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P21777 (K6PF1_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphofructokinase isozyme 1

Short name=PFK1
Short name=Phosphofructokinase 1
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase 1
Gene names
Name:pfkA
Ordered Locus Names:TTHA1962
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length322 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically inhibited by phosphoenolpyruvate which induces the dissociation of the active tetramer into an inactive two-subunit forms. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3223226-phosphofructokinase isozyme 1 HAMAP-Rule MF_00339
PRO_0000112001

Regions

Nucleotide binding21 – 255ATP By similarity
Nucleotide binding155 – 1595ATP By similarity
Nucleotide binding172 – 18817ATP By similarity

Sites

Active site1281Proton acceptor By similarity
Metal binding1861Magnesium; via carbonyl oxygen By similarity
Metal binding1881Magnesium By similarity
Binding site1631Substrate By similarity
Binding site2461Substrate By similarity
Binding site2521Substrate By similarity
Binding site2551Substrate By similarity

Experimental info

Sequence conflict1111C → F in AAA27501. Ref.1
Sequence conflict2731A → P in AAA27501. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P21777 [UniParc].

Last modified March 29, 2005. Version 3.
Checksum: 608CD4053E6ACEA3

FASTA32233,537
        10         20         30         40         50         60 
MKRIGVFTSG GDAPGMNAAI RAVVRQAHAL GVEVIGIRRG YAGMIQGEMV PLGVRDVANI 

        70         80         90        100        110        120 
IQRGGTILLT ARSQEFLTEE GRAKAYAKLQ AAGIEGLVAI GGDGTFRGAL CLVEEHGMPV 

       130        140        150        160        170        180 
VGVPGTIDND LYGTDYTIGF DTAVNTALEA IDRIRDTAAS HERVFFIEVM GRHAGFIALD 

       190        200        210        220        230        240 
VGLAGGAEVI AVPEEPVDPK AVAEVLEASQ RRGKKSSIVV VAEGAYPGGA AGLLAAIREH 

       250        260        270        280        290        300 
LQVEARVTVL GHIQRGGSPT AKDRILASRL GAAAVEALVG GASGVMVGEV EGEVDLTPLK 

       310        320 
EAVERRKDIN RALLRLSQVL AL 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of phosphofructokinase 1 gene from a thermophilic bacterium, Thermus thermophilus."
Xu J., Seki M., Denda K., Yoshida M.
Biochem. Biophys. Res. Commun. 176:1313-1318(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[3]"Phosphoenolpyruvate-insensitive phosphofructokinase isozyme from Thermus thermophilus HB8."
Xu J., Oshima T., Yoshida M.
J. Biochem. 109:199-203(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-25.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M71213 Genomic DNA. Translation: AAA27501.1.
AP008226 Genomic DNA. Translation: BAD71785.1.
RefSeqYP_145228.1. NC_006461.1.

3D structure databases

ProteinModelPortalP21777.
SMRP21777. Positions 1-321.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1962.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71785; BAD71785; BAD71785.
GeneID3169721.
KEGGttj:TTHA1962.
PATRIC23958903. VBITheThe93045_1933.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248869.
KOK00850.
OMARRGYQGM.
OrthoDBEOG644ZRM.
ProtClustDBPRK03202.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-2012-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK6PF1_THET8
AccessionPrimary (citable) accession number: P21777
Secondary accession number(s): Q5SGW8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: March 29, 2005
Last modified: April 16, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways