Reviewed, UniProtKB/Swiss-Prot P21777 (K6PF1_THET8)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase isozyme 1
      Short name=Phosphofructokinase 1
      Short name=PFK1
    EC=2.7.1.11
Alternative name(s):
    Phosphohexokinase 1

Gene names

Name:	pfkA
Ordered Locus Names:	TTHA1962

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Enzyme regulation	Allosterically inhibited by phosphoenolpyruvate which induces the dissociation of the active tetramer into an inactive two-subunit forms. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subunit structure	Homotetramer. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	6-phosphofructokinase isozyme 1 HAMAP MF_00339		PRO_0000112001
Regions
	Nucleotide binding	21 – 25	5	ATP By similarity
	Nucleotide binding	155 – 159	5	ATP By similarity
	Nucleotide binding	172 – 188	17	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Metal binding	186	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	188	1	Magnesium By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity
Experimental info
	Sequence conflict	111	1	C → F in AAA27501. Ref.1
	Sequence conflict	273	1	A → P in AAA27501. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P21777-1 [UniParc]. Last modified March 29, 2005. Version 3. Checksum: 608CD4053E6ACEA3	FASTA	322	33,537
	10 20 30 40 50 60 MKRIGVFTSG GDAPGMNAAI RAVVRQAHAL GVEVIGIRRG YAGMIQGEMV PLGVRDVANI 70 80 90 100 110 120 IQRGGTILLT ARSQEFLTEE GRAKAYAKLQ AAGIEGLVAI GGDGTFRGAL CLVEEHGMPV 130 140 150 160 170 180 VGVPGTIDND LYGTDYTIGF DTAVNTALEA IDRIRDTAAS HERVFFIEVM GRHAGFIALD 190 200 210 220 230 240 VGLAGGAEVI AVPEEPVDPK AVAEVLEASQ RRGKKSSIVV VAEGAYPGGA AGLLAAIREH 250 260 270 280 290 300 LQVEARVTVL GHIQRGGSPT AKDRILASRL GAAAVEALVG GASGVMVGEV EGEVDLTPLK 310 320 EAVERRKDIN RALLRLSQVL AL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of phosphofructokinase 1 gene from a thermophilic bacterium, Thermus thermophilus." Xu J., Seki M., Denda K., Yoshida M. Biochem. Biophys. Res. Commun. 176:1313-1318(1991) [PubMed: 1828151] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Phosphoenolpyruvate-insensitive phosphofructokinase isozyme from Thermus thermophilus HB8." Xu J., Oshima T., Yoshida M. J. Biochem. 109:199-203(1991) [PubMed: 1830879] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M71213 Genomic DNA. Translation: AAA27501.1. AP008226 Genomic DNA. Translation: BAD71785.1.
RefSeq	YP_145228.1.
3D structure databases
HSSP	HSSP built from PDB template 6PFK based on UniProtKB P00512.
ModBase	Search...
Genome annotation databases
GeneID	3169721.
GenomeReviews	Gene locus TTHA1962 in contig AP008226_GR.
KEGG	ttj:TTHA1962.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P21777.
OMA	P21777. DTCLNTV.
Enzyme and pathway databases
BioCyc	TTHE300852:TTHA1962-MON.
Family and domain databases
HAMAP	MF_00339. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_THET8

Accession

Primary (citable) accession number: P21777
Secondary accession number(s): Q5SGW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	March 29, 2005
Last modified:	June 16, 2009
This is version 69 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)