P21816 (CDO1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine dioxygenase type 1
EC=1.13.11.20

Alternative name(s):
Cysteine dioxygenase type I
Short name=CDO
Short name=CDO-I

Gene names

Name:

Cdo1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-cysteine + O2 = 3-sulfinoalanine.
Cofactor	Binds 1 iron ion per subunit.
Pathway	Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.
Subunit structure	Monomer.
Tissue specificity	Highest levels in liver. Significant expression also in kidney, lung and brain. Ref.2
Developmental stage	From neonate to weaning.
Induction	By increase of sulfur amino acid intake.
Post-translational modification	The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe2+ ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.
Sequence similarities	Belongs to the cysteine dioxygenase family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Phosphoprotein Thioether bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	L-cysteine catabolic process Inferred from direct assay. Source: RGD lactation Inferred from direct assay. Source: RGD response to amino acid stimulus Inferred from direct assay. Source: RGD response to cAMP Inferred from direct assay. Source: RGD response to ethanol Inferred from direct assay. Source: RGD response to glucagon stimulus Inferred from direct assay. Source: RGD response to glucocorticoid stimulus Inferred from direct assay. Source: RGD
Cellular component	cytosol Traceable author statement Ref.1. Source: RGD
Molecular function	cysteine dioxygenase activity Inferred from direct assay. Source: UniProtKB ferrous iron binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 200

200

Cysteine dioxygenase type 1

PRO_0000206609

Sites

Metal binding

86

1

Iron; catalytic

Metal binding

88

1

Iron; catalytic

Metal binding

140

1

Iron; catalytic

Amino acid modifications

Modified residue

59

1

Phosphothreonine By similarity

Cross-link

93 ↔ 157

3'-(S-cysteinyl)-tyrosine (Cys-Tyr)

Experimental info

Sequence conflict

89

1

T → S in AAH70509. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

200

Helix Strand Turn

Details...

Helix

12 – 22

11

Beta strand

24 – 27

4

Helix

30 – 39

10

Helix

44 – 47

4

Helix

48 – 50

3

Beta strand

55 – 57

3

Beta strand

59 – 64

6

Helix

66 – 68

3

Beta strand

71 – 77

7

Beta strand

92 – 100

9

Beta strand

102 – 107

6

Beta strand

121 – 125

5

Beta strand

130 – 133

4

Turn

135 – 137

3

Beta strand

139 – 143

5

Beta strand

151 – 159

9

Beta strand

162 – 167

6

Turn

169 – 171

3

Beta strand

174 – 178

5

Beta strand

182 – 184

3

Sequences

	Sequence		Length	Mass (Da)
	P21816 [UniParc]. Last modified May 1, 1991. Version 1. Checksum: 118F1A3326B340F9	FASTA	200	23,026
	10 20 30 40 50 60 MERTELLKPR TLADLIRILH ELFAGDEVNV EEVQAVLEAY ESNPAEWALY AKFDQYRYTR 70 80 90 100 110 120 NLVDQGNGKF NLMILCWGEG HGSSIHDHTD SHCFLKLLQG NLKETLFDWP DKKSNEMIKK 130 140 150 160 170 180 SERTLRENQC AYINDSIGLH RVENVSHTEP AVSLHLYSPP FDTCHAFDQR TGHKNKVTMT 190 200 FHSKFGIRTP FTTSGSLENN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a cDNA for rat liver cysteine dioxygenase." Hosokawa Y., Matsumoto A., Oka J., Itakura H., Yamaguchi K. Biochem. Biophys. Res. Commun. 168:473-478(1990) [PubMed: 2334417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Structural organization and tissue-specific expression of the gene encoding rat cysteine dioxygenase." Tsuboyama N., Hosokawa Y., Totani M., Oka J., Matsumoto A., Koide T., Kodama H. Gene 181:161-165(1996) [PubMed: 8973325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[4]	"Crystal structure of mammalian cysteine dioxygenase. A novel mononuclear iron center for cysteine thiol oxidation." Simmons C.R., Liu Q., Huang Q., Hao Q., Begley T.P., Karplus P.A., Stipanuk M.H. J. Biol. Chem. 281:18723-18733(2006) [PubMed: 16611640] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH IRON, CROSS-LINK.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M35266 mRNA. Translation: AAA40904.1.
D83481 Genomic DNA. Translation: BAA11925.1.
BC070509 mRNA. Translation: AAH70509.1.

IPI

IPI00331764.

PIR

A34632.

RefSeq

NP_434696.1. NM_052809.1.

UniGene

Rn.2589.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2B5H	X-ray	1.50	A	1-200	[»]
2GH2	X-ray	1.50	A	1-200	[»]
3ELN	X-ray	1.42	A	1-200	[»]

ProteinModelPortal

P21816.

SMR

P21816. Positions 4-190.

ModBase

Search...

Protein-protein interaction databases

STRING

P21816.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000000172; ENSRNOP00000000172; ENSRNOG00000000158.

GeneID

81718.

KEGG

rno:81718.

UCSC

NM_052809. rat.

Organism-specific databases

CTD

1036.

RGD

69262. Cdo1.

Phylogenomic databases

eggNOG

roNOG05170.

GeneTree

ENSGT00390000018226.

HOVERGEN

HBG004469.

InParanoid

P21816.

OMA

NDELGLH.

OrthoDB

EOG4W6NWZ.

PhylomeDB

P21816.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-8844.

Gene expression databases

Genevestigator

P21816.

GermOnline

ENSRNOG00000000158. Rattus norvegicus.

Family and domain databases

InterPro

IPR011051. Cupin_RmlC_type.
IPR010300. Cys_dOase_I.
IPR014710. RmlC-like_jellyroll.
[Graphical view]

Gene3D

G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.

KO

K00456.

Pfam

PF05995. CDO_I. 1 hit.
[Graphical view]

SUPFAM

SSF51182. RmlC_like_cupin. 1 hit.

ProtoNet

Search...

Other

NextBio

615363.

Entry information

Entry name

CDO1_RAT

Accession

Primary (citable) accession number: P21816
Secondary accession number(s): Q6NS32

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	November 16, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program