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Protein

Type-2 restriction enzyme RsrI

Gene

rsrIR

Organism
Rhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GAATTC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. magnesium ion binding Source: InterPro
  3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Protein family/group databases

REBASEi1572. RsrI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme RsrI (EC:3.1.21.4)
Short name:
R.RsrI
Alternative name(s):
Endonuclease RsrI
Type II restriction enzyme RsrI
Gene namesi
Name:rsrIR
OrganismiRhodobacter sphaeroides (Rhodopseudomonas sphaeroides)
Taxonomic identifieri1063 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 277276Type-2 restriction enzyme RsrIPRO_0000077357Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP21763.
SMRiP21763. Positions 24-275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

Gene3Di3.40.580.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR004221. Restrct_endonuc_II_EcoRI.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR018131. Restrct_endonuc_II_EcoRI_Pbac.
[Graphical view]
PfamiPF02963. EcoRI. 1 hit.
[Graphical view]
PIRSFiPIRSF001002. Restrict_endonuc_II_EcoRI. 1 hit.
ProDomiPD039825. Restrict_endonuc_II_EcoRI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21763-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGEVEFKGK GQALRLGIQQ ELGGGPLSIF GAAAQKHDLS IREVTAGVLT
60 70 80 90 100
KLAEDFPNLE FQLRTSLTKK AINEKLRSFD PRLGQALFVE SASIRPDGGI
110 120 130 140 150
TEVKDRHGNW RVILVGESKH QGNDVEKILA GVLQGKAKDQ DFMAAGNAIE
160 170 180 190 200
RMHKNVLELR NYMLDEKHFP YVVFLQGSNF ATESFEVTRP DGRVVKIVHD
210 220 230 240 250
SGMLNRIDRV TASSLSREIN QNYCENIVVR AGSFDHMFQI ASLYCKAAPW
260 270
TAGEMAEAML AVAKTSLRII ADDLDQN
Length:277
Mass (Da):30,630
Last modified:January 22, 2007 - v3
Checksum:i672E812D258E70B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14697 Genomic DNA. Translation: CAA32827.1.
PIRiJW0016.
S03688.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14697 Genomic DNA. Translation: CAA32827.1.
PIRiJW0016.
S03688.

3D structure databases

ProteinModelPortaliP21763.
SMRiP21763. Positions 24-275.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1572. RsrI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.580.10. 1 hit.
InterProiIPR011335. Restrct_endonuc-II-like.
IPR004221. Restrct_endonuc_II_EcoRI.
IPR011336. Restrct_endonuc_II_EcoRI/MunI.
IPR018131. Restrct_endonuc_II_EcoRI_Pbac.
[Graphical view]
PfamiPF02963. EcoRI. 1 hit.
[Graphical view]
PIRSFiPIRSF001002. Restrict_endonuc_II_EcoRI. 1 hit.
ProDomiPD039825. Restrict_endonuc_II_EcoRI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparison of the nucleotide and amino acid sequences of the RsrI and EcoRI restriction endonucleases."
    Stephenson F.H., Ballard B.T., Boyer H.W., Rosenberg J.M., Greene P.J.
    Gene 85:1-13(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-35.
  2. "Restriction endonuclease RsrI from Rhodobacter sphaeroides, an isoschizomer of EcoRI: purification and properties."
    Aiken C., Gumport R.I.
    Nucleic Acids Res. 16:7901-7916(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-41.

Entry informationi

Entry nameiT2R1_RHOSH
AccessioniPrimary (citable) accession number: P21763
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 1991
Last sequence update: January 22, 2007
Last modified: November 25, 2014
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.