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P21775

- THIKA_RAT

UniProt

P21775 - THIKA_RAT

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Protein

3-ketoacyl-CoA thiolase A, peroxisomal

Gene

Acaa1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei123 – 1231Acyl-thioester intermediateBy similarity
Active sitei377 – 3771Proton acceptorPROSITE-ProRule annotation
Active sitei408 – 4081Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. acetyl-CoA C-acyltransferase activity Source: UniProtKB-EC
  2. palmitoyl-CoA oxidase activity Source: UniProtKB

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB
  2. response to drug Source: RGD
  3. response to nutrient Source: RGD
  4. response to steroid hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

SABIO-RKP21775.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolase A, peroxisomal (EC:2.3.1.16)
Alternative name(s):
Acetyl-CoA acyltransferase A
Beta-ketothiolase A
Peroxisomal 3-oxoacyl-CoA thiolase A
Gene namesi
Name:Acaa1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi67379. Acaa1a.

Subcellular locationi

GO - Cellular componenti

  1. peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626Peroxisome1 PublicationAdd
BLAST
Chaini27 – 4243983-ketoacyl-CoA thiolase A, peroxisomalPRO_0000034070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei173 – 1731N6-acetyllysineBy similarity
Modified residuei234 – 2341N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP21775.
PRIDEiP21775.

PTM databases

PhosphoSiteiP21775.

Expressioni

Inductioni

Peroxisomal thiolase is markedly induced (at the level of transcription) by various hypolipidemic compounds in parallel with the other two enzymes of the peroxisomal beta-oxidation system.

Gene expression databases

GenevestigatoriP21775.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041776.

Structurei

3D structure databases

ProteinModelPortaliP21775.
SMRiP21775. Positions 31-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0183.
HOGENOMiHOG000012239.
HOVERGENiHBG003112.
InParanoidiP21775.
KOiK07513.
PhylomeDBiP21775.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P21775-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHRLQVVLGH LAGRPESSSA LQAAPCSATF PQASASDVVV VHGRRTPIGR
60 70 80 90 100
AGRGGFKDTT PDELLSAVLT AVLQDVKLKP ECLGDISVGN VLEPGAGAVM
110 120 130 140 150
ARIAQFLSGI PETVPLSAVN RQCSSGLQAV ANIAGGIRNG SYDIGMACGV
160 170 180 190 200
ESMSLSNRGN PGNISSRLLE SDKARDCLIP MGITSENVAE RFGISRQKQD
210 220 230 240 250
AFALASQQKA ASAQSKGCFR AEIVPVTTTV LDDKGDRKTI TVSQDEGVRP
260 270 280 290 300
STTMEGLAKL KPAFKDGGST TAGNSSQVSD GAAAVLLARR SKAEELGLPI
310 320 330 340 350
LGVLRSYAVV GVPPDIMGIG PAYAIPAALQ KAGLTVNDID IFEINEAFAS
360 370 380 390 400
QALYCVEKLG IPAEKVNPLG GAIALGHPLG CTGARQVVTL LNELKRRGRR
410 420
AYGVVSMCIG TGMGAAAVFE YPGN
Length:424
Mass (Da):43,833
Last modified:August 30, 2005 - v2
Checksum:iD987625F3FF94F1C
GO
Isoform 2 (identifier: P21775-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     334-424: LTVNDIDIFE...AAAVFEYPGN → PLLCGEAGNS...RSKAGGHAAQ

Note: No experimental confirmation available.

Show »
Length:373
Mass (Da):38,118
Checksum:i4DA3FD8B35A6403B
GO

Sequence cautioni

The sequence AAA41471.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH89821.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAA14106.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291T → S in AAH89821. (PubMed:15489334)Curated
Sequence conflicti235 – 2351G → S in AAH89821. (PubMed:15489334)Curated
Sequence conflicti399 – 3991R → T in AAA41471. (PubMed:2210380)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei334 – 42491LTVND…EYPGN → PLLCGEAGNSCREGEPPGGC NSPGPPPGLHRSKAGGHAAQ in isoform 2. 1 PublicationVSP_023746Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M32801 mRNA. Translation: AAA41471.1. Different initiation.
D90058 Genomic DNA. Translation: BAA14106.1. Different initiation.
BC089821 mRNA. Translation: AAH89821.1. Different initiation.
PIRiA35725. XURTAA.
RefSeqiNP_036621.1. NM_012489.2.
UniGeneiRn.8913.

Genome annotation databases

GeneIDi24157.
KEGGirno:24157.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M32801 mRNA. Translation: AAA41471.1 . Different initiation.
D90058 Genomic DNA. Translation: BAA14106.1 . Different initiation.
BC089821 mRNA. Translation: AAH89821.1 . Different initiation.
PIRi A35725. XURTAA.
RefSeqi NP_036621.1. NM_012489.2.
UniGenei Rn.8913.

3D structure databases

ProteinModelPortali P21775.
SMRi P21775. Positions 31-424.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000041776.

PTM databases

PhosphoSitei P21775.

Proteomic databases

PaxDbi P21775.
PRIDEi P21775.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24157.
KEGGi rno:24157.

Organism-specific databases

CTDi 113868.
RGDi 67379. Acaa1a.

Phylogenomic databases

eggNOGi COG0183.
HOGENOMi HOG000012239.
HOVERGENi HBG003112.
InParanoidi P21775.
KOi K07513.
PhylomeDBi P21775.

Enzyme and pathway databases

UniPathwayi UPA00199 .
SABIO-RK P21775.

Miscellaneous databases

NextBioi 602445.
PROi P21775.

Gene expression databases

Genevestigatori P21775.

Family and domain databases

Gene3Di 3.40.47.10. 4 hits.
InterProi IPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view ]
Pfami PF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMi SSF53901. SSF53901. 2 hits.
TIGRFAMsi TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEi PS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence determination of cDNA encoding a second rat liver peroxisomal 3-ketoacyl-CoA thiolase."
    Bodnar A.G., Rachubinski R.A.
    Gene 91:193-199(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
  2. "Rat peroxisomal 3-ketoacyl-CoA thiolase gene. Occurrence of two closely related but differentially regulated genes."
    Hijikata M., Wen J.K., Osumi T., Hashimoto T.
    J. Biol. Chem. 265:4600-4606(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Lung.
  4. "A novel, cleavable peroxisomal targeting signal at the amino-terminus of the rat 3-ketoacyl-CoA thiolase."
    Swinkels B.W., Gould S.J., Bodnar A.G., Rachubinski R.A., Subramani S.
    EMBO J. 10:3255-3262(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSIT PEPTIDE CLEAVAGE SITE.

Entry informationi

Entry nameiTHIKA_RAT
AccessioniPrimary (citable) accession number: P21775
Secondary accession number(s): Q5FVR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: August 30, 2005
Last modified: October 1, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3