Cysteine dioxygenase type 1 - Rattus norvegicus (Rat)

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P21816 (CDO1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cysteine dioxygenase type 1
EC=1.13.11.20

Alternative name(s):
Cysteine dioxygenase type I
Short name=CDO
Short name=CDO-I

Gene names

Name:

Cdo1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-cysteine + O₂ = 3-sulfinoalanine.
Cofactor	Binds 1 iron ion per subunit.
Pathway	Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.
Subunit structure	Monomer.
Tissue specificity	Highest levels in liver. Significant expression also in kidney, lung and brain. Ref.2
Developmental stage	From neonate to weaning.
Induction	By increase of sulfur amino acid intake.
Post-translational modification	The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe²⁺ ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.
Sequence similarities	Belongs to the cysteine dioxygenase family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Thioether bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	L-cysteine catabolic process Inferred from direct assay PubMed 16611641. Source: RGD lactation Inferred from direct assay PubMed 6509133. Source: RGD response to amino acid stimulus Inferred from direct assay PubMed 11287364. Source: RGD response to cAMP Inferred from direct assay PubMed 212416. Source: RGD response to ethanol Inferred from direct assay PubMed 12908607. Source: RGD response to glucagon stimulus Inferred from direct assay PubMed 212416. Source: RGD response to glucocorticoid stimulus Inferred from direct assay PubMed 7419499. Source: RGD taurine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Traceable author statement Ref.1. Source: RGD
Molecular_function	cysteine dioxygenase activity Inferred from direct assay PubMed 9824269. Source: UniProtKB ferrous iron binding Inferred from direct assay PubMed 16611641. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 200

200

Cysteine dioxygenase type 1

PRO_0000206609

Sites

Metal binding

Iron; catalytic

Metal binding

Iron; catalytic

Metal binding

140

Iron; catalytic

Amino acid modifications

Cross-link

93 ↔ 157

3'-(S-cysteinyl)-tyrosine (Cys-Tyr)

Experimental info

Sequence conflict

T → S in AAH70509. Ref.3

Secondary structure

200

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P21816 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: 118F1A3326B340F9
Show »

FASTA

200

23,026

        10         20         30         40         50         60 
MERTELLKPR TLADLIRILH ELFAGDEVNV EEVQAVLEAY ESNPAEWALY AKFDQYRYTR 

        70         80         90        100        110        120 
NLVDQGNGKF NLMILCWGEG HGSSIHDHTD SHCFLKLLQG NLKETLFDWP DKKSNEMIKK 

       130        140        150        160        170        180 
SERTLRENQC AYINDSIGLH RVENVSHTEP AVSLHLYSPP FDTCHAFDQR TGHKNKVTMT 

       190        200 
FHSKFGIRTP FTTSGSLENN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a cDNA for rat liver cysteine dioxygenase." Hosokawa Y., Matsumoto A., Oka J., Itakura H., Yamaguchi K. Biochem. Biophys. Res. Commun. 168:473-478(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Structural organization and tissue-specific expression of the gene encoding rat cysteine dioxygenase." Tsuboyama N., Hosokawa Y., Totani M., Oka J., Matsumoto A., Koide T., Kodama H. Gene 181:161-165(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[4]	"Crystal structure of mammalian cysteine dioxygenase. A novel mononuclear iron center for cysteine thiol oxidation." Simmons C.R., Liu Q., Huang Q., Hao Q., Begley T.P., Karplus P.A., Stipanuk M.H. J. Biol. Chem. 281:18723-18733(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH IRON, CROSS-LINK.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M35266 mRNA. Translation: AAA40904.1.
D83481 Genomic DNA. Translation: BAA11925.1.
BC070509 mRNA. Translation: AAH70509.1.

IPI

IPI00331764.

PIR

A34632.

RefSeq

NP_434696.1. NM_052809.1.

UniGene

Rn.2589.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2B5H	X-ray	1.50	A	1-200	[»]
2GH2	X-ray	1.50	A	1-200	[»]
3ELN	X-ray	1.42	A	1-200	[»]

ProteinModelPortal

P21816.

SMR

P21816. Positions 4-190.

ModBase

Search...

Protein-protein interaction databases

STRING

10116.ENSRNOP00000000172.

Protocols and materials databases

DNASU

81718.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000000172; ENSRNOP00000000172; ENSRNOG00000000158.

GeneID

81718.

KEGG

rno:81718.

UCSC

RGD:69262. rat.

Organism-specific databases

CTD

1036.

RGD

69262. Cdo1.

Phylogenomic databases

eggNOG

NOG126313.

GeneTree

ENSGT00390000018226.

HOGENOM

HOG000177818.

HOVERGEN

HBG004469.

InParanoid

P21816.

K00456.

OMA

KVAYIND.

OrthoDB

EOG4W6NWZ.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-8844.

SABIO-RK

P21816.

UniPathway

UPA00012; UER00537.

Gene expression databases

Genevestigator

P21816.

GermOnline

ENSRNOG00000000158. Rattus norvegicus.

Family and domain databases

Gene3D

2.60.120.10. 1 hit.

InterPro

IPR010300. Cys_dOase_I.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]

Pfam

PF05995. CDO_I. 1 hit.
[Graphical view]

SUPFAM

SSF51182. RmlC_like_cupin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P21816.

NextBio

615363.

Entry information

Entry name

CDO1_RAT

Accession

Primary (citable) accession number: P21816
Secondary accession number(s): Q6NS32

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	April 3, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents