Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-ketoacyl-CoA thiolase A, peroxisomal

Gene

Acaa1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

Pathway: fatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei123 – 1231Acyl-thioester intermediateBy similarity
Active sitei377 – 3771Proton acceptorPROSITE-ProRule annotation
Active sitei408 – 4081Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  • acetyl-CoA C-acyltransferase activity Source: UniProtKB-EC
  • palmitoyl-CoA oxidase activity Source: UniProtKB

GO - Biological processi

  • fatty acid beta-oxidation Source: UniProtKB
  • response to drug Source: RGD
  • response to nutrient Source: RGD
  • response to steroid hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

SABIO-RKP21775.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolase A, peroxisomal (EC:2.3.1.16)
Alternative name(s):
Acetyl-CoA acyltransferase A
Beta-ketothiolase A
Peroxisomal 3-oxoacyl-CoA thiolase A
Gene namesi
Name:Acaa1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi67379. Acaa1a.

Subcellular locationi

GO - Cellular componenti

  • peroxisome Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626Peroxisome1 PublicationAdd
BLAST
Chaini27 – 4243983-ketoacyl-CoA thiolase A, peroxisomalPRO_0000034070Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei173 – 1731N6-acetyllysineBy similarity
Modified residuei234 – 2341N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP21775.
PRIDEiP21775.

PTM databases

PhosphoSiteiP21775.

Expressioni

Inductioni

Peroxisomal thiolase is markedly induced (at the level of transcription) by various hypolipidemic compounds in parallel with the other two enzymes of the peroxisomal beta-oxidation system.

Gene expression databases

GenevisibleiP21775. RN.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000050691.

Structurei

3D structure databases

ProteinModelPortaliP21775.
SMRiP21775. Positions 31-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0183.
HOGENOMiHOG000012239.
HOVERGENiHBG003112.
InParanoidiP21775.
KOiK07513.
PhylomeDBiP21775.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P21775-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHRLQVVLGH LAGRPESSSA LQAAPCSATF PQASASDVVV VHGRRTPIGR
60 70 80 90 100
AGRGGFKDTT PDELLSAVLT AVLQDVKLKP ECLGDISVGN VLEPGAGAVM
110 120 130 140 150
ARIAQFLSGI PETVPLSAVN RQCSSGLQAV ANIAGGIRNG SYDIGMACGV
160 170 180 190 200
ESMSLSNRGN PGNISSRLLE SDKARDCLIP MGITSENVAE RFGISRQKQD
210 220 230 240 250
AFALASQQKA ASAQSKGCFR AEIVPVTTTV LDDKGDRKTI TVSQDEGVRP
260 270 280 290 300
STTMEGLAKL KPAFKDGGST TAGNSSQVSD GAAAVLLARR SKAEELGLPI
310 320 330 340 350
LGVLRSYAVV GVPPDIMGIG PAYAIPAALQ KAGLTVNDID IFEINEAFAS
360 370 380 390 400
QALYCVEKLG IPAEKVNPLG GAIALGHPLG CTGARQVVTL LNELKRRGRR
410 420
AYGVVSMCIG TGMGAAAVFE YPGN
Length:424
Mass (Da):43,833
Last modified:August 30, 2005 - v2
Checksum:iD987625F3FF94F1C
GO
Isoform 2 (identifier: P21775-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     334-424: LTVNDIDIFE...AAAVFEYPGN → PLLCGEAGNS...RSKAGGHAAQ

Note: No experimental confirmation available.
Show »
Length:373
Mass (Da):38,118
Checksum:i4DA3FD8B35A6403B
GO

Sequence cautioni

The sequence AAA41471.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH89821.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA14106.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291T → S in AAH89821 (PubMed:15489334).Curated
Sequence conflicti235 – 2351G → S in AAH89821 (PubMed:15489334).Curated
Sequence conflicti399 – 3991R → T in AAA41471 (PubMed:2210380).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei334 – 42491LTVND…EYPGN → PLLCGEAGNSCREGEPPGGC NSPGPPPGLHRSKAGGHAAQ in isoform 2. 1 PublicationVSP_023746Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32801 mRNA. Translation: AAA41471.1. Different initiation.
D90058 Genomic DNA. Translation: BAA14106.1. Different initiation.
BC089821 mRNA. Translation: AAH89821.1. Different initiation.
PIRiA35725. XURTAA.
RefSeqiNP_036621.1. NM_012489.2.
UniGeneiRn.8913.

Genome annotation databases

GeneIDi24157.
KEGGirno:24157.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32801 mRNA. Translation: AAA41471.1. Different initiation.
D90058 Genomic DNA. Translation: BAA14106.1. Different initiation.
BC089821 mRNA. Translation: AAH89821.1. Different initiation.
PIRiA35725. XURTAA.
RefSeqiNP_036621.1. NM_012489.2.
UniGeneiRn.8913.

3D structure databases

ProteinModelPortaliP21775.
SMRiP21775. Positions 31-424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000050691.

PTM databases

PhosphoSiteiP21775.

Proteomic databases

PaxDbiP21775.
PRIDEiP21775.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24157.
KEGGirno:24157.

Organism-specific databases

CTDi113868.
RGDi67379. Acaa1a.

Phylogenomic databases

eggNOGiCOG0183.
HOGENOMiHOG000012239.
HOVERGENiHBG003112.
InParanoidiP21775.
KOiK07513.
PhylomeDBiP21775.

Enzyme and pathway databases

UniPathwayiUPA00199.
SABIO-RKP21775.

Miscellaneous databases

NextBioi602445.
PROiP21775.

Gene expression databases

GenevisibleiP21775. RN.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence determination of cDNA encoding a second rat liver peroxisomal 3-ketoacyl-CoA thiolase."
    Bodnar A.G., Rachubinski R.A.
    Gene 91:193-199(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: Sprague-Dawley.
  2. "Rat peroxisomal 3-ketoacyl-CoA thiolase gene. Occurrence of two closely related but differentially regulated genes."
    Hijikata M., Wen J.K., Osumi T., Hashimoto T.
    J. Biol. Chem. 265:4600-4606(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Lung.
  4. "A novel, cleavable peroxisomal targeting signal at the amino-terminus of the rat 3-ketoacyl-CoA thiolase."
    Swinkels B.W., Gould S.J., Bodnar A.G., Rachubinski R.A., Subramani S.
    EMBO J. 10:3255-3262(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TRANSIT PEPTIDE CLEAVAGE SITE.

Entry informationi

Entry nameiTHIKA_RAT
AccessioniPrimary (citable) accession number: P21775
Secondary accession number(s): Q5FVR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: August 30, 2005
Last modified: June 24, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.