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Protein

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

Gene

SDH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDH1 and SDH2 form the catalytic dimer. Electrons flow from succinate to the FAD bound to SDH1, and sequentially through the iron-sulfur clusters bound to SDH2 and enter the membrane dimer formed by SDH3 and SDH4.

Miscellaneous

Present with 9540 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Succinate + a quinone = fumarate + a quinol.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes fumarate from succinate (eukaryal route).
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate dehydrogenase [ubiquinone] flavoprotein subunit 2, mitochondrial (YJL045W), Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDH2), Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDH1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes fumarate from succinate (eukaryal route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi87Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi92Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi95Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi107Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi179Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi182Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi185Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi189Iron-sulfur 3 (3Fe-4S)By similarity1
Binding sitei194Ubiquinone; shared with DHSDBy similarity1
Metal bindingi236Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi242Iron-sulfur 3 (3Fe-4S)By similarity1
Metal bindingi246Iron-sulfur 2 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular respiration Source: SGD
  • respiratory electron transport chain Source: GO_Central
  • tricarboxylic acid cycle Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport, Tricarboxylic acid cycle
Ligand2Fe-2S, 3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:YLL041C-MONOMER.
YEAST:YLL041C-MONOMER.
UniPathwayiUPA00223; UER01006.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (EC:1.3.5.1)
Alternative name(s):
Iron-sulfur subunit of complex II
Short name:
Ip
Gene namesi
Name:SDH2
Synonyms:SDH, SDHB
Ordered Locus Names:YLL041C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLL041C.
SGDiS000003964. SDH2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi260 – 266Missing : Abolishes SDH activity and complex assembly. 1 Publication7
Mutagenesisi260K → T: Abolishes SDH activity. No effect on complex assembly and stability; when associated with T-261. 1 Publication1
Mutagenesisi261K → T: Abolishes SDH activity. No effect on complex assembly and stability; when associated with T-260. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – ?20MitochondrionAdd BLAST20
ChainiPRO_0000010353?21 – 266Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrialAdd BLAST246

Proteomic databases

MaxQBiP21801.
PRIDEiP21801.

Interactioni

Subunit structurei

Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit.By similarity

Protein-protein interaction databases

BioGridi31272. 140 interactors.
DIPiDIP-5856N.
IntActiP21801. 7 interactors.
MINTiMINT-695166.
STRINGi4932.YLL041C.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ORZmodel-B21-266[»]
1PB4model-B21-266[»]
ProteinModelPortaliP21801.
SMRiP21801.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 1272Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST92
Domaini169 – 1994Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST31

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000013558.
HOGENOMiHOG000160590.
InParanoidiP21801.
KOiK00235.
OMAiDGQYFGP.
OrthoDBiEOG092C401C.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiView protein in InterPro
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012675. Beta-grasp_dom.
IPR009051. Helical_ferredxn.
IPR004489. Succ_DH/fum_Rdtase_Fe-S.
IPR025192. Succ_DH/fum_Rdtase_N.
PfamiView protein in Pfam
PF13085. Fer2_3. 1 hit.
SUPFAMiSSF46548. SSF46548. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR00384. dhsB. 1 hit.
PROSITEiView protein in PROSITE
PS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21801-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNVLLRRKA FCLVTKKGMA TATTAAATHT PRLKTFKVYR WNPDEPSAKP
60 70 80 90 100
HLQSYQVDLN DCGPMVLDAL LKIKDEQDST LTFRRSCREG ICGSCAMNIG
110 120 130 140 150
GRNTLACICK IDQNESKQLK IYPLPHMFIV KDLVPDLTNF YQQYKSIQPY
160 170 180 190 200
LQRSSFPKDG TEVLQSIEDR KKLDGLYECI LCACCSTSCP SYWWNQEQYL
210 220 230 240 250
GPAVLMQAYR WLIDSRDQAT KTRKAMLNNS MSLYRCHTIM NCTRTCPKGL
260
NPGLAIAEIK KSLAFA
Length:266
Mass (Da):30,231
Last modified:May 1, 1991 - v1
Checksum:iC14170F3670F6930
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05487 Genomic DNA. Translation: AAA35021.1.
Z73146 Genomic DNA. Translation: CAA97492.1.
AY558189 Genomic DNA. Translation: AAS56515.1.
BK006945 Genomic DNA. Translation: DAA09281.1.
PIRiA35435. RDBYIS.
RefSeqiNP_013059.1. NM_001181861.1.

Genome annotation databases

EnsemblFungiiYLL041C; YLL041C; YLL041C.
GeneIDi850685.
KEGGisce:YLL041C.

Similar proteinsi

Entry informationi

Entry nameiSDHB_YEAST
AccessioniPrimary (citable) accession number: P21801
Secondary accession number(s): D6VXW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: September 27, 2017
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names