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P21775 (THIKA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-ketoacyl-CoA thiolase A, peroxisomal

EC=2.3.1.16
Alternative name(s):
Acetyl-CoA acyltransferase A
Beta-ketothiolase A
Peroxisomal 3-oxoacyl-CoA thiolase A
Gene names
Name:Acaa1a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length424 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

Pathway

Lipid metabolism; fatty acid metabolism.

Subunit structure

Homodimer.

Subcellular location

Peroxisome.

Induction

Peroxisomal thiolase is markedly induced (at the level of transcription) by various hypolipidemic compounds in parallel with the other two enzymes of the peroxisomal beta-oxidation system.

Sequence similarities

Belongs to the thiolase family.

Sequence caution

The sequence AAA41471.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH89821.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA14106.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P21775-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P21775-2)

The sequence of this isoform differs from the canonical sequence as follows:
     334-424: LTVNDIDIFE...AAAVFEYPGN → PLLCGEAGNS...RSKAGGHAAQ
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2626Peroxisome
Chain27 – 4243983-ketoacyl-CoA thiolase A, peroxisomal
PRO_0000034070

Sites

Active site1231Acyl-thioester intermediate By similarity
Active site3771Proton acceptor By similarity
Active site4081Proton acceptor By similarity

Amino acid modifications

Modified residue1731N6-acetyllysine By similarity
Modified residue2341N6-acetyllysine By similarity

Natural variations

Alternative sequence334 – 42491LTVND…EYPGN → PLLCGEAGNSCREGEPPGGC NSPGPPPGLHRSKAGGHAAQ in isoform 2.
VSP_023746

Experimental info

Sequence conflict291T → S in AAH89821. Ref.3
Sequence conflict2351G → S in AAH89821. Ref.3
Sequence conflict3991R → T in AAA41471. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: D987625F3FF94F1C

FASTA42443,833
        10         20         30         40         50         60 
MHRLQVVLGH LAGRPESSSA LQAAPCSATF PQASASDVVV VHGRRTPIGR AGRGGFKDTT 

        70         80         90        100        110        120 
PDELLSAVLT AVLQDVKLKP ECLGDISVGN VLEPGAGAVM ARIAQFLSGI PETVPLSAVN 

       130        140        150        160        170        180 
RQCSSGLQAV ANIAGGIRNG SYDIGMACGV ESMSLSNRGN PGNISSRLLE SDKARDCLIP 

       190        200        210        220        230        240 
MGITSENVAE RFGISRQKQD AFALASQQKA ASAQSKGCFR AEIVPVTTTV LDDKGDRKTI 

       250        260        270        280        290        300 
TVSQDEGVRP STTMEGLAKL KPAFKDGGST TAGNSSQVSD GAAAVLLARR SKAEELGLPI 

       310        320        330        340        350        360 
LGVLRSYAVV GVPPDIMGIG PAYAIPAALQ KAGLTVNDID IFEINEAFAS QALYCVEKLG 

       370        380        390        400        410        420 
IPAEKVNPLG GAIALGHPLG CTGARQVVTL LNELKRRGRR AYGVVSMCIG TGMGAAAVFE 


YPGN 

« Hide

Isoform 2 [UniParc].

Checksum: 4DA3FD8B35A6403B
Show »

FASTA37338,118

References

« Hide 'large scale' references
[1]"Cloning and sequence determination of cDNA encoding a second rat liver peroxisomal 3-ketoacyl-CoA thiolase."
Bodnar A.G., Rachubinski R.A.
Gene 91:193-199(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: Sprague-Dawley.
[2]"Rat peroxisomal 3-ketoacyl-CoA thiolase gene. Occurrence of two closely related but differentially regulated genes."
Hijikata M., Wen J.K., Osumi T., Hashimoto T.
J. Biol. Chem. 265:4600-4606(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Lung.
[4]"A novel, cleavable peroxisomal targeting signal at the amino-terminus of the rat 3-ketoacyl-CoA thiolase."
Swinkels B.W., Gould S.J., Bodnar A.G., Rachubinski R.A., Subramani S.
EMBO J. 10:3255-3262(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: TRANSIT PEPTIDE CLEAVAGE SITE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M32801 mRNA. Translation: AAA41471.1. Different initiation.
D90058 Genomic DNA. Translation: BAA14106.1. Different initiation.
BC089821 mRNA. Translation: AAH89821.1. Different initiation.
PIRXURTAA. A35725.
RefSeqNP_036621.1. NM_012489.2.
UniGeneRn.8913.

3D structure databases

ProteinModelPortalP21775.
SMRP21775. Positions 31-424.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000041776.

PTM databases

PhosphoSiteP21775.

Proteomic databases

PaxDbP21775.
PRIDEP21775.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24157.
KEGGrno:24157.

Organism-specific databases

CTD113868.
RGD67379. Acaa1a.

Phylogenomic databases

eggNOGCOG0183.
HOGENOMHOG000012239.
HOVERGENHBG003112.
InParanoidP21775.
KOK07513.
PhylomeDBP21775.

Enzyme and pathway databases

SABIO-RKP21775.
UniPathwayUPA00199.

Gene expression databases

GenevestigatorP21775.

Family and domain databases

Gene3D3.40.47.10. 4 hits.
InterProIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PANTHERPTHR18919. PTHR18919. 1 hit.
PfamPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
TIGRFAMsTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio602445.
PROP21775.

Entry information

Entry nameTHIKA_RAT
AccessionPrimary (citable) accession number: P21775
Secondary accession number(s): Q5FVR9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: August 30, 2005
Last modified: April 16, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways