P21778 (K6PF2_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: 6-phosphofructokinase isozyme 2 Short name=PFK2 Short name=Phosphofructokinase 2 EC=2.7.1.11 Alternative name(s): Phosphohexokinase 2
Organism	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifier	300852 [NCBI]
Taxonomic lineage	Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	25 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Enzyme regulation	In contrast with PFK1 this enzyme is not affected by phosphoenolpyruvate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm HAMAP MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›25	›25	6-phosphofructokinase isozyme 2 HAMAP MF_00339		PRO_0000112002
Regions
	Nucleotide binding	21 – 25	5	ATP By similarity
Experimental info
	Non-terminal residue	25	1

Sequences

	Sequence		Length	Mass (Da)
	P21778 [UniParc]. Last modified April 12, 2005. Version 3. Checksum: E7AEC8D6110EBA46	FASTA	25	2,557
	10 20 MKRIGVLTSG GDSPGMNAAI RAVVR

References

[1]	"Phosphoenolpyruvate-insensitive phosphofructokinase isozyme from Thermus thermophilus HB8." Xu J., Oshima T., Yoshida M. J. Biochem. 109:199-203(1991) [PubMed: 1830879] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P21778.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase. [Tree]
InterPro	IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF2_THET8

Accession

Primary (citable) accession number: P21778

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	April 12, 2005
Last modified:	October 5, 2010
This is version 68 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program