P21777 (K6PF1_THET8) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 88.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 6-phosphofructokinase isozyme 1 Short name=PFK1 Short name=Phosphofructokinase 1 EC=2.7.1.11 Alternative name(s): Phosphohexokinase 1 | ||||
| Gene names |
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| Organism | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | ||||
| Taxonomic identifier | 300852 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus |
Protein attributes
| Sequence length | 322 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339 |
| Enzyme regulation | Allosterically inhibited by phosphoenolpyruvate which induces the dissociation of the active tetramer into an inactive two-subunit forms. HAMAP MF_00339 |
| Pathway | Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339 |
| Subunit structure | Homotetramer. |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00339. |
| Sequence similarities | Belongs to the phosphofructokinase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Kinase Transferase |
| Technical term | Allosteric enzyme Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysisInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | 6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro |
| Molecular function | 6-phosphofructokinase activity Inferred from electronic annotation. Source: EC ATP bindingInferred from electronic annotation. Source: UniProtKB-KW metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 322 | 322 | 6-phosphofructokinase isozyme 1 HAMAP MF_00339 | PRO_0000112001 | |||||
Regions | |||||||||
| Nucleotide binding | 21 – 25 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 155 – 159 | 5 | ATP By similarity | ||||||
| Nucleotide binding | 172 – 188 | 17 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 128 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 186 | 1 | Magnesium; via carbonyl oxygen By similarity | ||||||
| Metal binding | 188 | 1 | Magnesium By similarity | ||||||
| Binding site | 163 | 1 | Substrate By similarity | ||||||
| Binding site | 246 | 1 | Substrate By similarity | ||||||
| Binding site | 252 | 1 | Substrate By similarity | ||||||
| Binding site | 255 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 111 | 1 | C → F in AAA27501. Ref.1 | ||||||
| Sequence conflict | 273 | 1 | A → P in AAA27501. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning of phosphofructokinase 1 gene from a thermophilic bacterium, Thermus thermophilus." Xu J., Seki M., Denda K., Yoshida M. Biochem. Biophys. Res. Commun. 176:1313-1318(1991) [PubMed: 1828151] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579. |
| [3] | "Phosphoenolpyruvate-insensitive phosphofructokinase isozyme from Thermus thermophilus HB8." Xu J., Oshima T., Yoshida M. J. Biochem. 109:199-203(1991) [PubMed: 1830879] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M71213 Genomic DNA. Translation: AAA27501.1. AP008226 Genomic DNA. Translation: BAD71785.1. |
| RefSeq | YP_145228.1. NC_006461.1. |
3D structure databases | |
| ProteinModelPortal | P21777. |
| SMR | P21777. Positions 1-321. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P21777. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 3169721. |
| GenomeReviews | Gene locus TTHA1962 in contig AP008226_GR. |
| KEGG | ttj:TTHA1962. |
| PATRIC | 23958903. VBITheThe93045_1933. |
Phylogenomic databases | |
| eggNOG | COG0205. |
| HOGENOM | HBG405044. |
| OMA | MVHHDII. |
| PhylomeDB | P21777. |
| ProtClustDB | PRK03202. |
Enzyme and pathway databases | |
| BioCyc | TTHE300852:TTHA1962-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00339. Phosphofructokinase. [Tree] |
| InterPro | IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view] |
| KO | K00850. |
| Pfam | PF00365. PFK. 1 hit. [Graphical view] |
| PIRSF | PIRSF000532. ATP_PFK_prok. 1 hit. |
| PRINTS | PR00476. PHFRCTKINASE. |
| SUPFAM | SSF53784. Ppfruckinase. 1 hit. |
| TIGRFAMs | TIGR02482. PFKA_ATP. 1 hit. |
| PROSITE | PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | K6PF1_THET8 | ||||||||
| Accession | Primary (citable) accession number: P21777 Secondary accession number(s): Q5SGW8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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