Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mast cell protease 4

Gene

Mcpt4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-|-Ile-5' bond of angiotensin I and the 'Phe-20-|-Ala-21' bond of amyloid beta-protein, and is less active towards the 'Phe-8-|-His-9' bond of angiotensin I and the 'Phe-4-|-Ala-5' and 'Tyr-10-|-Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing.2 Publications

Enzyme regulationi

Completely inhibited by serine protease inhibitors such as chymostatin, diisopropylfluorophosphate and phenylmethylsulfonyl fluoride, but not by p-tosyl-L-phenylalanine chloromethyl ketone, p-tosyl-L-lysine chloromethyl ketone, pepstatin, E-64, EDTA or o-phenanthroline. Also inhibited by lima bean trypsin inhibitor, soy bean trypsin inhibitor and human plasma alpha1-antichymotrypsin.

pH dependencei

Optimum pH is 9 at high salt concentrations.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay systemBy similarity1
Active sitei109Charge relay systemBy similarity1
Active sitei202Charge relay systemBy similarity1

GO - Molecular functioni

  • peptidase activity Source: MGI
  • serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • immune response Source: GO_Central
  • protein processing Source: GO_Central
  • proteolysis Source: UniProtKB
  • regulation of angiotensin levels in blood Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.149.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast cell protease 4 (EC:3.4.21.-)
Short name:
mMCP-4
Alternative name(s):
MSMCP
Myonase
Serosal mast cell protease
Gene namesi
Name:Mcpt4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96940. Mcpt4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • intracellular Source: MGI
  • intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mice display an impaired ability to inactivate thrombin or degrade fibronectin in peritoneal cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Add BLAST18
PropeptideiPRO_000002745319 – 20Activation peptide3 Publications2
ChainiPRO_000002745421 – 246Mast cell protease 4Add BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Disulfide bondi143 ↔ 208PROSITE-ProRule annotation
Disulfide bondi174 ↔ 187PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

MaxQBiP21812.
PaxDbiP21812.
PeptideAtlasiP21812.
PRIDEiP21812.

PTM databases

iPTMnetiP21812.
PhosphoSitePlusiP21812.

Expressioni

Tissue specificityi

Submucosal mast cells. In femoral muscle, detected in myocytes but not in mast cells.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038103.

Structurei

3D structure databases

ProteinModelPortaliP21812.
SMRiP21812.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 244Peptidase S1PROSITE-ProRule annotationAdd BLAST224

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP21812.
PhylomeDBiP21812.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQALLFLMAL LLPSGAGAEE IIGGVESRPH SRPYMAHLEI TTERGFTATC
60 70 80 90 100
GGFLITRQFV MTAAHCSGRE ITVTLGAHDV SKTESTQQKI KVEKQIVHPK
110 120 130 140 150
YNFYSNLHDI MLLKLQKKAK ETPSVNVIPL PRPSDFIKPG KMCRAAGWGR
160 170 180 190 200
TGVTEPTSDT LREVKLRIMD KEACKNYWHY DYNLQVCVGS PRKKRSAYKG
210 220 230 240
DSGGPLLCAG VAHGIVSYGR GDAKPPAVFT RISSYVPWIN RVIKGE
Length:246
Mass (Da):27,204
Last modified:May 1, 1991 - v1
Checksum:i0887A1C71ACE2698
GO

Sequence cautioni

The sequence BAB18732 differs from that shown. Reason: Erroneous initiation.Curated

Mass spectrometryi

Molecular mass is 25187 Da from positions 21 - 246. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti61M → L in strain: C57BL/6 and Leaden X A1. 1
Natural varianti160T → I in strain: C57BL/6 and Leaden X A1. 1
Natural varianti246E → KK in strain: C57BL/6 and Leaden X A1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55617 mRNA. Translation: AAA39989.1.
M55616 Genomic DNA. Translation: AAA72939.1.
AY007569 mRNA. Translation: AAG24503.1.
X68804 mRNA. Translation: CAA48704.1.
AB051900 mRNA. Translation: BAB18732.1. Different initiation.
CCDSiCCDS27140.1.
PIRiB38678.
JE0151.
S26042.
RefSeqiNP_034909.2. NM_010779.2.
UniGeneiMm.439684.

Genome annotation databases

GeneIDi17227.
KEGGimmu:17227.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55617 mRNA. Translation: AAA39989.1.
M55616 Genomic DNA. Translation: AAA72939.1.
AY007569 mRNA. Translation: AAG24503.1.
X68804 mRNA. Translation: CAA48704.1.
AB051900 mRNA. Translation: BAB18732.1. Different initiation.
CCDSiCCDS27140.1.
PIRiB38678.
JE0151.
S26042.
RefSeqiNP_034909.2. NM_010779.2.
UniGeneiMm.439684.

3D structure databases

ProteinModelPortaliP21812.
SMRiP21812.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038103.

Protein family/group databases

MEROPSiS01.149.

PTM databases

iPTMnetiP21812.
PhosphoSitePlusiP21812.

Proteomic databases

MaxQBiP21812.
PaxDbiP21812.
PeptideAtlasiP21812.
PRIDEiP21812.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17227.
KEGGimmu:17227.

Organism-specific databases

CTDi17227.
MGIiMGI:96940. Mcpt4.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP21812.
PhylomeDBiP21812.

Miscellaneous databases

PROiP21812.
SOURCEiSearch...

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCPT4_MOUSE
AccessioniPrimary (citable) accession number: P21812
Secondary accession number(s): Q9EPQ9, Q9EQT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.