Reviewed,
UniProtKB/Swiss-Prot P21763 (T2R1_RHOSH)
Last modified
November 3, 2009.
Version 60.
History...
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90%,
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Type-2 restriction enzyme RsrI Short name=R.RsrI EC=3.1.21.4 Alternative name(s): Type II restriction enzyme RsrI Endonuclease RsrI | ||
| Gene names |
| ||
| Organism | Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides) | ||
| Taxonomic identifier | 1063 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter |
Protein attributes
| Sequence length | 277 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Recognizes the double-stranded sequence GAATTC and cleaves after G-1. |
| Catalytic activity | Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates. |
| Cofactor | Magnesium. |
| Subunit structure | Homodimer. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Restriction system |
| Ligand | Magnesium |
| Molecular function | Endonuclease Hydrolase Nuclease |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activityInferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| [1] | "Comparison of the nucleotide and amino acid sequences of the RsrI and EcoRI restriction endonucleases." Stephenson F.H., Ballard B.T., Boyer H.W., Rosenberg J.M., Greene P.J. Gene 85:1-13(1989) [PubMed: 2695392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-35. |
| [2] | "Restriction endonuclease RsrI from Rhodobacter sphaeroides, an isoschizomer of EcoRI: purification and properties." Aiken C., Gumport R.I. Nucleic Acids Res. 16:7901-7916(1988) [PubMed: 2843805] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-41. |
Cross-references
Sequence databases | |
|---|---|
| X14697 Genomic DNA. Translation: CAA32827.1. | |
| PIR | JW0016. S03688. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1CKQ based on UniProtKB P00642. |
| ModBase | Search... |
Protein family/group databases | |
| REBASE | 1572. RsrI. |
Enzyme and pathway databases | |
| BRENDA | 3.1.21.4. 2796. |
Family and domain databases | |
| InterPro | IPR004221. Restrict_endonuc_II_EcoRI. IPR011336. Restrict_endonuc_II_EcoRI/MunI. IPR018131. Restrict_endonuc_II_EcoRI_Pbac. [Graphical view] |
| Gene3D | G3DSA:3.40.580.10. Restrict_endonuc_II_EcoRI/MunI. 1 hit. |
| Pfam | PF02963. EcoRI. 1 hit. [Graphical view] |
| PIRSF | PIRSF001002. Restrict_endonuc_II_EcoRI. 1 hit. |
| ProDom | PD039825. EcoRI. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| ProtoNet | Search... |
Entry information
| Entry name | T2R1_RHOSH | ||||||||
| Accession | Primary (citable) accession number: P21763 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Restriction enzymes and methylases Classification of restriction enzymes and methylases and list of entries |

Clusters with


