P21818 (STMN2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Stathmin-2

Alternative name(s):
Superior cervical ganglion-10 protein
Short name=Protein SCG10

Gene names

Name:	Stmn2
Synonyms:	Scg10, Scgn10

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone. Ref.4 Ref.5 Ref.6
Subunit structure	Interacts with ITM2C By similarity. Interacts with MAPK8. Ref.5
Subcellular location	Cytoplasm. Cytoplasm › perinuclear region. Cell projection › growth cone. Cell projection › axon. Membrane; Peripheral membrane protein; Cytoplasmic side Potential. Golgi apparatus. Endosome. Note: Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. Exists in both soluble and membrane-bound forms. Ref.5
Tissue specificity	Present in growth cones and abundant in developing neurons.
Developmental stage	In the developing cerebellum, maximal levels occur at postnatal day 7 and correlate with the onset of neuronal differentiation.
Induction	By nerve growth factor.
Post-translational modification	Sumoylated By similarity. Pphosphorylated by MAPK9 and MAPK10 in the developing brain cortex By similarity. Phosphorylated mostly by MAPK8. Ref.5 N-terminal palmitoylation promotes specific anchoring to the cytosolic leaflet of Golgi membranes and subsequent vesicular trafficking along dendrites and axons. Neuronal Stathmins are substrates for palmitoyltransferases ZDHHC3, ZDHHC7 and ZDHHC15 By similarity.
Sequence similarities	Belongs to the stathmin family. Contains 1 SLD (stathmin-like) domain.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Endosome Golgi apparatus Membrane
Domain	Coiled coil
PTM	Lipoprotein Palmitate Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of microtubule polymerization or depolymerization Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from direct assay Ref.5. Source: RGD axon Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from electronic annotation. Source: UniProtKB-SubCell growth cone Inferred from direct assay Ref.5. Source: RGD membrane Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell vesicle Inferred from direct assay Ref.5. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Stathmin-2		PRO_0000182398
Regions
	Domain	38 – 179	142	SLD
	Region	1 – 26	26	Membrane attachment Potential
	Region	39 – 96	58	Regulatory/phosphorylation domain Potential
	Coiled coil	75 – 179	105	Potential
Amino acid modifications
	Modified residue	16	1	Phosphoserine Potential
	Modified residue	50	1	Phosphoserine Potential
	Modified residue	62	1	Phosphoserine; by MAPK8 Ref.5
	Modified residue	73	1	Phosphoserine; by MAPK8 Ref.5
	Modified residue	80	1	Phosphoserine Potential
	Modified residue	97	1	Phosphoserine Potential
	Lipidation	22	1	S-palmitoyl cysteine By similarity
	Lipidation	24	1	S-palmitoyl cysteine By similarity
Experimental info
	Mutagenesis	62	1	S → A: Partial loss of phosphorylation. Complete loss of phosphorylation by MAPK8, increased microtubule solubility, and reduced neurite length; when associated with A-73. Ref.5
	Mutagenesis	62	1	S → D: Increased microtubule stability but no effect on neurite length; when associated with A-73. Ref.5
	Mutagenesis	73	1	S → A: Partial loss of phosphorylation. Complete loss of phosphorylation by MAPK8, increased microtubule solubility, and reduced neurite length; when associated with A-62. Ref.5
	Mutagenesis	73	1	S → D: Increased microtubule stability but no effect on neurite length; when associated with A-62. Ref.5
	Sequence conflict	59	1	K → L no nucleotide entry Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P21818 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: 8B827AA3977E61B8	FASTA	179	20,756
	10 20 30 40 50 60 MAKTAMAYKE KMKELSMLSL ICSCFYPEPR NINIYTYDDM EVKQINKRAS GQAFELILKP 70 80 90 100 110 120 PSPISEAPRT LASPKKKDLS LEEIQKKLEA AEGRRKSQEA QVLKQLAEKR EHEREVLQKA 130 140 150 160 170 LEENNNFSKM AEEKLILKME QIKENREANL AAIIERLQEK ERHAAEVRRN KELQVELSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The NGF-inducible SCG10 mRNA encodes a novel membrane-bound protein present in growth cones and abundant in developing neurons." Stein R., Mori N., Matthews K., Lo L.-C., Anderson D.J. Neuron 1:463-476(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Novel genes expressed in rat dorsal root ganglion." Xiao H., Huang Q., Zhang F., Yang Z., Chen Z., Han Z., Zhang X. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Spinal ganglion.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Regulation of microtubule dynamics by the neuronal growth-associated protein SCG10." Riederer B.M., Pellier V., Antonsson B., Di Paolo G., Stimpson S.A., Luetjens R., Catsicas S., Grenningloh G. Proc. Natl. Acad. Sci. U.S.A. 94:741-745(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"JNK1 phosphorylation of SCG10 determines microtubule dynamics and axodendritic length." Tararuk T., Ostman N., Li W., Bjorkblom B., Padzik A., Zdrojewska J., Hongisto V., Herdegen T., Konopka W., Courtney M.J., Coffey E.T. J. Cell Biol. 173:265-277(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-62 AND SER-73, INTERACTION WITH MAPK8, MUTAGENESIS OF SER-62 AND SER-73.
[6]	"Phosphorylation of SCG10/stathmin-2 determines multipolar stage exit and neuronal migration rate." Westerlund N., Zdrojewska J., Padzik A., Komulainen E., Bjorkblom B., Rannikko E., Tararuk T., Garcia-Frigola C., Sandholm J., Nguyen L., Kallunki T., Courtney M.J., Coffey E.T. Nat. Neurosci. 14:305-313(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF306458 mRNA. Translation: AAG33230.1. BC087660 mRNA. Translation: AAH87660.1.
IPI	IPI00326596.
PIR	A36110.
RefSeq	NP_445892.1. NM_053440.2.
UniGene	Rn.34335.
3D structure databases
ProteinModelPortal	P21818.
SMR	P21818. Positions 40-174.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000056024.
PTM databases
PhosphoSite	P21818.
Proteomic databases
PaxDb	P21818.
PRIDE	P21818.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000015720; ENSRNOP00000015720; ENSRNOG00000011705.
GeneID	84510.
KEGG	rno:84510.
UCSC	RGD:68947. rat.
Organism-specific databases
CTD	11075.
RGD	68947. Stmn2.
Phylogenomic databases
eggNOG	NOG71188.
GeneTree	ENSGT00390000003691.
HOGENOM	HOG000013197.
HOVERGEN	HBG054037.
OrthoDB	EOG4DR9DK.
Gene expression databases
ArrayExpress	P21818.
Genevestigator	P21818.
GermOnline	ENSRNOG00000011705. Rattus norvegicus.
Family and domain databases
InterPro	IPR026729. Stathmin-2. IPR000956. Stathmin_fam. [Graphical view]
PANTHER	PTHR10104. PTHR10104. 1 hit. PTHR10104:SF7. PTHR10104:SF7. 1 hit.
Pfam	PF00836. Stathmin. 1 hit. [Graphical view]
PIRSF	PIRSF002285. Stathmin. 1 hit.
PRINTS	PR00345. STATHMIN.
SUPFAM	SSF101494. Stathmin. 1 hit.
PROSITE	PS00563. STATHMIN_1. 1 hit. PS01041. STATHMIN_2. 1 hit. PS51663. STATHMIN_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	617089.

Entry information

Entry name

STMN2_RAT

Accession

Primary (citable) accession number: P21818
Secondary accession number(s): Q9ERH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	April 13, 2004
Last modified:	April 3, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program