Cysteine dioxygenase type 1 - Rattus norvegicus (Rat)

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Reviewed, UniProtKB/Swiss-Prot P21816 (CDO1_RAT)

Last modified June 16, 2009. Version 71. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Cysteine dioxygenase type 1
    EC=1.13.11.20
Alternative name(s):
    Cysteine dioxygenase type I
      Short name=CDO-I
      Short name=CDO

Gene names

Name:

Cdo1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	L-cysteine + O₂ = 3-sulfinoalanine.
Cofactor	Binds 1 iron ion per subunit.
Pathway	Organosulfur biosynthesis; taurine biosynthesis; hypotaurine from L-cysteine: step 1/2.
Subunit structure	Monomer.
Tissue specificity	Highest levels in liver. Significant expression also in kidney, lung and brain. Ref.2
Developmental stage	From neonate to weaning.
Induction	By increase of sulfur amino acid intake.
Post-translational modification	The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe²⁺ ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.
Sequence similarities	Belongs to the cysteine dioxygenase family.

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Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
PTM	Phosphoprotein Thioether bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	L-cysteine catabolic process Inferred from direct assay. Source: RGD lactation Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to amino acid stimulus Inferred from direct assay. Source: RGD response to cAMP Inferred from direct assay. Source: RGD response to ethanol Inferred from direct assay. Source: RGD response to glucagon stimulus Inferred from direct assay. Source: RGD response to glucocorticoid stimulus Inferred from direct assay. Source: RGD
Cellular component	cytosol Ref.1 Traceable author statement. Source: RGD
Molecular function	cysteine dioxygenase activity Ref.1 Inferred from direct assay. Source: UniProtKB ferrous iron binding Inferred from direct assay. Source: RGD
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 200

200

Cysteine dioxygenase type 1

PRO_0000206609

Sites

Metal binding

Iron; catalytic

Metal binding

Iron; catalytic

Metal binding

140

Iron; catalytic

Amino acid modifications

Modified residue

Phosphothreonine By similarity

Cross-link

93 ↔ 157

3'-(S-cysteinyl)-tyrosine (Cys-Tyr)

Experimental info

Sequence conflict

T → S in AAH70509. Ref.3

Secondary structure

200

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P21816-1 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: 118F1A3326B340F9
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FASTA

200

23,026

        10         20         30         40         50         60 
MERTELLKPR TLADLIRILH ELFAGDEVNV EEVQAVLEAY ESNPAEWALY AKFDQYRYTR 

        70         80         90        100        110        120 
NLVDQGNGKF NLMILCWGEG HGSSIHDHTD SHCFLKLLQG NLKETLFDWP DKKSNEMIKK 

       130        140        150        160        170        180 
SERTLRENQC AYINDSIGLH RVENVSHTEP AVSLHLYSPP FDTCHAFDQR TGHKNKVTMT 

       190        200 
FHSKFGIRTP FTTSGSLENN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a cDNA for rat liver cysteine dioxygenase." Hosokawa Y., Matsumoto A., Oka J., Itakura H., Yamaguchi K. Biochem. Biophys. Res. Commun. 168:473-478(1990) [PubMed: 2334417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Structural organization and tissue-specific expression of the gene encoding rat cysteine dioxygenase." Tsuboyama N., Hosokawa Y., Totani M., Oka J., Matsumoto A., Koide T., Kodama H. Gene 181:161-165(1996) [PubMed: 8973325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[4]	"Crystal structure of mammalian cysteine dioxygenase. A novel mononuclear iron center for cysteine thiol oxidation." Simmons C.R., Liu Q., Huang Q., Hao Q., Begley T.P., Karplus P.A., Stipanuk M.H. J. Biol. Chem. 281:18723-18733(2006) [PubMed: 16611640] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH IRON, CROSS-LINK.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M35266 mRNA. Translation: AAA40904.1.
D83481 Genomic DNA. Translation: BAA11925.1.
BC070509 mRNA. Translation: AAH70509.1.

IPI

IPI00331764.

PIR

A34632.

RefSeq

NP_434696.1.

UniGene

Rn.2589

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2B5H	X-ray	1.50	A	1-200	[»]
2GH2	X-ray	1.50	A	1-200	[»]
3ELN	X-ray	1.42	A	1-200	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSRNOG00000000158. Rattus norvegicus. [Contig view]

GeneID

81718.

KEGG

rno:81718.

Organism-specific databases

RGD

69262. Cdo1.

Phylogenomic databases

HOVERGEN

P21816.

OMA

P21816. EWAAYAK.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-8844.

BRENDA

1.13.11.20. 248.

Gene expression databases

ArrayExpress

P21816.

GermOnline

ENSRNOG00000000158. Rattus norvegicus.

Family and domain databases

InterPro

IPR010300. Cys_dOase_I.
[Graphical view]

Pfam

PF05995. CDO_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

615363.

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Entry information

Entry name

CDO1_RAT

Accession

Primary (citable) accession number: P21816
Secondary accession number(s): Q6NS32

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	May 1, 1991
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families