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P21816

- CDO1_RAT

UniProt

P21816 - CDO1_RAT

Protein

Cysteine dioxygenase type 1

Gene

Cdo1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-cysteine + O2 = 3-sulfinoalanine.

    Cofactori

    Binds 1 iron ion per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi86 – 861Iron; catalytic1 Publication
    Metal bindingi88 – 881Iron; catalytic1 Publication
    Metal bindingi140 – 1401Iron; catalytic1 Publication

    GO - Molecular functioni

    1. cysteine dioxygenase activity Source: UniProtKB
    2. ferrous iron binding Source: RGD

    GO - Biological processi

    1. cysteine metabolic process Source: RGD
    2. lactation Source: RGD
    3. L-cysteine catabolic process Source: RGD
    4. L-cysteine metabolic process Source: RGD
    5. response to amino acid Source: RGD
    6. response to cAMP Source: RGD
    7. response to ethanol Source: RGD
    8. response to glucagon Source: RGD
    9. response to glucocorticoid Source: RGD
    10. response to organonitrogen compound Source: RGD
    11. taurine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-8844.
    ReactomeiREACT_198615. Degradation of cysteine and homocysteine.
    SABIO-RKP21816.
    UniPathwayiUPA00012; UER00537.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine dioxygenase type 1 (EC:1.13.11.20)
    Alternative name(s):
    Cysteine dioxygenase type I
    Short name:
    CDO
    Short name:
    CDO-I
    Gene namesi
    Name:Cdo1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 18

    Organism-specific databases

    RGDi69262. Cdo1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 200200Cysteine dioxygenase type 1PRO_0000206609Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki93 ↔ 1573'-(S-cysteinyl)-tyrosine (Cys-Tyr)

    Post-translational modificationi

    The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe2+ ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.

    Keywords - PTMi

    Thioether bond

    Expressioni

    Tissue specificityi

    Highest levels in liver. Significant expression also in kidney, lung and brain.1 Publication

    Developmental stagei

    From neonate to weaning.

    Inductioni

    By increase of sulfur amino acid intake.

    Gene expression databases

    GenevestigatoriP21816.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000000172.

    Structurei

    Secondary structure

    1
    200
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi12 – 2211
    Beta strandi24 – 274
    Helixi30 – 3910
    Helixi44 – 474
    Helixi48 – 503
    Beta strandi55 – 573
    Beta strandi59 – 646
    Turni66 – 694
    Beta strandi71 – 777
    Beta strandi92 – 1009
    Beta strandi102 – 1076
    Beta strandi121 – 1255
    Beta strandi130 – 1334
    Turni135 – 1373
    Beta strandi139 – 1435
    Beta strandi151 – 1599
    Beta strandi162 – 1676
    Turni169 – 1713
    Beta strandi174 – 1785
    Beta strandi182 – 1843

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2B5HX-ray1.50A1-200[»]
    2GH2X-ray1.50A1-200[»]
    3ELNX-ray1.42A1-200[»]
    4IEOX-ray1.55A1-200[»]
    4IEPX-ray1.45A1-200[»]
    4IEQX-ray1.40A1-200[»]
    4IERX-ray1.45A1-200[»]
    4IESX-ray1.40A1-200[»]
    4IETX-ray1.40A1-200[»]
    4IEUX-ray1.25A1-200[»]
    4IEVX-ray1.60A1-200[»]
    4IEWX-ray1.45A1-200[»]
    4IEXX-ray2.15A1-200[»]
    4IEYX-ray1.63A1-200[»]
    4IEZX-ray1.39A1-200[»]
    4JTNX-ray1.59A1-200[»]
    4JTOX-ray2.00A1-200[»]
    4KWJX-ray1.75A1-200[»]
    4KWKX-ray1.95A1-200[»]
    4KWLX-ray1.63A1-200[»]
    ProteinModelPortaliP21816.
    SMRiP21816. Positions 5-190.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP21816.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cysteine dioxygenase family.Curated

    Phylogenomic databases

    eggNOGiNOG126313.
    GeneTreeiENSGT00390000018226.
    HOGENOMiHOG000177818.
    HOVERGENiHBG004469.
    InParanoidiP21816.
    KOiK00456.
    OMAiKMTFWSK.
    OrthoDBiEOG77M8PK.
    PhylomeDBiP21816.
    TreeFamiTF105636.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR010300. Cys_dOase_I.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PfamiPF05995. CDO_I. 1 hit.
    [Graphical view]
    SUPFAMiSSF51182. SSF51182. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P21816-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERTELLKPR TLADLIRILH ELFAGDEVNV EEVQAVLEAY ESNPAEWALY    50
    AKFDQYRYTR NLVDQGNGKF NLMILCWGEG HGSSIHDHTD SHCFLKLLQG 100
    NLKETLFDWP DKKSNEMIKK SERTLRENQC AYINDSIGLH RVENVSHTEP 150
    AVSLHLYSPP FDTCHAFDQR TGHKNKVTMT FHSKFGIRTP FTTSGSLENN 200
    Length:200
    Mass (Da):23,026
    Last modified:May 1, 1991 - v1
    Checksum:i118F1A3326B340F9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti89 – 891T → S in AAH70509. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35266 mRNA. Translation: AAA40904.1.
    D83481 Genomic DNA. Translation: BAA11925.1.
    BC070509 mRNA. Translation: AAH70509.1.
    PIRiA34632.
    RefSeqiNP_434696.1. NM_052809.1.
    UniGeneiRn.2589.

    Genome annotation databases

    EnsembliENSRNOT00000000172; ENSRNOP00000000172; ENSRNOG00000000158.
    GeneIDi81718.
    KEGGirno:81718.
    UCSCiRGD:69262. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35266 mRNA. Translation: AAA40904.1 .
    D83481 Genomic DNA. Translation: BAA11925.1 .
    BC070509 mRNA. Translation: AAH70509.1 .
    PIRi A34632.
    RefSeqi NP_434696.1. NM_052809.1.
    UniGenei Rn.2589.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2B5H X-ray 1.50 A 1-200 [» ]
    2GH2 X-ray 1.50 A 1-200 [» ]
    3ELN X-ray 1.42 A 1-200 [» ]
    4IEO X-ray 1.55 A 1-200 [» ]
    4IEP X-ray 1.45 A 1-200 [» ]
    4IEQ X-ray 1.40 A 1-200 [» ]
    4IER X-ray 1.45 A 1-200 [» ]
    4IES X-ray 1.40 A 1-200 [» ]
    4IET X-ray 1.40 A 1-200 [» ]
    4IEU X-ray 1.25 A 1-200 [» ]
    4IEV X-ray 1.60 A 1-200 [» ]
    4IEW X-ray 1.45 A 1-200 [» ]
    4IEX X-ray 2.15 A 1-200 [» ]
    4IEY X-ray 1.63 A 1-200 [» ]
    4IEZ X-ray 1.39 A 1-200 [» ]
    4JTN X-ray 1.59 A 1-200 [» ]
    4JTO X-ray 2.00 A 1-200 [» ]
    4KWJ X-ray 1.75 A 1-200 [» ]
    4KWK X-ray 1.95 A 1-200 [» ]
    4KWL X-ray 1.63 A 1-200 [» ]
    ProteinModelPortali P21816.
    SMRi P21816. Positions 5-190.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000000172.

    Protocols and materials databases

    DNASUi 81718.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000000172 ; ENSRNOP00000000172 ; ENSRNOG00000000158 .
    GeneIDi 81718.
    KEGGi rno:81718.
    UCSCi RGD:69262. rat.

    Organism-specific databases

    CTDi 1036.
    RGDi 69262. Cdo1.

    Phylogenomic databases

    eggNOGi NOG126313.
    GeneTreei ENSGT00390000018226.
    HOGENOMi HOG000177818.
    HOVERGENi HBG004469.
    InParanoidi P21816.
    KOi K00456.
    OMAi KMTFWSK.
    OrthoDBi EOG77M8PK.
    PhylomeDBi P21816.
    TreeFami TF105636.

    Enzyme and pathway databases

    UniPathwayi UPA00012 ; UER00537 .
    BioCyci MetaCyc:MONOMER-8844.
    Reactomei REACT_198615. Degradation of cysteine and homocysteine.
    SABIO-RK P21816.

    Miscellaneous databases

    EvolutionaryTracei P21816.
    NextBioi 615363.
    PROi P21816.

    Gene expression databases

    Genevestigatori P21816.

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    InterProi IPR010300. Cys_dOase_I.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view ]
    Pfami PF05995. CDO_I. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51182. SSF51182. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a cDNA for rat liver cysteine dioxygenase."
      Hosokawa Y., Matsumoto A., Oka J., Itakura H., Yamaguchi K.
      Biochem. Biophys. Res. Commun. 168:473-478(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Liver.
    2. "Structural organization and tissue-specific expression of the gene encoding rat cysteine dioxygenase."
      Tsuboyama N., Hosokawa Y., Totani M., Oka J., Matsumoto A., Koide T., Kodama H.
      Gene 181:161-165(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    4. "Crystal structure of mammalian cysteine dioxygenase. A novel mononuclear iron center for cysteine thiol oxidation."
      Simmons C.R., Liu Q., Huang Q., Hao Q., Begley T.P., Karplus P.A., Stipanuk M.H.
      J. Biol. Chem. 281:18723-18733(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH IRON, CROSS-LINK.

    Entry informationi

    Entry nameiCDO1_RAT
    AccessioniPrimary (citable) accession number: P21816
    Secondary accession number(s): Q6NS32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3