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P21763

- T2R1_RHOSH

UniProt

P21763 - T2R1_RHOSH

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Protein
Type-2 restriction enzyme RsrI
Gene
rsrIR
Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GAATTC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Magnesium.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC
  3. magnesium ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Protein family/group databases

REBASEi1572. RsrI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme RsrI (EC:3.1.21.4)
Short name:
R.RsrI
Alternative name(s):
Endonuclease RsrI
Type II restriction enzyme RsrI
Gene namesi
Name:rsrIR
OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifieri1063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 277276Type-2 restriction enzyme RsrI
PRO_0000077357Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP21763.
SMRiP21763. Positions 24-275.

Family & Domainsi

Family and domain databases

Gene3Di3.40.580.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR004221. Restrct_endonuc_II_EcoRI.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR018131. Restrct_endonuc_II_EcoRI_Pbac.
[Graphical view]
PfamiPF02963. EcoRI. 1 hit.
[Graphical view]
PIRSFiPIRSF001002. Restrict_endonuc_II_EcoRI. 1 hit.
ProDomiPD039825. Restrict_endonuc_II_EcoRI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21763-1 [UniParc]FASTAAdd to Basket

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MAGEVEFKGK GQALRLGIQQ ELGGGPLSIF GAAAQKHDLS IREVTAGVLT    50
KLAEDFPNLE FQLRTSLTKK AINEKLRSFD PRLGQALFVE SASIRPDGGI 100
TEVKDRHGNW RVILVGESKH QGNDVEKILA GVLQGKAKDQ DFMAAGNAIE 150
RMHKNVLELR NYMLDEKHFP YVVFLQGSNF ATESFEVTRP DGRVVKIVHD 200
SGMLNRIDRV TASSLSREIN QNYCENIVVR AGSFDHMFQI ASLYCKAAPW 250
TAGEMAEAML AVAKTSLRII ADDLDQN 277
Length:277
Mass (Da):30,630
Last modified:January 23, 2007 - v3
Checksum:i672E812D258E70B2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14697 Genomic DNA. Translation: CAA32827.1.
PIRiJW0016.
S03688.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14697 Genomic DNA. Translation: CAA32827.1 .
PIRi JW0016.
S03688.

3D structure databases

ProteinModelPortali P21763.
SMRi P21763. Positions 24-275.
ModBasei Search...

Protein family/group databases

REBASEi 1572. RsrI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.580.10. 1 hit.
InterProi IPR011335. Restrct_endonuc-II-like.
IPR004221. Restrct_endonuc_II_EcoRI.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR018131. Restrct_endonuc_II_EcoRI_Pbac.
[Graphical view ]
Pfami PF02963. EcoRI. 1 hit.
[Graphical view ]
PIRSFi PIRSF001002. Restrict_endonuc_II_EcoRI. 1 hit.
ProDomi PD039825. Restrict_endonuc_II_EcoRI. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparison of the nucleotide and amino acid sequences of the RsrI and EcoRI restriction endonucleases."
    Stephenson F.H., Ballard B.T., Boyer H.W., Rosenberg J.M., Greene P.J.
    Gene 85:1-13(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-35.
  2. "Restriction endonuclease RsrI from Rhodobacter sphaeroides, an isoschizomer of EcoRI: purification and properties."
    Aiken C., Gumport R.I.
    Nucleic Acids Res. 16:7901-7916(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-41.

Entry informationi

Entry nameiT2R1_RHOSH
AccessioniPrimary (citable) accession number: P21763
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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