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P21818 (STMN2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Stathmin-2
Alternative name(s):
Superior cervical ganglion-10 protein
Short name=Protein SCG10
Gene names
Name:Stmn2
Synonyms:Scg10, Scgn10
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length179 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone. Ref.4 Ref.5 Ref.7

Subunit structure

Interacts with ITM2C By similarity. Interacts with MAPK8. Interacts with KIAA1279 By similarity. Interacts (via the N-terminal region) with CIB1 (via C-terminal region); the interaction is direct, occurs in a calcium-dependent manner and attenuates the neurite outgrowth inhibition of STMN2 By similarity. Ref.5

Subcellular location

Cytoplasm. Cytoplasmperinuclear region. Cell projectiongrowth cone. Cell projectionaxon. Membrane; Peripheral membrane protein; Cytoplasmic side Potential. Golgi apparatus. Endosome. Cell projectionlamellipodium By similarity. Note: Colocalized with CIB1 in the cell body, neuritis and growth cones of neurons. Colocalized with CIB1 to the leading edge of lamellipodia By similarity. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. Exists in both soluble and membrane-bound forms. Colocalized with CIB1 in neurites of developing hippocampal primary neurons. Ref.5 Ref.6

Tissue specificity

Expressed in neurons (at protein level). Present in growth cones and abundant in developing neurons. Ref.6

Developmental stage

In the developing cerebellum, maximal levels occur at postnatal day 7 and correlate with the onset of neuronal differentiation.

Induction

By nerve growth factor.

Post-translational modification

Sumoylated By similarity.

Pphosphorylated by MAPK9 and MAPK10 in the developing brain cortex By similarity. Phosphorylated mostly by MAPK8. Ref.5

N-terminal palmitoylation promotes specific anchoring to the cytosolic leaflet of Golgi membranes and subsequent vesicular trafficking along dendrites and axons. Neuronal Stathmins are substrates for palmitoyltransferases ZDHHC3, ZDHHC7 and ZDHHC15 By similarity.

Sequence similarities

Belongs to the stathmin family.

Contains 1 SLD (stathmin-like) domain.

Ontologies

Keywords
   Cellular componentCell projection
Cytoplasm
Endosome
Golgi apparatus
Membrane
   DomainCoiled coil
   PTMLipoprotein
Palmitate
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to nerve growth factor stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of microtubule depolymerization

Inferred from electronic annotation. Source: Ensembl

negative regulation of microtubule polymerization

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of neuron projection development

Inferred from electronic annotation. Source: Ensembl

positive regulation of microtubule depolymerization

Inferred from electronic annotation. Source: Ensembl

positive regulation of neuron projection development

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentGolgi apparatus

Inferred from direct assay Ref.5. Source: RGD

axon

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

endosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

growth cone

Inferred from direct assay Ref.5. Source: RGD

lamellipodium

Inferred from sequence or structural similarity. Source: UniProtKB

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

neuron projection

Inferred from sequence or structural similarity. Source: UniProtKB

neuronal cell body

Inferred from sequence or structural similarity. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

vesicle

Inferred from direct assay Ref.5. Source: RGD

   Molecular_functionprotein binding

Inferred from physical interaction Ref.5. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 179179Stathmin-2
PRO_0000182398

Regions

Domain38 – 179142SLD
Region1 – 2626Membrane attachment Potential
Region39 – 9658Regulatory/phosphorylation domain Potential
Coiled coil75 – 179105 Potential

Amino acid modifications

Modified residue161Phosphoserine Potential
Modified residue501Phosphoserine Potential
Modified residue621Phosphoserine; by MAPK8 Ref.5
Modified residue731Phosphoserine; by MAPK8 Ref.5
Modified residue801Phosphoserine Potential
Modified residue971Phosphoserine Potential
Lipidation221S-palmitoyl cysteine By similarity
Lipidation241S-palmitoyl cysteine By similarity

Experimental info

Mutagenesis621S → A: Partial loss of phosphorylation. Complete loss of phosphorylation by MAPK8, increased microtubule solubility, and reduced neurite length; when associated with A-73. Ref.5
Mutagenesis621S → D: Increased microtubule stability but no effect on neurite length; when associated with A-73. Ref.5
Mutagenesis731S → A: Partial loss of phosphorylation. Complete loss of phosphorylation by MAPK8, increased microtubule solubility, and reduced neurite length; when associated with A-62. Ref.5
Mutagenesis731S → D: Increased microtubule stability but no effect on neurite length; when associated with A-62. Ref.5
Sequence conflict591K → L no nucleotide entry Ref.1

Sequences

Sequence LengthMass (Da)Tools
P21818 [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: 8B827AA3977E61B8

FASTA17920,756
        10         20         30         40         50         60 
MAKTAMAYKE KMKELSMLSL ICSCFYPEPR NINIYTYDDM EVKQINKRAS GQAFELILKP 

        70         80         90        100        110        120 
PSPISEAPRT LASPKKKDLS LEEIQKKLEA AEGRRKSQEA QVLKQLAEKR EHEREVLQKA 

       130        140        150        160        170 
LEENNNFSKM AEEKLILKME QIKENREANL AAIIERLQEK ERHAAEVRRN KELQVELSG 

« Hide

References

« Hide 'large scale' references
[1]"The NGF-inducible SCG10 mRNA encodes a novel membrane-bound protein present in growth cones and abundant in developing neurons."
Stein R., Mori N., Matthews K., Lo L.-C., Anderson D.J.
Neuron 1:463-476(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Novel genes expressed in rat dorsal root ganglion."
Xiao H., Huang Q., Zhang F., Yang Z., Chen Z., Han Z., Zhang X.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Spinal ganglion.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Regulation of microtubule dynamics by the neuronal growth-associated protein SCG10."
Riederer B.M., Pellier V., Antonsson B., Di Paolo G., Stimpson S.A., Luetjens R., Catsicas S., Grenningloh G.
Proc. Natl. Acad. Sci. U.S.A. 94:741-745(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"JNK1 phosphorylation of SCG10 determines microtubule dynamics and axodendritic length."
Tararuk T., Ostman N., Li W., Bjorkblom B., Padzik A., Zdrojewska J., Hongisto V., Herdegen T., Konopka W., Courtney M.J., Coffey E.T.
J. Cell Biol. 173:265-277(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-62 AND SER-73, INTERACTION WITH MAPK8, MUTAGENESIS OF SER-62 AND SER-73.
[6]"Calmyrin1 binds to SCG10 protein (stathmin2) to modulate neurite outgrowth."
Sobczak A., Debowska K., Blazejczyk M., Kreutz M.R., Kuznicki J., Wojda U.
Biochim. Biophys. Acta 1813:1025-1037(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"Phosphorylation of SCG10/stathmin-2 determines multipolar stage exit and neuronal migration rate."
Westerlund N., Zdrojewska J., Padzik A., Komulainen E., Bjorkblom B., Rannikko E., Tararuk T., Garcia-Frigola C., Sandholm J., Nguyen L., Kallunki T., Courtney M.J., Coffey E.T.
Nat. Neurosci. 14:305-313(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF306458 mRNA. Translation: AAG33230.1.
BC087660 mRNA. Translation: AAH87660.1.
PIRA36110.
RefSeqNP_445892.1. NM_053440.2.
UniGeneRn.34335.

3D structure databases

ProteinModelPortalP21818.
SMRP21818. Positions 40-174.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000056024.

PTM databases

PhosphoSiteP21818.

Proteomic databases

PaxDbP21818.
PRIDEP21818.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000015720; ENSRNOP00000015720; ENSRNOG00000011705.
GeneID84510.
KEGGrno:84510.
UCSCRGD:68947. rat.

Organism-specific databases

CTD11075.
RGD68947. Stmn2.

Phylogenomic databases

eggNOGNOG71188.
GeneTreeENSGT00390000003691.
HOGENOMHOG000013197.
HOVERGENHBG054037.
OMADQIKENR.
OrthoDBEOG744TBN.

Gene expression databases

GenevestigatorP21818.

Family and domain databases

InterProIPR026729. Stathmin-2.
IPR000956. Stathmin_fam.
[Graphical view]
PANTHERPTHR10104. PTHR10104. 1 hit.
PTHR10104:SF18. PTHR10104:SF18. 1 hit.
PfamPF00836. Stathmin. 1 hit.
[Graphical view]
PIRSFPIRSF002285. Stathmin. 1 hit.
PRINTSPR00345. STATHMIN.
SUPFAMSSF101494. SSF101494. 1 hit.
PROSITEPS00563. STATHMIN_1. 1 hit.
PS01041. STATHMIN_2. 1 hit.
PS51663. STATHMIN_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio617089.
PROP21818.

Entry information

Entry nameSTMN2_RAT
AccessionPrimary (citable) accession number: P21818
Secondary accession number(s): Q9ERH2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: April 13, 2004
Last modified: June 11, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families