P21826 (MASZ_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase 2, glyoxysomal
EC=2.3.3.9

Alternative name(s):
Degradation of allantoin protein 7

Gene names

Name:	DAL7
Synonyms:	MLS2, MSL2
Ordered Locus Names:	YIR031C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	554 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This isozyme is involved in the degradation of allantoin (purine catabolism).
Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Subunit structure	Homotrimer.
Subcellular location	Glyoxysome.
Induction	By allophanate or oxalurate.
Sequence similarities	Belongs to the malate synthase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Purine metabolism Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	allantoin catabolic process Inferred from direct assay Ref.2. Source: SGD glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from direct assay Ref.2. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 554	554	Malate synthase 2, glyoxysomal		PRO_0000166865
Regions
	Motif	552 – 554	3	Microbody targeting signal Potential
Sites
	Active site	177	1	Proton acceptor By similarity
	Active site	457	1	Proton donor By similarity
Experimental info
	Sequence conflict	115	1	A → D in AAA50351. Ref.1
	Sequence conflict	115	1	A → D no nucleotide entry Ref.2
	Sequence conflict	166	1	D → G in AAA50351. Ref.1
	Sequence conflict	166	1	D → G no nucleotide entry Ref.2
	Sequence conflict	302	1	P → N in AAA50351. Ref.1
	Sequence conflict	302	1	P → N no nucleotide entry Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P21826 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: 006BB05371CA96CE	FASTA	554	62,794
	10 20 30 40 50 60 MVKISLDNTA LYADIDTTPQ FEPSKTTVAD ILTKDALEFI VLLHRTFNST RKQLLANRSN 70 80 90 100 110 120 LQSKLDSGEY RFDFLPETEQ IRNDPTWQGA IPAPGLINRS SEITGPPLRN MLVNALNAEV 130 140 150 160 170 180 TTYMTDFEDS SSPTWENMIY GQVNLYDAIR NQIDFKTPRK EYRLKDDISR LPTLIVRPRG 190 200 210 220 230 240 WHMVEKHLYI DDEPISASIF DFGLYFYHNA KELVKIGKGP YFYLPKMEHH MEVKLWNDIF 250 260 270 280 290 300 CVAQDFIGMP RGTIRATVLI ETLPAAFQME EIIYQIREHS SGLNCGRWDY IFSTIKKLRN 310 320 330 340 350 360 LPEHVLPNRD LVTMTSPFMD AYVKRLINTC HRRGVHAMGG MAAQIPIKDD PKANEAAMNK 370 380 390 400 410 420 VRNDKIREMK NGHDGSWVAH PALAPICNEV FSNMGTANQI YFVPDVHVTS SDLLNTKIQD 430 440 450 460 470 480 AQVTTEGIRV NLDIGLQYME AWLRGSGCVP INHLMEDAAT AEVSRCQLYQ WVKHGVVLSD 490 500 510 520 530 540 TGDKVTPELT AKILNEETAK LASASPLGEK NKFALAAKYF LPEVTGKIFS DFLTTLLYDE 550 IIKPSAKPVD LSKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DAL7 promoter consists of multiple elements that cooperatively mediate regulation of the gene's expression." Yoo H.S., Cooper T.G. Mol. Cell. Biol. 9:3231-3243(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RH218.
[2]	"Two structural genes are encoding malate synthase isoenzymes in Saccharomyces cerevisiae." Fernandez E., Fernandez M., Rodicio R. FEBS Lett. 320:271-275(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M28124 Genomic DNA. Translation: AAA50351.1. Z38061 Genomic DNA. Translation: CAA86191.1. BK006942 Genomic DNA. Translation: DAA08578.1.
PIR	S48493.
RefSeq	NP_012297.3. NM_001179553.3.
3D structure databases
ProteinModelPortal	P21826.
SMR	P21826. Positions 30-539.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6744N.
IntAct	P21826. 1 interaction.
MINT	MINT-659799.
STRING	4932.YIR031C.
Proteomic databases
PaxDb	P21826.
PRIDE	P21826.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIR031C; YIR031C; YIR031C.
GeneID	854849.
KEGG	sce:YIR031C. sce:YIR039C.
Organism-specific databases
SGD	S000001470. DAL7.
Phylogenomic databases
eggNOG	COG2225.
GeneTree	ENSGT00390000002932.
HOGENOM	HOG000238464.
KO	K01386. K01638.
OMA	IRADESW.
OrthoDB	EOG4TXG13.
Enzyme and pathway databases
UniPathway	UPA00703; UER00720.
Gene expression databases
Genevestigator	P21826.
GermOnline	YIR031C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011076. Malate_synth-like. IPR006252. Malate_synthA. IPR001465. Malate_synthase. IPR019830. Malate_synthase_CS. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF001363. Malate_synth. 1 hit.
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01344. malate_syn_A. 1 hit.
PROSITE	PS00510. MALATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977746.

Entry information

Entry name

MASZ_YEAST

Accession

Primary (citable) accession number: P21826
Secondary accession number(s): D6VVW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	October 5, 2010
Last modified:	May 1, 2013
This is version 121 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program