
IPR001465. Malate_synthase.
IPR019830. Malate_synthase_CS.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001363. Malate_synth. 1 hit.
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01344. malate_syn_A. 1 hit.
PROSITEPS00510. MALATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977746.

Entry information

Entry nameMASZ_YEAST
AccessionPrimary (citable) accession number: P21826
Secondary accession number(s): D6VVW2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways