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Protein

Outer dense fiber protein 1

Gene

Odf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the outer dense fibers (ODF) of spermatozoa. ODF are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail.

GO - Molecular functioni

  • protein domain specific binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Outer dense fiber protein 1
Alternative name(s):
Protein RT7
RTS 5/1
Gene namesi
Name:Odf1
Synonyms:Odf27, Odfp, Rt7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3228. Odf1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Outer dense fiber protein 1PRO_0000058027Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51PhosphoserineCombined sources
Modified residuei10 – 101PhosphoserineCombined sources
Modified residuei64 – 641PhosphoserineCombined sources
Modified residuei87 – 871PhosphoserineCombined sources
Modified residuei108 – 1081PhosphoserineCombined sources
Modified residuei109 – 1091PhosphoserineCombined sources
Modified residuei137 – 1371PhosphoserineCombined sources
Modified residuei153 – 1531PhosphoserineCombined sources
Modified residuei175 – 1751PhosphoserineCombined sources
Modified residuei180 – 1801PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21769.

PTM databases

iPTMnetiP21769.
PhosphoSiteiP21769.

Expressioni

Tissue specificityi

Testis. Specifically located to the round spermatid layer and to the luminally-oriented cytoplasm of elongated spermatids.

Developmental stagei

First detected in 30-day old rats after which, levels increase during spermatid elongation. Levels decrease at the time of spermatid assembly and disappear just before spermiation.

Gene expression databases

GenevisibleiP21769. RN.

Interactioni

Subunit structurei

Interacts with SPAG4 and KLC3.2 Publications

GO - Molecular functioni

  • protein domain specific binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008700.

Structurei

3D structure databases

ProteinModelPortaliP21769.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati34 – 3851
Repeati74 – 7852

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 78452 X 5 AA repeats of [RC]-C-L-C-DAdd
BLAST
Regioni195 – 23339C-X-P repeat regionAdd
BLAST

Domaini

The C-terminal contains many C-X-P repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IHKT. Eukaryota.
ENOG4111C5F. LUCA.
GeneTreeiENSGT00440000038909.
HOGENOMiHOG000290650.
HOVERGENiHBG007863.
InParanoidiP21769.
OMAiCYPCASP.
OrthoDBiEOG7MD4RJ.
PhylomeDBiP21769.
TreeFamiTF337986.

Family and domain databases

InterProiIPR002068. A-crystallin/Hsp20_dom.
IPR008978. HSP20-like_chaperone.
[Graphical view]
PfamiPF00011. HSP20. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.

Sequencei

Sequence statusi: Complete.

P21769-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALSCLLDS VRRDIKKVDR ELRQLRCIDE ISSRCLCDLY MHPYCCCDLH
60 70 80 90 100
PYPYCLCYSK RSRSCGLCDL YYPCCLCDYK LYCLRPSLRS LERLRRTTNR
110 120 130 140 150
ILASSCCSSN ILGSVNVCGF EPDQVKVRVK DGKVCVSAER ENRYDCLGSK
160 170 180 190 200
KYSYMNICKE FSLPPCVDEK DVTYSYGLGS CVKIESPCYP CTSPCNPCNP
210 220 230 240
CSPCSPCGPC GPCGPCGPCG PCGPCDPCNP CYPCGSRFSC RKMIL
Length:245
Mass (Da):27,351
Last modified:November 1, 1997 - v2
Checksum:i3111C776213A7381
GO

Sequence cautioni

The sequence AAA42091.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 903LRS → SAA in AAA42086 (PubMed:1699827).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88759 mRNA. Translation: AAA03066.1.
M88762 Genomic DNA. Translation: AAA42091.1. Different initiation.
M88762 Genomic DNA. Translation: AAA42092.1. Sequence problems.
BC078708 mRNA. Translation: AAH78708.1.
U09021 mRNA. Translation: AAA67872.1.
M58677 mRNA. Translation: AAA42086.1.
PIRiA43905.
RefSeqiNP_077040.2. NM_024126.5.
UniGeneiRn.9850.

Genome annotation databases

EnsembliENSRNOT00000008700; ENSRNOP00000008700; ENSRNOG00000006578.
GeneIDi24610.
KEGGirno:24610.
UCSCiRGD:3228. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88759 mRNA. Translation: AAA03066.1.
M88762 Genomic DNA. Translation: AAA42091.1. Different initiation.
M88762 Genomic DNA. Translation: AAA42092.1. Sequence problems.
BC078708 mRNA. Translation: AAH78708.1.
U09021 mRNA. Translation: AAA67872.1.
M58677 mRNA. Translation: AAA42086.1.
PIRiA43905.
RefSeqiNP_077040.2. NM_024126.5.
UniGeneiRn.9850.

3D structure databases

ProteinModelPortaliP21769.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008700.

PTM databases

iPTMnetiP21769.
PhosphoSiteiP21769.

Proteomic databases

PaxDbiP21769.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008700; ENSRNOP00000008700; ENSRNOG00000006578.
GeneIDi24610.
KEGGirno:24610.
UCSCiRGD:3228. rat.

Organism-specific databases

CTDi4956.
RGDi3228. Odf1.

Phylogenomic databases

eggNOGiENOG410IHKT. Eukaryota.
ENOG4111C5F. LUCA.
GeneTreeiENSGT00440000038909.
HOGENOMiHOG000290650.
HOVERGENiHBG007863.
InParanoidiP21769.
OMAiCYPCASP.
OrthoDBiEOG7MD4RJ.
PhylomeDBiP21769.
TreeFamiTF337986.

Miscellaneous databases

PROiP21769.

Gene expression databases

GenevisibleiP21769. RN.

Family and domain databases

InterProiIPR002068. A-crystallin/Hsp20_dom.
IPR008978. HSP20-like_chaperone.
[Graphical view]
PfamiPF00011. HSP20. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and developmental expression of a mRNA encoding a putative protein of rat sperm outer dense fibers."
    Burfeind P., Hoyer-Fender S.
    Dev. Biol. 148:195-204(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "Structure and chromosomal assignment of a gene encoding the major protein of rat sperm outer dense fibres."
    Burfeind P., Belgardt B., Szpirer C., Hoyer-Fender S.
    Eur. J. Biochem. 216:497-505(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Wistar.
    Tissue: Spleen and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. "Molecular cloning and developmental expression of an mRNA encoding the 27 kDa outer dense fiber protein of rat spermatozoa."
    Morales C.R., Oko R., Clermont Y.
    Mol. Reprod. Dev. 37:229-240(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 81-245, PROTEIN SEQUENCE OF 50-52 AND 156-165.
    Tissue: Testis.
  5. "A new rat gene RT7 is specifically expressed during spermatogenesis."
    van der Hoorn F.A., Tarnasky H.A., Nordeen S.K.
    Dev. Biol. 142:147-154(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-90.
    Tissue: Testis.
  6. "Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1 and localizes to microtubules of manchette and axoneme."
    Shao X., Tarnasky H.A., Lee J.P., Oko R., van der Hoorn F.A.
    Dev. Biol. 211:109-123(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPAG4.
  7. "Association of kinesin light chain with outer dense fibers in a microtubule-independent fashion."
    Bhullar B., Zhang Y., Junco A., Oko R., van der Hoorn F.A.
    J. Biol. Chem. 278:16159-16168(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KLC3.
  8. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; SER-10; SER-64; SER-87; SER-108; SER-109; SER-137; SER-153; SER-175 AND SER-180, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiODFP1_RAT
AccessioniPrimary (citable) accession number: P21769
Secondary accession number(s): Q62652
, Q63390, Q63534, Q63535
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1997
Last modified: June 8, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.