Reviewed, UniProtKB/Swiss-Prot P21765 (GPX5_MOUSE)

Names and origin

Protein names

Recommended name:
    Epididymal secretory glutathione peroxidase
    EC=1.11.1.9
Alternative name(s):
    Epididymis-specific glutathione peroxidase-like protein
      Short name=EGLP
    Major androgen-regulated protein
    arMEP24

Gene names

Name:

Gpx5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. May constitute a glutathionine peroxidase-like protective system against peroxide damage in sperm membrane lipids.
Catalytic activity	2 glutathione + H2O2 = glutathione disulfide + 2 H2O.
Subcellular location	Secreted.
Tissue specificity	Epididymis.
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 221	200	Epididymal secretory glutathione peroxidase		PRO_0000013078
Sites
	Active site	73	1	By similarity
Experimental info
	Sequence conflict	53	1	D → H in CAA37796. Ref.3
	Sequence conflict	103	1	Q → E Ref.2
	Sequence conflict	170	1	S → F Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P21765-1 [UniParc]. Last modified August 1, 1992. Version 2. Checksum: DE5F8BD6CD22D6F9	FASTA	221	25,382
	10 20 30 40 50 60 MVTELRVFYL VPLLLASYVQ TTPRPEKMKM DCYKDVKGTI YDYEALSLNG KEDIPFKQYR 70 80 90 100 110 120 GKHVLFVNVA TYCGLTIQYP ELNALQEDLK PFGLVILGFP CNQFGKQEPG DNLEILPGLK 130 140 150 160 170 180 YVRPGKGFLP NFQLFAKGDV NGENEQKIFT FLKRSCPHPS ETVVMSKHTS WEPIKVHDIR 190 200 210 220 WNFEKFLVGP DGVPVMRWFH QAPVSTVKSD IMAYLSHFKT I

References

[1]	"Androgen-dependent protein secreted by mouse caput epididymis shows high homologies with different glutathione peroxidases." Ghyselinck N.B., Rigaudiere N., Dufaure J.-P. C. R. Acad. Sci. III, Sci. Vie 313:1-6(1991) [PubMed: 1913244] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Epididymis.
[2]	"Structural organization and regulation of the gene for the androgen-dependent glutathione peroxidase-like protein specific to the mouse epididymis." Ghyselinck N.B., Dufaure I., Lareyre J.J., Rigaudiere N., Mattei M.-G., Dufaure J.-P. Mol. Endocrinol. 7:258-272(1993) [PubMed: 8469239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A mouse cDNA sequence for epididymal androgen-regulated proteins related to glutathione peroxidase." Ghyselinck N.B., Dufaure J.-P. Nucleic Acids Res. 18:7144-7144(1990) [PubMed: 2263479] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 47-221. Tissue: Epididymis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M68896 Genomic DNA. Translation: AAA37729.1. X53780 mRNA. Translation: CAA37796.1.
IPI	IPI00127653.
PIR	A47367.
UniGene	Mm.1332
3D structure databases
HSSP	HSSP built from PDB template 1GP1 based on UniProtKB P00435.
ModBase	Search...
Protein-protein interaction databases
STRING	P21765.
Protein family/group databases
PeroxiBase	5578. MmGPx05.
Proteomic databases
PRIDE	P21765.
Genome annotation databases
Ensembl	ENSMUST00000004456; ENSMUSP00000004456; ENSMUSG00000004344; Mus musculus. [Genome view] ENSMUST00000110491; ENSMUSP00000106117; ENSMUSG00000004344; Mus musculus. [Genome view]
UCSC	uc007ppz.1. mouse.
Organism-specific databases
MGI	MGI:104886. Gpx5.
Phylogenomic databases
HOGENOM	P21765.
HOVERGEN	P21765.
Enzyme and pathway databases
BRENDA	1.11.1.9. 244.
Gene expression databases
ArrayExpress	P21765.
Bgee	P21765.
CleanEx	MM_GPX5.
Genevestigator	P21765.
GermOnline	ENSMUSG00000004344. Mus musculus.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

GPX5_MOUSE

Accession

Primary (citable) accession number: P21765

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1991
Last sequence update:	August 1, 1992
Last modified:	October 13, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)