P21815 (SIAL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bone sialoprotein 2

Alternative name(s):
Bone sialoprotein II
Short name=BSP II
Cell-binding sialoprotein
Integrin-binding sialoprotein

Gene names

Name:	IBSP
Synonyms:	BNSP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
Subcellular location	Secreted.
Post-translational modification	N-glycosylated; glycans consist of sialylated and core-fucosylated bi-, tri- and tetraantennary chains. Ref.6 O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc. Ref.6 Sulfated on either Tyr-313 or Tyr-314.
Miscellaneous	It is possible that the segments of clustered carboxyl groups mediate the strong binding to hydroxyapatite.

Ontologies

Keywords
Biological process	Biomineralization Cell adhesion
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Ligand	Sialic acid
PTM	Glycoprotein Phosphoprotein Sulfation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	biomineral tissue development Inferred from electronic annotation. Source: UniProtKB-KW cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW cellular response to growth factor stimulus Inferred from electronic annotation. Source: Compara extracellular matrix organization Inferred from electronic annotation. Source: Compara ossification Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16
	Chain	17 – 317	301	Bone sialoprotein 2		PRO_0000020330
Regions
	Motif	286 – 288	3	Cell attachment site
	Compositional bias	65 – 173	109	Asp/Glu-rich (acidic)
	Compositional bias	76 – 83	8	Poly-Glu
	Compositional bias	151 – 158	8	Poly-Glu
Amino acid modifications
	Modified residue	31	1	Phosphoserine Ref.7
	Modified residue	67	1	Phosphoserine By similarity
	Modified residue	74	1	Phosphoserine By similarity
	Modified residue	75	1	Phosphoserine By similarity
	Modified residue	94	1	Phosphoserine By similarity
	Modified residue	100	1	Phosphoserine By similarity
	Modified residue	149	1	Phosphoserine By similarity
	Modified residue	280	1	Phosphoserine By similarity
	Modified residue	313	1	Sulfotyrosine Ref.7
	Modified residue	314	1	Sulfotyrosine Ref.7
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	O-linked (GalNAc...) Probable
	Glycosylation	122	1	O-linked (GalNAc...) Probable
	Glycosylation	177	1	N-linked (GlcNAc...) Potential
	Glycosylation	182	1	N-linked (GlcNAc...) Potential
	Glycosylation	190	1	N-linked (GlcNAc...) Potential
	Glycosylation	227	1	O-linked (GalNAc...) Probable
	Glycosylation	228	1	O-linked (GalNAc...) Probable
	Glycosylation	229	1	O-linked (GalNAc...) Probable
	Glycosylation	238	1	O-linked (GalNAc...) Probable
	Glycosylation	239	1	O-linked (GalNAc...) Probable
Natural variations
	Natural variant	195	1	G → E. Ref.2 Ref.3 Corresponds to variant rs1054627 [ dbSNP | Ensembl ].		VAR_058014
	Natural variant	213	1	D → G. Ref.1 Ref.2 Ref.3 Ref.6 Corresponds to variant rs13144371 [ dbSNP | Ensembl ].		VAR_058015
	Natural variant	219	1	R → G. Ref.1 Ref.2 Corresponds to variant rs17013181 [ dbSNP | Ensembl ].		VAR_058016
	Natural variant	256	1	T → A. Corresponds to variant rs17013182 [ dbSNP | Ensembl ].		VAR_056579
	Natural variant	268	1	A → V. Ref.1 Corresponds to variant rs1054628 [ dbSNP | Ensembl ].		VAR_056580
	Natural variant	270	1	E → D. Ref.2 Ref.3 Corresponds to variant rs1054629 [ dbSNP | Ensembl ].		VAR_058017
Experimental info
	Sequence conflict	94	1	S → L in AAC37560. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P21815 [UniParc]. Last modified January 11, 2011. Version 4. Checksum: 736CB6B8C3716FE7	FASTA	317	35,148
	10 20 30 40 50 60 MKTALILLSI LGMACAFSMK NLHRRVKIED SEENGVFKYR PRYYLYKHAY FYPHLKRFPV 70 80 90 100 110 120 QGSSDSSEEN GDDSSEEEEE EEETSNEGEN NEESNEDEDS EAENTTLSAT TLGYGEDATP 130 140 150 160 170 180 GTGYTGLAAI QLPKKAGDIT NKATKEKESD EEEEEEEEGN ENEESEAEVD ENEQGINGTS 190 200 210 220 230 240 TNSTEAENGN GSSGGDNGEE GEEESVTGAN AEDTTETGRQ GKGTSKTTTS PNGGFEPTTP 250 260 270 280 290 300 PQVYRTTSPP FGKTTTVEYE GEYEYTGANE YDNGYEIYES ENGEPRGDNY RAYEDEYSYF 310 KGQGYDGYDG QNYYHHQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human bone sialoprotein. Deduced protein sequence and chromosomal localization." Fisher L.W., McBride O.W., Termine J.D., Young M.F. J. Biol. Chem. 265:2347-2351(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-213; GLY-219 AND VAL-268.
[2]	"The human bone sialoprotein gene (IBSP): genomic localization and characterization." Kerr J.M., Fisher L.W., Termine J.D., Wang M.G., McBride W., Young M.F. Genomics 17:408-415(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLU-195; GLY-213; GLY-219 AND ASP-270.
[3]	"Characterization of the human bone sialoprotein (BSP) gene and its promoter sequence." Kim R.H., Shapiro H.S., Li J.J., Wrana J.L., Sodek J. Matrix Biol. 14:31-40(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLU-195; GLY-213 AND ASP-270.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Purification and partial characterization of small proteoglycans I and II, bone sialoproteins I and II, and osteonectin from the mineral compartment of developing human bone." Fisher L.W., Hawkins G.R., Tuross N., Termine J.D. J. Biol. Chem. 262:9702-9708(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
[6]	"Structural characterization of human recombinant and bone-derived bone sialoprotein. Functional implications for cell attachment and hydroxyapatite binding." Wuttke M., Muller S., Nitsche D.P., Paulsson M., Hanisch F.G., Maurer P. J. Biol. Chem. 276:36839-36848(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, GLYCOSYLATION AT THR-119; THR-122; THR-227; THR-228; THR-229; THR-238 AND THR-239, STRUCTURE OF CARBOHYDRATE, MASS SPECTROMETRY, VARIANT GLY-213.
[7]	"Posttranslational modifications to human bone sialoprotein determined by mass spectrometry." Zaia J., Boynton R., Heinegard D., Barry F. Biochemistry 40:12983-12991(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-31, SULFATION AT TYR-313 AND TYR-314, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05213 mRNA. Translation: AAC95490.1. L09558 , L09554, L09555, L09556, L09557 Genomic DNA. Translation: AAA60549.1. L24759, L24757 Genomic DNA. Translation: AAC37560.1. AC093768 Genomic DNA. No translation available.
IPI	IPI00294662.
PIR	GEHUS. A35043.
RefSeq	NP_004958.2. NM_004967.3.
UniGene	Hs.518726.
3D structure databases
DisProt	DP00332.
ProteinModelPortal	P21815.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000226284.
PTM databases
PhosphoSite	P21815.
Polymorphism databases
DMDM	251757441.
Proteomic databases
PaxDb	P21815.
PRIDE	P21815.
Protocols and materials databases
DNASU	3381.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000226284; ENSP00000226284; ENSG00000029559.
GeneID	3381.
KEGG	hsa:3381.
UCSC	uc003hqx.4. human.
Organism-specific databases
CTD	3381.
GeneCards	GC04P088720.
H-InvDB	HIX0031506.
HGNC	HGNC:5341. IBSP.
MIM	147563. gene.
neXtProt	NX_P21815.
PharmGKB	PA29590.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG238366.
HOGENOM	HOG000234824.
HOVERGEN	HBG007981.
InParanoid	P21815.
KO	K06253.
OMA	AYEDEYS.
OrthoDB	EOG4FTW1Z.
PhylomeDB	P21815.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P21815.
CleanEx	HS_IBSP.
Genevestigator	P21815.
GermOnline	ENSG00000029559. Homo sapiens.
Family and domain databases
InterPro	IPR008412. BSP_II. [Graphical view]
PANTHER	PTHR10345. PTHR10345. 1 hit.
Pfam	PF05432. BSP_II. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	3381.
NextBio	13366.
SOURCE	Search...

Entry information

Entry name

SIAL_HUMAN

Accession

Primary (citable) accession number: P21815

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	January 11, 2011
Last modified:	April 3, 2013
This is version 109 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.