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P21812

- MCPT4_MOUSE

UniProt

P21812 - MCPT4_MOUSE

Protein

Mast cell protease 4

Gene

Mcpt4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 May 1991)
      Previous versions | rss
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    Functioni

    Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-|-Ile-5' bond of angiotensin I and the 'Phe-20-|-Ala-21' bond of amyloid beta-protein, and is less active towards the 'Phe-8-|-His-9' bond of angiotensin I and the 'Phe-4-|-Ala-5' and 'Tyr-10-|-Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing.2 Publications

    Enzyme regulationi

    Completely inhibited by serine protease inhibitors such as chymostatin, diisopropylfluorophosphate and phenylmethylsulfonyl fluoride, but not by p-tosyl-L-phenylalanine chloromethyl ketone, p-tosyl-L-lysine chloromethyl ketone, pepstatin, E-64, EDTA or o-phenanthroline. Also inhibited by lima bean trypsin inhibitor, soy bean trypsin inhibitor and human plasma alpha1-antichymotrypsin.

    pH dependencei

    Optimum pH is 9 at high salt concentrations.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Charge relay systemBy similarity
    Active sitei109 – 1091Charge relay systemBy similarity
    Active sitei202 – 2021Charge relay systemBy similarity

    GO - Molecular functioni

    1. peptidase activity Source: MGI
    2. serine-type endopeptidase activity Source: UniProtKB

    GO - Biological processi

    1. proteolysis Source: UniProtKB
    2. regulation of angiotensin levels in blood Source: MGI

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.149.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mast cell protease 4 (EC:3.4.21.-)
    Short name:
    mMCP-4
    Alternative name(s):
    MSMCP
    Myonase
    Serosal mast cell protease
    Gene namesi
    Name:Mcpt4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:96940. Mcpt4.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: MGI
    2. secretory granule Source: RefGenome

    Pathology & Biotechi

    Disruption phenotypei

    Mice display an impaired ability to inactivate thrombin or degrade fibronectin in peritoneal cells.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Add
    BLAST
    Propeptidei19 – 202Activation peptide3 PublicationsPRO_0000027453
    Chaini21 – 246226Mast cell protease 4PRO_0000027454Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
    Disulfide bondi143 ↔ 208PROSITE-ProRule annotation
    Disulfide bondi174 ↔ 187PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    MaxQBiP21812.
    PRIDEiP21812.

    Expressioni

    Tissue specificityi

    Submucosal mast cells. In femoral muscle, detected in myocytes but not in mast cells.1 Publication

    Gene expression databases

    GenevestigatoriP21812.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP21812.
    SMRiP21812. Positions 21-243.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 244224Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP21812.
    KOiK01362.
    PhylomeDBiP21812.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21812-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQALLFLMAL LLPSGAGAEE IIGGVESRPH SRPYMAHLEI TTERGFTATC    50
    GGFLITRQFV MTAAHCSGRE ITVTLGAHDV SKTESTQQKI KVEKQIVHPK 100
    YNFYSNLHDI MLLKLQKKAK ETPSVNVIPL PRPSDFIKPG KMCRAAGWGR 150
    TGVTEPTSDT LREVKLRIMD KEACKNYWHY DYNLQVCVGS PRKKRSAYKG 200
    DSGGPLLCAG VAHGIVSYGR GDAKPPAVFT RISSYVPWIN RVIKGE 246
    Length:246
    Mass (Da):27,204
    Last modified:May 1, 1991 - v1
    Checksum:i0887A1C71ACE2698
    GO

    Sequence cautioni

    The sequence BAB18732.1 differs from that shown. Reason: Erroneous initiation.

    Mass spectrometryi

    Molecular mass is 25187 Da from positions 21 - 246. Determined by MALDI. 1 Publication

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti61 – 611M → L in strain: C57BL/6 and Leaden X A1.
    Natural varianti160 – 1601T → I in strain: C57BL/6 and Leaden X A1.
    Natural varianti246 – 2461E → KK in strain: C57BL/6 and Leaden X A1.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55617 mRNA. Translation: AAA39989.1.
    M55616 Genomic DNA. Translation: AAA72939.1.
    AY007569 mRNA. Translation: AAG24503.1.
    X68804 mRNA. Translation: CAA48704.1.
    AB051900 mRNA. Translation: BAB18732.1. Different initiation.
    CCDSiCCDS27140.1.
    PIRiB38678.
    JE0151.
    S26042.
    RefSeqiNP_034909.2. NM_010779.2.
    UniGeneiMm.439684.

    Genome annotation databases

    GeneIDi17227.
    KEGGimmu:17227.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55617 mRNA. Translation: AAA39989.1 .
    M55616 Genomic DNA. Translation: AAA72939.1 .
    AY007569 mRNA. Translation: AAG24503.1 .
    X68804 mRNA. Translation: CAA48704.1 .
    AB051900 mRNA. Translation: BAB18732.1 . Different initiation.
    CCDSi CCDS27140.1.
    PIRi B38678.
    JE0151.
    S26042.
    RefSeqi NP_034909.2. NM_010779.2.
    UniGenei Mm.439684.

    3D structure databases

    ProteinModelPortali P21812.
    SMRi P21812. Positions 21-243.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.149.

    Proteomic databases

    MaxQBi P21812.
    PRIDEi P21812.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 17227.
    KEGGi mmu:17227.

    Organism-specific databases

    CTDi 17227.
    MGIi MGI:96940. Mcpt4.

    Phylogenomic databases

    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P21812.
    KOi K01362.
    PhylomeDBi P21812.

    Miscellaneous databases

    NextBioi 291644.
    PROi P21812.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P21812.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the cDNA and gene for mouse mast cell protease 4. Demonstration of its late transcription in mast cell subclasses and analysis of its homology to subclass-specific neutral proteases of the mouse and rat."
      Serafin W.E., Sullivan T.P., Conder G.A., Ebrahimi A., Marcham P., Johnson S.S., Austen K.F., Reynolds D.S.
      J. Biol. Chem. 266:1934-1941(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: BALB/c.
    2. "Independent influence of strain difference and mi transcription factor on the expression of mouse mast cell chymases."
      Ge Y., Jippo T., Lee Y.-M., Adachi S., Kitamura Y.
      Am. J. Pathol. 158:281-292(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    3. "Cloning and structural analysis of MMCP-1, MMCP-4 and MMCP-5, three mouse mast cell-specific serine proteases."
      Huang R., Blom T., Hellman L.
      Eur. J. Immunol. 21:1611-1621(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 7-246.
      Strain: Leaden X A1.
      Tissue: Mastocytoma.
    4. "Purification and characterization of myonase from X-chromosome linked muscular dystrophic mouse skeletal muscle."
      Hori S., Ohtani S., Hori C., Nokihara K.
      J. Biochem. 123:650-658(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 7-246, PROTEIN SEQUENCE OF 21-66; 76-84; 86-93; 96-106; 126-143; 146-155; 178-180; 197-207 AND 231-238, FUNCTION, SUBUNIT, TISSUE SPECIFICITY, MASS SPECTROMETRY.
      Tissue: Skeletal muscle.
    5. "Different mouse mast cell populations express various combinations of at least six distinct mast cell serine proteases."
      Reynolds D.S., Stevens R.L., Lane W.S., Carr M.H., Austen K.F., Serafin W.E.
      Proc. Natl. Acad. Sci. U.S.A. 87:3230-3234(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-43.
    6. "Biochemical and immunological characterization of multiple glycoforms of mouse mast cell protease 1: comparison with an isolated murine serosal mast cell protease (MMCP-4)."
      Newlands G.F.J., Knox D.P., Pirie-Shepherd S.R., Miller H.R.P.
      Biochem. J. 294:127-135(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-40.
    7. "The chymase, mouse mast cell protease 4, constitutes the major chymotrypsin-like activity in peritoneum and ear tissue. A role for mouse mast cell protease 4 in thrombin regulation and fibronectin turnover."
      Tchougounova E., Pejler G., Abrink M.
      J. Exp. Med. 198:423-431(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiMCPT4_MOUSE
    AccessioniPrimary (citable) accession number: P21812
    Secondary accession number(s): Q9EPQ9, Q9EQT2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: May 1, 1991
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3