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Protein

Cysteine dioxygenase type 1

Gene

Cdo1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-cysteine + O2 = 3-sulfinoalanine.

Cofactori

Fe cationNote: Binds 1 Fe cation per subunit.

Pathway: taurine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hypotaurine from L-cysteine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Cysteine dioxygenase type 1 (Cdo1)
  2. Cysteine sulfinic acid decarboxylase (Csad)
This subpathway is part of the pathway taurine biosynthesis, which is itself part of Organosulfur biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hypotaurine from L-cysteine, the pathway taurine biosynthesis and in Organosulfur biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861Iron; via tele nitrogen; catalytic1 Publication
Metal bindingi88 – 881Iron; via tele nitrogen; catalytic1 Publication
Metal bindingi140 – 1401Iron; via tele nitrogen; catalytic1 Publication

GO - Molecular functioni

  • cysteine dioxygenase activity Source: UniProtKB
  • ferrous iron binding Source: RGD

GO - Biological processi

  • cysteine metabolic process Source: RGD
  • lactation Source: RGD
  • L-cysteine catabolic process Source: RGD
  • L-cysteine metabolic process Source: RGD
  • response to amino acid Source: RGD
  • response to cAMP Source: RGD
  • response to ethanol Source: RGD
  • response to glucagon Source: RGD
  • response to glucocorticoid Source: RGD
  • response to organonitrogen compound Source: RGD
  • taurine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-8844.
BRENDAi1.13.11.20. 5301.
ReactomeiREACT_315719. Degradation of cysteine and homocysteine.
SABIO-RKP21816.
UniPathwayiUPA00012; UER00537.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine dioxygenase type 1 (EC:1.13.11.20)
Alternative name(s):
Cysteine dioxygenase type I
Short name:
CDO
Short name:
CDO-I
Gene namesi
Name:Cdo1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi69262. Cdo1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Cysteine dioxygenase type 1PRO_0000206609Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki93 ↔ 1573'-(S-cysteinyl)-tyrosine (Cys-Tyr)1 Publication

Post-translational modificationi

The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe2+ ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.1 Publication

Keywords - PTMi

Thioether bond

Expressioni

Tissue specificityi

Highest levels in liver. Significant expression also in kidney, lung and brain.1 Publication

Developmental stagei

From neonate to weaning.

Inductioni

By increase of sulfur amino acid intake.

Gene expression databases

GenevisibleiP21816. RN.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000172.

Structurei

Secondary structure

1
200
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 2211Combined sources
Beta strandi24 – 274Combined sources
Helixi30 – 3910Combined sources
Helixi44 – 474Combined sources
Helixi48 – 503Combined sources
Beta strandi55 – 573Combined sources
Beta strandi59 – 646Combined sources
Turni66 – 694Combined sources
Beta strandi71 – 777Combined sources
Beta strandi92 – 1009Combined sources
Beta strandi102 – 1076Combined sources
Beta strandi121 – 1255Combined sources
Beta strandi130 – 1334Combined sources
Turni135 – 1373Combined sources
Beta strandi139 – 1435Combined sources
Beta strandi151 – 1599Combined sources
Beta strandi162 – 1676Combined sources
Turni169 – 1713Combined sources
Beta strandi174 – 1785Combined sources
Beta strandi182 – 1843Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B5HX-ray1.50A1-200[»]
2GH2X-ray1.50A1-200[»]
3ELNX-ray1.42A1-200[»]
4IEOX-ray1.55A1-200[»]
4IEPX-ray1.45A1-200[»]
4IEQX-ray1.40A1-200[»]
4IERX-ray1.45A1-200[»]
4IESX-ray1.40A1-200[»]
4IETX-ray1.40A1-200[»]
4IEUX-ray1.25A1-200[»]
4IEVX-ray1.60A1-200[»]
4IEWX-ray1.45A1-200[»]
4IEXX-ray2.15A1-200[»]
4IEYX-ray1.63A1-200[»]
4IEZX-ray1.39A1-200[»]
4JTNX-ray1.59A1-200[»]
4JTOX-ray2.00A1-200[»]
4KWJX-ray1.75A1-200[»]
4KWKX-ray1.95A1-200[»]
4KWLX-ray1.63A1-200[»]
4UBGX-ray1.90A1-200[»]
4UBHX-ray1.81A1-200[»]
ProteinModelPortaliP21816.
SMRiP21816. Positions 5-190.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21816.

Family & Domainsi

Sequence similaritiesi

Belongs to the cysteine dioxygenase family.Curated

Phylogenomic databases

eggNOGiNOG126313.
GeneTreeiENSGT00390000018226.
HOGENOMiHOG000177818.
HOVERGENiHBG004469.
InParanoidiP21816.
KOiK00456.
OMAiCFMKLLQ.
OrthoDBiEOG77M8PK.
PhylomeDBiP21816.
TreeFamiTF105636.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR010300. Cys_dOase_I.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF05995. CDO_I. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

P21816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERTELLKPR TLADLIRILH ELFAGDEVNV EEVQAVLEAY ESNPAEWALY
60 70 80 90 100
AKFDQYRYTR NLVDQGNGKF NLMILCWGEG HGSSIHDHTD SHCFLKLLQG
110 120 130 140 150
NLKETLFDWP DKKSNEMIKK SERTLRENQC AYINDSIGLH RVENVSHTEP
160 170 180 190 200
AVSLHLYSPP FDTCHAFDQR TGHKNKVTMT FHSKFGIRTP FTTSGSLENN
Length:200
Mass (Da):23,026
Last modified:May 1, 1991 - v1
Checksum:i118F1A3326B340F9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891T → S in AAH70509 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35266 mRNA. Translation: AAA40904.1.
D83481 Genomic DNA. Translation: BAA11925.1.
BC070509 mRNA. Translation: AAH70509.1.
PIRiA34632.
RefSeqiNP_434696.1. NM_052809.1.
UniGeneiRn.2589.

Genome annotation databases

EnsembliENSRNOT00000000172; ENSRNOP00000000172; ENSRNOG00000000158.
GeneIDi81718.
KEGGirno:81718.
UCSCiRGD:69262. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35266 mRNA. Translation: AAA40904.1.
D83481 Genomic DNA. Translation: BAA11925.1.
BC070509 mRNA. Translation: AAH70509.1.
PIRiA34632.
RefSeqiNP_434696.1. NM_052809.1.
UniGeneiRn.2589.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2B5HX-ray1.50A1-200[»]
2GH2X-ray1.50A1-200[»]
3ELNX-ray1.42A1-200[»]
4IEOX-ray1.55A1-200[»]
4IEPX-ray1.45A1-200[»]
4IEQX-ray1.40A1-200[»]
4IERX-ray1.45A1-200[»]
4IESX-ray1.40A1-200[»]
4IETX-ray1.40A1-200[»]
4IEUX-ray1.25A1-200[»]
4IEVX-ray1.60A1-200[»]
4IEWX-ray1.45A1-200[»]
4IEXX-ray2.15A1-200[»]
4IEYX-ray1.63A1-200[»]
4IEZX-ray1.39A1-200[»]
4JTNX-ray1.59A1-200[»]
4JTOX-ray2.00A1-200[»]
4KWJX-ray1.75A1-200[»]
4KWKX-ray1.95A1-200[»]
4KWLX-ray1.63A1-200[»]
4UBGX-ray1.90A1-200[»]
4UBHX-ray1.81A1-200[»]
ProteinModelPortaliP21816.
SMRiP21816. Positions 5-190.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000172.

Protocols and materials databases

DNASUi81718.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000172; ENSRNOP00000000172; ENSRNOG00000000158.
GeneIDi81718.
KEGGirno:81718.
UCSCiRGD:69262. rat.

Organism-specific databases

CTDi1036.
RGDi69262. Cdo1.

Phylogenomic databases

eggNOGiNOG126313.
GeneTreeiENSGT00390000018226.
HOGENOMiHOG000177818.
HOVERGENiHBG004469.
InParanoidiP21816.
KOiK00456.
OMAiCFMKLLQ.
OrthoDBiEOG77M8PK.
PhylomeDBiP21816.
TreeFamiTF105636.

Enzyme and pathway databases

UniPathwayiUPA00012; UER00537.
BioCyciMetaCyc:MONOMER-8844.
BRENDAi1.13.11.20. 5301.
ReactomeiREACT_315719. Degradation of cysteine and homocysteine.
SABIO-RKP21816.

Miscellaneous databases

EvolutionaryTraceiP21816.
NextBioi615363.
PROiP21816.

Gene expression databases

GenevisibleiP21816. RN.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR010300. Cys_dOase_I.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF05995. CDO_I. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a cDNA for rat liver cysteine dioxygenase."
    Hosokawa Y., Matsumoto A., Oka J., Itakura H., Yamaguchi K.
    Biochem. Biophys. Res. Commun. 168:473-478(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  2. "Structural organization and tissue-specific expression of the gene encoding rat cysteine dioxygenase."
    Tsuboyama N., Hosokawa Y., Totani M., Oka J., Matsumoto A., Koide T., Kodama H.
    Gene 181:161-165(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  4. "Crystal structure of mammalian cysteine dioxygenase. A novel mononuclear iron center for cysteine thiol oxidation."
    Simmons C.R., Liu Q., Huang Q., Hao Q., Begley T.P., Karplus P.A., Stipanuk M.H.
    J. Biol. Chem. 281:18723-18733(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH IRON, CROSS-LINK.

Entry informationi

Entry nameiCDO1_RAT
AccessioniPrimary (citable) accession number: P21816
Secondary accession number(s): Q6NS32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: June 24, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.