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Protein

Type-2 restriction enzyme RsrI

Gene

rsrIR

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GAATTC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandMagnesium

Protein family/group databases

REBASEi1572. RsrI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme RsrI (EC:3.1.21.4)
Short name:
R.RsrI
Alternative name(s):
Endonuclease RsrI
Type II restriction enzyme RsrI
Gene namesi
Name:rsrIR
OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifieri1063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000773572 – 277Type-2 restriction enzyme RsrIAdd BLAST276

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP21763.
SMRiP21763.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

Gene3Di3.40.580.10. 1 hit.
InterProiView protein in InterPro
IPR011335. Restrct_endonuc-II-like.
IPR004221. Restrct_endonuc_II_EcoRI.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR018131. Restrct_endonuc_II_EcoRI_Pbac.
PfamiView protein in Pfam
PF02963. EcoRI. 1 hit.
PIRSFiPIRSF001002. Restrict_endonuc_II_EcoRI. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD039825. Restrict_endonuc_II_EcoRI. 1 hit.
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21763-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGEVEFKGK GQALRLGIQQ ELGGGPLSIF GAAAQKHDLS IREVTAGVLT
60 70 80 90 100
KLAEDFPNLE FQLRTSLTKK AINEKLRSFD PRLGQALFVE SASIRPDGGI
110 120 130 140 150
TEVKDRHGNW RVILVGESKH QGNDVEKILA GVLQGKAKDQ DFMAAGNAIE
160 170 180 190 200
RMHKNVLELR NYMLDEKHFP YVVFLQGSNF ATESFEVTRP DGRVVKIVHD
210 220 230 240 250
SGMLNRIDRV TASSLSREIN QNYCENIVVR AGSFDHMFQI ASLYCKAAPW
260 270
TAGEMAEAML AVAKTSLRII ADDLDQN
Length:277
Mass (Da):30,630
Last modified:January 23, 2007 - v3
Checksum:i672E812D258E70B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14697 Genomic DNA. Translation: CAA32827.1.
PIRiJW0016.
S03688.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiT2R1_RHOSH
AccessioniPrimary (citable) accession number: P21763
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: February 15, 2017
This is version 85 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries