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Reviewed, UniProtKB/Swiss-Prot P21764 (LIG3_PHACH)

Last modified May 5, 2009. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ligninase LG3
    EC=1.11.1.14
Alternative name(s):
    Diarylpropane peroxidase
    Lignin peroxidase
Gene names
Name: GLG3
Synonyms: LIP
OrganismPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifier5306 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Corticiales › Corticiaceae › Phanerochaete

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Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Depolymerization of lignin. Catalyzes the C(alpha)-C(beta) cleavage of the propyl side chains of lignin.

Catalytic activity

1,2-bis(3,4-dimethoxyphenyl)propane-1,3-diol + H2O2 = 3,4-dimethoxybenzaldehyde + 1-(3,4-dimethoxyphenyl)ethane-1,2-diol + H2O.

Cofactor

Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity.

Binds 2 calcium ions per subunit By similarity.

Pathway

Secondary metabolite metabolism; lignin degradation.

Developmental stage

Ligninases are expressed during secondary metabolism, and are triggered by nutrient limitation.

Sequence similarities

Belongs to the peroxidase family. Ligninase subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Propeptide22 – 287
PRO_0000023764
Chain29 – 372344Ligninase LG3
PRO_0000023765

Sites

Active site751Proton acceptor By similarity
Metal binding761Calcium 1 By similarity
Metal binding941Calcium 1; via carbonyl oxygen By similarity
Metal binding961Calcium 1 By similarity
Metal binding981Calcium 1 By similarity
Metal binding2041Iron (heme axial ligand) By similarity
Metal binding2051Calcium 2 By similarity
Metal binding2221Calcium 2 By similarity
Metal binding2241Calcium 2 By similarity
Metal binding2271Calcium 2; via carbonyl oxygen By similarity
Metal binding2291Calcium 2 By similarity
Site711Transition state stabilizer By similarity

Amino acid modifications

Glycosylation2851N-linked (GlcNAc...) Potential
Disulfide bond31 ↔ 43 By similarity
Disulfide bond62 ↔ 148 By similarity
Disulfide bond277 ↔ 345 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P21764-1 [UniParc].

Last modified May 1, 1991. Version 1.
Checksum: DA0293178F4E2A4A

FASTA37239,536
        10         20         30         40         50         60 
MAFKQLFAAI SLALSLSAAN AAAVIEKRAT CSNGKTVGDA SSCAWFDVLD DIQQNLFHGG 

        70         80         90        100        110        120 
QCGAEAHESI RLVFHDSIAI SPAMEAQGKF GGGGADGSIM IFDDIETAFH PNIGLDEIVK 

       130        140        150        160        170        180 
LQKPFVQKHG CTPGDFIAFA GAVALSNCPG APQMNFFTGR APATQAAPDG LVPEPFHTVD 

       190        200        210        220        230        240 
QIINRVNDAG EFDELELVWM LSAHSVAAVN DVDPTVQGLP FDSTPGIFDS QFFVETQLRG 

       250        260        270        280        290        300 
TAFPGSGGNQ GEVESPLPGE IRIQSDHTIA RDSRTACEWQ SFVNNQSKLV DDFQFIFLAL 

       310        320        330        340        350        360 
TQLGQDPNAM TDCSDVIPQS KPIPGNLPFS FFPAGKTIKD VEQACAETPF PTLTTLPGPE 

       370 
TSVQRIPPPP GA

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References

[1]"Nucleotide sequence of a new lignin peroxidase gene GLG3 from the white-rot fungus, Phanerochaete chrysosporium."
Naidu P.S., Reddy C.A.
Nucleic Acids Res. 18:7173-7173(1990) [PubMed: 2129560] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 24725 / CBS 481.73 / CCRC 36200 / NRRL 6361 / VKM-F-1767.

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Cross-references

Sequence databases

X51590 Genomic DNA. Translation: CAA35939.1.
PIRA32322.
B32322.
PS0010.
OPJG3P. S13723.
S69246.

3D structure databases

HSSPHSSP built from PDB template 1B80 based on UniProtKB P06181.
SMRP21764. Positions 24-372.
ModBaseSearch...

Protein family/group databases

PeroxiBase2482. PcLiPI.

Enzyme and pathway databases

BRENDA1.11.1.14. 16698.

Family and domain databases

InterProIPR002016. Haem_peroxidase_pln/fun/bac.
IPR001621. Ligninase.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSPR00462. LIGNINASE.
PR00458. PEROXIDASE.
PROSITEPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameLIG3_PHACH
AccessionPrimary (citable) accession number: P21764
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 1991
Last modified: May 5, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents