Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P21778 (PFKA2_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase 2

Short name=ATP-PFK 2
Short name=Phosphofructokinase 2
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase 2
Gene names
Name:pfkA2
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length25 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

Cofactor

Magnesium By similarity.

Enzyme regulation

In contrast with PFK1 this enzyme is not affected by phosphoenolpyruvate. Ref.1

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Subunit structure

Homotetramer. Ref.1

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termAllosteric enzyme
Direct protein sequencing
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›25›25ATP-dependent 6-phosphofructokinase 2
PRO_0000112002

Sites

Binding site111ATP; via amide nitrogen By similarity

Experimental info

Non-terminal residue251

Sequences

Sequence LengthMass (Da)Tools
P21778 [UniParc].

Last modified April 12, 2005. Version 3.
Checksum: E7AEC8D6110EBA46

FASTA252,557
        10         20 
MKRIGVLTSG GDSPGMNAAI RAVVR 

« Hide

References

[1]"Phosphoenolpyruvate-insensitive phosphofructokinase isozyme from Thermus thermophilus HB8."
Xu J., Oshima T., Yoshida M.
J. Biochem. 109:199-203(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, ENZYME REGULATION, SUBUNIT.

Cross-references

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00182.

Family and domain databases

InterProIPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
SUPFAMSSF53784. SSF53784. 1 hit.
ProtoNetSearch...

Entry information

Entry namePFKA2_THET8
AccessionPrimary (citable) accession number: P21778
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: April 12, 2005
Last modified: July 9, 2014
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways