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P21816

- CDO1_RAT

UniProt

P21816 - CDO1_RAT

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Protein

Cysteine dioxygenase type 1

Gene

Cdo1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-cysteine + O2 = 3-sulfinoalanine.

Cofactori

Binds 1 iron ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861Iron; catalytic1 Publication
Metal bindingi88 – 881Iron; catalytic1 Publication
Metal bindingi140 – 1401Iron; catalytic1 Publication

GO - Molecular functioni

  1. cysteine dioxygenase activity Source: UniProtKB
  2. ferrous iron binding Source: RGD

GO - Biological processi

  1. cysteine metabolic process Source: RGD
  2. lactation Source: RGD
  3. L-cysteine catabolic process Source: RGD
  4. L-cysteine metabolic process Source: RGD
  5. response to amino acid Source: RGD
  6. response to cAMP Source: RGD
  7. response to ethanol Source: RGD
  8. response to glucagon Source: RGD
  9. response to glucocorticoid Source: RGD
  10. response to organonitrogen compound Source: RGD
  11. taurine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8844.
ReactomeiREACT_198615. Degradation of cysteine and homocysteine.
SABIO-RKP21816.
UniPathwayiUPA00012; UER00537.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine dioxygenase type 1 (EC:1.13.11.20)
Alternative name(s):
Cysteine dioxygenase type I
Short name:
CDO
Short name:
CDO-I
Gene namesi
Name:Cdo1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 18

Organism-specific databases

RGDi69262. Cdo1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Cysteine dioxygenase type 1PRO_0000206609Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki93 ↔ 1573'-(S-cysteinyl)-tyrosine (Cys-Tyr)

Post-translational modificationi

The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe2+ ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.

Keywords - PTMi

Thioether bond

Expressioni

Tissue specificityi

Highest levels in liver. Significant expression also in kidney, lung and brain.1 Publication

Developmental stagei

From neonate to weaning.

Inductioni

By increase of sulfur amino acid intake.

Gene expression databases

GenevestigatoriP21816.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000172.

Structurei

Secondary structure

1
200
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 2211
Beta strandi24 – 274
Helixi30 – 3910
Helixi44 – 474
Helixi48 – 503
Beta strandi55 – 573
Beta strandi59 – 646
Turni66 – 694
Beta strandi71 – 777
Beta strandi92 – 1009
Beta strandi102 – 1076
Beta strandi121 – 1255
Beta strandi130 – 1334
Turni135 – 1373
Beta strandi139 – 1435
Beta strandi151 – 1599
Beta strandi162 – 1676
Turni169 – 1713
Beta strandi174 – 1785
Beta strandi182 – 1843

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B5HX-ray1.50A1-200[»]
2GH2X-ray1.50A1-200[»]
3ELNX-ray1.42A1-200[»]
4IEOX-ray1.55A1-200[»]
4IEPX-ray1.45A1-200[»]
4IEQX-ray1.40A1-200[»]
4IERX-ray1.45A1-200[»]
4IESX-ray1.40A1-200[»]
4IETX-ray1.40A1-200[»]
4IEUX-ray1.25A1-200[»]
4IEVX-ray1.60A1-200[»]
4IEWX-ray1.45A1-200[»]
4IEXX-ray2.15A1-200[»]
4IEYX-ray1.63A1-200[»]
4IEZX-ray1.39A1-200[»]
4JTNX-ray1.59A1-200[»]
4JTOX-ray2.00A1-200[»]
4KWJX-ray1.75A1-200[»]
4KWKX-ray1.95A1-200[»]
4KWLX-ray1.63A1-200[»]
ProteinModelPortaliP21816.
SMRiP21816. Positions 5-190.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21816.

Family & Domainsi

Sequence similaritiesi

Belongs to the cysteine dioxygenase family.Curated

Phylogenomic databases

eggNOGiNOG126313.
GeneTreeiENSGT00390000018226.
HOGENOMiHOG000177818.
HOVERGENiHBG004469.
InParanoidiP21816.
KOiK00456.
OMAiKMTFWSK.
OrthoDBiEOG77M8PK.
PhylomeDBiP21816.
TreeFamiTF105636.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR010300. Cys_dOase_I.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF05995. CDO_I. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

P21816-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERTELLKPR TLADLIRILH ELFAGDEVNV EEVQAVLEAY ESNPAEWALY
60 70 80 90 100
AKFDQYRYTR NLVDQGNGKF NLMILCWGEG HGSSIHDHTD SHCFLKLLQG
110 120 130 140 150
NLKETLFDWP DKKSNEMIKK SERTLRENQC AYINDSIGLH RVENVSHTEP
160 170 180 190 200
AVSLHLYSPP FDTCHAFDQR TGHKNKVTMT FHSKFGIRTP FTTSGSLENN
Length:200
Mass (Da):23,026
Last modified:May 1, 1991 - v1
Checksum:i118F1A3326B340F9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891T → S in AAH70509. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35266 mRNA. Translation: AAA40904.1.
D83481 Genomic DNA. Translation: BAA11925.1.
BC070509 mRNA. Translation: AAH70509.1.
PIRiA34632.
RefSeqiNP_434696.1. NM_052809.1.
UniGeneiRn.2589.

Genome annotation databases

EnsembliENSRNOT00000000172; ENSRNOP00000000172; ENSRNOG00000000158.
GeneIDi81718.
KEGGirno:81718.
UCSCiRGD:69262. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35266 mRNA. Translation: AAA40904.1 .
D83481 Genomic DNA. Translation: BAA11925.1 .
BC070509 mRNA. Translation: AAH70509.1 .
PIRi A34632.
RefSeqi NP_434696.1. NM_052809.1.
UniGenei Rn.2589.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2B5H X-ray 1.50 A 1-200 [» ]
2GH2 X-ray 1.50 A 1-200 [» ]
3ELN X-ray 1.42 A 1-200 [» ]
4IEO X-ray 1.55 A 1-200 [» ]
4IEP X-ray 1.45 A 1-200 [» ]
4IEQ X-ray 1.40 A 1-200 [» ]
4IER X-ray 1.45 A 1-200 [» ]
4IES X-ray 1.40 A 1-200 [» ]
4IET X-ray 1.40 A 1-200 [» ]
4IEU X-ray 1.25 A 1-200 [» ]
4IEV X-ray 1.60 A 1-200 [» ]
4IEW X-ray 1.45 A 1-200 [» ]
4IEX X-ray 2.15 A 1-200 [» ]
4IEY X-ray 1.63 A 1-200 [» ]
4IEZ X-ray 1.39 A 1-200 [» ]
4JTN X-ray 1.59 A 1-200 [» ]
4JTO X-ray 2.00 A 1-200 [» ]
4KWJ X-ray 1.75 A 1-200 [» ]
4KWK X-ray 1.95 A 1-200 [» ]
4KWL X-ray 1.63 A 1-200 [» ]
ProteinModelPortali P21816.
SMRi P21816. Positions 5-190.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000000172.

Protocols and materials databases

DNASUi 81718.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000000172 ; ENSRNOP00000000172 ; ENSRNOG00000000158 .
GeneIDi 81718.
KEGGi rno:81718.
UCSCi RGD:69262. rat.

Organism-specific databases

CTDi 1036.
RGDi 69262. Cdo1.

Phylogenomic databases

eggNOGi NOG126313.
GeneTreei ENSGT00390000018226.
HOGENOMi HOG000177818.
HOVERGENi HBG004469.
InParanoidi P21816.
KOi K00456.
OMAi KMTFWSK.
OrthoDBi EOG77M8PK.
PhylomeDBi P21816.
TreeFami TF105636.

Enzyme and pathway databases

UniPathwayi UPA00012 ; UER00537 .
BioCyci MetaCyc:MONOMER-8844.
Reactomei REACT_198615. Degradation of cysteine and homocysteine.
SABIO-RK P21816.

Miscellaneous databases

EvolutionaryTracei P21816.
NextBioi 615363.
PROi P21816.

Gene expression databases

Genevestigatori P21816.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
InterProi IPR010300. Cys_dOase_I.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
Pfami PF05995. CDO_I. 1 hit.
[Graphical view ]
SUPFAMi SSF51182. SSF51182. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a cDNA for rat liver cysteine dioxygenase."
    Hosokawa Y., Matsumoto A., Oka J., Itakura H., Yamaguchi K.
    Biochem. Biophys. Res. Commun. 168:473-478(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  2. "Structural organization and tissue-specific expression of the gene encoding rat cysteine dioxygenase."
    Tsuboyama N., Hosokawa Y., Totani M., Oka J., Matsumoto A., Koide T., Kodama H.
    Gene 181:161-165(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  4. "Crystal structure of mammalian cysteine dioxygenase. A novel mononuclear iron center for cysteine thiol oxidation."
    Simmons C.R., Liu Q., Huang Q., Hao Q., Begley T.P., Karplus P.A., Stipanuk M.H.
    J. Biol. Chem. 281:18723-18733(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH IRON, CROSS-LINK.

Entry informationi

Entry nameiCDO1_RAT
AccessioniPrimary (citable) accession number: P21816
Secondary accession number(s): Q6NS32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: October 1, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3