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P21763 (T2R1_RHOSH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme RsrI

Short name=R.RsrI
EC=3.1.21.4
Alternative name(s):
Endonuclease RsrI
Type II restriction enzyme RsrI
Gene names
Name:rsrIR
OrganismRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifier1063 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length277 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GAATTC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Subunit structure

Homodimer.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1 Ref.2
Chain2 – 277276Type-2 restriction enzyme RsrI
PRO_0000077357

Sequences

Sequence LengthMass (Da)Tools
P21763 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 672E812D258E70B2

FASTA27730,630
        10         20         30         40         50         60 
MAGEVEFKGK GQALRLGIQQ ELGGGPLSIF GAAAQKHDLS IREVTAGVLT KLAEDFPNLE 

        70         80         90        100        110        120 
FQLRTSLTKK AINEKLRSFD PRLGQALFVE SASIRPDGGI TEVKDRHGNW RVILVGESKH 

       130        140        150        160        170        180 
QGNDVEKILA GVLQGKAKDQ DFMAAGNAIE RMHKNVLELR NYMLDEKHFP YVVFLQGSNF 

       190        200        210        220        230        240 
ATESFEVTRP DGRVVKIVHD SGMLNRIDRV TASSLSREIN QNYCENIVVR AGSFDHMFQI 

       250        260        270 
ASLYCKAAPW TAGEMAEAML AVAKTSLRII ADDLDQN 

« Hide

References

[1]"Comparison of the nucleotide and amino acid sequences of the RsrI and EcoRI restriction endonucleases."
Stephenson F.H., Ballard B.T., Boyer H.W., Rosenberg J.M., Greene P.J.
Gene 85:1-13(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-35.
[2]"Restriction endonuclease RsrI from Rhodobacter sphaeroides, an isoschizomer of EcoRI: purification and properties."
Aiken C., Gumport R.I.
Nucleic Acids Res. 16:7901-7916(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-41.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14697 Genomic DNA. Translation: CAA32827.1.
PIRJW0016.
S03688.

3D structure databases

ProteinModelPortalP21763.
SMRP21763. Positions 24-275.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1572. RsrI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.580.10. 1 hit.
InterProIPR011335. Restrct_endonuc-II-like.
IPR004221. Restrct_endonuc_II_EcoRI.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR018131. Restrct_endonuc_II_EcoRI_Pbac.
[Graphical view]
PfamPF02963. EcoRI. 1 hit.
[Graphical view]
PIRSFPIRSF001002. Restrict_endonuc_II_EcoRI. 1 hit.
ProDomPD039825. Restrict_endonuc_II_EcoRI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2R1_RHOSH
AccessionPrimary (citable) accession number: P21763
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries