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P21763

- T2R1_RHOSH

UniProt

P21763 - T2R1_RHOSH

Protein

Type-2 restriction enzyme RsrI

Gene

rsrIR

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GAATTC and cleaves after G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. magnesium ion binding Source: InterPro
    3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Protein family/group databases

    REBASEi1572. RsrI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme RsrI (EC:3.1.21.4)
    Short name:
    R.RsrI
    Alternative name(s):
    Endonuclease RsrI
    Type II restriction enzyme RsrI
    Gene namesi
    Name:rsrIR
    OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
    Taxonomic identifieri1063 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 277276Type-2 restriction enzyme RsrIPRO_0000077357Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP21763.
    SMRiP21763. Positions 24-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.580.10. 1 hit.
    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR004221. Restrct_endonuc_II_EcoRI.
    IPR011336. Restrct_endonuc_II_EcoRI/MunI.
    IPR018131. Restrct_endonuc_II_EcoRI_Pbac.
    [Graphical view]
    PfamiPF02963. EcoRI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001002. Restrict_endonuc_II_EcoRI. 1 hit.
    ProDomiPD039825. Restrict_endonuc_II_EcoRI. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P21763-1 [UniParc]FASTAAdd to Basket

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    MAGEVEFKGK GQALRLGIQQ ELGGGPLSIF GAAAQKHDLS IREVTAGVLT    50
    KLAEDFPNLE FQLRTSLTKK AINEKLRSFD PRLGQALFVE SASIRPDGGI 100
    TEVKDRHGNW RVILVGESKH QGNDVEKILA GVLQGKAKDQ DFMAAGNAIE 150
    RMHKNVLELR NYMLDEKHFP YVVFLQGSNF ATESFEVTRP DGRVVKIVHD 200
    SGMLNRIDRV TASSLSREIN QNYCENIVVR AGSFDHMFQI ASLYCKAAPW 250
    TAGEMAEAML AVAKTSLRII ADDLDQN 277
    Length:277
    Mass (Da):30,630
    Last modified:January 23, 2007 - v3
    Checksum:i672E812D258E70B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14697 Genomic DNA. Translation: CAA32827.1.
    PIRiJW0016.
    S03688.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14697 Genomic DNA. Translation: CAA32827.1 .
    PIRi JW0016.
    S03688.

    3D structure databases

    ProteinModelPortali P21763.
    SMRi P21763. Positions 24-275.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1572. RsrI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.580.10. 1 hit.
    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR004221. Restrct_endonuc_II_EcoRI.
    IPR011336. Restrct_endonuc_II_EcoRI/MunI.
    IPR018131. Restrct_endonuc_II_EcoRI_Pbac.
    [Graphical view ]
    Pfami PF02963. EcoRI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001002. Restrict_endonuc_II_EcoRI. 1 hit.
    ProDomi PD039825. Restrict_endonuc_II_EcoRI. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparison of the nucleotide and amino acid sequences of the RsrI and EcoRI restriction endonucleases."
      Stephenson F.H., Ballard B.T., Boyer H.W., Rosenberg J.M., Greene P.J.
      Gene 85:1-13(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-35.
    2. "Restriction endonuclease RsrI from Rhodobacter sphaeroides, an isoschizomer of EcoRI: purification and properties."
      Aiken C., Gumport R.I.
      Nucleic Acids Res. 16:7901-7916(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-41.

    Entry informationi

    Entry nameiT2R1_RHOSH
    AccessioniPrimary (citable) accession number: P21763
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 79 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3